• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.04a
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• pp.61-62
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• 2003

Clonal propagation of high-value forest trees through somatic embryogenesis (SE) has the potential to rapidly capture the benefits of breeding or genetic engineering programs and to improve raw material uniformity and quality. A major barrier to the commercialization of this technology is the low quality of the resulting embryos. Several factors limit commercialization of SE for Corsican pine, including low initiation rates, low culture survival, culture decline causing low or no embryo production, and inability of somatic embryos to fully mature, resulting in low germination and reduced vigour of somatic seedlings. The objective was to develop a Corsican pine maturation medium that would produce cotyledonary embryos capable of germination. Treatments were arranged in a completely randomized design. Data were analyzed by analysis of variance, and significant differences between treatments determined by multiple range test at P=0.05. Corsican pine (Pinus nigra var. maritima) cultures were initiated on modified !P6 medium. Modifications of the same media were used for culture multiplication and maintenance. Embryogenic cultures were maintained on the same medium semi solidified with 2.5 g/l Gelrite. A maturation medium, capable of promoting the development of Corsican pine somatic embryos that can germinate, is a combination of iP6 modified salts, 2% maltose, 13% polyethylene glycol (PEG), 5 mg!l abscisic acid (ABA), and 2.5 g/l Gelrite. After initiation and once enough tissue developed they were grown in liquid medium. Embryogenic cell suspensions were established by adding 0.951.05 g of 10- to 14-day-old semisolid-grown embryogenic tissue to 9 ml of liquid maintenance media in a 250ml Erlenmeyer flask. Cultures were then incubated in the dark at 2022$^{\circ}$C and rotated at 120 rpm. After 2.53 months on maturation medium, somatic embryos were selected that exhibited normal embryo shape. Ten embryos were placed horizontally on 20 ml of either germination medium ($\frac{2}{1}$strength Murashige and Skoog (1962) salts with 2.5 g/l activated charcoal) or same medium with copper sulphate adjusted to 0.25 mg/1 to compensate for copper adsorption by activated carbon. 2% and 4% maltose was substituted by 7.5% and 13% PEG respectively to improve the yield of the embryos. Substitution of' maltose with PEG was clearly beneficial to embryo development. When 2% of the maltose was replaced with 7.5% PEG, many embryos developed to large bullet-shaped embryos. At latter stages of development most embryos callused and stopped development. A few short, barrel-shaped cotyledonary embryos formed that were covered by callus on the sides and base. When 4% of the maltose was removed and substituted with 13% PEG, the embryos developed further, emerging from the callus and increasing yield slightly. Microscopic examination of the cultures showed differing morphologies, varying from mostly single cells or clumps to well-formed somatic embryos that resembled early zygotic embryos only liquid cultures with organized early-stag. A procedure for converting and acclimating germinants to growth in soil and greenhouse conditions is also tested. Seedling conversion and growth were highly related to the quality of the germinant at the time of planting. Germinants with larger shoots, longer, straighter hypocotyls and longer roots performed best. When mature zygotic embryos germinate the root emerges, before or coincident with the shoot. In contrast, somatic embryos germinate in reverse sequence, with the cotyledons greening first, then shoot emergence and then, much later, if at all, the appearance of the root. Somatic seedlings, produced from the maturation medium, showed 100% survival when planted in a field setting. Somatic seedlings showed normal yearly growth relative to standard seedlings from natural seed.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.2
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• pp.137-152
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• 2009

