• Title/Summary/Keyword: Plant stimulation

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Anti-inflammatory Effects of Scrophularia Koraiensis Nakai via NF-κB and MAPK Signaling Pathways in LPS-induced Macrophages

  • Da-Yoon Lee;So-Yeon Han;Hye-Jeong Park;Seo-Yoon Park;Jun-Hwan Jeong;Yoon-Jae Kwon;Tae-Won Jang;Jae-Ho Park
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2022.09a
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    • pp.107-107
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    • 2022
  • Scrophularia koraiensis Nakai is widely used to remedy fever, edema, and neuritis. S. koraiensis has harpagoside and angoroside C, these compounds have been reported to alleviate inflammation, rheumatic diseases, and analgesic stimulation. We evaluated the anti-inflammatory effects of the ethanol extract of S. koraiensis (SKE) in lipopolysaccharides (LPS)-induced macrophages. At cellular levels, SKE decreased the production of nitric oxide (NO), the expression of inducible nitric oxide synthase (iNOS), and cytokines (IL-1b, TNF-a, and IL-6) under the LPS stimulation. SKE inhibited the phosphorylation of nuclear transcription factor-kappa B (NF-κB) p65 and its inhibitor (IκB-α). In addition, SKE suppressed the phosphorylation of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 in the mitogen-activated protein kinase (MAPK) pathway. In conclusion, SKE could be considered a potential resource for attenuating inflammation response and it may be utilized in the material for cosmetics, food additives, and tea.

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STUDIES ON THE PHYSIOLOGY OF DEVELOPMENT IN CROPS. 4. STUDIES ON PHYTOPERIODICAL CONTROL FOR TUBER FORMATION IN SWEET POTATO

  • Kim, Yong-Choll
    • Journal of Plant Biology
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    • v.2 no.1
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    • pp.35-42
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    • 1959
  • The cuttings and apical portion of stein in sweet potato were growlh under artificial light and specific photoperied and temperature. Though the plant growth was poor under insufficient light intensity of artificial light, the tuber formations were induced at long light period (16L+8D) and not induced at short light period (8L+16D) and low temperature of darkparied. The determinative factor for tuber formation of sweet potato seems to be a stimulation which has intimate relationship with specific photoperiod and temperature and no direct relation with the growth of plant body and light intensity. The root pattern of inductive state for tubers and non-inductive state were different distinctly, the former were silky and slender, the latter were branchy, and stout appearence. This different root pattern must be due also to the specific photoperiod and temperature and may have any relationship with the stimulation for tuber formation from the point of auxin physiology etc.

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The Stimulation of Arginine Decarboxylase Activity by alpha-Difluoromethyl$ Ornithine in Tobacco Suspension Cultured Cells

  • Lee, Sun-Hi;Kim, Yong-Bum;Lee, Myeong-Min;Park, Ki-Young
    • Journal of Plant Biology
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    • v.39 no.2
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    • pp.107-112
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    • 1996
  • To study the compensatory aspect of putrescine biosynthetic enzyme n tobacco suspension cultured cells, we examined the contents of the cellular polyamines and the activities of arginine decarboxylase (ADC, EC 4.1.1.19) and ornithine decarboxylase (ODC, EC 4.1.1.17) in the tobacco suspension cells treated with $\alpha$-difluoromethyl arginine (DFMA) or $\alpha$-difluoromethyl ornithine (DFMO). In the untreated cells, the content of the cellular putrescine was decreased during the first 3 hours and then subsequently increased. However, the content of the cellular spermidine and spermine remained constant during the incubation time. While ADC activity increased after 6 hours, ODC activity decreased following the rapid increase until 6 hours. DFMA induced the decrease in the contents of putrescine and spermidine, and the increase in that of spermine. It also caused the inhibition of ADC and ODC activities throughout the incubation time. DFMO produced the stimulation of ADC activity about 2 times of untreated cells and the decrease in the content of putrescine about 50% of them at 12 hour. The application of putrescine or cycloheximide prevented the increase of ADC activity by DFMO but that of actinomycin-D did not show any detectable effect. The stimulation of ADC activity by DFMO in tobacco suspension cultured cells was probably due to the enhancement of de novo synthesis for ADC protein, which might be regulated in the translation step by the content of the cellular putrescine.

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Pharmacological Studies on Pseudostellaria palibiniana ("큰개별꽃" 엑기스의 약리학적(藥理學的) 연구(硏究))

  • Yang, Ki-Sook;Kim, Tae-Hee
    • Korean Journal of Pharmacognosy
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    • v.15 no.1
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    • pp.6-14
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    • 1984
  • Pharmacological studies have been carried out with the methanol extract of the whole plant of Pseudostellaria palibiniana Ohwi (Caryophyllaceae). The results showed that it had stimulation effect on heart, hypotensive actions depending on extract contents, stimulation effect on respiration, contraction on excised intestines, enhancement of tension on excised uterus, antineoplastic activity on Ehrlich carcinoma, liver protective activity against $CCl_4$ intoxication, writhing and diuretic activities.

