• Journal of environmental and Sanitary engineering
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• v.21 no.2 s.60
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• pp.1-10
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• 2006

Codonopsis pilosula and Adenophora triphylla were cultivated by sawing seeds, but the germination rate were low. To cultivate these two wild plants was very difficult. The marketable roots of the plants had low quality. The plant pathogens were very difficulty to prevent the diseases (Rhizoctonia, Pythium, Fusarium, Erwinia, Botrytis, Phythophthora) appeared in cultural state. For the extermination of the disease needs a lot of agricultural chemicals, the effect of remain behind an insecticides was high of a hazardous rate after harvest. On this studies, for the safe prevention of the diseases and the promote of seeds germination, we used Bacillus subtilis, B. liquefaciens, Paenibacillus polymixa, Pseudomonas putida separated in our research, these results would bring us harmless products of medicinal herbs for human.

• Korean Journal of Pharmacognosy
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• v.1 no.2
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• pp.29-30
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• 1970

Sugar beet represents the root of Beta vulgaris L. var. rapa $D_{UMORT}$. of the family, Chenopodiaccae. Sugar beet has been generally cultivated in the northern temperate region, but in our country, there has been seen no production of sugar from the cultivated beet so far We obtained the seeds of sugar beet from Canada and began to cultivate them in our N.I.H. Medicinal Plant Garden from April, 1969. We found such successful 90% germination, where with in October obtained 1kg root per stub which contained $16{\sim}20%$ of sugar.

• Journal of Bio-Environment Control
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• v.30 no.4
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• pp.257-262
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• 2021

The pinning method is the traditional method to produce strawberry transplants. But, cultivating the pinning transplant is a labor-intensive operation and needs a long labor period. The cutting method has been considered as an effective alternative to the pinning method, due to the relatively short labor period and works time. This study was conducted to investigate the labor period, work time, and growth between pinning and cutting methods for strawberry transplants. The 'Maehyang' strawberry was cultivated at each pinning and cutting strawberry greenhouses. The time for special works on pinning method (pinning work, elimination of mother plant, and division of daughter plant), and cutting method (cutting collection, pretreatment before storage, and cutting work) were measured. The pinning method needed 6 tasks (planting of mother plant, maintaining of mother plant, pinning work, maintaining of daughter plant, elimination of mother plant, and division of daughter plant) for 158 days, and cutting method needed 4 tasks (collection and storage of cutting, cutting work, misting, and maintaining of transplants) for 113 days to cultivate transplants for fruit. And pinning method needed more work time than the cutting method. There was no significant difference between the growth of pinning and cutting transplants. These results showed that the cutting method saved more labor period, work time than pinning during the nursery period without losing transplant quality.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.105-105
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• 2018

Finger millet, Eleusine coracana Gaertn., is more nutritious than other cereals and millets and widely cultivate in tropical regions of the world. However, status of its genetic diversity remained concealed due to lack of research work in this species. In recent years, microsatellites have become the most used markers for studying population genetic diversity. In present study, genetic diversity and structure of different populations of finger millet from Africa and South Asia was examined at molecular level using newly developed EST-Simple Sequence Repeat (EST-SSR) markers using a total of 1,927 ESTs of Eleusine coracana available in the NCBI database. In total, 46 primers produced 292 alleles in a size range of 100-500 bp and mean Polymorphism Information Content (PIC) and Marker Index (MI) were 0.372 and 1.04, respectively. 46 primers showed polymorphism and 21 primers were identified as having a PIC value above 0.5. Principal coordinates analysis and the dendrogram constructed out of combined data of both markers showed grouping of finger millet accessions to their respective area of collection. The 156 accessions was classified into four groups, such as three groups of Africa collection and one group of Asia. Results of present study can be useful in identifying diverse accessions and management of this plant resource. Moreover, the novel SSR markers developed can be utilized for various genetic analyses in this species in future.

