• Korean Journal of Environmental Agriculture
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• v.25 no.1
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• pp.85-92
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• 2006

Fungicides including keroxym-methyl (strobilurin fungicide), fenarimol and trifulmizole (DMI, boscalid (anilide) and metrafenone (benzophenone) were evaluated for preventive and curative efficacies against powdery mildew on oriental melon. The fungicides were applied preventatively at 7 days, 3 days and 3 hours before inoculation. Curative applications were made by applying fungicides three times at the interval of one week when infected leaves by powdery mildew were about 16% or 30%. Then preventative and curative efficacies were evaluated one week after inoculation and final application, respectively. Boscalid and metrafenone showed preventive efficacies of 74.3 and 68.3% against powdery mildew development respectively, when treated at 7 days before inoculation. They showed 89.4 and 87.2% efficacies when treated at 3 days before iniculation and 98.5 and 93.2% at 3 hours before inoculation respectively. However, there were no preventive efficacies in kresoxym-methyl, triflumizole and femarimol (DMI fungicides) when trebled at both 7 and 3 days before inoculation. At the preventive treatment 3 hours before inoculation, they indicated 59.0, 42.5, and 37.2% efficacies, respectively. When leaves were infected about 30% by powdery mildew, curative application of boscalid and metrafenone showed 85.9 and 74.0% of efficacies, respectively. Curative efficacies of kresoxym-methyl, tiflumizole and fernarimol were 5.5, 23.0 and 46.7% respectively. When leaves were infected about 16%, curative application of boscalid and metrafenone showed 100 and 97.5% of efficacies, respectively. However, the triflumizole and fernarimol showed relatively low curative efficacies of 30.8 and 51.6%, respectively. Microscopic observation of mycelial growth and conidia formation after detection of powdery mildew disease indicated lysis of mycelium and these lysis suppression of conidia formation showed tendency in accordance with curative efficacies of fungicides.

• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.1
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• pp.65-70
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• 2003

This study was conducted to select the promising varieties of alfalfa (va.) Venal, Ancho., P5444, DK120, DK125, Horizon, DK135, Luna and Kitawacaba at Daekwanryong branch(altitude 800m a.s.l.) and Namwon branch(altitude 450m a.5.1.) of National Livestock Research Institute Leaf width of alfalfa was broad in all varieties except fer Vernal, and DK125 was flowered the earliest of all varieties. Plant height of Luna was the longest, and winter hardness of P5444 was the greatest with 80.2% of all varieties. In Daekwanryong, dry matter(DM) yield of Luna was the highest and DM yield of vernal was the lowest among the varieties in Namwon. In both areas, dry matter yield ef P5444 was the highest of alfalfa varieties. In Daekwanryong, ADF and NDF concentrations of DK 120 were the lowest of all alfalfa varieties. In Namwon, ADF concentrations of Horizon was the lowest with 26％, and NDF concentrations of DK 125 and Horizon was the lowest with 40.8%. Crude protein concentrations of P5444, Luna and DK 120 were higher than that of different varieties in Daekwanryong and crude protein content of Horizon was the highest with 28.1% in Namwon. Average crude protein yield of both areas was the highest of P5444. The results of this study indicated that Luna, Horizon and P5444 would be the promising alfalfa varieties in Daekwanryong, Namwon and both areas, respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.1
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• pp.61-70
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• 2008

The object of this experiment was to investigate the food habits of sika deer fed with various roughage sources. The feeding trials were conducted at Chungnam National University and Unbong Animal Genetic Resources Station in 2007. The experimental roughages sources were five including 25 species in all; grass sources; 5 species(Lolium perenne, Festuca arundinacea, Poa pratensis, Agrostis alba and mixed grass), legume sources; 5 species(Trifolium pratense, Medicago sativa, Trifolium repens, Melilotus officinalis and Vicia villosa), browse sources; 5 species(Quercus serrata Thunb., Prunus jamasakura Sieb., Quercus aliena Blume, Robinia pseudoaccacia and Pinus densiflora), weed sources; 5 species(Artemisia princeps Pampanini, Erigeron canadensis, Alopecurus aegualis Sobolewski, Echinochloa crusgalli var. frumentacea(Roxb.) Wight and Rumex crispus), and native grass sources; 5 species(Zoysia japonica Steudel, Agropyron tsukushiense var. transiens(Hack) Ohwi., Arundinella hirta(Thunb.) Tanaka, Miscanthus sinensis Anderss and Phragmites comunis Trin). Five sika deer were selected which had nearly the same body weight(average 92.5kg) as experimental animal. Experimental roughages were prepared by 0.5kg(DM basis) per 1 species fed to sika deer, and the experiment was carried out from 08:00 to the next day 06:00. The chemical composition and dry matter digestibility of each roughage source and species were significantly different at the sampling area, plant species, growth stages and cutting period. The sika deer ate more roughages which had low fibrous contents, but high dry matter digestibility. Among all the 25 species of roughages, the favorite intake species ranking by sika deer was observed like this: Quercus aliena Blume, Prunus jamasakura Sieb, Quercus serrata Thunb. and Trifolium pratense, respectively, and the lower intake species was Phragmites comunis Trin. On the other hand, compared to each roughage source, total intake amount by sika deer was showed as browse(36.5%), Legumes(34.2%), grasses(14.0%), native grasses(13.5%) and weeds(1.8%), respectively. Based on the result, the food habits of sika deer seems to be closer to typically browser type.

