• 원예과학기술지
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• 제28권4호
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• pp.535-539
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• 2010

국내 육성 품종인 '매향' 딸기의 육묘 기간 중 광합성유효광량자속의 환경 조절을 통하여 러너 및 러너플랜트의 발생과 생산 효율을 증대시키고자 하였다. 딸기 육묘에 이용된 증식체의 엽수와 관부 직경은 각각 $3.1{\pm}0.4$와 $7.0{\pm}1.1mm$였다. 광합성유효광량자속을 각각 140, 210 및 $280{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$로 설정한 9기의 인공광 이용형 육묘 모듈에서 35일 동안 육묘하였다. 러너플랜트 생산 효율은 증식체당 일일 0.27주로 처리구 중 유의하게 가장 높았으며 관행의 딸기 육묘 방식의 러너플랜트 생산 효율과 비교하여도 크게 증진되었음을 확인하였다. 폐쇄형 육묘 시스템을 활용하여 육묘 기간 중의 PPF를 $280{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$로 조절하면 국내 육성 품종인 '매향'의 러너 발생 및 러너플랜트 생산을 증진시킬 수 있음이 확인되었다. 따라서 최근 국내에서 육성된 신품종 딸기의 급속 보급을 위한 증식체계 구축에 본 연구 기술이 활용되면 육성된 품종의 조급 보급이 가능할 것으로 판단된다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.15-15
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• 2019

The walnut (Juglans regia L.), a member of the Juglandaceae, is native to the mountain ranges of central Asia. This species of walnut is valued commercially for its nuts and in some areas for its timber. The seeds of walnut are recalcitrant and it has strong integument dormancy and their germination is irregular, making its natural propagation difficult. Low percentage of seed germination and long propagation cycle are the main problems of propagation. This study was conducted medium composition on in vitro plantlet regeneration from axillary buds of walnut. It has proved to be the most generally applicable and reliable method of in vitro propagation. Micropropagation culture that axillary buds are excised aseptically enables faster multiplication of plants. The axillary buds of walnut new cultivar "Sinlyeong" were cultured on two basal media which contained the different plant growth regulators depending on the respective shooting and rooting stage. After 12 weeks, the shoot generation rate was 85.3%, the shoot number and its length were 1.9/explant and 2.7 cm in the most favorable medium composition. The percentage of rooting was 25.4%. From these results, it was found the optimum basal medium and plant growth regulator for in vitro plant regeneration from axillary buds of walnut new cultivar "Sinlyeong". However, we have continued to search the other medium additives to enhance the rate of walnut root.

• Journal of Plant Biology
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• 제31권4호
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• pp.249-258
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• 1988

Changes in starch content and activities of ADPG- and UDPG-starch synthase and $\alpha$- and, $\beta$-amylase were studied in order to investigate effects of gibberellic aicd and abscisic acid on endogenous starch content during shoot differentiation and protocorm propagation in Cymbidium spp. (Jungfrau) protocorm. Shoot differentiation was promoted during the degradation of endogenous starch and protocorn propagation was promoted during starch accumulation in protocorm. The activities of ADPG- and, UDPG-starch synthase and $\alpha$- and $\beta$-amylase seemed to be related with starch content. Shoot differentiation and protocorm propagation were slightly inhibited in protocorm explants treated with 100$\mu$M gibberellic acid. The explants treated with 10$\mu$M abscisic acid lost the capacity for shoot differentiation and protocorm propagation, and that could not be overcome by 100$\mu$M gibberellic acid added to culture medium. Starch content fluctuated as the control even after 10$\mu$M abscisic acid. None the less, the treatment completely inhibited shoot differentiation and protocorm propagation.

• 한국토양비료학회지
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• 제49권5호
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• pp.608-613
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• 2016

Arbuscular mycorrhizal fungi (AMF) spore propagation and long term maintenance is still a complicated technique for farmers. The use of AMF for their ability to promote plant growth and protect plants against pathogen attack and environmental stresses demands AMF propagation for large scale application. This study aimed to propagate AMF spores by trap culture technique and assess their ability to propagate with different host plants in a continuous plant cycle. Mycorrhizal inoculation by trap culture in maize resulted in longer shoots and roots than sudangrass plants. Increase in dry weight with higher percentage also was observed for maize plants. After first and second plant cycle, maize plants had the higher percentage of mycorrhizal response in terms of colonization and arbuscules than sudangrass. Maximum in spore count also achieved in the pots of maize plants. The results show that maize plant is more suitable host plant for AMF spore propagation and trap culture technique can be used effectively to maintain the AMF culture for long time.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 추계학술대회
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• pp.1721-1726
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• 2004

Heat rate is a representative index to estimate the performance of turbine cycle in nuclear power plant. Accuracy of heat rate calculation is dependent on the accuracy of measurement for plant status variables. Uncertainty of heat rate can be modeled using uncertainty propagation model. We developed practical estimation model of heat rate uncertainty using the propagation and regression model. The uncertainty model is used in the performance analysis system developed for the operating nuclear power plant.

