The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Antiviral Effects of the Culture Filtrate from Serratia marcescens Gsm01, against Cucumber mosaic virus (CMV)

  • Thapa, Shree Prasad (Division of Biological Environment, Kangwon National University) ;
  • Lee, Hye-Jin (Division of Bio-resources Technology, Kangwon National University) ;
  • Park, Duck-Hwan (Department of Forest Resources Protection, Kangwon National University) ;
  • Kim, Sam-Kyu (Division of Biological Environment, Kangwon National University) ;
  • Cho, Jun-Mo (Division of Biological Environment, Kangwon National University) ;
  • Cho, Sae-Youll (Division of Bio-resources Technology, Kangwon National University) ;
  • Hur, Jang-Hyun (Division of Biological Environment, Kangwon National University) ;
  • Lim, Chun-Keun (Division of Bio-resources Technology, Kangwon National University)
  • Published : 2009.12.01
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Abstract

The potential antiviral effects of the culture filtrates (CF) from Serratia marcescens strain Gsm01 against yellow strain of Cucumber mosaic virus (CMV-Y) were investigated. The culture filtrate of S. marcescens strain Gsm01 applied on Chenopodium amaranticolor showed high inhibitory activity, likewise no necrosis appeared when applied on the tobacco plants 2 days before CMV-Y inoculation. When plants were challenge inoculated with CMV-Y for eighteen days, the disease incidence in plants with culture filtrate of S. marcescens Gsm01 did not exceed 59%, whereas 100% of control plants were severely infected. The results of double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA), reverse transcriptase polymerase chain reaction (RT-PCR), dot blotting, and western blotting showed that culture filtrate treatment highly affected the accumulation of CMV-Y or its CP protein gene in the treated plant leaves. It was also observed that the culture filtrate had no RNase activity on genomic RNAs of CMV-Y, suggesting that culture filtrate may not contain ribosome inactivating proteins (RIPs) or proteins with RNase activity. These data shows that culture filtrate of S. marcescens strain Gsm01 seems to be a promising source of antiviral substance for the practical use.

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References

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