• 제목/요약/키워드: Pit-1

검색결과 579건 처리시간 0.024초

Presence of Pituitary Specific Transcription Factor Pit-1 in the Rat Brain: Intracerebroventricular Administration of Antisense Pit-1 Oligodeoxynucleotide Decreases Brain Prolactin mRNA Level

  • Tae Woo Kim;Hyun-Ju Kim;Byung Ju Lee
    • Animal cells and systems
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    • 제3권3호
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    • pp.311-317
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    • 1999
  • Prolactin (PRL) was reported to be locally synthesized in many brain areas including the hypothalamus, thalamus (TH) and hippocampus (HIP). In the pituitary lactotrophs, PRL synthesis is dependent upon a pituitary-specific transcription factor, Pit-1. In the present study, we attempted to identify Pit-1 or Pit-1-like protein in brain areas known as the synthetic sites of PRL. Reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot analysis showed the same Pit-1 transcripts in brain areas such as the medial basal hypothalamus (MBH), preoptic area (POA), TH, and HIP with the Pit-1 transcripts in the anterior pituitary (AP). Electrophoretic mobility shift assay (EMSA) was run with nuclear protein extracts from brain tissues using a double strand oligomer probe containing a putative Pit-1 binding domain. Shifted bands were found in EMSA results with nuclear proteins from MBH, POA, TH and HIP. Specific binding of the Pit-1-like protein was further confirmed by competition with an unlabeled cold probe. Antisense Pit-1 oligodeoxynucleotide (Pit-1 ODN), which was designed to bind to the Pit-1 translation initiation site and block Pit-1 biosynthesis, was used to test Pit-1 dependent brain PRL transcription. Two nmol of Pit-1 ODN was introduced into the lateral ventricle of a 60-day old male rat brain. RNA blot hybridization and in situ hybridization indicated a decrease of PRL mRNA signals by the treatment of Pit-1 ODN. Taken together, the present study suggests that Pit-1 may play an important role in the transcriptional regulation of local PRL synthesis in the brain.

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생태적 축산폐수 처리 및 재활용 연못시스템의 Pit 메탄발효 (Methane Fermentation of Pit in Pond System for Ecological Treatment and Recycling of Animal Excreta)

  • 양홍모
    • 한국환경농학회지
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    • 제18권2호
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    • pp.191-195
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    • 1999
  • 유입폐수의 $BOD_5$가 1차연못에서 88%가 제거되고 있어 Pit의 $BOD_5$ 제거효율이 60%에 달할 것으로 예측된다. 메탄발효 Pit의 환경조건으로 용존산소가 없고, 혐기성 및 중성 pH가 유지되어야 하며, 충분한 체류시간이 확보되어야 하고, 온도변화가 적어야 한다. 분석결과 실험 메탄발효 Pit는 이런 조건들을 만족시키고 있어 Pit설계가 적절함을 알 수 있다. 실험결과 메탄발효 Pit의 설계인자로 폐수체류 기간이 2day, 월류유속은 $1.5m^3m^{-2}day^{-1}$가 적합하며, Pit바닥의 수심은 슬러지층의 온도와 밀접한 관계가 있어 3-3.5m 정도가 적합한 것으로 사료된다. Pit 바닥의 슬러지층 온도가 $16^{\circ}C$ 이상으로 유지되어야 메탈발효가 원활히 일어난다. 우리나라 중부지방과 기후조건이 유사한 지역에 위치한 연못시스템 연구에 의하면, 연못바닥의 온도가 메탄박테리아 활동이 거의 정지하는 $14^{\circ}C$ 이하로 내려가는 기간이 약 7개월이 된다. 온대권의 연못시스템은 연간 슬러지 침전량이 분해량보다 많아 어느 정도 슬러지가 쌓이게 된다. 따라서 여분의 30㎝ 수심을 두어 10-20년에 한번 슬러지를 제거하도록 설계한다. 실험 연못시스템이 설치된 장소는 중부지방보다 평균기온이 약 $3-4^{\circ}C$ 높은 지역으로 연못바닥 지하 1.5m에 위치한 Pit의 수온이 14℃이하가 되는 기간이 Fig.5에서 약 6개월이 된다. 실험 메탄발효 Pit는 좁은 면적의 연못에 설치하기 위해 콘크리트구조로 만들었으나, 1차연못의 규모가 크면 토공만으로 Pit설치가 가능하며 비용이 적게 든다. Pit에서 발생한 가스가 연못상층으로 이용하면서 $CO_2$가 해리되어 정제된 메탄을 회수할 수 있다. 메탄발생이 왕성한 기간에 연못상층에서 포집한 가스는 거의 메탄으로만 구성되어 있어 축산폐수를 처리하면서 메탄가스를 회수하여 연료로 사용하는 것이 가능하다. 메탄발효 Pit가 생태적으로 적응하면 초기보다 처리효율이 증가할 것으로 기대되어 지속적인 실험을 수행할 예정이다.

