• The Plant Pathology Journal
• /
• v.34 no.3
• /
• pp.218-235
• /
• 2018

Plant growth promoting rhizobacteria and endophytic bacteria were isolated from different varieties of turmeric (Curcuma longa L.) from South India. Totally 50 strains representing, 30 PGPR and 20 endophytic bacteria were identified based on biochemical assays and 16S rDNA sequence analysis. The isolates were screened for antagonistic activity against Pythium aphanidermatum (Edson) Fitzp., and Rhizoctonia solani Kuhn., causing rhizome rot and leaf blight diseases in turmeric, by dual culture and liquid culture assays. Results revealed that only five isolates of PGPR and four endophytic bacteria showed more than 70% suppression of test pathogens in both assays. The SEM studies of interaction zone showed significant ultrastructural changes of the hyphae like shriveling, breakage and desication of the pathogens by PGPR B. cereus (RBacDOB-S24) and endophyte P. aeruginosa (BacDOB-E19). Selected isolates showed multiple Plant growth promoting traits. The rhizome bacterization followed by soil application of B. cereus (RBacDOB-S24) showed lowest Percent Disease Incidence (PDI) of rhizome rot and leaf blight, 16.4% and 15.5% respectively. Similarly, P. aeruginosa (BacDOB-E19) recorded PDI of rhizome rot (17.5%) and leaf blight (17.7%). The treatment of these promising isolates exhibited significant increase in plant height and fresh rhizome yield/plant in comparison with untreated control under greenhouse condition. Thereby, these isolates can be exploited as a potential biocontrol agent for suppressing rhizome rot and leaf blight diseases in turmeric.

• The Plant Pathology Journal
• /
• v.29 no.2
• /
• pp.174-181
• /
• 2013

The plant growth-promoting rhizobacterium Ochrobactrum lupini KUDC1013 elicited induced systemic resistance (ISR) in tobacco against soft rot disease caused by Pectobacterium carotovorum subsp. carotovorum. We investigated of its factors involved in ISR elicitation. To characterize the ISR determinants, KUDC1013 cell suspension, heat-treated cells, supernatant from a culture medium, crude bacterial lipopolysaccharide (LPS) and flagella were tested for their ISR activities. Both LPS and flagella from KUDC1013 were effective in ISR elicitation. Crude cell free supernatant elicited ISR and factors with the highest ISR activity were retained in the n-butanol fraction. Analysis of the ISR-active fraction revealed the metabolites, phenylacetic acid (PAA), 1-hexadecene and linoleic acid (LA), as elicitors of ISR. Treatment of tobacco with these compounds significantly decreased the soft rot disease symptoms. This is the first report on the ISR determinants by plant growth-promoting rhizobacteria (PGPR) KUDC1013 and identifying PAA, 1-hexadecene and LA as ISR-related compounds. This study shows that KUDC1013 has a great potential as biological control agent because of its multiple factors involved in induction of systemic resistance against phytopathogens.

• Korean Journal of Microbiology
• /
• v.55 no.3
• /
• pp.300-302
• /
• 2019

Glutamicibacter halophytocola DR408 isolated from the rhizospheric soil of soybean plant at Jecheon showed drought tolerance and plant growth promotion capacity. The complete genome of strain DR408 comprises 3,770,186 bp, 60.2% GC-content, which include 3,352 protein-coding genes, 64 tRNAs, 19 rRNA, and 3 ncRNA. The genome analysis revealed gene clusters encoding osmolyte synthesis and plant growth promotion enzymes, which are known to contribute to improve drought tolerance of the plant.

• Journal of Ecology and Environment
• /
• v.31 no.4
• /
• pp.261-267
• /
• 2008

Growth and photosynthetic responses of one $C_3$ and two $C_4$ plants in the family Chenopodiaceae in three $CO_2$ concentration $([CO_2])$ conditions-low (about $243{\mu}mol\;mol^{-1}$, LC), present (about 378, PC), and high (about 465, HC)-were investigated in open top chambers. The relative growth rate (RGR) and net assimilation rate in the $C_3$ plant, Chenopodium album, increased with increasing $[CO_2]$, though the RGR was not enhanced significantly in the HC condition. The leaf area ratio and leaf weight ratio of the $C_3$ plant drastically decreased with increasing $[CO_2]$, suggesting that the $C_3$ plant invests more biomass to leaves in lower $[CO_2]$ conditions. The two $C_4$ plants, Atriplex glauca and A. lentiformis, showed relatively small changes in those growth parameters. These photosynthetic-pathway-dependent responses suggest that growth patterns of $C_3$ and $C_4$ plants have been altered by past increases in atmospheric $[CO_2]$ but that there will be relatively little further alteration in the future high-$CO_2$ world.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.156-156
• /
• 2017

