• 한국약용작물학회지
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• 제25권3호
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• pp.139-145
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• 2017

Background: Invasion of these invasive plants in the ecosystem threatens the habitat of endemic species, reduces biodiversity, and causes a disturbance in the ecological system. Hypochaeris radicata L. (Asteraceae), the most invasive plant in Korea, particularly in Jeju Island, invades farmlands, and autochthonous forests, resulting in the establishment of monocultures and modification of the ecosystem structure. Methods and Results: In this study was, we evaluated the biological activity of 70% ethanolic extracts from different parts of Hypochaeris radicata L. The biological activities of 70% ethanolic extracts of different parts, such as flower, leaf, stem, and root, of H. radicata were investigated. The total polyphenol content was highest in flower extracts ($50.82{\pm}3.16mg{\cdot}QE/g$). In addition, the highest flavonoid content was observed in flower extract ($15.19{\pm}2.03mg{\cdot}QE/g$). The flower extract of H. radicata exhibited stronger DPPH radical-scavenging activities, ABTS radical scavenging activities, and reducing power than the other parts. The flower extract of H. radicata was observed to have the highest tyrosinase and ${\alpha}-glucosidase$ inhibitory activities. Conclusions: The flower extracts of H. radicata exhibited remarkable antioxidant activity as well as tyrosinase and ${\alpha}-glucosidase$ inhibitory effects. These activities might be related to the phenolic compounds present in the H. radicata flower extract.

• 대한본초학회지
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• 제28권5호
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• pp.69-77
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• 2013

Objectives : The purpose of this study was to characterize the effect of the Ethanolic extract of Stachys sieboldii and Lycopus lucidus on the learning and memory impairments induced by scopolamine. Methods : The genetic difference of Stachys sieboldii and Lycopus lucidus were observed with RAPD analysis. The cognition-enhancing effect of Stachys sieboldii and Lycopus lucidus was investigated using a passive avoidance test, Y-maze test and the Morris water maze test in mice. Drug-induced amnesia was induced by treating animals with scopolamine (1 mg/kg, i.p.). Results : As a result of RAPD analysis, Stachys sieboldii and Lycopus lucidus Radix was found to be genetically different and The results of learning memory analysis showed that Stachys sieboldii extract-treated group (500 mg/kg, p.o.) and the tacrine-treated group (10 mg/kg, p.o.) significantly ameliorated scopolamine-induced amnesia based on the Passive avoidance Y-maze test and Water maze test. And these results are same manner in DPPH radical scavenger effect and Acetylcholineseterase inhibition effect. These results suggest that Stachys sieboldii extract maybe a useful cognitive impairment treatment, and its beneficial effects are depending on the origin plants. Conclusions : Commercially available Stachys sieboldii Radix consists of two original plant, one of them people misuse. To clarify the origin of the plant Memory tests were performed. These results suggest that 80% Ethanol extract of Stachys sieboldii showed significant anti-amnestic and cognitive-enhancing activities related to the memory processes, and these activities were parallel to treatment duration and dependent of the learning models.

• 한국자원식물학회지
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• 제5권1호
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• pp.49-56
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• 1992

In the preliminary antitumor screening tests of Crude Drugs and collected plants, the methanol ic extract of Forsythia Fruits in Korean marcket showed signif icant cytotoxic activity against Chinese hamster V-79 cells, but that in Japanese market did not. The former was identifid as Forsythia viridissima Lindley and latter as F.suspensa Vahl on the basis of the morphological observation. When an aqueous solution of the extract prepared from the fruits of F. viridissima (Oleaceae) was partitioned successively with n-hexane, methylene chloride, n-butanol, the cytoyoxic activitywas concetrated in the methylene chloride extract. Fractionation of the extract was made with the guidance of bioassay against V-79 cells to give cytotoxic lignans, matariresiol (1) and arctigenin (2). Their ICso values of compounds 1 and 2 were respectively $7.8{\;}\mu\textrm{g}/ml$ 1 and $1.65{\;}\mu\textrm{g}/ml$. Also, their structures were confirmed by comparison of physical and spectral data in the literature.

