• Journal of Microbiology and Biotechnology
• /
• 제16권3호
• /
• pp.491-495
• /
• 2006

Methanol extracts of fruits of 67 plants were screened for in vivo antifungal activity against Magnaporthe grisea, Corticium sasaki, Botrytis cinerea, Phytophthora infestans, Puccinia recondita, and Blumeria graminis f. sp. hordei. Among them, 13 plant extracts ($3,000\;{\mu}g/ml$) showed more than 90% disease-control efficacy against at least one of six plant diseases. Specifically, the extracts of Aleurites fordii, Angelica dahurica, Camellia japonica, Chamaecyparis pisifera, Pittosporum tobira, and Styrax japonica controlled more than 90% of the development of rice blast at $1,000{\mu}g/ml$. Extracts of both S. japonica and A. dahurica fruits at $333{\mu}g/ml$ concentration displayed strong antifungal activity against M. grisea on rice seedlings.

• The Plant Pathology Journal
• /
• 제28권3호
• /
• pp.332-332
• /
• 2012

• 식물병연구
• /
• 제17권3호
• /
• pp.410-412
• /
• 2011

2000년대 후반부터 고흥지역의 비파나무(Eriobotrya japonica)와 제주지역의 홍가시나무(Photinia glabra)에 Entomosporium mespili에 의한 극심한 점무늬병이 발생하기 시작하였다. 따라서 방제효과가 높은 살균제를 선발하기 위하여 이 연구를 수행하였다. 효과를 검정한 3개의 농약 중 비파나무 점무늬병에는 propiconazole 처리가 방제가 64.1%로 가장 높게 나타났고, 홍가시나무 점무늬병에는 chlorothalonil 처리가 방제가 87.6%로 가장 높게 나타났다. Thiopanate-methyl 처리는 방제가가 비교적 낮게 나타났고, propiconazole은 홍가시나무에 약해를 나타내었다.

• 한국응용곤충학회지
• /
• 제31권3호
• /
• pp.289-303
• /
• 1992

Investigation of the environmental impact of widespread pesticide use has revealed a virtue/vice relationship. Although many pesticides perform their function and disappear without harm to the environment, others persist beyond their useful purpose and cause direct of indirect hazard to man, domestic animals and wildlife. Concurrently, many pests have rapidly adjusted to chemical control practices through changes in behavior that avoid exposure to pesticides of throuth genetic selection for populations resistant to the toxicants. The prospect of losing control over insect herbivores and desease vectors and returning to the days of global hunger and disease is unthinkable. Fortunately, from basic studies of insect and plant biology many opportunities for the development of safe, selective and environmentally pacific strategies for insect pest management are being realized.

• The Plant Pathology Journal
• /
• 제29권3호
• /
• pp.344-349
• /
• 2013

Intraspecific variation in morphometry of pine wood nematodes Bursaphelenchus xylophilus and B. mucronatus in relation to geographical locations in South Korea was investigated using morphometric characters (body length, a, b and c ratio, stylet length, and spicule length for a male nematode and V (%) value for a female nematode). B. xylophilus was isolated from Pinus thunbergii in Jinju (1998), Ulsan (2000), Yangsan (2000), Mokpo (2001) and Jeju (2004), and from P. densiflora in Gumi (2001). B. mucronatus was isolated from P. thunbergii in Jinju (1991) and from P. densiflora in Milyang (2001). The body length of male and female B. xylophilus had the highest coefficient of variability and showed significant differences among geographical locations. The V (%) value for female B. xylophilus showed the lowest coefficient of variability, changing little with geographical area and host plant. All morphometric characters in B. mucronatus except for stylet length and female body length showed no significant differences between locations or hosts, suggesting they may not be affected by geographical area or host plant.

• The Plant Pathology Journal
• /
• 제34권6호
• /
• pp.459-469
• /
• 2018

Plants and microorganisms (microbes) use information from chemicals such as volatile compounds to understand their environments. Proficiency in sensing and responding to these infochemicals increases an organism's ecological competence and ability to survive in competitive environments, particularly with regard to plant-pathogen interactions. Plants and microbes acquired the ability to sense and respond to biogenic volatiles during their evolutionary history. However, these signals can only be interpreted by humans through the use of state-of the-art technologies. Newly-developed tools allow microbe-induced plant volatiles to be detected in a rapid, precise, and non-invasive manner to diagnose plant diseases. Beside disease diagnosis, volatile compounds may also be valuable in improving crop productivity in sustainable agriculture. Bacterial volatile compounds (BVCs) have potential for use as a novel plant growth stimulant or as improver of fertilizer efficiency. BVCs can also elicit plant innate immunity against insect pests and microbial pathogens. Research is needed to expand our knowledge of BVCs and to produce BVC-based formulations that can be used practically in the field. Formulation possibilities include encapsulation and sol-gel matrices, which can be used in attract and kill formulations, chemigation, and seed priming. Exploitation of biogenic volatiles will facilitate the development of smart integrated plant management systems for disease control and productivity improvement.

