• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.74-74
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• 2022

본 연구는 붉나무의 기능성 성분의 산업화 활용을 위한 고품질의 붉나무 1년생 용기묘 대량생산을 위해 실시되었다. 붉나무 1년생 용기묘의 묘목품질의 향상을 위해 다양한 농도의 시비처리하여 우량묘 생산에 적합한 시비수준을 구명하고자 하였다. 시비실험은 무시비구 포함하여 수용성 복합비료 (N:P:K=20:20:20, v/v)를 1,000mg·L^-1, 2,000mg·L^-1, 3,000mg·L^-1 수준으로 실시하였다. 시비처리는 붉나무 용기묘의 간장과 근원경 생장은 1,000mg·L^-1 시비처리구에서 생장이 가장 좋게 나타났으며 시비수준이 증가할수록 감소하는 경향을 보였다. 뿌리형태 특성분석에서도 전체뿌리길이, 투영단면적, 표면적 및 뿌리부피가 간장과 근원경 생장과 동일한 경향을 보이는 것으로 조사되었다. 건물 생산량은 부위별 잎, 줄기, 뿌리 및 전체가 모두 1,000mg·L^-1 시비처리구에서 가장 높았으며, 그 이상의 시비수준에서는 점점 감소하는 경향을 보였다. H/D(Hight/Root collar diameter)율은 전체가 3.82(무시비구) ~ 4.90, T/R(Top/Root)율은 전체가 0.56(1,000mg·L^-1 시비처리구) ~ 0.82로 조사되었다. 시비처리 에 의한 붉나무의 LWR(Leaf dry weight ratio)은 무시비구, SWR(Shoot dry weight ratio)은 3,000mg·L^-1 시비처리구, 그리고 RWR(Root dry weight ratio)은 1,000mg·L^-1 시비처리구에서 유의적 차이를 보이며 높게 나타났다. 묘목품질지수(QI)의 경우 1,000mg·L^-1 시비처리구에서 12.13으로 다른 시비수준들보다 월등히 높았다. 본 실험의 결과를 종합하면, 뿌리발달이 좋은 우량한 붉나무 생산에 적절한 시비수준은 1,000mg·L^-1 정도인 것으로 판단된다.

• The Plant Pathology Journal
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• 제30권1호
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• pp.90-95
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• 2014

This study was conducted to evaluate the resistance of 212 accessions of lettuce germplasm to gray mold disease caused by Botrytis cinerea. The lettuce germplasm were composed of five species: Lactuca sativa (193 accessions), L. sativa var. longifolia (2 accessions), L. sativa var. crispa (2 accessions), L. saligna (2 accessions), and L. serriola (1 accession); majority of these originated from Korea, Netherlands, USA, Russia, and Bulgaria. After 35 days of spray inoculation with conidial suspension ($3{\times}10^7$ conidia/ml) of B. cinerea on the surface of lettuce leaves, tested lettuce germplasm showed severe symptoms of gray mold disease. There were 208 susceptible accessions to B. cinerea counted with 100% of disease incidence and four resistant accessions, IT908801, K000598, K000599, and K021055. Two moderately resistant accessions of L. sativa, K021055 and IT908801, showed 20% of disease incidence of gray mold disease at 45 days after inoculation; and two accessions of L. saligna, K000598 and K000599, which are wild relatives of lettuce germplasm with loose-leaf type, showed complete resistance to B. cinerea. These four accessions are candidates for breeding lettuce cultivars resistant to gray mold disease.

• 한국자원식물학회지
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• 제23권3호
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• pp.233-241
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• 2010

In vitro elimination of Sweet potato leaf curl virus (SPLCV) from infected sweet potato is difficult due to low number of virus-free plants obtained from meristem tip culture and long growth period required for the virus detection. In this study, efficient production of the SPLCV-free sweet potato by in vitro therapy coupled with a PCR assay for virus detection was investigated. Infected shoots cultured on Murashige and Skoog medium were treated at three different temperatures for 7 weeks followed by meristem tip culture on the medium with or without ribavirin at 50 mg/L. The regenerated plantlets were tested for virus infection by a PCR assay. The results showed that the both heat- and cold-treatments, and addition of the ribavirin did not have significant effect on efficiency of the virus elimination. The meristem size, however, greatly affected the survival rate. Meristems sized over 0.4 mm survived better than smaller ones (0.2-0.3 mm). The PCR assay was approved to be a rapid, sensitive and reliable for the SPLCV detection in regenerated plantlets. Therefore, combination of cultivating meristem tips sized 0.4-0.5 mm on the medium at $22^{\circ}C$ without ribavirin and detection of SPLCV in the regenerated plantlets by the PCR assay was an efficient system for the SPLCV elimination from infected sweet potato.