Present experiment was conducted at the field of Gyeongnam Agricultural Research and Extension Services in Jinju city for two continuous cropping seasons to develop several adaptable and valuable year-round forage-producing system for elevating self-sufficiency and dollar-saving by reduced importing of crude forage. Twenty cropping systems were tested in experiment using whole crop barley (WCB), oat, rye, Italian ryegrass (IRG), and triticale in winter season and com, sorghum, sorghum ${\times}$ sudangrass hybrid, and oat in summer time. Sorghum ${\times}$ sudangrass hybrid showed highest fresh forage yield among experimented summer season crops, and followed com. Com produced the most dry matter yield, and followed sorghum${\times}$sudangrass hybrid, sorghum and oat in order. There was no significant effect of former winter crops on fresh and dry matter production succeeding summer time crops. Among winter season forage crops tested, oat showed the highest fresh and dry matter when clipped on mid-May, and followed triticale, IRG, rye and WCB. Winter-time cultivated crops showed no clear effect on the growth and forage (fresh and dry matter) producing ability of following summer crops. There was the most protein content in oat plant among summer season planted crops, and in sorghum for acid detergent fiber (ADF) and in sorghum ${\times}$ sudangrass hybrid for neutral detergent fiber (NDF), respectively. While, com showed highest value of relative feed value (RFV) and total digestive nutrients (TDN) among those crops. Among winter crops, the highest crude protein was in oat plant showing no significant differences of ADF and NDF, while, relatively higher value of RFV was recognized with rye and triticale. Also, triticale contained more TDN as compare to other forage crops. The cropping combinations such as com followed by (fb) rye and maize fb triticale were regarded as promising systems having higher dry matter producing ability among tested combinations. Considering TDN producing potential, the combinations with sorghum ${\times}$ sudangrass hybrid fb triticale andlor rye were would be suitable ones, coincidently. There was a tendency which elevating pH, electric conductivity (EC) and organic matter (OM) contents in soil after experiment comparing to before planting. More crude protein content in plant was shown at mid-May clipping as compared to the forage at April cut in all winter season grown crops. ADF and NDF contents were increased by delayed clipping showing decreased tendency of RFV and TDN in plant. In conclusion, many cropping systems would be available using above mentioned forage crops according to farmer's conditions and scale, etc.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.36 no.3
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• pp.88-99
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• 2018

After UNESCO appointed Royal Tombs of the Joseon Dynasty to be World Cultural Heritages(2009), Cultural Heritage Administration in Korea established Management Office of Royal Tombs of the Joseon Dynasty(2012) and conducted Mid to Long-Term Plan on Conservation, Management, Utilization of World Heritage 'Royal Tombs of the Joseon Dynasty(2015)'. The report, as an implementation plan, provides detailed contents of the maintenance planning of Royal Tombs of the Joseon Dynasty. From this background, with a view to comprehending the characteristics of the maintenance planning of trees in historical landscape forests, this study extracted the contents related to the historical landscape forests of the 40 Royal Tombs of the Joseon Dynasty from the "Mid to Long-term Plan" and conducted literature research. The results of the study are as follows. First, from 2016, the short-term plans have provided detailed contents on logging, transplanting and planting trees in the plans divided by stages. On the other hand, the long-term plans, instead of presenting abstract contents repeatedly for each royal tomb, should seek vegetation landscape that each royal tomb should aim for. Second, since the areas that are in charge of the various functions of the royal tombs have been subjects to the maintenance planning, it is effective to establish a maintenance planning by separating functional areas instead of the comprehensive concept as historical landscape forests. Third, the maintenance planning of trees in historical landscape forest of the Royal Tombs of Joseon Dynasty is based on daily management such as density control and maintaining the shape of trees, which is necessary to sustain the forest. Also, due to the goal of preserving the pine forest, which is recognized as a prototype vegetation landscape, foreign species and other trees have been more cut down. With a few limited types of tall tree but few shrubs and flowers, it is characterized that the orientation toward aesthetic landscapes is insufficient in large green spaces. Fourth, according to the function of the tomb, it is divided into entrance area, ceremonial area, burial area and outer forest, and the contents of the maintenance planning are analyzed by basic conditions of tree management: prototype, ecology, functionality, and aesthetics. After studying the tree management characteristics of each area, four suggestions are made: understanding the diversity of trees that form prototype landscape as a management plan, sustainability of various forests adapted to ecological environment, convenience required in addition to ceremony and safety characterizing the Royal Tombs of Joseon Dynasty, and the necessity of access to aesthetic landscape on the tombs.

