• Title/Summary/Keyword: Plant specimens

Search Result 456, Processing Time 0.033 seconds

Methanol fixation for scanning electron microscopy of plants

  • Ki Woo Kim
    • Applied Microscopy
    • /
    • v.50
    • /
    • pp.10.1-10.6
    • /
    • 2020
  • Plant specimens for scanning electron microscopy (SEM) are commonly treated using standard protocols. Conventional fixatives consist of toxic chemicals such as glutaraldehyde, paraformaldehyde, and osmium tetroxide. In 1996, methanol fixation was reported as a rapid alternative to the standard protocols. If specimens are immersed in methanol for 30 s or longer and critical-point dried, they appear to be comparable in preservation quality to those treated with the chemical fixatives. A modified version that consists of methanol fixation and ethanol dehydration was effective at preserving the tissue morphology and dimensions. These solvent-based fixation and dehydration protocols are regarded as rapid and simple alternatives to standard protocols for SEM of plants.

Mechanical properties and adiabatic temperature rise of low heat concrete using ternary blended cement

  • Kim, Si-Jun;Yang, Keun-Hyeok;Lee, Kyung-Ho;Yi, Seong-Tae
    • Computers and Concrete
    • /
    • v.17 no.2
    • /
    • pp.271-280
    • /
    • 2016
  • This study examined the mechanical properties and adiabatic temperature rise of low-heat concrete developed based on ternary blended cement using ASTM type IV (LHC) cement, ground fly ash (GFA) and limestone powder (LSP). To enhance reactivity of fly ash, especially at an early age, the grassy membrane was scratched through the additional vibrator milling process. The targeted 28-day strength of concrete was selected to be 42 MPa for application to high-strength mass concrete including nuclear plant structures. The concrete mixes prepared were cured under the isothermal conditions of $5^{\circ}C$, $20^{\circ}C$, and $40^{\circ}C$. Most concrete specimens gained a relatively high strength exceeding 10 MPa at an early age, achieving the targeted 28-day strength. All concrete specimens had higher moduli of elasticity and rupture than the predictions using ACI 318-11 equations, regardless of the curing temperature. The peak temperature rise and the ascending rate of the adiabatic temperature curve measured from the prepared concrete mixes were lower by 12% and 32%, respectively, in average than those of the control specimen made using 80% ordinary Portland cement and 20% conventional fly ash.

Evaluation of Degraded Materials by Automated Ball Indentation(ABI) Technique (압입법을 이용한 재료의 열화도 평가)

  • 김석민;석창성;이승석;권재도
    • Journal of the Korean Society of Safety
    • /
    • v.15 no.1
    • /
    • pp.28-35
    • /
    • 2000
  • As huge energy transfer systems like a nuclear power plant, steam power plant and petrochemical plant are operated for a long time, mechanical properties are changed by degradation. The life time of the systems can be affected by the mechanical properties. So determination of the integrity of the metallic structure is required either to en4sure that failure will not occur during the service life of the components or to evaluate the lifetime extension of the structure. An automated ball indentation(ABI) method was developed as a non-destructive technique for evaluating the integrity of such metallic components. In this paper, we would like to present the aging evaluation technique by the ABI method. The five classes of the thermally aged CF8M specimens were prepared using an artificially accelerated aging method. After holding 100, 300, 900, 1800 and 3600 hours at 43$0^{\circ}C$, the specimens were cooled down using water to room temperature respectively. The tensile test, hardness test, charpy impact test, ABI test were performed. The results of the fracture test were compared with those of ABI test and the new evaluation technique of the integrity of metallic structures was developed.

  • PDF

Creep Damage Evaluation of High-Temperature Pipeline Material for Fossil Power Plant by Ultrasonic Test Method (초음파에 의한 발전소 고온배관재료의 크리프손상 평가)

  • Lee, Sang-Guk;Chung, Min-Hwa
    • Journal of Ocean Engineering and Technology
    • /
    • v.13 no.2 s.32
    • /
    • pp.99-107
    • /
    • 1999
  • Boiler high-temperature pipelines such as main steam pipe, header and steam drum in fossil power plants are degraded by creep damage due to severe operationg conditions which are high temperature and high pressure for an extended period time. Such material degradation leads to various component failures causing serious accidents at the plants. Conventional measurement techniques such as replica method, electric resistance method, and hardness test method have such disadvantages as complex preparation and measurement procedures, too many control parameters, and therefore, low practicality and they were applied only to component surfaces with good accessibility. In this paper, artificial creep degradation test and ultrasonic measurement for their creep degraded specimens have been carried out for the purpose of evaluation for creep damage which can occur in high-temperature pipeline of fossil power plant. Absolute measuring method of quantitative ultrasonic measurement for material degradation was established, and long term creep degradationtests using life prediction formula were carried out. As a result of ultrasonic tests for crept specimens, we confirmed that the sound velocity decreased and the attenuation coefficient linearly increased in proportion to the increase of creep fractiin(${\phi}$c).