• 한국자원식물학회지
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• 제29권6호
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• pp.679-689
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• 2016

Lettuce is an important annual leafy vegetable and bitterness is its potent flavor character. Lettuce germplasm differ their phenotypic characters and sesquiterpene lactones (SLs) contents which are important for consumer's acceptance. This study was carried out to evaluate the phenotypic characters and SLs contents in one hundred lettuce germplasm in Jeonju, Korea. Twenty-three agro-morphological (16 qualitative and 7 quantitative) traits and two SLs (lactucin and lactucopicrin) contents were studied in these germplasm. Germplasm exhibited the variation in qualitative and quantitative characters. Average plant weight was 423.9 g with a range from 116.0 to 905.0 g. Lactucin content was varied from 19.7 (IT 294226) to $194.4{\mu}g/g$ (IT 294298) with an average concentration of $84.7{\mu}g/g$. Lactucopicrin ranged from 82.5 (IT 300134) to $2228.6{\mu}g/g$ (IT 294210) with an average concentration of $586.3{\mu}g/g$. Total SLs content was ranged from 120.1 (IT 300134) to 2286.6 (IT 294210)${\mu}g/g$ with the average concentration of $671.0{\mu}g/g$. Significant ($p{\leq}0.05$) differences were found between crisp head and butter head germplasm for lactucin, lactucopicrin and total SLs content. Crisp head germplasm revealed the highest average lactucin ($112.9{\mu}g/g$), lactucopicrin ($734.8{\mu}g/g$) and total SLs content ($847.7{\mu}g/g$). Crisp head and leafy type germplasm exhibited more total SLs content (847.7 and $744.7{\mu}g/g$, respectively) than cos ($524.9{\mu}g/g$) and butter head type ($519.4{\mu}g/g$). Principal component analyses of the quantitative traits indicated that the first principal component axis accounted more than 91% of the total variation. This study revealed the ample genetic variation in the agro-morphological traits and SLs contents to support the selection for improved lettuce varieties.

• 한국자원식물학회지
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• 제29권6호
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• pp.658-669
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• 2016

Rice blast, caused by a fungus Magnaporthe oryzae, is one of the most devastating diseases of rice worldwide. Analyzing the valuable genetic resources is important in making progress towards blast resistance. Molecular screening of major rice blast resistance (R) genes was determined in 2,509 accessions of rice germplasm from different geographic regions of Asia and Europe using PCR based markers which showed linkage to twelve major blast R genes, Pik-p, Pi39, Pit, Pik-m, Pi-d(t)2, Pii, Pib, Pik, Pita, Pita/Pita-2, Pi5, and Piz-t. Out of 2,509 accessions, only two accessions had maximum nine blast resistance genes followed by eighteen accessions each with eight R genes. The polygenic combination of three genes was possessed by maximum number of accessions (824), while among others 48 accessions possessed seven genes, 119 accessions had six genes, 267 accessions had five genes, 487 accessions had four genes, 646 accessions had two genes, and 98 accessions had single R gene. The Pik-p gene appeared to be omnipresent and was detected in all germplasm. Furthermore, principal component analysis (PCA) indicated that Pita, Pita/Pita-2, Pi-d(t)2, Pib and Pit were the major genes responsible for resistance in the germplasm. The present investigation revealed that a set of 68 elite germplasm accessions would have a competitive edge over the current resistance donors being utilized in the breeding programs. Overall, these results might be useful to identify and incorporate the resistance genes from germplasm into elite cultivars through marker assisted selection in rice breeding.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.52-52
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• 2019