• 한국자원식물학회지
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• 제35권6호
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• pp.713-719
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• 2022

Platycodon grandiflorum and Glycyrrhiza uralensis contain several bioactive compounds, such as saponin, oleanolic acid, and flavone. P. grandiflorum and G. uralensis have traditionally been used to treat disorders related to blood pressure, diabetes, and counteracting poison, and they have antinociceptive and antiinflammatory properties. However, the validity of complex saponin's vasodilatory effect has not been scientifically investigated. Therefore, this study explores the vasodilatory effect of complex saponin extracted from P. grandiflorum and G. uralensis mixture extract on rabbit carotid arteries. To this end, arterial rings with intact or damaged endothelium were used in an organ bath experiment and contracted by endothelin. Complex saponins, the major active constituents of P. grandiflorum and G. uralensis mixture extract, exhibited a moderate vasodilatory effect on the rabbit's basilar arteries. Therefore, treatment with complex saponin extracted from P. grandiflorum and G. uralensis mixture extract may selectively accelerate cerebral blood flow through basilar arterial dilation. Overall, the findings suggest that the extracted complex saponins can serve as vasodilator sources.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.119-119
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• 2022

The coronavirus disease 2019 (COVID-19) pandemic may be stressful for people. Public health actions, such as social distancing, can make people feel isolated and lonely and can increase stress and anxiety. As a result, there is a growing interest towards various materials to relieve stress. Thus, the present study aimed to investigate the anti-stress effects of ethanol extract of Ziziphus jujuba in PC12 cells treated with corticosterone and its underling mechanisms. Furthermore, the viability of the cells, the apoptosis of the cells, the level of phosphorylation of extracellular signal-regulated kinases (p-ERKs) expression were measured by MTT assay, LDH assay, Hoechst staining assay and western blotting. Our results showed that the extract of Ziziphus jujuba reversed corticosterone-induced damage in PC12 cells, which increased cell viability, decreased LDH release, and attenuated corticosterone-induced apoptosis as compared with the corticosterone-treated group. Therefore, these data suggest that the extract of Ziziphus jujuba could be a good candidate for development as a functional food supplement in the improve the anti-stress effect.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 추계국제학술대회
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• pp.73-73
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• 2020

Toona sinensis (TS) leaf is known to antinociceptive, antioxidative stress and skin moisturizing effects. Acnes vulgaris is a chronic skin disease with various symptoms including itchiness, pain and interruption of normal skin function. Propionibacterium acnes (P. acnes) is a major factor in the occurrence of inflammatory acnes. This study evaluated the antioxidant and anti-inflammation effects by TS extract from adventitious shoots. TS extract showed anti-inflammatory activities by suppression of pro-inflammation mediators (iNOS and COX-2) in LPS-stimulated RAW264.7 cells. TS extract also has anti-inflammatory activities by inhibiting the secretion of pro-inflammatory cytokines on P. acnes-stimulated HaCaT cells. These effects were regulated by MAPK signaling pathway. Therefore, we suggest that TS extract from adventitious shoots might have applications as a medicine for treating P. acnes-induced skin diseases.

• Journal of Microbiology
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• 제45권2호
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• pp.128-132
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• 2007

The red yeast Xanthophyllomyces dendrorhous (previously named Phaffia rhodozyma) produces astaxanthin pigment among many carotenoids. The mutant X. dendrorhous G276 was isolated by chemical mutagenesis. The mutant produced about 2.0 mg of carotenoid per g of yeast cell dry weight and 8.0 mg/L of carotenoid after 5 days batch culture with YM media; in comparison, the parent strain produced 0.66 mg/g of yeast cell dry weight and a carotenoid concentration of 4.5 mg/L. We characterized the utilization of carbon sources by the mutant strain and screened various edible plant extracts to enhance the carotenoid production. The addition of Perilla frutescens (final concentration, 5%) or Allium fistulosum extracts (final concentration, 1%) enhanced the pigment production to about 32 mg/L. In a batch fermentor, addition of Perilla frutescens extract reduced the cultivation time by two days compared to control (no extract), which usually required five-day incubation to fully produce astaxanthin. The results suggest that plant extracts such as Perilla frutescens can effectively enhance astaxanthin production.