• Journal of the Korean Institute of Landscape Architecture
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• v.39 no.1
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• pp.117-123
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• 2011

This study aims to suggest a suitable soil thickness and soil mixture ratio of a green roof system by verifying the growth of Chrysanthemum zawadskii var. coreanum as affected by different green roof systems using rainwater. The experimental planting grounds were made with different soil thicknesses(15cm, 25cm) and soil mixing ratios (SL, $P_7P_1L_2$, $P_6P_2L_2$, $P_5P_3L_2$, $P_4P_4L_2$) and with excellent drought tolerance. Ornamental value Chrysanthemum zawadskii var. coreanum was planted. The change in plant height, green coverage ratio, chlorophyll content, fresh weight, dry weight, and dry T/R ratio of Chrysanthemum zawadskii var. coreanum were investigated from April to October 2009. For 15cm soil thickness, the plant height of Chrysanthemum zawadskii var. coreanum was not significantly different as affected by the soil mixing ratio. However, it was found to be higher in the amended soil mixture, $P_7P_1L_2$, $P_6P_2L_2$, $P_5P_3L_2$ and $P_4P_4L_2$ than in the sandy loam soil, as it was SL overall. For 25cm soil the plant height differences were in order to SL < $P_7P_1L_2$, $P_6P_2L_2$, $P_5P_3L_2$ < $P_4P_4L_2$. The green coverage ratio was observed not to be different by soil mixing ratio with soil thickness of 15cm, but, the lowest green coverage ratio in the SL. In the 25cm soil thickness, the green coverage ratio was 86-89% with a good coverage rate overall. The change in chlorophyll contents with 15cm soil thickness was found to be the highest in the SL treatment and the lowest in the $P_5P_3L_2$ treatment. For 25cm thickness, the highest value was in the $P_4P_4L_2$ and SL, and the lowest in the$P_7P_1L_2$. Fresh weight and dry weight were larger in soil with 25cm thickness. Therefore, the growth of Chrysanthemum zawadskii var. coreanum as affected by a different green roof system for using rainwater was higher in soil with 25cm thickness than 15cm, and in PPL amended soil than in sandy loam.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.31 no.4
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• pp.84-94
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• 2013

This study scrutinizes the titles of serial poems on Japanese Apricot, which have lucid characters on season and time changes, having been appreciated and recited by the scholars in the Choseon Dynasty era and analyses the records of Zhang zi(1153~1235), a writer in Song(宋) Dynasty in China, having presented the objects harmonizing perfectly with Japanese Apricot. The results of this study categorizes the objects of Japanese Apricot appreciation and establishes the landscape characteristics on Japanese Apricot appreciation affiliated with as follows. First, the objects of Japanese Apricot appreciation are categorized into 'form of blossoms', 'natural feature(景物)', 'place of tree planting', 'the picturesque scene(景色)' and 'behavior'. Second, the scholars regarded the single trees whose branches are grotesque as the objects of appreciation and enjoyed them. They preferred white and single petal Japanese Apricot and admired red Japanese Apricot which has Taoism images. Third, they admired pines and camelias which represent fidelity and strength and valued Japanese Apricot with cranes which remind themselves of solitary scholars. Fourth, they appreciated the images of Japanese Apricot reflected on the water, and the poetically inspiring atmosphere where the trees are planted by the window. Fifth, the moon and snow were crucial weather conditions for appreciating. cold weather and time from night to dawn were ideally suited for enjoying. Sixth, they enjoyed blossoms in various fashions like bottling(甁梅), potting(盆梅), green-housing(龕梅), searching(龕梅) and black-and-white painting(墨梅) with a view to seeing blossoms earlier than the usual flowering time. Moreover, they used paper drapes, bead curtains, mirrors and ice lamps for active appreciation. They also listened to the sound of Piri(wind) and Geomungo(string), played go and drew tea with noble and elegant beauties when they enjoyed Japanese Apricot. The scholars influenced by the neo-Confucianism, which contemplates the objects, attached the specific sentiments like memories, grieves, dreams and farewells to Japanese Apricot and appreciated them. As stated above the scholars enjoyed the landscape including the picturesque scene like climate-weather, time-season and human behaviors not to mention the physical beauty of Japanese Apricot themselves and objects in company with Japanese Apricot including animals and plants.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.38 no.3
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• pp.11-24
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• 2020

