• 제목/요약/키워드: Plant Leaf Disease

검색결과 779건 처리시간 0.025초

벼 누른오갈병(Sclerophthora macrospora) 발생이 벼 생육에 미치는 영향 및 병 저항성 품종 검정 (Effect of Rice Downy Mildew (Sclerophthora macrospora) on Rice Growth and Screening of Disease Resistance of Cultivars)

  • 이용환;차광홍;고숙주;박기범;김영철
    • 식물병연구
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    • 제9권1호
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    • pp.52-56
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    • 2003
  • 전남 나주의 벼 누른오갈병이 발생한 담수직파 포장에서 일미벼를 대상으로 병 발생이 벼 생육에 미치는 영향을 조사하고 품종저항성 검정을 수행하였다. 누른오갈병 발생시 잎에는 퇴록반점이 나타나고 엽장이 짧아지며 벼의 생육이 진행되면서 줄기의 절간신장이 제대로 이루어지지 않아 마디사이가 기형적으로 자라고 출수가 안되거나 기형이삭이 되는 증상을 보였다. 벼 누른오갈병 이병시 초장이 짧아졌고, 분얼수는 최고분얼기 전에 발병주가 적었지만 그 이후에는 발병주가 많았다. 마디수는 발병줄기가 건전줄기에 비해 많은 반면에 마디길이는 짧았다. 발병정도가 심할 수록 단위면적당 이삭수가 적고 등숙율이 낮아 수량이 크게 감소하였다. 유묘를 대상으로 품종 저항성 검정을 실시한 결과, 동진벼, 계화벼가 벼 누른오갈병에 대해서 저항성이었다.

Response of Mulberry Brown Leaf Spot Fungus Myrothecium roridum to Different Plant Extracts

  • Chattopadhyay, S.;Institute, Traning;Majil, M.D.;Pratheesshkumar;Das, K.K.;Saratchandra, B.
    • International Journal of Industrial Entomology and Biomaterials
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    • 제5권2호
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    • pp.183-188
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    • 2002
  • Anti-fungal potential of 5 plant extracts viz., Eucalyptus citriodora, Allium sativum, Cassia sophera, Chromolaena odorata and Datura metel on the growth of mulberry brown leaf spot pathogen Myrothecium roridum were examined. Except fur the aqueous extract of Allium bulb, ethanolic leaf extract of all other plants more efficiently reduced the colony growth of the fungus on potato-dextrose-agar, Of which, Allium and Eucalyptus extracts were more effective. Initiation of radial growth of M. roridum on solid media was deferred maximum 6 days by ethanolic Eucalyptus extract and 4 days by aqueous Allium extract at $0.4 mg.ml^{-1}$. In the liquid media amended with Eucalyptus extract ($0.4 mg.ml^{-1}$) complete inhibition of sporulation was noticed upto 8 days, and initial inhibition of mycelial bio-mass generation was considerably diminished with time and reduction was 1.3 fold 14 days after application. While, complete inhibition of mycelial growth for 6-14 days was recorded with $\geq$0.1 mg.ml$^{-1}$ commercial eucalyptus oil. However, rejuvenation of growth appeared when fungus was re-inoculated in fresh media. Post-inoculate application of different doses Of Eucalyptus and Allium extracts significantly (p < 0.05) reduced the disease severity in pot-ted mulberry. However, persistence of the effect up to 28 days was apparent at $\geq$ 1.0 mg.ml$^{-1}$ and effectively was on par with carbendazim (1 mg.ml$^{-1}$ ). Almost equal control ability of 1.0 mg.ml$^{-1}$ Eucalyptus extracts can be achieved by ca. 10 times lowered dose of commercial eucalyptus oil. It seems, the toxic principle of E. citrodora to M. roridum is fungistatic in nature and may have essential oil based origin.

Evaluation of Potential of Mandarin Hybrid 'Shiranuhi' against inoculation of Bacterial Canker Disease Pathogen (Xanthomonas axonopodis pv. citri) in Citrus Field in Jeju Island