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Stimulation Effect of a Soft Ferrite Ceramic Powder on Growth in Plant Cell and Tissue Cultures (연자성 세라믹 분말에 의한 식물세포 및 조직의 생장촉진 효과)

  • Ahn, Jun-Cheul;Kim, Yu-Jung;Park, Chan-Young;Hwang, Baik
    • KSBB Journal
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    • v.13 no.5
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    • pp.530-534
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    • 1998
  • The addition of the ceramic powder as state of bare in culture medium has stimulated the growth of Achyranthes japonica in both the disorganized cell and the plantlet. The grwoth rate of Hyoscyamus niger adventitious root and Pylatycodon grandiflorum hairy root was enhanced up to 100 and 250%, respectively, even though Scopolia parviflora hairy root and Hyoscyamus albus adventitious root were not. The ceramic powder has enhanced the growth of H. niger adventitious root even in a test tube immersed into its culture medium to irradiate alone without any direct contact. The ceramic powder seems to have a significant role on both the growth and the physiological action of some plants.

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Changes in Shoot and Root Growth of Tomato Seedlings Stimulated by Brushing (브러싱 자극 토마토 공정묘의 지상부와 지하부 생육 변화)

  • Hyeon Woo Jeong;Hee Sung Hwang;Seung Jae Hwang
    • Journal of Bio-Environment Control
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    • v.32 no.3
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    • pp.205-209
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    • 2023
  • Mechanical stimulation induce the morphological changes in plants. In this study, we investigated the growth changes of tomato seedlings applicated to mechanical stimulation. The brushing treatment was used for mechanical stimulation. The brushing treatment interval was 2 hr using transfer device attached acrylic film from 10 days after sowing. Growth parameter of tomato seedlings were measured 3-day intervals to investigate the growth changes during brushing treatment. The plant height and leaf area were decreased in brushing treatment than the control, and the fresh and dry weights of shoot didn't have significant difference in the control and brushing treatment. The total root length and root surface area were increased in brushing treatment compared than the control, and root volume has no significant difference in the control and brushing treatment. In conclusion, these results suggest that the application of brushing treatment on tomato seedling make shorten plant height and well-development root morphological characteristics.

Growth Suppression of Tomato Plug Seedlings as Affected by Material Type for Brushing Stimulation (브러싱 소재에 따른 토마토 공정묘의 생장억제)

  • Jeong, Hyeon Woo;Lee, Hye Ri;Hwang, Hee Sung;Kim, Eun Bin;Hwang, Seung Jae
    • Journal of Bio-Environment Control
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    • v.29 no.4
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    • pp.313-319
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    • 2020
  • This study was conducted to find proper material of brushing stimulation for height suppression of tomato plug seedlings. The tomato seeds were sown in 40-cell plug tray filled with commercial seedling medium and brushing stimulation was started at 18 days after sowing. Acrylic, polypropylene film, and weaving film were used by materials of brushing stimulation and, non-treatment and diniconazole treatment were used as the control. In acrylic treatment, the plant height was the shortest and the stem diameter was the thickest. Leaf growth was the lowest in diniconazole treatment. However, the SPAD value was the greatest in diniconazole treatment. The dwarf rate was the greatest in acrylic treatment. In acrylic treatment, the T/R ratio was the lowest and compactness was the greatest. In conclusion, use of the acrylic as material for brushing stimulation has higher dwarf rate than diniconazole treatment, and has advantages height suppression and seedling quality.

Development of transgenic sweet potato producing human lactoferrin (인체 락토페린 생산 형질전환 고구마 개발)

  • Min, Sung-Ran;Kim, Jae-Wha;Jeong, Won-Joong;Lee, Young-Bok;Liu, Jang R.
    • Journal of Plant Biotechnology
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    • v.36 no.3
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    • pp.224-229
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    • 2009
  • Human lactoferrin is an iron-binding glycoprotein with many biological activities, including the protection against microbial and virus infection and stimulation of the immune system. We introduced a human lactoferrin (hLf) cDNA under the control of 35S promoter into sweet potato by particle bombardment. Transgenic plants were regenerated via somatic embryogenesis. Transgenic plants were produced typical tuberous roots in soil. PCR, Southern and northern analyses confirmed that the hLf cDNA was incorporated into the plant genome and was properly expressed in plants. Western blot analysis showed that the 80 kDa full length hLf protein was produced in transgenic tuberous roots. Overall results indicated that sweet potato would be an excellent host to produce human therapeutic proteins.