Plants are grown under constitutive changing of environmental conditions and response to external conditions at both protein and transcription level. The effects of heat on plant growth are broad and influence the yield directly. Heat stresses could be classified depend on intensity and duration. Fundamental changes of growth condition by climate change maybe or maybe not classified as a stress on plant growth. The effects of a short and unanticipated impact of elevated heat on plant could be different with those of under longer extension of ambient heat. To examine differently expressed gene sets by ambient heat stress of soybean, we grow the soybean in normal condition for three weeks. After that, soybean plants move to growth chamber. The temperature of growth chamber increase up to $9^{\circ}C$ for four days. We have extracted mRNA and micro RNA every 24 hours and carried RNA sequence analysis. We found major metabolic pathways affected by ambient heat stress. Mainly carbon metabolism, translation machinery and amino acid synthesis are affected. We discussed the expression patterns of genes of heat sensing and hormone responses.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.33 no.6 s.113
• /
• pp.78-89
• /
• 2006

This study was conducted to analyze the planting structure and ecological characteristics of greon spaces on the grounds of e Dangjin steam power plant. To achieve these goals, we surveyed existing vegetation, plant community structure, Plant volume and growth rate. Based on e vegetation analysis, existing vegetation was classified into six types: herbaceous species $(70.54\%)$, evergreen coniferous trees $(21.17\%)$, deciduous broad-leaved trees $(5.10\%)$, deciduous coniferous trees $(1.47\%)$, shrubs $(0.12\%)$, and other types $(1.59\%)$. The coal storage, office, and playground areas were community is the natural forest area where a Pinus thunbergii / Pinus densiflora community is distributed in terms of vegetation structure, species diversity, plant volume, and growth rate. The artificial green spaces(near the coal storage, office, playground areas) had a single-layer structure. Species diversity indices of the artificial green areas were $0.1655\~0.4807$ compared to 0.8628 in the natural forest, which presented a good growth environment. Also, the plant volume in the artificial green space was lower than that of the natural green space. Therefore, it would be desirable to develope a multi-layer structure similar to that of the vegetation in the natural green space in order to improve the amount of plant volume. The plant-damage ratio of Pinus thunbergii was $52.48\%$ in the coal storage area, and $8.48\~ 11.52\%$, in the other survey areas. Also, the vitality of Pinus thunbergii was $15.45k{\Omega}$ in the coal storage areas, which indicates bad growing conditions. This suggests that soil characteristics and dust have a bad impact on growth. The investigation into deciduous tres' growth status showed that appropriate plants would be Albizzia julibrissin, Acer palmatum var. sanguineum, Acer palmatum, Malus spp., Prunus sargentii.

• Journal of Environmental Science International
• /
• v.20 no.9
• /
• pp.1183-1190
• /
• 2011

This study was aimed to establish the evaluating protocol and standard assessment for genetically modified (GM) hot pepper and to find out a proper statistic method to analyze for equality of agricultural characters between GM and non-GM pepper lines. GM and non-GM hot pepper lines were cultivated in two GMO fields in the middle region of Korea and total of 52 agricultural characters were collected during the plant growing season for 4 years, 2007 to 2010. Levene's test was conducted to confirm the homogeneity of raw data before statistic analysis. Two-way ANOVA in the multivariate tests and t-test were conducted to analyze 52 agricultural characters in order to find out the equality between H15 and P2377. From the statistical analysis through two-way ANOVA, 16 out of 16 plant growth traits, 9 out of 18 green fruit traits and 7 out of 18 red fruit traits among 4 years and 9 out of 16 plant growth traits, 4 out of 18 green fruit traits and 3 out of 18 red fruit traits between H15 and P2377 have shown the statistic differences. With the same raw data of 52 agricultural characters, t-test was also conducted. Based on the result from t-test, only 1 out of 16 plant growth traits, 2 out of 18 green fruit traits and 1 out of 18 red fruit traits have shown the differences between H15 and P2377, so that it was concluded that there is no statistic difference between H15 and P2377 in terms of agricultural characters. Also, the t-test is a proper statistic method to analyze each trait between GM and its control lines in order to evaluate agricultural characters.