• 한국자원식물학회지
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• 제15권1호
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• pp.8-17
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• 2002

새 번식방법 개발을 위해 둥굴레 뿌리는 눈이 없어도 부정아가 발생된다는 점에 착안하여 뿌리길이와 굵기에 대한 부정아 발생에 관한 시험 연구를 한 결과는 다음과 같다. 1년차는 뿌리길이가 5cm 경우 66.5%지만 15cm에서는 113% 부정아 발생되었고, 뿌리의 굵기 5mm이하는 98.3%지만 10mm이상은 113.3%로 46.7% 차이를 보였다. 2년차는 부정아 생성률은 15cm에서는 1년차의 3배, 시험구 설치 당시 전혀 눈이 없는 상태에서 4배의 증식을 보였다. 용토는 일반토양 2.6배 보다 모래나 펄라이트가 3.1~3.3배 증식 되었다. 대량번식을 위한 가장 좋은 방법은 10mm 둥굴레 뿌리를 5cm로 절단하여 모래에 식재하는 방법으로 판단된다.

• Plant Resources
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• 제3권3호
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• pp.219-222
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• 2000

For the purpose of establishing an efficient propagation through airlift bioreactor system of Zingiber of officinale Rosc. Korean native Seosanjong, the effect of different factors and bioreactor on cultured plantlets were investigated. The highest number of plantlets, fresh weight per plant was obtained from explants when cultured in MS liquid medium including 0.3 mg/L NAA and 2.0 mg/L kinetin for 40 days. A 10 L bottle type bubble bioreactor, compared with 250 mL Erlenmeyer flask, was more efficient, producing 4.7 plantlets or from 1.5 to 1.6 times more than did the Erlenmeyer flask. The results demonstrate the rapid mass propagation of airlift bioreactor to produce normal ginger.

• Journal of Forest and Environmental Science
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• 제37권4호
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• pp.304-308
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• 2021

Iris koreana (Iridaceae) is an endangered plant native to Korea. In order to develop an in vitro propagation method, we investigated the effect of 2,4-dichlorophenoxy acetic acid (2,4-D) and a-naphthalene acetic acid (NAA) on callus induction in different I. koreana tissues. In addition, we also investigated the effect of 2,4-D and Benzyl aminopurine (BA) treatments on adventitious shoot induction in viable calli and the effect of indole-3-butyric acid (IBA) on root formation in viable shoots. We found that callus production was highest with 1.0 mg/L NAA (94.4% cultured rhizome explants), and adding low concentrations of 2,4-D to BA containing media significantly increased the frequency of shoot primordial formation. The best rooting results were obtained with 1.0 mg/L IBA, on which 98% of regenerated shoots developed roots and produced an average of 7.4 roots within 45 days. This in vitro propagation protocol will be useful for conservation, as well as for mass propagation.

• Plant Biotechnology Reports
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• 제3권1호
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• pp.1-56
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• 2009

Orchid seeds are nearly microscopic in size. Because of that, many fanciful theories were proposed for the origin of orchids. Almost 400 years separate the time when orchid seeds were seen for the first time and the development of a practical asymbiotic method for their germination. The seeds were first observed and drawn during the sixteenth century. Seedlings were first described and illustrated in 1804. The association between orchid and fungi was observed as early as 1824, while the requirement for mycorrhiza for seed germination was established in 1899. An asymbiotic method for orchid seed germination was developed in 1921. After Knudson's media B and C were formulated, orchids growing and hybridization became widespread. Hybrids which early growers may not have even imagined became possible.

• 한국자원식물학회지
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• 제14권1호
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• pp.53-59
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• 2001

본 연구는 음나무의 대량증식을 위한 실용적인 실생번식과 녹지 삽목 기술을 개발하고자 실시 하였다. 실생 번식을 위한 노천매장과 1$0^{\circ}C$ 후숙처리는 1주이내에 배의 크기를 3mm이상 유도하였고 발아율은 대조구에 비해 노천매장과 1$0^{\circ}C$에서 후숙된 종자에서 70%이상 높게 나타났다. 녹지 삽목 실험에서 는 삽수의 모수령이 발근에 미치는 효과를 조사한 결과를 보면, 1년생 삽목묘 신초를 삽수로 사용한 것이 68.9%의 발근율로 가장 높고, 모수의 수령이 유령일수록 발근율이 높고, 반면 수령이 증가할수록 발근 능력이 감소하였다. 삽목 시기별 발근 실험에 있어서 1차 생장한 6월 13일 삽목한 삽수가 68.9%로 발근율이 가장 높아 6월중순이 삽목 적기였다. 식물생장조정제 종류 및 농도별 처리에서는 IBA 1000mg.L$^{-1}$ 농도에서 30분간 침적했을 때 발근율이 70%, NAA 1000mg.L$^{-1}$에서 66.7%의 순으로 2000mg.L$^{-1}$ 30초, 500mg.L$^{-1}$ 10시간 처리구 보다 발근율이 높게 나타났다.