• Journal of agriculture & life science
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• v.46 no.4
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• pp.69-84
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• 2012

To provide the basic information on the excavation, conservation, and systematic management plan for preservation of valuable forested wetlands, a field survey was analyzed at public and private forests in Daegu city, 2010. The expected points of FGIS were identified, and buffer zones for the protection of forested wetlands were derived. According to the results from the 11 points of forested wetland, the flora of wetlands in Daegu city were consisted of a total of 169 taxa; 63 families, 131 genera, 148 species, 2 subspecies, 14 varieties, and 5 forms. The species diversity of shrubs in forest wetlands was highest at 1.560, and the evenness was highest in shrub trees at 0.913. Considering the type of wetland, topography, etc., the buffer zone was set at 20~50m from the core area boundary.

• Journal of Plant Biotechnology
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• v.33 no.2
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• pp.139-145
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• 2006

The present study is the first report of molecular variations in different accessions of Rauvolfia tetraphylla L.f, a medicinally important plant collected from seven locations of Andhra Pradesh, India. Molecular analysis was carried out using RAPD markers. Out of the 40 primers screened from OPA and OPC Kts, a total of 205 scorable polymorphic markers out of 397 total markers were generated. Polymorphism of 51.6% was found with 3 unique markers. Levels of genetic diversity within accessions i.e., the genetic distance ranged from 0.816-0.932. Cluster analysis based on Dice coefficient showed two major groups indicating that mostly in cross-pollinated plants, high levels of differentiation among accessions exists independent of geographical distance. Hence the results of the present study can be seen as a starting point for future researches on the population and evolutionary genetics of this species. Understanding such variation would also facilitate their use in various conservational management practices, rootstock breeding and hybridisation programmes.

• Korean Journal of Plant Taxonomy
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• v.39 no.1
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• pp.48-54
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• 2009

We investigated the genetic diversity of an endemic rare species, Forsythia ovata Nakai by examining 93 ISSR amplicons in 84 individuals distributed among five populations. The overall percentage of polymorphic ISSR amplicons was 54.8% and mean number of amplicons per ISSR primer was 6.6. The amount of genetic diversity was relatively lower than other shrub species. The Mt. Seokbyeong and Mt. Seorak B populations had the highest level of genetic diversity. Although the Seokgae-jae population had the lowest level of genetic diversity, the population was genetically the most distinctive from the other populations. About 30.6% of the total variation was allocated between five populations, which was slightly higher than other shrub species. Such a pattern of genetic variation may have resulted from the limited distribution and small population sizes of F. ovata. The UPGMA dendrogram based on Nei's genetic distance showed some decisive geographic patterns. These results suggest that, in addition to the preservation of the natural stands, the conservation of larger number of populations with small number of individuals per population is more effective for the dynamic ex situ conservation and for maintaining the genetic diversity of F. ovata than smaller number of populations with large number of individuals.

• Journal of the Korean Institute of Landscape Architecture
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• v.31 no.5
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• pp.96-107
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• 2003

This study is to establish the Incheon Metropolitan City Arboretum Development Plan (IMADP) as a capacity building both for plant diversity conservation and nature friendly leisure activity areas of Incheon Metropolitan City, Korea. The arboretum planning area is located within territory of the Incheon Grand Park Based on the IMADP, the arboretum was divided into three display spaces; the Urban Greening Garden, the Rare and Endangered Plant Species Garden and the Theme Garden. Detailed planting guidelines were suggested according to the three garden areas. The Urban Greening Garden was designed to display the following three; the relevance techniques, the model, and the practical uses of plants that are regarded to an urban greening. The Rare and Endangered Plant Species Garden (REPSG) was planned for the collection and display of the rare and endangered native plants in the territories of Incheon and the island seashore. Also, the REPSG includes the education purposes of comparing and displaying taxonomically related plant species. The Theme Garden was projected for the collection and display of plant species that have long been familiar to Korean culture and which are also used in our daily life.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.63-63
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• 2019

