• 한국항만학회지
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• 제10권1호
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• pp.63-74
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• 1996

The flow analysis around the Bang-o-jin breakwater was carried out by the numerical simulation based on the package program NISA Ⅱ. The averaged Navier-Stokes equations are changed to a weak formulation, which is an application of finite element method. To get a better circulation of sea-water current, various types of breakwater modification were numerically tried for the purpose of comparisons. According to the numerical results, it proved to be very difficult to have the seawater circulate in every corner inside the breakwater. However, some cases give the promising feasibility provided that the waste water disposal plant should be additionally made.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.224-224
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• 2017

In plants, cysteine(Cys) is decisive for protein and glutathione that acts as an indispensable sulfur grantor for methionine and many other sulfur containing secondary products. Cys formation is involved in the consecutive two reactions using two enzymes-serine acetyl transferase (SAT) and O-acetylserine (thiol)lyase (OASTL) and appeared in plant cytosol, chloroplast and mitochondria. OASTL is able to produce mimosine with 3-hydroxy-4-pyridone (3H4P) in lieu of $H_2S$ for Cys. In this report, we describe the first time cloning, purification and characterization of cytosolic(cy)OASTL from M. pudica and its expression in Escherichia coli and try to find out the cross link between this OASTL and the mimosine formation and to elucidate the metabolic role of cy-OASTL in M. pudica. The purified recombinant protein was 34.7 KDa. The optimum reaction pH and temperature was 6.5 and $50^{\circ}C$, respectively. The Michaelis constant (Km) and the Vmax value of the enzyme was $252{\pm}25{\mu}M$ and $57{\pm}3{\mu}M\;cysteine\;min^{-1}\;{\mu}g\;protein^{-1}$ for sulfide and $159{\pm}21{\mu}M$ and $58{\pm}2.4{\mu}M\;cysteine\;min^{-1}\;{\mu}g\;protein^{-1}$ for OAS subsequently. After cleaving the His-tag, we tried to observe cy-OASTL to form mimosine with appropriate substrate but it was not successful. It may be concluded that cy-OASTL of the present study is only Cys specific, not mimosine.

• Journal of Applied Biological Chemistry
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• 제54권1호
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• pp.67-70
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• 2011

The roots of Glycyrrhiza uralensis Fisch. were extracted with 30% aqueous ethanol (EtOH), and the concentrated extract was partitioned with n-hexane, chloroform ($CHCl_3$), ethyl acetate (EtOAc), n-butanol (n-BuOH), and $H_2O$, successively. From the $CHCl_3$ fraction, four flavonoids were isolated through the repeated silica gel ($SiO_2$), octadecyl silica gel (ODS), and Sephadex LH-20 column chromatographies (c.c.). According to the results of spectroscopic data including nuclear magnetic resonance spectrometry (NMR), electron ionization mass spectrometry (EI/MS), and infrared spectroscopy (IR), the chemical structures of the compounds were determined as glabrol (1), abyssinone II (2), glabridin (3), and isoliquiritigenin (4). The flavonoids were evaluated for cytotoxic effect against human cancer cell lines, HCT-116, HepG2, HeLa, SK-OV-3, SK-BR-3, MCF-7, and SK-MEL-5. Especially, glabrol (1) and glabridin (2) showed $IC_{50}$ values of lower than $25{\mu}M$.

• 한국작물학회지
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• 제46권1호
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• pp.1-5
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• 2001

Field experiment was conducted during 1993-94 season to determine the pattern of nutrient uptake and productivity of maize/sweet potato intercropping system. Four levels of nitrogen (0, 50, 100 and 150kg N ${ha}_{-1}$) and four levels of potassium (0, 40, 80 and 120kg $K_2$O ${ha}_{-1}$) formed treatment variables. Plants were sampled periodically to determine dry matter and tissue concentrations of N and K in the individual plant components of intercropped maize and sweet potato. Nitrogen and potassium fertilizer did not interact significantly to nutrient uptake by any plant parts of intercropped maize and sweet potato. But application of N fertilizer independently enhanced N uptake in all the plant parts of maize and sweet potato. The uptake of N in leaf, leaf sheath, stem, husk, and cob of maize increased upto 90 days after planting (DAP) but grain continued to accumulate N till its maturity. Sweet potato exhibited a wide variation in N uptake pattern. Sweet potato leaf shared the maximum uptake of N at 50 DAP which rapidly increased at 70 DAP and then declined. Declination of N uptake by petiole and stem were observed after 120 DAP whereas N uptake by tuber increased slowly upto 90 DAP and then rapidly till harvest. Rate of applied K had very little effect on the uptake patterns in different components of intercropped maize. Pattern of K uptake by leaf, petiole and stem of sweet potato showed almost similar trend to N uptake. But uptake of K by tuber increased almost linearly with the K application. Pattern of N and K uptake by grain and tuber paralleled the grain yield of maize and sweet potato respectively. Intercropped productivity of maize and sweet potato found to be better by the application of 100kg N and 120 kg $K_2$O ${ha}_{-1}$

• 공업화학
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• 제32권6호
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• pp.659-663
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• 2021

반도체 제조공정에서 발생하는 미세먼지와 질소산화물 동시처리를 위하여 오존산화, 습식중화 및 습식전기집진 기술들을 직접화한 10 CMM급 복합오염물질 제거시스템을 개발하였으며, NOx 제거효율 증대를 위한 공정변수 제어 및 최적화를 진행하였다. 특히, 전원공급장치를 포함한 습식전기집진장치 핵심부품 안정성 평가를 위해 30일 동안의 장기운전도 병행하였다. 오존산화 기반 DeNOx 공정에서 가장 중요한 운전변수인 O₃/NO 비율은 1.5 부근에서 최적화하였으며, 습식중화 공정의 운전변수를 최적화하여 중화반응에 의한 제거효율 기여도를 확인하였다.

