• Archives of Pharmacal Research
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• v.16 no.3
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• pp.244-250
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• 1993

Calli and suspension cultures were obtained following inoculation of the explant from leaves of Ginkgo biloba L on the supplemented MS basal medium. The obtained calli and suspension cultured cells were able to produce detectable amounts of ginkgolides which are known as natural specific PAF antagonists. The production of ginkgolides in the calli and suspension cultured celles were identified using GC/MS, GC and HPLC with authentic ocmpounds. Since the production of ginkgolides A and B the calli and suspension cultured cells had been confirmed, effects of types and concentration of plant growth regulators, media and illumination on the induction and growth of the callus were studied. The concentrations of growth regulators for optimal callus were studied. The concentrations of growth regulators for optimal callus induction were studied. The concentrations of growth regulators for optimal callus induction were 1.0 to 2.0 mg/L for NAA and o.1 mg/L for kinetin. The growth of the Callus seemed to be more simnultaed with the combination of NAA and kinetin than NAA and BA with illumination at all concentration ranges of 1.0 to 4.0 mg/l for NAA and o.1 to 1.0 mg/L for kinetin or BA studied. Amogn 8 different media used, the induction rate of callus on Anderson, Eriksson, and Shenk and Hildebrant at 4 weeks after the innoculation was almost the same as that of MS. However, callus was rarely induced on Heller or White medium. Suspension cultures were easily initiated with 3 g of callus (fresh weight) derived from ginkgo leaves on supplemented MS medium. A typical growth curve of suspension cultured cells could be obtained by measuring the fresh weight of the suspension cultured cells at every 3 days. To improve the growth of suspension cultured cells of ginkgo, effects of concentrations of NAA, sucrose, phosphate ions and molar ratio of $NH_{4}^+\;to\;NO_{3}^-$ ions in the culture medium were studied. The maximum growth of the cells was achieved when the culture medium contained 1.0 mg/L of NAA, 30 g/L sucrose, 1.75 mM phosphate ions and 1:5 molar ratio of $NH_{4}\;to\;NO_{3}^-$ ions.

• Journal of Bio-Environment Control
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• v.31 no.2
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• pp.133-141
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• 2022

Bulbophyllum auricomum Lindl. is a rare orchid and has flowers with an attractive fragrance. The present study investigated the tissue culture method for micropropagation. Capsules derived from artificial self-pollination were obtained for the best seed germination in MS basal medium. Plant growth regulators (1.0 mg·L^-1 of BAP and 2.0 mg·L^-1 of NAA) were affected by callus induction from subcultured pseudobulb explants. For the callus subculture, different natural plant extracts were tested in 11 treatment media. Among them, MS medium with 150 mL·L^-1 of coconut water was generally effective in fresh weight (1.75 ± 0.08) and (3.01 ± 0.20) of callus proliferation and PLBs induction at 1 and 2 months, respectively, followed by an MS combination of 30 g·L^-1 of banana and 20 g·L^-1 of potato extract. The results of a comparative study of different MS mediums containing plant growth regulators with a natural extract combination and MS medium supplemented with natural extract only showed that MS medium supplemented with a combination of natural extracts (150 mL·L^-1 of coconut water) and plant growth regulators (2.0 mg·L^-1 of BAP and 1.0 mg·L^-1 of NAA) obtained the highest shoot regeneration (3.37 ± 0.17) and (6.41 ± 0.68) after 1 month and 2 months of culturing, respectively.