The purpose of this study was to re-examine the neglected and forgotten Cheongdo Geoyeonjeong, to correct the wrong contents, examine the changes and conditions of the garden, and to establish basic data on the components of the forest in the future. In addition, it was extended to the Gongampungbyeog Cliff, the influence area of the Geoyeonjeongwonlim, and the results of the study were as follows; First, Based on the recitation of "Seonyu pungryu(仙遊風流)" in the "Cheongsuheon-yugo(聽水軒遺稿),", Dongchangcheon Stream and Gongampungbyeog(孔巖楓壁) were influenced by the outer gardens of the Georyeonjeongwonrim. Second, Small pavilion was built and arranged under the rock of Byeongam(Byeongpungbawi) in the management history of Geoyeonjeong Pavilion. The records show that Cheongsuheon used the Geoyeonjeong Pavilion as the original forest and even recognized Oewon, which is a scenic influence, as the Gongampungbyeog Cliff. Third, Many of the poems related to Gongam were recognized as Seunggyeong, which represents the Unmun area, and the eight scenery of Cheongdo and Unmungugok were established here as proof that Gongampungbyeog Cliff was very faithful to the traditional Seunggyeong aspect of Gongampungbyeog Cliff, and the crystalline structure of the location was implied as an external source of Geoyeonjeongwonlim. Fourth, The lower part of Dongchangcheon Stream, which stretches from Geoyeonjeongwonrim to Gongam, is filled with attractions consisting of cancerous areas such as Punghodae, Moseongam, Buangdae, Gokcheondae, Saganjeong, Hakgadae, and Hyeongjeam, which provide a clearer picture of the space and landscape of the Geyeonjeongwonrim Outer Garden. Fifth, The expression "dragging water, spilling it into the courtyard, and sending it back to the downtown of the field" of the Cheongsuheon-yugo suggests that the site of Geoyeonjeong Pavilion was originally a prevention. It is also inferred that Cheng Shu-heon also wanted to respect runners and pursue natural views like runners. Sixth, The record of planting a description of spring water and willow trees in "Geoyeonjeong Manyeong(居然亭晩影)」" and "Sanggukseol(霜菊說)」" suggests that the chrysanthemum was planted and planted, and that the chrysanthemum was used to describe the Osanggojeol(傲霜孤節), which means that he would not yield and keep his incision alone despite severe frost. Seventh, It is believed that the writing was written by Cheongsuheon in 1844 during the period of the creation of the Wonrim. The rock letters on the floor of Geoyeonjeong suggest the names of the receiving and the winning prizes. Most of the passages are based on nuclear power plants, including Muidogyo of the Zhuzi, and most of them incorporate the virtues of the Gunja and the natural views of the Eunja. In addition, the rock writing 'Gyeong(敬)' or 'Uidang(義堂)' is a substitute for special worship objects or introspection, adding to the significance and scenic properties of the Georyeon Garden Forest.

• Korean Journal of Breeding Science
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• v.41 no.3
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• pp.349-353
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• 2009

We developed a new barley cultivar "Dami" (Hordeum vulgare L.) with the auricleless gene lig (al, li, aur-a). The characteristic of auricleless is a spontaneous mutant type which has known as a monogenic recessive gene. The plant with the gene has erect leaf blades because of no auricle. The cultivar was derived from a cross between 'BGS60' and 'Kangbori'. 'BGS60' has the auricleless gene (li), while 'Kangbori' showed a high biomass with winter hardiness and resistance to BaYMV (Barley Yellow Mosaic Virus). Subsequent generations were handled by the bulk method in a pedigree selection program. A promising line showed both high yield and lodging resistance in the yield trials at Iksan in 2003 to 2004, and designated as Iksan414. The line was subsequently evaluated for winter hardiness, earliness, and yield in the seven locations around Korea for three years from 2005 to 2007 and was designated as "Dami" and released. It has the growth habit of III, erect plant type, green leaf and stem similar to the check cultivar 'Sunwoo' Its heading date was April 30, and maturing date May 31 in paddy field conditions, which were similar to those of 'Sunwoo' respectively. The cultivar Dami was 97 cm in culm length, had 643 spikes per $m^2$ and higher leaf dry weight, and better adaptability to dense planting, winter hardiness, and resistance to BaYMV than the check cultivar did. The average forage yield of "Dami" was about 12 ton $ha^{-1}$ in dry matter (33 ton $ha^{-1}$ in fresh matter) in paddy field. "Dami" also showed 7.5% of crude protein content, 28.5% of ADF (Acid Detergent Fiber), 50.1% of NDF (Neutral Detergent Fiber), and 66.4% of TDN (Total Digestible Nutrients), including higher grade of silage quality for whole crop barley. This cultivar would be suitable for the area where the daily minimum temperature of January is above $-8^{\circ}C$ in Korean peninsula.

