• Genomics & Informatics
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• v.19 no.2
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• pp.19.1-19.9
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• 2021

Plant height is an important component of plant architecture and significantly affects crop breeding practices and yield. We studied DNA variations derived from F5 recombinant inbred lines (RILs) with 96.8% homozygous genotypes. Here, we report DNA variations between the normal and dwarf members of four lines harvested from a single seed parent in an F6 RIL population derived from a cross between Glycine max var. Peking and Glycine soja IT182936. Whole genome sequencing was carried out, and the DNA variations in the whole genome were compared between the normal and dwarf samples. We found a large number of DNA variations in both the dwarf and semi-dwarf lines, with one single nucleotide polymorphism (SNP) per at least 3.68 kb in the dwarf lines and 1 SNP per 11.13 kb of the whole genome. This value is 2.18 times higher than the expected DNA variation in the F6 population. A total of 186 SNPs and 241 SNPs were discovered in the coding regions of the dwarf lines 1282 and 1303, respectively, and we discovered 33 homogeneous nonsynonymous SNPs that occurred at the same loci in each set of dwarf and normal soybean. Of them, five SNPs were in the same positions between lines 1282 and 1303. Our results provide important information for improving our understanding of the genetics of soybean plant height and crop breeding. These polymorphisms could be useful genetic resources for plant breeders, geneticists, and biologists for future molecular biology and breeding projects.

• Journal of Plant Biology
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• v.22 no.3
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• pp.63-69
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• 1979

The plant growth and net production, the nitrogen uptake and recycling, the nitrogen mobility and allocation to each organ, and the nitrogen utility from the Italian rye grass field during the population development were analyzed in comparison with different clipping treatments. The maximum dry matter standing crop and nitrogen quantity of harvest increased significantly, however, the annual amounts of dry matter and nitrogen assimilation showed little variations with increasing clipping frequencies. Plants treated with frequent clippings allocated relatively more nitrogen to leaves and less to roots during the experimental period. The amount of recycling of nitrogen decreased considerably due to frequent clippings. The annual averages of nitrogen utility indices changed in inverse relation to the nitrogen availability; such as 63, 58, 44 and 35 for C, A, M and J plots, respectively.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 1995.06b
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• pp.21-25
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• 1995

;Bacterial blight caused by Xanthomonas oryzae pv. Olyzae is one of the most important diseases of rice. Host resistance, which relies on single, dominant resistance genes, is the only reliable method to control the disease at present. Pathogenic variation of the bacteria has been shown to follow the deployment of resistance genes in commercial cultivars. Information on the factors and the mechanisms for genetic variation of this pathogen is limited. Further, we have no clear evidence of whether population variability is due to sexual recombination or to variation introduced by mutations or intragenic recombination in a clonally maintained population.(omitted)itted)

• Korean Journal of Plant Taxonomy
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• v.52 no.4
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• pp.251-254
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• 2022

Saxifraga cortusifolia Siebold & Zucc. (Saxifragaceae, sect. Irregulares, ser. Rufescentes), a species considered endemic along mountain streams throughout Honshu, Shikoku, and Kyushu in Japan, was discovered as a wild population in the Cheona Valley in Haean-dong, Jeju-si on Jeju-do Island of Korea. The population consisted of three subpopulations occupying narrow areas 10 × 10 m², 5 × 5 m², and 10 × 5 m² in size. The number of individuals in the three subpopulations was confirmed to exceed 100. Most of the individuals were on or below rocks at the edge of the valley. A morphological description, detailed photos, and a key to related taxa in Korea are presented.