  • Hyun, Jae-Wook;Myung, Inn-Shik;Lee, Seong-Chan;Kim, Kwang-Sik;Lim, Han-Cheol
    • The Plant Pathology Journal
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    • 제19권5호
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    • pp.248-252
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    • 2003
  • This study was carried out to evaluate potential of Shiranuhi cultivar against inoculation of causal pathogen of citrus canker, Xanthomonas axonopodis pv. citri in Jeju Island by comparing degree of susceptibility of fruits and leaves/twigs, and analysis of incidence of canker disease. Progression of symptom, disease incidence, and percent area of lesion were surveyed for evaluation of resistance. In greenhouse condition, symptoms of bacterial citrus canker progressed more rapidly in sweet orange, a moderately susceptible cultivar, than in other four cultivars (satsuma mandarin, 'Kiyomi', 'Shiranuhi' and 'Yuzu'). At 20 days after inoculation, disease severity was the highest in sweet orange (5.0$\pm$0.0), and all tested leaves were distorted or had dropped. On the other hand, 'Yuzu' showed the lowest disease severity (2.6$\pm$0.47), followed by 'Kiyomi' (4.0$\pm$0.0), 'Shiranuhi' (4.0$\pm$0.82), and satsuma mandarin (4.3$\pm$0.47). Percent area of lesion per leaf 30 days after inoculation was the highest in sweet orange (8.31$\pm$1.78), followed by satsuma mandarin (1.51$\pm$1.25), 'Shiranuhi' (1.39$\pm$0.94), and 'Kiyomi' (1.1$\pm$0.9), while the lowest was in 'Yuzu' (0.26$\pm$0.17). Infield condition, percentage of diseased leaf in 'Shiranuhi' was very low, 5.2$\pm$2.9, compared with sweet orange, 71.0$\pm$ 11.5, while that of satsuma mandarin and 'Kiyomi' were 6.9$\pm$7.0 and 4.3$\pm$2.0, respectively. Percentages of diseased leaf was higher (17.4$\pm$7.1) than that of diseased fruit (3.2$\pm$2.5) in severely diseased trees of Shiranuhi cultivar, and the disease was not observed on twig in open field condition. Lesion sizes on leaves and fruits in open field condition were 4.1$\pm$2.2 mm2 and 5.1$\pm$5.6 mm2, respectively, while those in greenhouse condition were 8.7$\pm$5.7 mm2, 10.4$\pm$9.2 mm2 and 5.6$\pm$2.6 mm2 on leaves, fruits and twigs, respectively. The disease was observed in 5.6% out of total 107 farmers Shiranuhi fields under polyethylene film house, and average percentages of diseased tree in 31 fields of Shiranuhi cultivar and adjacent satsuma mandarin fields were 0.02% and 14.8%, respectively. Average percentage of diseased fruit was 1.6% in satsuma mandarin which was not observed in anyone of all the 31 Shiranuhi farmers fields. Therefore, it was concluded that 'Shiranuhi' cultivar is not potential against causal pathogen of citrus canker disease in Jeju Island because the cultivar has similar resistance as satsuma mandarin which occupies over 95% of total 25,000 ha in Jeju Island in polyethylene film houses protected from outside.

Phytophtora capsici에 의한 수박 역병 (Fruit and Vine Rot of Watermelon Caused by Phytophthora capsici)

  • 김병수
    • 한국식물병리학회지
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    • 제11권1호
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    • pp.98-99
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    • 1995
  • A disease causing fruit rot and leaf and vine blight on watermelon was found in Euseong in July, 1991 and in Bonghwa, Kyungpook province in August, 1993. Abundant zoosporangia characteristics of Phytophthora capsici were formed on the surface of the infected fruits. The zoosporangia were ovoid to ellipsoid, tapering to the base and with conspicuous papilla. The fungus isolated from the infected fruits was pathogenic on seedlings of watermelon, pumpkin, and pepper. The morphological characteristics and host range agreed with those of P. capsici. The watermelon disease caused by P. capsici was reported long time ago in Japan and America but rot yet in Korea. Thus, the disease on watermelon caused by P. capsici is reported as a new record in Korea.

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매자기에 갈색무늬병(가칭)을 일으키는 Nimbya scirpicola (Nimbya scirpicola Causing Brown Spot of Bayonet-Gras (Scirpus maritimus))

  • 유승헌;윤해근;심형권
    • 한국식물병리학회지
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    • 제10권1호
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    • pp.61-63
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    • 1994
  • A brown leaf and stem spot disease of bayonet-grass (Scirpus maritimus) was epidemic in reclaimed paddy fields of Chunbuk province, Korea. A fungal pathogen was repeatedly isolated from the necrotic lesions of the bayonet-grass and identified as Nimbya scirpicola. The pathogen induced disease symptoms only in bayonet-grass but not in 8 other plants tested; Brassica pathogen induced disease symptoms only in bayonet-grass but not in 8 other plants tested; Brassica compestris subsp. napus var. pekinensis, Cucumis sativus, Glycine max, Hordeum vulgare, Lycopersicon esculentum, Oryza sativa, Sesamum indicum and Triticum aestivum. The fungus has potential to be developed as a mycoherbicide.

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고추 흰가루병 저항성 평가방법 개발 (Development of Resistance Evaluation Method for Powdery Mildew (Leveillula taurica) in Capsicum spp.)

  • 김수;김동휘;김대현;한경숙;한유경;이성찬;조명철;양은영;김기홍
    • 식물병연구
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    • 제17권3호
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    • pp.311-316
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    • 2011
  • 고추 흰가루병은 시설재배의 증가로 경제적인 피해가 증가하는 병해이다. 본 연구에서는 고추 유전자원의 흰가루병 저항성 평가를 위한 새로운 저항성 평가 방법을 개발하였다. S index라고 명명한 평가방법의 공식은 다음과 같다; (최상위 발병 엽수/정단부 엽수) - (최하위 발병 엽수/정단부 엽수). 흰가루병에 의해 발병된 고추 식물체에서 이병엽율과 S index는 81%(P = 0.01)의 정의 상관으로 고도의 유의성을 보였다. 그리고 34종의 유전자원에서 저항성을 평가한 결과, S index는 흰가루병 저항성 계통을 구분할 수 있는 것으로 확인되었다. 이들 중 Capsicum frutescens 인 3CA131과 C. baccatum인 3CA0162, 3CA174, 3CA176은 흰가루병에 강한 저항성으로 평가되었다. 이 결과는 고추 흰가루병 저항성품종 육성에서 Sindex의 유용성을 증명한 결과이다.