• Journal of Plant Biotechnology
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• 제35권4호
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• pp.307-316
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• 2008

식물에서 전이인자를 이용한 삽입 변이체의 유전자 기능분석 연구가 최근 가장 활발하게 이루어지고 있다. 본 연구에서는 동진벼의 Ac/Ds 삽입 변이체인 F2 세대 30,000 계통을 이용하여 고염과 저온에 민감한 계통과 내성이 있는 계통을 대량 스크리닝을 통해 선발하였다. 첫 번째 스크리닝에서 선발한 212 계통을 Southern blot 분석을 통해 Ds의 삽입여부 및 copy 수를 꽉인하고 표현형과 비교하여 고염과 저온에서 총 19 계통을 선발하였고, 이 중 copy 수가 하나인 계통은 13 계통이었다. 선발한 계통을 FSTs 분석을 통해 Ds의 삽입위치 및 knock-out유전자를 확인하고 염기서열 정보를 이용하여 벼 전체 염기서열 정보와 상동성 비교분석 결과 세포의 신호전달 과정과 조절 관여하는 유전자 그룹인 transpoter, protease family protein and apical meristem family protein, 삼투압조절에 관여하는 유전자 그룹인 heat shock potein, O-methyltransferase, glyceraldehyde-3-phosphate dehydrogenase and drought stress Induce protein 그리고 식물의 소포유통(vesicle trafficking)에 관여하는 유전자 SYP 5 family protein로 구분할 수 있었다. 선발된 19개 유전자의 발현 분석을 위해 9종류 비생물학적 스트레스 하에서 RT-PCR을 수행한 결과 이들 knock-out 유전자는 비생물학적 스트레스에 각각 다른 발현 패턴을 보였다. 이 연구의 결과는 삽입 변이체를 통한 유전자의 기능분석에 있어서 비생물학적인 스트레스의 응답 반응계에 관여하는 유전자를 연구하는데 유용할 것이라고 생각된다.

• 한국CDE학회논문집
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• 제21권2호
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• pp.111-121
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• 2016

The plant equipment usually has a long life cycle. During its O&M (Operation & Maintenance) phase, since the occurrence of an accident of offshore plant equipment causes catastrophic damage, it is necessary to make more efforts for managing critical offshore equipment. Nowadays due to the emerging ICTs (Information Communication Technologies) and sensor technologies, it is possible to gather the health status data of important offshore equipment and their environment data, which leads to much concern on CBM (Condition-Based Maintenance). In this study, we will propose an approach to estimate the remaining lifetime of an offshore plant equipment (pump tower) based on gathered ocean environment data.

• 한국CDE학회논문집
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• 제19권3호
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• pp.203-213
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• 2014

The O&M (Operation and Maintenance) phase of offshore plants with a long life cycle requires heavy charges and more efforts than the construction phase, and the occurrence of an accident of an offshore plant causes catastrophic damage. So previous studies have focused on design for reliability, and recently many studies have dealt with a maintenance system to prevent unexpected failures. Nowadays due to the emerging ICTs (Information Communication Technologies) and sensor technologies, it is possible to send health monitoring information of important equipment to administrator of an offshore plant in real time, which leads to having much concern on condition based maintenance policy or predictive maintenance. In this study, we have reviewed previous studies associated with condition-based maintenance of offshore plants, and introduced the approaches predicting failures of the compressor which is one of essential mechanical devices in LNG FPSO.

• 산업경영시스템학회지
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• 제37권4호
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• pp.12-23
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• 2014

The O&M (Operation and Maintenance) phase of offshore plants with a long life cycle requires heavy charges and more efforts than the construction phase, and the occurrence of an accident of an offshore plant causes catastrophic damage. So previous studies have focused on the development of advanced maintenance system to avoid unexpected failures. Nowadays due to the emerging ICTs (Information Communication Technologies) and sensor technologies, it is possible to gather the status data of equipment and send health monitoring data to administrator of an offshore plant in a real time way, which leads to having much concern on the condition based maintenance policy. In this study, we have reviewed previous studies associated with CBM (Condition-Based Maintenance) of offshore plants, and introduced an algorithm predicting the next failure time of the compressor which is one of essential mechanical devices in LNG FPSO (Liquefied Natural Gas Floating Production Storage and Offloading vessel). To develop the algorithm, continuous time Markov model is applied based on gathered vibration data.

• 식물조직배양학회지
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• 제22권4호
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• pp.195-200
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• 1995

동물유전자인 mouse adenosine deaminase (ADA)유전자가 안정적으로 연초 형질전환체에서 발현되었다. ADA cDNA 을 연초에서 강력히 발현시키기 위해서 35S/35S/AMV promoter을 부착시켰으며 식물세포에 도입을 위해서 binary vector인 pRD400을 이용하였다. 연초의 형질전환은 Tri- parental mating에 의해서 도입된 ADA 유전자 함유 binary vector와 disarmed Ti-plasmid을 함유하고 있는 Agrobacterium tmefacience MP9O을 사용하였다. 동시배양은 연초의 잎 disc을 이용해서 kanamycin이 첨가된 배지로부터 직접 shoots을 선발하여 형질전환체로 유도하였다. 형질전환 체에 ADA 유전자의 삽입여부는 PCR을 이용하였으며, 실험결과 ADA 유전자를 확인할 수 있었다. 또한 도입된 mouse ADA 유전자로부터 mRNA 및 단백질 합성여부를 각각 northern blot 및 immunoblot 분석한 결과 형질전환체 에서는 공히 확인되었다.