• The Plant Pathology Journal
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• v.35 no.6
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• pp.644-653
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• 2019

The genetic variability of 'Candidatus Liberibacter asiaticus' (CLas) population associated with huanglongbing (HLB) disease of citrus in North Eastern (NE) region of India, a geographically locked region, and home for the diversity of many citrus species was analyzed on the basis of tandem repeat numbers (TRN) in variable CLIBASIA_01645 genomic loci. Fifty-five CLas strains sampled from different groves of NE Hill (NEH) region of India were in single amplicon group, but there was remarkable genetic variability in TRNs. The TRN in HLB-associated CLas strains varied from 0-21 and two novel repeat motifs were also identified. Among the NE population of CLas, TRN5 and TRN9 were most frequent (total frequency of 36.36%) followed by TRN4 (14.55%) and TRN6, TNR7 with a frequency of 12.73% each. Class II type CLas genotypes (5 < TRN ≤ 10) had highest prevalence (frequency of 60.00%) in the samples characterized in present study. Class I (TRN ≤ 5) genotypes were second highest prevalent (29.09%) in the NEH region. Further analysis of genetic diversity parameters using Nei's measure (H value) indicated wide genetic diversity in the CLas strains of NE India (H value of 0.58-0.86). Manipur CLas strains had highest genetic variability (0.86) as compared to Eastern, Southern and Central India. The R₁₀ values (TRN ≤ 10/TRN > 10) of NE CLas population was 10.43 (73/7), higher from other regions of India. Present study conclusively reported the occurrence of high genetic variability in TRN of CLas population in North East Indian citrus groves which have evolved to adapt to the specific ecological niche.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.38-38
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• 2023

Sorghum (Sorghum bicolor L.) is a promising biomass crop with a high lignocellulose content. This study aimed to select high salt-tolerance sorghum lines for cultivation on reclaimed land. Using 7-day seedlings of the sorghum population consisted of 71 radiation-derived mutants (M₂ to M₆) and 33 genetic resources, survival rate (SR), plant height (PH), root length (RL), fresh weight (FW), and chlorophyll content (CC) were measured for two weeks after 102 mM (0.6%) NaCl treatment. Furthermore, the characteristics of the sorghum population were confirmed using correlation analysis, PCA (principal component analysis), and the FCE (fuzzy comprehensive evaluation) method. Under 102 mM NaCl conditions, SR ranged from 4.9 (IS645-200-6) to 82.4% (KLSo79125-200-1), with an average of 49.9%. PH varied from 7.5 (Mesusu-100-2) to 33.2 cm (DINE-A-MITE-100-2-10), with an average of 20.4 cm. RL ranged from 1.0 (IS645-200-1) to 17.0 cm (30-100-2), with an average of 7.7 cm. FW varied from 0.1 (IS645-200-6) to 4.5 g/plant (DINE-A-MITE-100-2-10), with an average of 2.1 g/plant. CC ranged from 0.9 (DINE-A-MITE-100-2-2) to 3.1 mg/g (IS12937), with an average of 1.7 mg/g. An overall positive correlation, with SR and FW (r = 0.86, P < 0.01), and FW and CC (r = 0.79, P < 0.01), was shown by correlation analysis. Among the five traits, two principal components were extracted by PCA analysis. PC1 was significantly associated with FW, while PC2 was highly involved with RL. To evaluate the salt tolerance level of the sorghum population when an FCE based on trait data was performed, MFV (membership function value) was 0.68. As a result of compiling the MFV of each line, eight lines with MFV > 0.68 were selected. Ultimately, the radiation-derived mutant lines, DINE-A-MITE-100-2-10 and DINE-A-MITE-100-2-12 were selected as salt-tolerant sorghum lines. The results are expected to inform salt-tolerant sorghum breeding programs, and the high salt-tolerance sorghum lines might be advantageous for cultivation on reclaimed land.