• Korean Journal of Medicinal Crop Science
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• v.12 no.6
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• pp.508-514
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• 2004

Chrysanthemum boreale M. is an important medicinal plant that has been historically used in herbal medicine and in the health food throughout East Asia. This study was conducted to investigate the influence of abscisic acid (ABA) and salicylic acid (SA) on plant growth, mineral content and effective components, such as essential oil, amino acid and cumambrin A, by means in order to increase the productivity and the quality of flowerheads in the plant. Yields of flowerheads were increased by 12.7%, 21.7% and 15.5% by ABA, SA and both treatments, respectively, as compared with the control. Inorganic nutrient content was changed by PGRs; SA treatment was increased by nitrogen, phosphorus and magnesium content but decreased by potassium of C. boreale M. flowerheads. Total content of amino acid was increased by SA but decreased by ABA treatment. Essential oil content and yields were increased to 9.7% and 33.8% by SA treatment. Moreover, the content of terpene, monoterpenoids and sesquiterpenoids, were improved by ABA treatment, especially, germacrene-D content was increased by 39.1%, as compared to control. In addition, yields of cumambrin A, sesquiterpene compound exhibiting blood-pressure activity, increased in all PGRs treatments, but its concentration in the C. boreale M. flowerheads only increased by ABA and both treatment. The experiment suggests that PGRs using ABA and SA could increase the yields and quality of C. boreale M. flowerheads.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.2
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• pp.141-148
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• 1992

Field experiment was carried out to investigate the effect of plant growth regulator (PGR); IAA, GA, Kinetin to regrowth of sorghum and pearlmillet according to variety and plant growth stage. Kinetin application after cut increased tiller number and decreased dry weight of regrowth, but its application on sorghum stubble in water stress increased tiller number and leaf elongation rate, consequently increased regrowth dry weight. GA application reduced tiller production in both species, but tiller formation in pearlmillet was decreased more than in sorghum by promoting leaf elongation of old tiller. Nonstructural carbohydrate (NSC) of stubble during regrowth was consumed less at anthesis than at stem elongation stage because of senescence of tiller primordia. GA treatment reduced NSC content more than other PGR in both plant species, by consuming reserve NSC and stimulating rapid elongation of old tiller after cut. Dry matter increase during regrowth had high correlation with tiller number and tiller elongation a week after cut, while it did not have any correlation with NSC at cutting stage or with consumption of NSC during regrowth. Therefore, regrowth in sorghum and pearmillet must depend upon activity of tiller primordia more than upon amount of reserved NSC.

• BMB Reports
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• v.42 no.7
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• pp.439-443
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• 2009

Dehydrins are group II, late embryogenesis abundant proteins that act putatively as chaperones in stressed plants. To elucidate the function of dehydrins in poplar, we isolated the $SK_2$-type dehydrin gene Podhn from Populus alba $\times$ P. tremula var. glandulosa suspension cells and analyzed its expression following treatments of abiotic stress, wounding and plant growth regulator. Sequence homology and phylogenetic analyses indicate Podhn encodes an acidic dehydrin (pI 5.14, 277 amino acids, predicted size 25.6 kDa) containing two lysine-rich "K-segments" and a 7-serine residue "S-segment", both characteristic of $SK_2$-type dehydrins. Southern blots show Podhn genes form a small gene family in poplar. Podhn was expressed in all tissues examined under unstressed conditions, but most strongly in cell suspensions (especially in the stationary phase). Drought, salt, cold and exogenous abscisic acid (ABA) treatments enhanced Podhn expression, while wounding and jasmonic acid caused its reduction. Therefore, Podhn might be involved in ABA or stress response.

• Horticultural Science & Technology
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• v.31 no.2
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• pp.135-140
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• 2013