• BMB Reports
• /
• v.39 no.3
• /
• pp.297-303
• /
• 2006

As lipolytic enzymes, GDSL lipases play an important role in plant growth and development. In order to identify their functions and roles, the full-length cDNA of a GDSL lipase gene, designated BnLIP2, was isolated from Brassica napus L. BnLIP2 was 1,300 bp long, with 1,122 bp open reading frame (ORF) encoding 373 amino acid residues. Sequence analysis indicated that BnLIP2 belonged to GDSL family. Southern blot analysis indicated that BnLIP2 belonged to a small gene family in rapeseed genome. RT-PCR analysis revealed that BnLIP2 was a tissue-specific expressing gene during reproductive growth and strongly expressed during seed germination. BnLIP2 expression could not be detected until three days after germination, and it subsequently became stronger. The transcript of this gene was deficient in root of seedlings growing at different stages. When juvenile seedlings were treated by methyl jasmonate (MeJ), salicylic acid (SA) and naphthalene acetic acid (NAA), BnLIP2 expression could not be induced in root. Our study implicates that BnLIP2 probably plays an important role in rapeseed germination, morphogenesis, flowering, but independent of root growth and development.

• The Plant Pathology Journal
• /
• v.23 no.3
• /
• pp.223-225
• /
• 2007

Many plant growth-promoting rhizobacteria (PGPRs) have been known for beneficial effects on plants including biological control of soilborne pathogens, induced systemic resistance to plant pathogens, phytohormone production, and improvement of nutrient and water uptake of plants. We developed a simple and rapid method for screening potential PGPR, especially phytohormone producing rhizobacteria, or for analyzing their functions in plant growth using cucumber seedling cuttings. Surface-sterilized cucumber seeds were grown in a plastic pot containing steamed vermiculite. After 7 days of cultivation, the upper part 2 cm in length of cucumber seedling, was cut and used as cucumber cuttings. The base of cutting stem was then dipped in a microcentrifuge tube containing 1.5ml of a bacterial suspension and incubated at $25^{\circ}C$ with a fluorescent light for 10 days. Number and length of developed adventitious roots from cucumber cuttings were examined. The seedling cuttings showed various responses to the isolates tested. Some isolates resulted in withering at the day of examination or in reduced number of roots developed. Several isolates stimulated initial development of adventitious roots showing more adventitious root hair number than that of untreated cuttings, while some isolate had more adventitious root hair number and longer adventitious roots than that of untreated control. Similar results were obtained from the trial with rose cuttings. Our results suggest that this bioassay method may provide a useful way for differentiating PGPR's functions involved in the development of root system.

• Korean Journal Plant Pathology
• /
• v.14 no.3
• /
• pp.203-208
• /
• 1998

We isolated and characterized an antifungal peptide from the seeds of Phytolacca americana. Growth inhibition assay with Botrytis cinerea was used to screen inhibitory proteins from 60 different plant species. A 4 kDa antifungal peptide (Pa-AFP) inhibitory to hyphal growth of B. cinerea was found in the seeds of P. americana. The peptide Pa-AFP was purified to homogeneity by chromatographies of Sephadex G-50, DEAE-Sepharose, Sephacryl S-300, and C18 reverse-phase HPLC. Western blot analysis showed that a polyclonal antibody raised against the purified peptide cross-reacted with a 4 kDa protein in seeds but not in root and leaf tissues of P. americana. Pa-AFP inhibited the hyphal growth of Botrytis cinerea, Rihzoctonia solani, Fusarium oxysporum, and Magnaporthe grisea. Pa-AFP exhibited growth inhibition of Saccharomyces cerevisiae strain BWG7a, which was sensitive to osmotin.