• Horticultural Science & Technology
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• v.32 no.2
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• pp.217-226
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• 2014

Root-knot symptoms were found on a commercial tomato cultivar carrying Mi, a resistance gene to root-knot nematodes including Meloidogyne incognita, M. arenaria, and M. javanica in 2012 at Buyeo, Chungnam Province in Korea. The isolate was identified as M. incognita based on molecular analyses using two species-specific primer sets. Pathogenicity of the isolate on one susceptible and three resistant tomato cultivars to the root-knot nematodes was tested. The nematode isolate showed strong pathogenicity on all the tested cultivars at all tested incubation temperatures. In addition, resistance degree of 33 commercial tomato cultivars, 8 susceptible and 25 resistant cultivars to root-knot nematodes, was also tested. Plants were determined as resistant when they suppressed the nematode reproduction. All the cultivars demonstrated strong susceptibility to the nematode regardless of resistance of the tomato cultivars. To our knowledge, this is the first report on the occurrence of Mi infecting M. incognita isolate in Korea. On the other hand, to construct an efficient screening method for selecting resistant breeding source to the nematode isolate, root-knot development of M. incognita on four tomato cultivars according to several conditions such as inoculum concentration, plant growth stage, and incubation period after transplant was investigated. Reproduction of the nematode on all the tested cultivars according to inoculum concentration increased in a dose-dependent manner. Except for inoculum concentration, there was no significant difference in reproduction level of the cultivars according to the other tested conditions. On the basis of the results, we suggest an efficient screening method for new resistant tomato to the nematode isolate.

• Clinical and Experimental Pediatrics
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• v.48 no.7
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• pp.779-788
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• 2005

Purpose : Echinacea, a traditional plant medicine has been used as immune-stimulant. Recent studies have revealed that extract of Echinacea has immunostimulatory effects on human blood mononuclear cells. This study was designed for the purpose of screening the genes associated with immunologic effects of Echinacea on monocytes and dendritic cells using a cDNA microarray chip. Methods : $CD14^+$ monocyte cells were cultured for one day with Echinacea extract(final concentration : $50{\mu}g/mL$) in experiment 1, but were cultured without Echinacea in experiment 2. The gene expression of these cultured monocytes was analyzed using the cDNA microarray chip. Dendritic cells produced from $CD14^+$ monocyte were cultured for five days with GM-CSF and IL-4, and then cultured for one day with Echinacea in experiment 3, but were done without Echinacea in experiment 4. Results : In experiments 1 and 2, there were 17 significantly expressed genes with average expression ratios above 2.5, including interferon gamma-inducible protein 30(IFI 30), CDC(cell-division-cylcle)-like kinase 2(CLK 2), syndecan binding protein(syntenin), superoxide dismutase 2, etc. In experiments 3 and 4, there were 24 gene, with significantly expressed genes were 24 genes, which were insulin-like growth factor 2(somatomedin A), methyl-CpG binding domain protein 3, IFI 30, small inducible cytokine subfamily A, member 22, etc. The genes encoding CD44, IFI 30, mannose receptor C type 1(MRC 1), chemokine receptor 7(CCR 7), CLK 2, syntenin and cytochrome C oxidase subunit VIII were significantly expressed in both monocytes and dendritic cells cultured with Echinacea. Conclusion : This study employed a cDNA microarray chip to elicit the immune-associated gene profile; the expression was enhanced by Echinacea in CD14+ monocytes and dendritic cells. Thus we laid the basis for the quantitative and functional analysis of genes induced by Echinacea in monocytes and monocyte-derived dendritic cells.