This experiment was conducted to find the effects of a $GA_3$ and thidiazuron (TDZ) on seedless rate, harvest time, fruit cracking and fruit quality in 'Kyoho' grapes over two years from 2008 to 2009. In 2008, fruit clusters were dip treated with $GA_3$ $25.0mg{\cdot}L^{-1}$ twice at full bloom (FB) and 14 days after full bloom (DAFB) in a combination with TDZ 0 or $2.5mg{\cdot}L^{-1}$. Berry seedless rate and berry enlargement were slightly improved only when TDZ was added to the second $GA_3$ treatment at 14 DAFB, compared to $GA_3$ + TDZ treatments at both FB and 14 DAFB. However, berry cracking rate was significantly increased by any plant growth regulator (PGR) treatments compared to non treatment. In 2009, $GA_3$ at $12.5mg{\cdot}L^{-1}$ and $25.0mg{\cdot}L^{-1}$ was dip treated twice at FB and 14 DAFB while TDZ $2.5mg{\cdot}L^{-1}$ was treated only at 14 DAFB. Berry cracking rate was depended on the concentration of $GA_3$ applied. The higher concentration at $25.0mg{\cdot}L^{-1}$ significantly increased berry cracking rate while the lower concentration at $12.5mg{\cdot}L^{-1}$ had no effect. Also, the addition of TDZ to $GA_3$ $25.0mg{\cdot}L^{-1}$ at 14 DAFB, substantially decreased the cracking rate to the level of untreated control. Although all PGR treatments advanced fruit maturity, the most significant advance occurred when TDZ was added to $GA_3$ $12.5mg{\cdot}L^{-1}$ only at the second dip. Considering the overall aspects related to fruit maturity and quality, we concluded that the double applications of $12.5mg{\cdot}L^{-1}$ $GA_3$ at FB and 14 DAFB with addition of $2.5mg{\cdot}L^{-1}$ TDZ only at 14 DAFB was appropriate to produce about 400-500 g size of seedless 'Kyoho' grape cluster having 35-40 berries.

• Journal of Bio-Environment Control
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• v.28 no.2
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• pp.178-184
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• 2019

Strawberry ($Fragaria{\times}ananassa$) is one of the most important and popular fruit crops in the world, and 'Sulhyang' is one of the principal cultivars cultivated in the Republic of Korea for the domestic market. The growth and flower induction in strawberry is the process which influences directly on fruit bearing and yield of this crop. In this study, effect of benzyladenine (BA), gibberellic acid ($GA_3$), and salicylic acid (SA) on growth and flower bud induction in strawberry 'Sulhyang' was investigated. The 3-week-old runner plants, grown in 21-cell propagation trays, were potted and cultivated in growth chambers with $25^{\circ}C/15^{\circ}C$ (day/night) temperatures, 70% relative humidity (RH), and light intensity of $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ photosynthetic photon flux density (PPFD) provided by white light emitting diodes (LEDs). The runner plants were treated with one of three concentrations, 0 (control), 100, and $200mg{\cdot}L^{-1}$ of BA, $GA_3$, or SA solution. The chemicals were sprayed two times on leaves of runner plants at an interval of two weeks. After 9 weeks the results showed that the application of all chemicals caused reduction of root length and chlorophyll (SPAD) content as compared to the control. The lowest chlorophyll (SPAD) content was recorded in plants treated with $GA_3$. However, the treatment of $200mg{\cdot}L^{-1}$ $GA_3$ promoted leaf area, leaf fresh weight, and plant fresh weight. The greatest flower induction (85%) and number of inflorescences (4.3 inflorescences per plant) were observed in the treatment of $200mg{\cdot}L^{-1}\;SA$, followed by $100mg{\cdot}L^{-1}\;SA$. Overall, results suggest that foliar application of $GA_3$ solution could accelerate plant growth, while foliar application of SA solution could induce hastened flowering. Further studies may be needed to find out the relationship between $GA_3$ and SA solutions treated in a combination, and the molecular mechanism involved in those responses observed.