Tea plant (Camellia sinensis (L.) O. Kuntze) has been cultivated widely in many developing Asian, African, and South American countries, where it is the most widely consumed beverage in the world next to water. It has critical importance to understand the genetic diversity and population sturcutre for effective collection, conservation, and utilization of tea germplasm. In this study, 410 tea accessions collected from South Korea were analyzed using 21 SSR markers. Among 410 tea accessions, 85.4% (310 accessions) accessions were collected from Jeollanam-do. A total of 286 alleles were observed, and the genetic diversity and evenness were estimated to be averagely 0.79 and 0.61, respectively, across all the tested samples. Using discriminant analysis of principal components, the four clusters were detected in 410 tea accessions. Among them, cluster 1 showed higher frequency of rare alleles (less than 1%) than other clusters. Using calculation of the index of association and rbaD value, each cluster showed a clonal mode of reproduction. The result of AMOVA showed that most of the variation observed was within populations (99%) rather than among populations (1%). Our results might contribute to provide data about genetic diversity for the conservation of tea germplasm and for future breeding programs.

• Horticulture, Environment, and Biotechnology : HEB
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• v.59 no.6
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• pp.875-888
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• 2018

Tulip (Tulipa L.) is one of the most important ornamental geophytes in the world. Analysis of molecular variability of tulips is of great importance in conservation and parental lines selection in breeding programs. Of the 70 genic microsatellites, 15 highly polymorphic and reproducible markers were used to assess the genetic diversity, structure, and relationships among 280 individuals of 36 wild and cultivated tulip accessions from two countries: Iran and the Netherlands. The mean values of gene diversity and polymorphism information content were 0.69 and 0.66, respectively, which indicated the high discriminatory power of markers. The calculated genetic diversity parameters were found to be the highest in wild T. systola Stapf (Derak region). Bayesian model-based STRU CTU RE analysis detected five gene pools for 36 germplasms which corresponded with morphological observations and traditional classifications. Based on analysis of molecular variance, to conserve wild genetic resources in some geographical locations, sampling should be performed from distant locations to achieve high diversity. The unweighted pair group method with arithmetic mean dendrogram and principal component analysis plot indicated that among wild tulips, T. systola and T. micheliana Hoog exhibited the closest relationships with cultivated tulips. Thus, it can be assumed that wild tulips from Iran and perhaps other Middle East countries played a role in the origin of T. gesneriana, which is likely a tulip species hybrid of unclear origin. In conclusion, due to the high genetic variability of wild tulips, they can be used in tulip breeding programs as a source of useful alleles related to resistance against stresses.

• Korean Journal of Plant Resources
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• v.20 no.6
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• pp.530-539
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• 2007

Genetic variation and structure of 9 subpopulations of Symplocarpus renifolius Schott ex Tzvelev on Mt. Cheonma, in Korea, were determined via starch-gel electrophoresis. The genetic diversity at 10 loci for 8 isozymes ($P_{99}=66%,\;A=2.26,\;H_o=0.212,\;H_e=0.230$) was found to be considerably higher than that seen in other long-lived perennial plants. On the whole, the genotype frequencies were in accordance with Hardy-Weinberg expectations. Approximately 5%($\theta=0.049$) of the total variability was among subpopulations. The high levels of observed genetic diversity in S. renifolius were attributed to a universal outcrossing system and other specific factors like differences in age classes and widely scattered individuals around the main distribution. Heterozygosity was highest at a mid-range of elevation($450m{\sim}600m$). The lowest heterozygosity at lower elevation was attributed to the possible origin of seeds transported by water from upstream regions during the monsoon season. Spatial structure in a subpopulation evidenced a strong autocorrelation between closer individuals within $3{\sim}4m$ of distance. This was assumed to be attributable to the restricted seed dispersal characteristics of S. renifolius. In accordance with the findings generated in this study, some implications regarding the conservation of S. renifolius at the Mt. Cheonma were also presented.