• 식물병연구
• /
• 제17권1호
• /
• pp.95-98
• /
• 2011

국내 당근 유기재배를 위한 저항성 품종 선발을 목적으로 한 실험에서는 당근 유전자원 32품종을 이용하여 병저항성(흰가루병, 검은무늬병, 검은잎마름병) 검정, 내충성 및 추대 정도를 조사하였다. 흰가루병 스크리닝 결과 'PI 223360'이 저항성이었으며, 'Oxheart Carrot Heirloom'를 포함한 10개의 품종이 중도저항성이었고 'Long Impeator #58'을 포함한 22품종이 감수성인 것으로 나타났다. 당근 검은무늬병과 검은잎마름병의 스크리닝 결과 모든 품종이 저항성을 나타내진 않았지만, 몇몇 품종이 중도저항성을 가진 것으로 확인되었다. 내충성을 조사한 결과 'Oxheart Carrot Heirloom'를 포함한 13품종이 내충성인 것으로 조사되었으며, 'Nantes Coreless Carrot'을 포함한 11개의 품종들이 중간정도를 나타냈으며 'SA 102'와 'Scarlet Keeper Carrot Rare'은 감수성인 것으로 조사되었다. 추대조사 결과에서는 'Hongsim Ouchon Carrot'을 포함한 6개의 품종이 이른 추대를 보였으며, 'Japanese Imperial Long Carrot'를 포함한 4개 품종이 중간 추대를 나머지는 만추대성인 것을 확인하였다. 이상의 결과를 근거로 'Oxheart Carrot Heirloom'이 조사한 32개 당근 유전자원 중 유기재배를 위한 품종으로 선발되었다.

• 식물병연구
• /
• 제10권4호
• /
• pp.279-284
• /
• 2004

경상남도 진주와 하동지역의 산초나무 재배단지에서 잎녹병에 의한 피해가 매년 지속적으로 발생하고 있다. 산초나무 잎에 형성된 여름포자세대의 녹포자세대 기주를 밝히고 녹병균을 동정하기 위하여 본 병해의 발생지 주변 곰솔에서 채집된 녹포자를 산초나무에 인공접종하였다. 그 결과 산초나무에서 성공적으로 여름포자가 형성되어 이 녹병균은 녹포자세대를 곰솔에서, 여름포자 및 겨울포자세대를 산초나무에서 형성하는 장세대형 생활사를 갖는 C. xanthoxyli로 동정하였다. 대부분의 형태적 형질은 기존 기록과 일치하였으나 녹포자와 여름포자의 표면돌기 수에는 차이가 있었다. 곰솔과 산초나무에서 채집된 C. xanthoxyli의 건조표본을 이용하여 녹포자세대 및 여름포자세대의 형태학적 특징을 기술하였다. 경남 진주와 하동지역에서 조사된 산초나무에서의 최초 병발생시기는 6월 하순${\sim}$7월 초순이었으며 8월 중의 이병엽율은 17.8%${\sim}$58.7%이었다.

• Journal of Microbiology and Biotechnology
• /
• 제25권11호
• /
• pp.1960-1965
• /
• 2015

Rabbit hemorrhagic disease virus (RHDV) is highly contagious and often causes fatal disease that affects both wild and domestic rabbits of the species Oryctolagus cuniculus. A highly pathogenic RHDV variant (RHDVa) has been circulation in the Korean rabbit population since 2007 and has a devastating effect on the rabbit industry in Korea. A highly pathogenic RHDVa was isolated from naturally infected rabbits, and the gene encoding the VP60 protein was cloned into a baculovirus transfer vector and expressed in insect cells. The hemagglutination titer of the Sf-9 cell lysate infected with recombinant VP60 baculovirus was 131,072 units/50 μl and of the supernatant 4,096 units/50 μl. Guinea pigs immunized twice intramuscularly with a trial inactivated RHDVa vaccine containing recombinant VP60 contained 2,152 hemagglutination inhibition (HI) geometric mean titers. The 8-week-old white rabbits inoculated with one vaccine dose were challenged with a lethal RHDVa 21 days later and showed 100% survival rates. The recombinant VP60 protein expressed in a baculovirus system induced high HI titers in guinea pigs and rendered complete protection, which led to the development of a novel inactivated RHDVa vaccine.

• The Plant Pathology Journal
• /
• 제24권2호
• /
• pp.159-163
• /
• 2008

Accurate identification of pine wood nematode, Bursaphelenchus xylophilus is a prerequisite to diagnose the pine wilt disease. However, a fungivorous nematode, B. mucronatus is highly similar to B. xylophilus and it is difficult to differentiate these two species by morphological features. A molecular diagnosis method, ITSRFLP was applied for the identification of B. xylophilus and B. mucronatus from Korea. Genomic DNA was extracted from a single individual nematode and ITS DNA was amplified by PCR. The size of PCR product was approximately 900bp and the sequence data were obtained after cloning. Amplified ITS was digested by 5 different restriction enzymes (Rsa I, Hae III, Msp I, Hinf I, and Alu I) and provided a discriminatory profile for B. xylophilus and B. mucronatus. Besides, B. mucro- natus was determined to have 2 different genotypes, East Asian type and European type also clearly separated by Rsa I and Hae III digestion. European type of B. mucronatus is recently collected from Pinus koraiensis and has not been reported before. ITS sequnce data were analyzed by Restriction Mapper program and the result supported ITS-RFLP pattern. These data indicated that PCRRFLP method is an accurate and simple way for identification of Bursaphelenchus species.