• Korean Journal of Plant Resources
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• v.26 no.5
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• pp.557-565
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• 2013

This study was conducted to examine variations of 35 morphological characteristics of 6 natural populations of Zelkova serrata in Korea. On the basis of the results of the ANOVA tests, the populations were significantly different in 16 of the 20 quantitative morphological characteristics that were tested. In particular, the 'Cheongju-si Gutdae Mountain' population showed larger values in 9 of the 20 quantitative characteristics that were examined. In the results of principal component analysis of the characteristics, examined characteristics were not suitable for the variation of 6 natural populations. According to the results of cluster analysis, 'Namhae-gun Mijori Evergreen Forest' population was grouped with 'Yangpyeon-gun Yongmun Mountain' population to the nearest group, and then 'Cheongju-si Gutdae Mountain' population, 'Boeun-gun Sokri Mountain' population, 'Daegu metropolitan city Palgong Mountain' population, and 'Hamyang-gun Sang Forest' population were grouped in regular sequence.

• The Plant Pathology Journal
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• v.18 no.1
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• pp.30-35
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• 2002

The hyphal growth and biocontrol efficacy of Trichodemo harzianum in soil may depend on its interactions with biotic components of the soil environment. The effect of soil microbial biomass on growth and biocontrol efficacy of T. hanianum isolate ThzIDl-M3 (green fluorescent protein transformant) was investigated using artificially prepared different levels of soil microbial biomass (153,328, or 517ug biomass carbon per g of dry soil; BC). The hyphal growth of T. harzanum was significantly inhibited in the soil with 328 or 517 $\mu$g BC compared with 153 ug BC. When ThzIDl-M3 was added to the soils as an alginate pellet formulation, the recoverable population of ThzIDl-M3 varied, but the highest population occurred in 517ug BC. Addition of alginate pellets of ThzIDl-M3 to the soils (10 per 50 g) resulted in increased indigenous microbial populations (total fungi, bacterial fluorescent Pseudomonas app., and actinomycetes). Furthermore, colonizing ability of ThzIDl-M3 on sclerotia of Sclerotinia sclerotiorum was significantly reduced in the soil with high revel of BC. These results suggest that increased soil microbial biomass contributes to increased interactions between introduced T. harzianum and soil microorganisms, consequently reducing the biocontrol efficacy of 1T. harzianum.

• Journal of Ginseng Research
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• v.15 no.1
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• pp.31-35
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• 1991

In order to know the optimum planting density under shading structures at different light intensity, We investigated the growth status, distribution of ginseng leaf area, correlation between planting density and root weight per plant and yield, correlation between leaf area index and root weight per plant and yield. According to the increase of planting density the leaf area per plant was decreased, but leaf area index (L.A.I) was increased. Ginseng leaf population at different lines under common straw shading were distributed mainly in frost lines but polyethylene net shading at 10fo light intensity were distributed equally in all lines. Optimum planting density in common straw shading at 5% light intensity was 55 plant per tan (90 cmX180 cm) and polyethylene net shading 81 10% light intensity was 60 plant per tan, in consideration of root weight and yield. Optimum leaf area index was 2.4 under common straw shading at 5% light intensity but was 2.7 under polyethylene net shading at 10% light intensity.

• The Korean Journal of Ecology
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• v.23 no.4
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• pp.293-298
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• 2000

To study the effect of global warming on the growth of plants and plant populations throughout their life cycle under a field-like condition, we constructed a Temperature Gradient Chamber (TGC) in Tsukuba, Japan. The chamber had slender shape : 30 m long. 3 m wide, and 2.5 m high. That satisfactory performance was confirmed by a test throughout all seasons in 1998: the projected global warming condition in the near future was simulated. That is, independent of a great daily or seasonal change in ambient meteorological conditions, air temperatures at the air outlet were warmed 5$^{\circ}C$ higher than those at the ambient (the annual mean was 14.3$^{\circ}C$) with precision of ${\pm}$0.2$^{\circ}C$ (the annual means were 19.2$^{\circ}C$) with a rising rate of approximately 1$^{\circ}C$ every 5 m. This chamber will enable us to study the effects of global warming on growth of plants and plant populations because their abilities to control air temperature are excellent. TGC is expected that it would be utilized for studying the effect of global warming on plant growth under natural weather conditions.