Buckwheat is well-known crop for containing a high contents of flavonoids that are effective in vascular disease. The current study was performed to estimate the influence of morphological characterization of Fagopyrum esculentum (ES) germplasm for seed's two major flavonoids contents: rutin and quercetin. We found that the red stem color, pale green leaf color, arrowhead leaf shape, white flower color, pale brown seed coat color, and egg-shaped seed were significantly associated with 77%, 56.7%, 83.7%, 98.7%, 70.8% and 74.5% germplasm, respectively. Overall, the rutin contents of ES germplasm ranged from 0.30 to 47.86 mg/100g dry weight (DW) and the quercetin contents ranged from 0 to 1.22 mg/100g DW. The rutin contents of germplasm possessing red stem color, pale green leaf color, arrowhead leaves, white flower color, pale brown seed coat color and egg-shaped seed ranged from 7.22 to 47.86 mg/100g DW. However, the quercetin contents of germplasm with red stem color and pale brown seed coat color ranged from 0 to 1.15 mg/100g DW, with pale green leaves ranged from 0 to 0.96 mg/100g, with arrowhead leaves and white flower ranged from 0 to 1.22 mg/100g and with egg-shaped seed ranged from 0.32 to 1.22 mg/100g DW. In PCA analysis, the first three principal components (PCs) showed Eigen value more than 1 and accounted for 51.70% of variation. For both higher contents of rutin and quercetin, the morphological evaluation in ES shows a tendency of red stem color, arrowhead leaves, pale green leaf color, white flower color, pale brown seed coat color and egg-shaped seed. From this information, we can assume the rutin and quercetin contents by the morphological characteristics of the germplasm. And It could be useful in improving the rutin and quercetin contents and selecting proper resources for cultivation in existing buckwheat cultivars.

• Plant Biotechnology Reports
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• 제2권3호
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• pp.191-198
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• 2008

Germplasm characterization and evolutionary process in viable populations are important links between the conservation and utilization of plant genetic resources. Here, an investigation is made, based on molecular and biochemical techniques for assessing and exploiting the genetic variability in germplasm characterization of taro, which would be useful in plant breeding and ex situ conservation of taro plant genetic resources. Geographical differentiation and phylogenetic relationships of Indian taro, Colocasia esculenta (L.) Schott, were analyzed by random amplified polymorphic DNA (RAPD) and isozyme of seven enzyme systems with specific reference to the Muktakeshi accession, which has been to be proved resistant to taro leaf blight caused by P. colocasiae. The significant differentiations in Indian taro cultivars were clearly demonstrated by RAPD and isozyme analysis. RAPD markers showed higher values for genetic differentiation among taro cultivars and lower coefficient of variation than those obtained from isozymes. Genetic differentiation was evident in the taro accessions collected from different regions of India. It appears that when taro cultivation was introduced to a new area, only a small fraction of genetic variability in heterogeneous taro populations was transferred, possibly causing random differentiation among locally adapted taro populations. The selected primers will be useful for future genetic analysis and provide taro breeders with a genetic basis for selection of parents for crop improvement. Polymorphic markers identified in the DNA fingerprinting study will be useful for screening a segregating population, which is being generated in our laboratory aimed at developing a taro genetic linkage map.

• 한국자원식물학회지
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• 제29권4호
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• pp.479-494
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• 2016