• Horticultural Science & Technology
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• v.18 no.1
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• pp.28-32
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• 2000

This study was carried out to investigate the effects of uniconazole treatment on the growth and flowering of potted Chrysanthemum indicum L. for high quality pot plant production. Uniconazole was drenched at 0.05, 0.01, or 0.15 mg a.i./pot at 14 days after planting (DAP) of rooted cuttings. Simultaneously the short-day treatment (SDT) and pinching were adapted. The same amount of uniconazole (0.05 mg a.i./pot) was spilt drenched at once, twice, and three times, respectively, at 1 week interval. Uniconazole markedly reduced plant height, branch length, and stem diameter. Plant height was reduced linearly with increasing uniconazole concentration at 0.05, 0.01, or 0.15 mg a.i./pot up-to 41.6%, 52.5%, and 58.5%, respectively. In 0.05 mg a.i./pot, the number of branches greatly increased and plant height of 22.6 cm was adequate for pot plant. However, higher concentrations (0.10, 0.15 mg a.i.) were not suitable for production of high quality pot plant (17.0, 14.8 cm, respectively). Pinching and SDT decreased the number of days to visible bud, while uniconazole treatments delayed days to visible bud by 5-9 days compared with pinching and SDT. Number of visible buds was highest at 0.05 mg a.i./pot uniconazole treatment. However, flower diameter was decreased by uniconazole treatment, resulting in compact form. Number of stomata was increased by uniconazole treatment. The length of vascular tissues of uniconazole-treated plants ($11.2{\mu}m$) was smaller than that of non-treated plants ($15.0{\mu}m$, and the size of xylem vessel was also decreased. Uniconazole treatment at 0.05 mg a.i./pot at 14 DAP with pinching and SDT were recommended for pot plant production of C. indicum L.

• Korean Journal of Weed Science
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• v.9 no.2
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• pp.97-102
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• 1989

1. IAA showed no significant growth of root primordia and then lateral root emerged at 2 days after IAA treatment. BA treatment, however, strongly inhibited the formation of root primordia and a few lateral roots, if any, emerged about 5 days after treatment. 2. Treatment of BA and Indol-B on the water retaining ability sampled 1, 3, 5 days after chemical treatment was apparent on the soybean sprouts sampled 5 days after treatment while the difference among the treatments was negligible when sampled 1 and 3 days after treatment. 3. BA stimulated ABA content in the hypocotyl while inhibited ABA content in the root of soybean sprouts. ABA may relate with water retaining ability. 4. Soaking the soybean seeds to several seed disinfectant chemical solution had no effect on the growth and elongation of soybean sprouts. 5. It can be recommended that container of soybean sprouts should be shut tight during growing period except irrigation because the ethylene accumulation in the container may stimulate hypocotyl swelling and inhibit length of soybean sprouts.

• Korean Journal of Environmental Agriculture
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• v.32 no.3
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• pp.214-223
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• 2013

BACKGROUND: This study focused on the development of an analytical method about dichlorprop (DCPP; 2-(2,4-dichlorophenoxy)propionic acid) which is a plant growth regulator, a synthetic auxin for agricultural commodities. DCPP prevents falling of fruits during their growth periods. However, the overdose of DCPP caused the unwanted maturing time and reduce the safe storage period. If we take fruits with exceeding maximum residue limits, it could be harmful. Therefore, this study presented the analytical method of DCPP in agricultural commodities for the nation-wide pesticide residues monitoring program of the Ministry of Food and Drug Safety. METHODS AND RESULTS: We adopted the analytical method for DCPP in agricultural commodities by gas chromatograph in cooperated with Electron Capture Detector(ECD). Sample extraction and purification by ion-associated partition method were applied, then quantitation was done by GC/ECD with DB-17, a moderate polarity column under the temperature-rising condition with nitrogen as a carrier gas and split-less mode. Standard calibration curve presented linearity with the correlation coefficient ($r^2$) > 0.9998, analysed from 0.1 to 2.0 mg/L concentration. Limit of quantitation in agricultural commodities represents 0.05 mg/kg, and average recoveries ranged from 78.8 to 102.2%. The repeatability of measurements expressed as coefficient of variation (CV %) was less than 9.5% in 0.05, 0.10, and 0.50 mg/kg. CONCLUSION(S): Our newly improved analytical method for DCPP residues in agricultural commodities was applicable to the nation-wide pesticide residues monitoring program with the acceptable level of sensitivity, repeatability and reproducibility.