• Mycobiology
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• v.51 no.5
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• pp.360-371
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• 2023

Hispidin is an important styrylpyrone produced by Sanghuangporus sanghuang. To analyze hispidin biosynthesis in S. sanghuang, the transcriptomes of hispidin-producing and non-producing S. sanghuang were determined by Illumina sequencing. Five PKSs were identified using genome annotation. Comparative analysis with the reference transcriptome showed that two PKSs (ShPKS3 and ShPKS4) had low expression levels in four types of media. The gene expression pattern of only ShPKS1 was consistent with the yield variation of hispidin. The combined analyses of gene expression with qPCR and hispidin detection by liquid chromatography-mass spectrometry coupled with ion-trap and time-of-flight technologies (LCMS-IT-TOF) showed that ShPKS1 was involved in hispidin biosynthesis in S. sanghuang. ShPKS1 is a partially reducing PKS gene with extra AMP and ACP domains before the KS domain. The domain architecture of ShPKS1 was AMP-ACP-KS-AT-DH-KR-ACP-ACP. Phylogenetic analysis shows that ShPKS1 and other PKS genes from Hymenochaetaceae form a unique monophyletic clade closely related to the clade containing Agaricales hispidin synthase. Taken together, our data indicate that ShPKS1 is a novel PKS of S. sanghuang involved in hispidin biosynthesis.

• Journal of ILASS-Korea
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• v.4 no.3
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• pp.42-52
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• 1999

An effectiveness of thermal utilization of a dousing system in the 600 MW PHWR Nuclear Power Plant has been evaluated. The behavior and conditions of water droplet sprayed in a postulated accident conditions in containment configuration has been calculated. In this calculation, two pressure conditions with the consideration of obstruction area and containment wall effect has been established : one being the minimum containment pressure of 7 kPa(g) encountered for dousing shut off and the other being the containment design pressure 124 kPa(g). The results revealed that the effectiveness of the thermal utilization ranges from 93% to 97%. In the analysis on two cases without/with side wall effect in the containment building, the thermal utilization decreases with obstruction area from 89% to 85%, which satisfies the design criteria set for the containment pressure against the accident condition.

• Korean Journal of Plant Resources
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• v.10 no.2
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• pp.200-210
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• 1997

The understanding on the plant genome is accelerated with the fast advance of molecular biological techniques. The molecular dissecting of the plant genome has made possible the precise genotyping the plants, which can be utilized for molecular breeding program. As well, the molecular cloning of genes interested can facilitate the process of gene transfer between intra-and inter-generic taxa. Moreover, the manipulation of the agronomically important QTL genes, which can be rarely performed by the conventional genetic methods, is also possible by the utilization of molecular markers. In addition to these genetical applications, molecular markers are useful in the areas of plant taxonomy and management of germplasm by fingerprinting analysis. This paper describes the theoretical aspects marker technologies and practical applications of each marker technique.

• Proceedings of the Plant Resources Society of Korea Conference
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• 1995.01a
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• pp.27-27
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• 1995

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2004.10a
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• pp.127-127
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• 2004

• The Plant Pathology Journal
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• v.17 no.3
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• pp.123-127
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• 2001

No Abstract.See Full-text

• Journal of Power System Engineering
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• v.8 no.1
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• pp.48-54
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• 2004

In this study, waste heat of Jeju City Waste Environment Center is investigated and the utilization method is suggested with economical analysis of additional investment that needed for new facility. Energy balance of the typical facilities is considered in this study such as incineration plant and LFG power plant. The payback period of the investment which is used for the LFG power plant waste heat utilization facility is about 2.4 years and the economic profit of the facility during 10 years operation is up to 926 million won.

• Proceedings of the Korean Institute of Communication Sciences Conference
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• 1987.04a
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• pp.82-85
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• 1987

This paper describes the technology for the improvement of subxdriber loop utilization. Nearly all customer loops are now carried at voice frequency on individual wire pairs. Reenct digital technology improvements together with the increasing cost of wire, have resulted in some penetration of carrier systems into the loop plant. Moreover the increasing use of local digital switching systems should permit digital carrier systems to become an important factor in the loop plant in the future.

• Journal of Microbiology and Biotechnology
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• v.29 no.10
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• pp.1624-1628
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• 2019

Banana planting altered microbial communities and induced the enrichment of Fusarium oxysporum in rhizosphere compared with that of forest soil. Diseased plant rhizosphere soil (WR) harbored increased pathogen abundance and showed distinct microbial structures from healthy plant rhizosphere soil (HR). The enriched taxon of Bordetella and key taxon of Chaetomium together with some other taxa showed negative associations with pathogen in HR, indicating their importance in pathogen inhibition. Furthermore, a more stable microbiota was observed in HR than in WR. Taken together, the lower pathogen abundance, specific beneficial microbial taxa and stable microbiota contributed to disease suppression.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2005.11a
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• pp.29-47
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• 2005