• Journal of Environmental Science International
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• v.33 no.6
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• pp.367-382
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• 2024

Alpine and subalpine plants are isolated and distributed in mountaintop areas at high altitudes and, are among the species most vulnerable to global warming. If the rate of warming continues at its current rate, it is highly likely that this species will be unable to find new refugia and will be the last to go extinct in their current habitats. Therefore, research on the distribution and diversity of alpine and subalpine plants is urgently needed and is important from the perspective of biodiversity conservation. Therefore, this study focused on the distribution of alpine and subalpine plants native to national parks. Alpine and subalpine plants distributed across the 12 national parks accounted for approximately half (47.78%) of all alpine plants in Korea. The average relative frequency of occurrence was 0.23, plant similarity between national parks was 37.19%, and beta diversity was 0.63. The number of species was positively correlated with the latitude, elevation, and area of the national park. Thus the geographical distributions of alpine and subalpine plants haves distinct spatial specificities and physical environmental differences.

• Journal of Plant Biology
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• v.38 no.2
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• pp.173-180
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• 1995

We investigated levels and distribution of genetic variation in Korean populations of Calystegia soldanella and C. japonica, clonally reproducing herbaceous perennials. Calystegia soldanella is one ofecologically important beach plants growing only on sand and beach dunes in Europe, East Asia, the Pacific Islands, and the west coast of North America. In contrast, C. japonica usually grows on small mounds of paddy fields, roadsides, and waste places with patchy distribution. Starch gel electrophoresis was conducted on leaves collected from 13 populations of C. soldanella and eight populations of C. japonica. The levels of genetic variation of the two species are very comparable; means of expected heterozygosity (Hep) were 0.100 and 0.099 for C. soldanella and C. japonica, respectively. These values were also very similar to those for species with similar life-history and ecological traits. However, the proportion of total genetic diversity partitioned among populations (GST) of C. soldanella (0.146) was considerably lower than that of C. japonica (0.383). In addition, means of Nei's genetic identity (Ⅰ) for C. soldanella and C. japonica were 0.985 and 0.900, respectively, which supports a restricted gene flow resulting from obligate clonal reproduction of C. japonica. Significant differences in allele frequency were detected among populations at eight and nine of nine polymorphic loci for C. soldanella and C. japonica (P<0.01), respecitvely. Considering the ecological importance of C. soldanella, the isolated beach populations coupled with present destruction of natural habitats of the species may result in erosion of genetic diversity in the near future. In this respect, conservation efforts should be focused on those populations that currently maintain the most genetic diversity such as those populations in the eastern and southeastern Korean Peninsula and Hamduck Beach, Cheju Island.

• Journal of Forest and Environmental Science
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• v.30 no.3
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• pp.253-258
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• 2014

The study attempts to assess the status, distribution, conservation and use value of Medicinal and Aromatic Plant (MAPs) in the Sagarmatha National Park. Altogether 62 species of MAPs belonging to 47 genera and 33 families have been recorded in the study area. 10 species, belonging to 9 families are categorized as the potential species. Out of the these species, most potential in local but threatened species are Allium hypsistum Stearn, Cordyceps sinensis Sacc, Dactylorhiza hatagirea Soo, Nardostachys grandiflora DC, Aconitum orochryseum, Ephedra gerardiana Wall. Ex. Stapf, Swertia multicaulis D. Don, Picrorhiza scrophulariflora Penne, Rheum australe. D. Don, Malva verticillataL and Swertia pedicallata Benerji. By analysis of data using Simpson's diversity index (SI) and Shannon weaver function (H'), there was high diversity (more heterogeneous) MAPs species composition in the Manjo Gate to Large Dobhan. (0.98349). Less diversity (less heterogeneous) MAPs species composition was in Tyanboche to Pangoche (0.90419). Similarly, the Shannon weaver function shows that in plots laying out in Mongla to Phorche are evenly distributed than others However, higher MAPs species (i.e., 31) was found in the way of Manjo Gate to Larja Dobhan than others.