• Korean Journal of Breeding Science
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• v.43 no.6
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• pp.611-615
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• 2011

'Manjong' is a new japonica rice cultivar developed in 2009 derived from a cross between 'Yeongdeog34' and 'Nampyeongbyeo' at the Department of Functional Crop, NICS, RDA. This cultivar is suitable for the double cropping system after the cash crop cultivation. Heading date of 'Manjong' is later than 'Keumobyeo' under the late transplanting cultivation on July 10. It has high grain fertility under cold conditions and low premature heading. One of the distinguishing characteristics of this variety is its resistance to major diseases like leaf blast and rice stripe virus disease. However, it shows susceptibility to major insect pests and bacterial blight disease. Milled rice kernels are translucent with non-glutinous endosperm and have 7.2% protein and 18.7% amylose contents. Ripening ratio and head rice ratio is very higher than those of 'Keumobyeo'. The palatability of cooked rice is also better than 'Keumobyeo'. The milled rice yield of 'Manjong' in local adaptability tests after harvest of the cash crop is $4.81MT\;ha^{-1}$. This cultivar is suitable for planting in the plain paddy fields of Honam and Yeonnam regions in Korea.

• Korean Journal of Breeding Science
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• v.41 no.2
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• pp.149-153
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• 2009

Junamjosaeng is a new japonica rice cultivar developed in 2006 from a cross between Milyang165*3 and Koshihikari at the Department of Functional Crop Science, NICS, RDA. This cultivar is suitable for the double cropping system (i. e., before and after the cash crop). Heading date of Junamjosaeng is 6 days earlier than Keumobyeo under the late transplanting cultivation on July 10. It has a high grain fertility under cold conditions and low premature heading. One of the distinguishing characteristics of this variety is its resistance to major diseases like leaf blast, bacterial blight races ($K_1$, $K_2$, $K_3$) and rice stripe virus disease. However, it showed susceptibility to major insect pests. Milled rice kernels are translucent with non glutinous endosperm and have 6.7% protein and 19.8% amylose contents. Milling recovery of head rice is 75.7%. The palatability of cooked rice is better than Keumobyeo. The milled rice yield of Junamjosaeng in local adaptability tests after harvest of the cash crop was $4.43\;tons\;ha^{-1}$. This cultivar is suitable for planting in the plain paddy fields of Honam and Yeonnam regions in Korea.

• Korean Journal of Heritage: History & Science
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• v.46 no.3
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• pp.114-133
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• 2013

This study aims to identify the natural damage of the Samcheonsaji Temple Site in Bukhansan National Park and to suggest the plans to minimize damage for the remains. The types of natural damage are classified into direct vegetation damage, indirect topographical damage, and artificial damage. The most popular causes of damage to temple sites include the roots of trees as direct vegetation damage and the soil erosion by rain or stream as topographical damage. Direct vegetation damage includes burial remains damaged by the root of trees and vines, but it is often observed that some trees have contributed to protection against collapse. Indirect topographical changes have damaged the ruins by soil erosion caused by floods or typhoons. Vegetation changes due to topographical reasons have also caused damage. Artificial reasons of damage include forestry operations and compaction by hikers. Based on the analysis of the findings, the following could be suggested as plans to resolve these problems: 1. Natural damage occurs slowly due to negligence. Therefore, it could be reduced by forestry improvement, including forest density control through thinning, planting to prevent landslides, maintaining grasslands nearby. 2. Deciduous broadleaf trees can be planted to reduce soil erosion by rainfall. It is necessary to maintain the density of forests at around $0.02{\sim}0.18trees/m^2$. 3. It would be good to grow Quercus spp and Carpinus spp or weaken the community of Robinia pseudoacacia and Pueraria lobata which disturb the ecosystem. Samcheonsaji Temple Site is located in Mt. Bukhan National Park that is a publicly owned property. Therefore, it is constantly maintained for natural preservation and vegetation management could be considered for the preservation of historical remains.