기계수확에 적합하고 고기능성 유전자원을 선발하여 중부지역에 재배품종자원으로 공급하기 위하여 수수 100 자원을 공시하여 실시한 시험결과는 다음과 같다. 황금찰과 남풍찰을 대조 품종으로 하여 수집된 수수 100 자원의 작물학적 특성을 검정한 결과 출수소요일은 68~94일의 변이로 변이가 컸으며, 이삭형태는 7종류의 형태로 중간형이 가장 많이 나타났다. 간장은 70~429 ㎝의 변이로 변이 폭이 컸으며, 평균 260 ㎝였고, 100~150 ㎝가 31%로 최빈수였다. 이삭길이는 17~44.4 ㎝의 변이를 보였으며, 평균은 28.8 ㎝였다. 경태는 16.6~29.7 ㎜의 변이로 변이 폭이 컸으며, 평균은 21.4 ㎜였고, 엽수는 6.3~20개의 변이로 변이 폭이 컸으며, 평균은 11.4개였다. 천립중은 14.55~32.54 g의 변이로 변이 폭이 컸으며, 평균은 22.14 g이었고, 이삭당 수량은 18.32~44.96 g의 변이로 변이 폭이 컸으며, 평균은 30.74 g이었다. 작물학적 특성 검정을 통해 기계수확에 적합한 150 ㎝ 이하의 단간종이며, 이삭당 수량이 대조품종보다 높은 33.63 g 이상인 18 유전자원을 선발하였다. 선발된 18 유전자원 중 기능성물질 함량이 높은 자원 선발을 위하여 총 폴리페놀 함량, 전자공여능 및 총 안토시아닌 함량을 분석한 결과, 총 폴리페놀 함량은 대조품종인 황금찰은 174.08 ㎍ GAE/g이었으며, 남풍찰은 292.30 ㎍ GAE/g이었고, DS017, DS019, DS020, DS027, DS066이 대조품종인 남풍찰보다 함량이 높았다. 전자공여능은 대조품종인 황금찰이 26.25%였으며, 남풍찰은 33.65%였고, DS017, DS019, DS020, DS027, DS066, DS068, DS236이 대조품종인 남풍찰보다 소거능이 높거나 유사하였다. 총 안토시아닌 함량은 대조품종인 황금찰은 49.22 ㎍/g이었으며, 남풍찰은 51.52 ㎍/g이었고, DS488, DS428이 대조품종인 남풍찰과 황금찰보다 함량이 높거나 유사하였다. 그리고 항산화능과 항산화 성분과의 상관관계를 선형회귀분석 한 결과, 총 폴리페놀 함량과 DPPH radical 소거능 사이에 99.9% 수준으로 R=0.922의 높은 상관관계가 나타나 총 폴리페놀 함량이 높을수록 DPPH radical 소거능이 증가함을 알 수 있었다. 작물학적 특성 검정과 기능성물질 함량분석을 통하여 기계수확에 적합한 단간종이며, 이삭당 수량이 높고, 기능성물질 함량이 높은 DS017, DS019, DS020, DS027, DS066 5자원을 선발하였다.

• 한국자원식물학회지
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• 제25권6호
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• pp.762-772
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• 2012

The objective of this study was to analyze the genetic diversity using SSR marker and investigate the fatty acid composition of perilla (P. frutescens var. frutescens) germplasm. Genetic diversity among 95 accessions, which consisted of 29 weedy types and 66 landrace accessions, was evaluated based on 12 SSR markers carrying 91 alleles. The mean values of observed ($H_O$) and expected heterozygosities ($H_E$) were 0.574 and 0.640, respectively, indicating a considerable amount of polymorphism within this collection. A genetic distance-based phylogeny grouped into two distinct groups, which were the landrace, moderate and weedy type, genetic distance (GD) value was 0.609. The physicochemical traits about crude oil contents and fatty acid compositions were analyzed using GC. Among tested germplasm, the total average oil contents (%) showed a range from 28.57 to 49.67 %. Five fatty acids and their contents in the crude oils are as follows: ${\alpha}$-linolenic acid (41.12%-51.81%), linoleic acid (15.38%-16.43%), oleic acid (18.93%-27.28%), stearic acid (2.56%-4.01%), and palmitic acid (7.38%-10.77%). The average oil content of wild types was lower than landrace, and the oil content of middle genotype accessions was higher than other germplasm, but no significant variation between landrace and wild types was shown. Nevertheless, IT117174, landrace of Korea, was highest in crude oil content (47.11%) and linolenic acid composition (64.58%) among the used germplasm. These traits of the selected accessions will be helped for new functional plant breeding in perilla crop.

• 한국작물학회지
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• 제57권4호
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• pp.389-396
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• 2012

Melon (Cucumis melo) is an annual herbaceous plant of the family Cucurbitaceae. Phytophthora rot, caused by Phytophthora capsici is a serious threat to cucurbits crops production as it directly infects the host plant, and it is difficult to control because of variable pathogenicity. This study investigated the resistance of 450 accessions of melon germplasm against Phytophthora rot by inoculating the seedlings with sporangial suspension ($10^{5\;or\;6}$ zoosporangia/ml) of P. capsici. Disease incidence of Phytophthora rot was observed on the melon germplasm at 7-day intervals for 35 days after inoculation. Susceptible melon germplasm showed either severe symptoms of stem and root rot or death of the whole plant. Twenty out of 450 tested accessions showed less than 20% disease incidence, of which five accessions showed a high level of resistance against Phytopthtora rot. Five resistant accessions, namely IT119813, IT138016, IT174911, IT174927, and IT906998, scored 0% disease incidence under high inoculum density of P. capsici ($10^6$ zoosporangia/mL). We recommend that these candidate melon germplasm may be used as genetic resources in the breeding of melon varieties resistant to Phytophthora rot.