• Microbiology and Biotechnology Letters
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• v.46 no.3
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• pp.253-260
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• 2018

Beneficial microorganisms are widely used in the forestry, livestock, and, in particular, agricultural sectors to control soilborne diseases and promote plant growth. However, the industrial utilization of these microorganisms is very limited, mainly due to uncertainty concerning their ability to colonize and persist in soil. In this study, the survival of beneficial microorganisms in field soil microcosms was investigated for 13 days using quantitative PCR with B. subtilis group-specific primers. Bacterial community dynamics of the treated soils were analyzed using 16S ribosomal RNA (rRNA) gene amplicon sequencing on the Illumina MiSeq platform. The average 16S rRNA gene copy number per g dry soil of Bacillus spp. was $4.37{\times}10^6$ after treatment, which was 1,000 times higher than that of the control. The gene copy number was generally maintained for a week and was reduced thereafter, but remained 100 times higher than that of the control. Bacterial community analysis indicated that Acidobacteria ($26.3{\pm}0.9%$), Proteobacteria ($24.2{\pm}0.5%$), Chloroflexi ($11.1{\pm}0.4%$), and Actinobacteria ($9.7{\pm}2.5%$) were abundant phyla in both treated and non-treated soils. In the treated soils, the relative abundance of Actinobacteria was lower, whereas those of Bacteroidetes and Firmicutes were higher compared to the control. Differences in total relative abundances of operational taxonomic units belonging to several genera were observed between the treated and non-treated soils, suggesting that inoculation of soil with the Bacillus strains influenced the relative abundances of certain groups of bacteria and, therefore, the dynamics of resident bacterial communities. These changes in resident soil bacterial communities in response to inoculation of soil with beneficial Bacillus spp. provide important information for the use of beneficial microorganisms in soil for sustainable agriculture.

• Journal of Bio-Environment Control
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• v.15 no.3
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• pp.231-238
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• 2006

The physical properties of seven perlites different in particle size distribution were investigated to develop perlite bag culture in Korea. Particle sizes of 1.0-2.8mm and larger than 2.8 mm were rather evenly distributed in S-1 (1.2-5 mm), S-2 (0.15-5 mm) and S-5 (parat No.1). Larger particles were less in S-3 (1-3 mm), S-4 (Parat No.2), S-6 (OTAVI) and S-7 (Agroperl B-3). S-4, S-6 and S-7 contained lots of particles less than 1 mm in size. Total porosity was similar among substrates with the value of $59{\sim}62%$. Container capacity was between 35-40% regardless of substrates except in S-2 with 27.7%. Water content, which was about 60% at 0 kPa, was decreased sharply at 4.90 kPa regardless of substrates, which meant the easily available water was plenty in any kind of perlite tested. Substrates, S-1, S-2 and S-3 with different particle size distribution, were investigated to evaluate for perlite bag culture. Six tomatoes (Licopersicon esculentum Mill. cv. Rokkusanmaru) were planted in a perlite bag of 40 liters with the dimension of 120cm in length and 34cm in width. The amount of nutrient solution supplied and its drainage dependent on daily integrated radiation didn't show any regular trend during the growth. Roots in the bag were distributed evenly in S-1 and S-2 than in S-3. Plant grown in S-1 showed the highest total and marketable yield of 8,628 and 7,759 kg/10a, respectively. The number of small size fruits and malformed fruits were more in S-3. Consequently, S-1 with the particle size distribution of 1.2-5 mm is suggested as desirable substrate for perlite bag culture.

• Journal of Bio-Environment Control
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• v.6 no.4
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• pp.250-257
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• 1997

Stem diameter and shoot fresh weight of tomato grown in greenhouse were measured non-destructively at 10 minutes interval from 1 to 16 July, 1996 with displacement detector using strain gauges and with suspension-type load cell, respectively, and simultaneously were measured soil water potential, transpiration and solar radiation. Ample water was irrigated before experiment, and thereafter, irrigations were made on the next morning when visual symptoms of wilting appeared. Shoot fresh weight and stem diameter showed very similar patterns in diurnal changes which are characterized by predawn maximum and afternoon minimum and in long- term evolutions, suggesting that stem diameter shrinkage and expansion are closely related to plant water content and growth, respectively, Shoot weight and stem diameter reached minimum values a little later than the time on which transpiration showed maximum. The daily net gains of fresh weight(DG) and stem diameter(DI) showed significantly Positive correlations with solar radiation in those days on which plants were not water-stressed. However, Dl and DG on those days of water stress showed much lower values than expected from the relationships between solar radiation and them. Transpiration was much lower than the expected potential transpiration on 10 July, implying that plants were water-stressed. In this case water stress was not detected from visual symptom of wilting and/or soil water potential, but was able to be identified by the lower DI and DG than the expected. The maximum contraction of stem diameter(MC) and the maximum loss of fresh weight(ML) during daytime showed significantly positive correlations with solar radiation in those days on which plants were not water-stressed and were observed greater than expected from the relationships on severely water-stressed days. But mild water stress could not be discernable by ML and MC. It would be concluded that the daily net gains of fresh weight and/or stem diameter could be used as criteria for diagnosing the water status of tomato and for triggoring the onset of irrigation in automatic system.