• Korean Journal of Soil Science and Fertilizer
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• v.48 no.5
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• pp.442-450
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• 2015

This study was conducted to investigate the effect of Psy-2A-CrtI (PAC), a genetically modified (GM) rice with enhanced ${\beta}$-carotene, on the soil microbial community. The soil used to cultivate GM rice and its wild-type, Nakdong, was analyzed for population density, denaturing gradient gel electrophoresis (DGGE), and pyrosequencing. It was found that the bacterial, fungal and actinomycetes population densities of the PAC soils were within the range of those of the non-GM rice cultivar, Nakdong. The DGGE banding patterns of the GM and non-GM soils were also similar, suggesting that the bacterial community structures were stable within a given month and were unaffected by the presence of a GM plant. The pyrosequencing result showed a temporal difference in microorganism taxon and distribution ratio, but no significant difference between GM and non-GM was found. The persistence of the transgene DNA in the plant and surrounding soil were investigated for different time periods. There were differences in the persistence within the plant depending on the gene, but they could not be detected after 5 weeks. Also the transgenes were not detected in the surrounding soil. These results indicate that soil microbial communities are unaffected by the cultivation of a PAC rice within the experimental time frame.

• Journal of Environmental Science International
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• v.20 no.4
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• pp.527-540
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• 2011

The investigation was made about distribution and ecological characteristics of Rhododendron mucronulatum var. mucronulatum, R. dauricum and R. schlippenbachii on Mt. Changbai in China. The Ericaceae plants are known to be highly ornamental and easy to cultivate. The results of investigations are like followings. The plant species diversity was the highest in habitats of R. mucronulatum var. mucronulatum, followed by those of R. schlippenbachii and R. dauricum. R. mucronulatum var. mucronulatum community was found in the northwest exposure areas of 300m in altitude. Corylus heterophylla, Carex humilis var. nana, Atractylodes japonica species also grew in the R. mucronulatum var. mucronulatum community. R. dauricum communities were widely distributed in the west exposure areas of 1226-1860m above sea level. R. schlippenbachii populations were found in areas of under 300m above sea level, which has dry soil. But humidity of air was relatively high in the place. Artemisia japonica, Lespedeza bicolor and Dactylis glomerata were found around the habitats of R. schlippenbachii. Phalanx type of plants were dominant near the habitats of Ericaceae plants as 61%. Further, there appeared higher plant life totalling to 72 taxa comprising 54 species, 16 varieties and 2 forma with 31 families and 59 genuses at the whole survey area. Among these, Compositae plants excellent in resistance to environment was surveyed the most, accounting for 30.0%.

• The Plant Pathology Journal
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• v.40 no.2
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• pp.151-159
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• 2024

Bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum (Pcc) is one of the most severe diseases in radish cultivation. To control this plant disease, the most effective method has been known to cultivate resistant cultivars. Previously, we developed an efficient bioassay method for investigating resistance levels with 21 resistant and moderately resistant cultivars of radish against a strain Pcc KACC 10421. In this study, our research expanded to investigate the resistance of radish cultivars against six Pcc strains, KACC 10225, KACC 10421, ATCC 12312, ATCC 15713, LY34, and ECC 301365. To this end, the virulence of the six Pcc strains was determined based on the development of bacterial soft rot in seedlings of four susceptible radish cultivars. The results showed that the Pcc strains exhibited different virulence in the susceptible cultivars. To explore the race differentiation of Pcc strains corresponding to the resistance in radish cultivars, we investigated the occurrence of bacterial soft rot caused by the six Pcc strains on the 21 resistant and moderate resistant cultivars. Our results showed that the average values of the area under the disease progress curve were positively correlated with the virulence of the strains and the number of resistant cultivars decreased as the virulence of Pcc strains increased. Taken together, our results suggest that the resistance to Pcc of the radish cultivars commercialized in Korea is more likely affected by the virulence of Pcc strains rather than by race differentiation of Pcc.