• 한국식물병리학회지
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• 제1권1호
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• pp.56-60
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• 1985

본 실험은 질소 및 탄소원이 Cercospora kikuchii 균사생장에 미치는 영향과 여러 배양조건에서 생장한 C. kikuchii가 세균생장을 저지하는 정도를 알아보았다. C. kikuchii 균사생장에 적합한 질소원은 yeastextract였고, 탄소원으로는 sucrose, glucose, maltose, fructose를 첨가하였을 때 균사생장이 좋았다. C. kikuchii의 세균생장저지 실험에서 wild type은 pH 조건은 5.0에서, 질소원으로는 yeast-extract 또는 peptone.을 첨가하였을 때, 탄소원으로는 sucrose, maltose, glucose, fructose를 첨가한 배지에서 생장하였을 때 Erwinia carotovora와 Pseudomonas solanacearum의 생장을 크게 저지시켰다. 그러나 albino 돌연변이균주는 실허된 세균의 생장을 거의 저지시키지 못하였다.

• Plant Resources
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• 제8권3호
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• pp.188-193
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• 2005

In order to discern the possibility of functional food product or ingredient of a new medicine, the leaf parts and fruit parts of Prunus mume was partitioned with various solvents and their antioxidative activity was measured. When the antioxidative activity of MeOH extracts of leaf parts and fruit parts of Korea and China was compared, all of them showed the highest antioxidative activity in EtOAc fraction. In case of Korean Prunus mume leaf parts showed that quantity required for $RC_{50}$ to be $27.04{\mu}g$ in EtOAc fraction and in case of China Prunus mume leaf parts, it was $23.31\;{\mu}g$ which is similar to that of ${\alpha}$-tocopherol ($22.14\;{\mu}g$) and showed the highest activation. In case of Prunus mume fruit parts MeOH extract, Korean fruit showed $29.16\;{\mu}g$, and Chinese fruit showed $31.21\;{\mu}g$ in EtOAc fraction and thus Korean fruit extract showed a higher activity of antioxidant than the Chines fruit extract. When the antioxidative activity between the fruit parts and leaf parts of Prunus mume was compared, the leaf parts showed a higher antioxidative activity.

• 한국유기농업학회지
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• 제24권1호
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• pp.123-130
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• 2016

실내에서 복숭아씨살이좀벌 성충 침적법으로 유기농업자재에 의한 살충효과를 조사한 결과, 2014년에 'Extract of Sophora flavescens roots'가 84.7%의 살충률을 보였으며, 'Extracts of Neem II' 등 5개 자재는 100%의 살충률을 보였고, 2015년에 'Plant extract Matrine 0.5%+paraffin oil 8%'와 'Plant extract+pyrolignous acid Matrine 0.45%'가 각각 100, 94.6%의 보정살충율을 보였는데, 광양 다압 지역에서의 방제효과가 2014년과 2015년 4년 4월 21일에 살포시 방제효과가 좋아 실내와 포장에서 처리가 일치하는 경향을 보였고, Plant extract Matrine 0.5%+paraffin oil 8%'도 2015년에 실내와 포장에서의 살충효과가 유사한 양상을 보였다. 한편, 유기농업자재 살포시기에 따른 방제효과는 대체로 광양 다압 농가에서는 4월 하순 살포가, 순천 황전 농가에서는 4월 하순~5월 상순에 살포하는 것이 방제효과가 좋았는데, 광양 다압에서 2015년에 4월 21일과 27일 각각 1회씩 살포한 것과 2회 살포한 시험수에서 방제효과 차이는 없었으나 순전 황전 지역의 농가에서는 2회 살포한 시험수에서 방제 효과가 더 좋았다. 따라서, 유기재배 매실에서 살충효과가 있는 유기농업자재의 사용은 4월 중하순에 2회 이상 방제가 필요하고, 피해 양상이나 주변의 여건에 맞추어 방제전략을 수립하여야 할 것으로 생각된다.