• The Korean Journal of Pesticide Science
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• v.6 no.4
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• pp.287-292
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• 2002

To establish effective and safe control method against Phytophthora root rot caused by Phytophthora capsici on tomato in hydroponic culture, three pesticides, oxadixyl copper hydroxide 8% WP, metalaxyl copper oxychloride 15% WP, and dimethomorph. dithianon 38% WP at 4 concentration levels were tested on potato dextrose agar medium inoculated with Phytophthora capsici. All pesticides inhibited mycelial growth, but two pesticides of them, metalaxyl copper oxychloride WP and dimethomorph. dithianon WP, were selected as effective pesticides for the efficacy test in a hydroponic culture. Forty days after transplanting of tomato seedlings, 4 ml of sporangia of P. capsici (about 25 sporangi/ml) per plot was inoculated around tomato plant root, and then 5 days after inoculation, the pesticides diluted at 5,000 times were drenched 1, 2 or 3 times per plot on the culture cube at 15 days interval. Fifteen days after drenching, tomato fruits and hydroponic culture solution were sampled for the analysis of pesticide residues. Dimethomorph was detected 0.001 and 0.003 mg/kg in tomato of the plots sprayed 2 and 3 times with dimethomorph dithianon WP of which detection levels were far below compared with 1.0 mg/kg of the Korean MRL of dimethomorph on tomato. Incidences of Phytophthora root rot were $30.5{\sim}50%$ in the plots drenched at 1 or 2 times with metalaxyl.copper oxychloride WP, and $16.7{\sim}25%$ in the plots treated with dimethomorph dithianon WP. However, there was no incidence of Phytophthora root rot in the plots treated at 3 times with both of pesticides, showing no phytotoxic effect. Based on the results, the drenching of these pesticides on the culture cube could be recommended as a very safe and effective control method for Phytophthora root rot in tomato.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.34 no.1
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• pp.118-128
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• 2016

The purpose of this study is to present efficient methods of preserving and managing the fish shelter forest in Mulgun-ri on the southern coast of Korea on the basis of its humanistic, sociological and ecological characteristics. The study object is Korean natural monument No. 150, which is presumed to have been forested by descendants of Jeonju Lee Family who settled there, and village rituals are held every October to pray for the peace of the village. The forest is managed by Namhae-gun as a historical and cultural resource as well as its disaster-preventing, economic, and environmental and ecological functions. The linear form of the area is $23,962.6m^2$ and farmland(48.5%) and urbanization area(38.2%) are extensively located in its periphery area. Actual vegetation was sub-classified into three types of land according to use pressure and whether or not damage was done: land where its stratification was formed; land where it was restored, and the land where it was damaged. Plant communities were sub-classified into Aphananthe aspera community(I) and Zelkova serrata community(II) which had a low use pressure; Z. serrata-Chionanthus retusa-A. aspera community(III) and A. aspera-Z. serrata community(IV) which had a high use pressure; and Celtis sinensis-A. aspera community(V) whose underlayer was damaged by use. Fragmentation of the forest is under way and its inside vegetation growth is hampered due to the installation of traffic and resting facilities such as the through roads costal roads, wooden-deck walkways, parking lots, washstands, etc. As a restoration management plan for this, the following were required: an establishment of preferred restoration area; a selection of restoration vegetation species; and an appropriate restoration method. The damaged area($7,868.2m^2$) will have to be set up as the preferred restoration area; seedlings of restored vegetation species should be raised with dominant species within the forest(i.e., Z. serrata, A. aspera, C. sinensis, and C. retusa) as their 'mother trees' for the benefit of for the next-generation forest; and sub-tree and shrub layer should be complementarily planted with 5 and 115 trees(unit $100m^2$) respectively to facilitate the formation of a multi-layered vegetation structure. In addition, resting facilities scattered inside the forest should be demolished; and indiscriminate use of them should be controlled; management and monitoring should be carried out so that the area can be preserved and restored as a deciduous broad-leaved forest.