• Journal of Plant Biotechnology
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• v.45 no.3
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• pp.196-206
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• 2018

Dramatic increase in global population accompanied by rapid industrialization in developing countries has led to serious environmental, food, energy, and health problems. The Food and Agriculture Organization of the United Nations has estimated world population will increase to 9.7 billion by 2050 and require approximately 1.7 times more food, and more than 3.5 times energy than that of today. Particularly, sweetpotato is easy to cultivate in unfavorable conditions such as heat, drought, high salt, and marginal lands. In this respect, sweetpotato is an industrially valuable starch crop. To replace crops associated with these food and energy problems, it is necessary to develop new crops with improved nutrients and productivity, that can be grown on marginal lands, including desertification areas using plant biotechnology. For this purpose, exploring useful genes and developing genetically modified crops are essential strategies. Currently, sweetpotato [Ipomoea batatas (L.) Lam.] have been re-evaluated as the best health food and industrial crop that produces starch and low molecular weight antioxidants, such as vitamin A, vitamin E, anthocyanins and carotenoids. This review will focus on the current status of research on sweetpotato biotechnology on omics including genome sequencing, transcriptome, proteomics and molecular breeding. In addition, prospects on molecular breeding of sweetpotato on marginal lands for sustainable development were described.

• Korean Journal of Plant Resources
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• v.26 no.2
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• pp.284-288
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• 2013

This study aimed to get the basic data on the breeding of good varieties in Hypericum patulum Thunberg. The optimum materials, concentration and soaking time were examined to identify the effective approach to induce tetraploid plant by colchicine treatment to cultivate the varieties. For the seed germination rate of seed by colchicine treatment, the higher colchicine concentration was and the longer soaking time was, the more the germination rate decreased. While individuals were germinated in 16 test groups except control group (no treatment group), all the plants were diploid and no tetraploid was induced. For the plant regeneration rate by colchicine treatment on the explant of Hypericum patulum Thunberg that was under in vitro culture, the higher the colchicine concentration increased, the ress the regeneration rate. While total 147 individuals were regenerated in all treatment, when the explant was soaking treatment in more than 0.05% for over 6 hours, tetraploid could be obtained. In the soaking treatment of 0.05% for over 6 hours, tetraploid could be obtained. In particular, for the soaking treatment in 0.05% for 12 hours, 8 tetraploids were induced, which was about 47.1% of the number of plant regenerated. In accordance with the observation on doubling of DNA contents in leaf in order to identify polyploidy, the peak DNA content of G1 phase was 94.5 for diploid and 192.5 for tetraploid. It confirmed doubling of DNA content. Furthermore, the number of chloroplasts per guard cell depending on polyploid was around 10 in diploid and 17 to 19 in tetraploid, which were around 1.7 to 1.9 times as much as diploid.

• Horticultural Science & Technology
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• v.33 no.6
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• pp.839-844
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• 2015

This study was carried out to investigate seedling quality, growth characteristics and yield of hot pepper (Capsicum annum) grown in the open field according to seedling growth periods (SGPs) of 45, 55, 65, 75, and 85 days. Before planting, plant height, node number, leaf area, fresh and dry weight of seedlings were high in longer-SGP treatments, the T/R ratio was high in SGP 45 and SGP 85 treatments compared with other treatments. At 10 weeks after planting, plant height, stem diameter and leaf area of plants treated with SGP 45 and SGP 55 were significantly higher compared to other treatments. Fresh and dry weight of the plant with SGP 45 treatment was greatest, however, the dry matter percentage with SGP 45 was low compared to other treatments. Fruiting number and weight per plant were highest in SGP 45 treatment. Shorter SGP treatments such as 45 and 55 days gave greatly increased total weight of ripened fruit at 18 weeks after planting. Our results showed that SGP for hot pepper grown in the open field influences plant growth parameters and marketable yield, so that SGP 45 to SGP 55 is optimum to cultivate hot pepper plant.