  • PDF

Actual distribution and present status of a threatened aquatic plant, Sagittaria aginashi (Alismataceae), in Korea

  • Shiga, Takashi;Tsubota, Kazumasa;Choi, Hyeok Jae
    • Korean Journal of Plant Taxonomy
    • /
    • v.47 no.3
    • /
    • pp.180-188
    • /
    • 2017
  • We evaluated the actual distribution, present status, and number of remnant individuals of Korean Sagittaria aginashi (Alismataceae) based on herbarium specimens and field surveys. We also reidentified Korean S. aginashi by analyzing sequences of the internal transcribed spacer (ITS) region. We found nine specimens of S. aginashi in herbaria from four localities housed at the Osaka Museum of Natural History (OSA) and at the Korea National Herbarium (KH). During our field research, we could not confirm the current statuses of two collection localities (Taereung and Ansan-si), though this was not the case for Ulsan-si. In addition, we found two new localities in Ulsan-si and Yangsan-si. These three remnant populations are distributed in wetlands in mountainous areas (elev. 480-740 m). With regard to the number of flowering and immature individuals in the three localities, there were <50 and <2,000, respectively, in total. ITS sequences demonstrated that the sequences of Korean and Japanese S. aginashi are uniform and distinct from other Sagittaria species. These results indicate that S. aginashi is a rare and threatened species in Korea. It should be listed as an endangered species on the Korean Red List and requires urgent protection by conservation programs, including the extensive surveys of other possible natural habitats.

Load transfer test of circular anchorage system according to ETAG 013 (ETAG 013 규정에 따른 원형 정착구의 하중전달실험)

  • Kim, Bum-Joon;Kim, Hyun-Gi
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.19 no.5
    • /
    • pp.167-175
    • /
    • 2018
  • Load transfer experiments have been carried out to evaluate the performance of the anchorage and it has been stated that the specimens were manufactured and tested according to ETAG 013. On the other hand, the amount of spiral reinforcement and auxiliary reinforcement exceeded the amount specified in ETAG 013. In this study, the load transfer test method and the criteria specified in ETAG 013 were considered and the PT anchorage system was applied to manufacture specimens with high strength concrete and a high tensile prestressing strand. A load transfer test according to ETAG 013 was performed to evaluate the performance of the circular anchorage. As a result, it was confirmed that ETAG 013 is a very strict specification that does not satisfy the performance of an anchorage unless the specimens of an appropriate size and spiral reinforcement are used. To assess the stability of the specimens, increasing the size of the specimen by 15%, rather than increasing the amount of auxiliary reinforcement, is considered to be the correct method in accordance with ETAG 013.

Plant-parasitic Nematodes Associated with Commercial Orchards of Passion Fruit and Adjacent Cerrado Vegetation in the Brazilian Federal District

  • Castro, Ana Paula Gomes De;Goulart, Alexandre Moura Cintra;Andrade, Ednalva Patricia De;Cares, Juvenil Enrique;Carvalho, Daniel Diego Costa
    • The Plant Pathology Journal
    • /
    • v.28 no.3
    • /
    • pp.306-310
    • /
    • 2012
  • Populations of plant-parasitic nematodes were evaluated in soil and root samples from areas planted with passion fruit, or covered with adjacent native cerrado vegetation, in five different cropping regions of the Brazilian Federal District. From July 2007 to January 2008, twenty samples had been collected in five Rural Centers (N$\acute{u}$cleo Rural-NR) planted with passion fruit. In five sites, samples were also collected in native cerrado vegetation. Specimens of nine genera of plant-parasitic nematodes (Meloidogyne, Helicotylenchus, Rotylenchulus, Scutellonema, Pratylenchus, Paratylenchus, Hemicycliophora, Xiphinema and Criconemoides) were found in the present survey. Important nematode pathogens, such as Rotylenchulus reniformis and Meloidogyne spp. were observed in the passion fruit plantations throughout five major producing regions of the Brazilian Federal District.

Morphological characteristics, distribution and taxonomic consideration of Chionanthus retusus Lindl. & Paxton in Korea (한국산 이팝나무(Chionanthus retusus Lindl. & Paxton)의 형태학적 특징, 분포 및 분류학적 검토)

  • Song, Jun-Ho;Kong, Min-Jung;Hong, Suk-Pyo
    • Korean Journal of Plant Taxonomy
    • /
    • v.41 no.2
    • /
    • pp.156-163
    • /
    • 2011
  • The morphological characteristics of Chionanthus retusus Lindl. & Paxton in Korea are reviewed in this study through the comparison with its previous records and a distribution map of this species is created using previous flora and voucher specimens. The leaf epidermal micromorphology and the petiole anatomy are also briefly described.