포도 캠벨얼리의 무가온 하우스재배시 지역별 갈색무늬병 발생차이 (Regional Differences of Leaf Spot Disease on Grapevine cv. 'Campbell Early' Caused by Pseudocercospora vitis in Plastic Green House)

  • 정성민;박종한;박서준;이한찬;이재욱;류명상
    • 식물병연구
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    • 제15권3호
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    • pp.193-197
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    • 2009
  • 포도갈색무늬병은 주로 포도 캠벨얼리에서 6월 상순에 발생이 시작하여 장마철을 지나면서 급속히 전파되는 중요한 병중 하나이다. Pseudocercospora vitis에 의해 발생되는 갈색무늬병의 발생소장을 김제, 김천에 소재한 무가온 하우스에서 2007년에 조사하였다. 병의 발생율은 노지, 무가온 하우스에 따라 매우 다르게 나타났고 지역간의 차이는 나타나지 않았다. 무가온 하우스내의 온도와 상대습도와 같은 미기상요인을 대상으로 갈색무늬병 이 병엽률과의 연관성을 SAS의 PROC REG방법을 사용하여 통계분석 하였다. 그 결과 미기상 요인과 병발생간에 Y(Infected leaves)=-7.0101+0.0496$\times$20Hcum+0.0208$\times$20cum-0.2781$\times$25Hcum ($R^2=0.95^{**}$)과 같은 회귀식이 도출되었고 이는 특정 이상 온도 및 시간의 누적값이 무가온하우스 작형에서 Pseudocercospora vitis에 의한 포도 갈색무늬병에 있어 중요인자임을 보여준다.

Pseudomonas syringae pv. syringae에 의한 애호박 세균점무늬병 (Bacterial Spot Disease of Green Pumpkin by Pseudomonas syringae pv. syringae)

  • 박경수;김영탁;김혜성;이지혜;이혁인;차재순
    • 식물병연구
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    • 제22권3호
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    • pp.158-167
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    • 2016
  • 육묘장과 재배포장의 애호박에서 새롭게 발생한 병원균의 특성을 분석하여 동정하였다. 병징은 잎에 수침상의 병반과 강한 노란색의 둘레무리를 가진 점무늬, 꽃에서 갈색 병반, 열매에 노란색의 점무늬였다. 잎의 점무늬로부터 분리된 세균은 박과작물 8개의 식물체, 흑종호박, 애호박, 쥬키니호박, 풋호박, 참박, 참외, 멜론, 오이에 병원성이 있었지만 수박과 밤호박에서는 병을 일으키지 못했다. 분리세균은 다발모를 가진 막대형으로 그람음성이며 King's B 배지에서 형광성이었으며, LOPAT 1a 그룹에 속했다. 그들의 Biolog 기질이용성은 Biolog database의 P. syringae pv. syringae 이용성과 유사하였다. 16S rRNA 유전자 염기서열을 이용한 계통수와 4개의 항존유전자(gapA, gltA, gyrB, rpoD)의 염기서열과 PAMDB에 있는 P. syringae균주들의 염기서열을 이용한 MLST는 애호박 분리균주들이 P. syringae pv. syringae 균주들과 동일한 그룹(clade)으로 그룹화되었고, MLST 계통수에서 애호박 분리균주들의 그룹(clade)은 genomospecies 1에 속하였다. 표현형적, 유전적 특성은 애호박 분리균이 P. syringae pv. syringae임을 제시하였다.

Introduction of Hog Cholera Virus Gene into Potato Plants by Agrobacterium-mediated Transformation and the Analysis of Its Expression

  • Kim, Hyun-Soon;Jeon, Jae-Heung;Kim, Cheol-Jung;Hyouk Joung
    • Journal of Plant Biotechnology
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    • 제4권4호
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    • pp.155-161
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    • 2002
  • The HCV gene was expressed in potato plants under the control of the constitutive CaMV 355 promoter or tuber-specific patatin promoter. Solanum tuberosum plants carrying a plant expression vector harboring the encoding region of HCV gene were generated by Agrobacterium tumefaciens-mediated in vitro transformation methods. The presence of HCV gene in the plant genome was detected by PCR and DNA hybridization experiments. We obtained the 5 lines of transgenic potato with the pMBPHCV construct and 4 lines of transgenic potato with the pATHCV construct. The HCV transgenic stably integrated into the potato genome, as well as their transcription. HCV mRNA was identified in leaf and tuber tissues of transgenic plants by Northern blot analysis. The transgenic potato plants produced the expected transcript, and the corresponding HCV protein accumulated in individual transgenic plants.