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Reliability and Validity of the Side-lying Instability and Prone Instability Tests in Patients with Lumbar Segmental Instability

  • Kim, Bo-Eon;Lee, Kwan-Woo;Park, Dae-Sung
    • 대한물리의학회지
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    • 제16권1호
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    • pp.1-7
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    • 2021
  • PURPOSE: The purpose of this study is to conduct inter-rater and intra-rater reliability tests in patients with low back pain (LBP) using the prone instability test (PIT) and side-lying instability test (SIT). We have analyzed the Korean version Oswestry disability index (K-ODI) correlations and radiograph finding (RF) for validity. METHODS: Individuals (n = 51) (mean age of 40.27 ± 13.28) with LBP for at least over a week were recruited, together with two participating physical therapist examiners. The measurement consisted of PIT, PST, K-ODI, and RF. Sensitivity (Sn), specificity (Sp), positive predictive value, negative predictive value, prevalence index, agreement %, Cohen's kappa, and prevalence-adjusted bias-adjusted kappa (PABAK) were calculated. The PIT and SIT were compared with RF for validity analysis, while PIT, SIT, K-ODI, and RF were calculated for the correlation analysis. RESULTS: The intra-rater reliability test measured for the PIT (kappa = .79, PABAK = .88) and SIT (kappa = .73, PABAK = .84), and inter-rater reliability test measured for the SIT (kappa = .80, PABAK = .88) showed good agreements. The PIT (Sn = .65, Sp = .63) and SIT validities (Sn = .68, Sp = .70) were compared with RF, showing a significant correlation in PIT and RF (r = .69), SIT and RF (r = .73), and PIT and K-ODI (r = .53). CONCLUSION: The SIT is a more comfortable position test than the PIT in patients. Both PIT and SIT have acceptable reliability and validity.

흰쥐 태반에서 Placental Lactogen I과 II 그리고 Pit-1의 유전자 발현에 미치는 에스트로겐의 영향 (Effect of Estrogen on the Gestational Profiles in Gene Expression of Placental Lactogen I, II and Pit-i in the Rat Placenta)

  • 정진권;강성구;강해묵;이병주
    • 한국동물학회지
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    • 제39권1호
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    • pp.115-121
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    • 1996
  • 임신중기에서 발기에 이르는 흰쥐의 태반에서 Placental Lactogen I (PL-I), II 그리고 Pit-1 의 유전자 발현 변화를 Northern blot hybridization으로 조사하였다. 그 결과, 임신시기에 따라 PL-I과 PL-II의 mRNA 양과 크기에 변화가 나타났다. 이들 유전자 발현에 미치는 에스트로겐의 영향을 조사하기 위하여, 임신 14일째 쥐의 난소를 제거하고(OVX), 이후 매일 에스트로겐을 투여한 후 (OVX+E), 임신 18일째 태반을 회수하여 PL-I, II 그리고 Pit-1의 유전자 발현을 Northern blot hybridization으로 조사하였다. OVX 군의 경우, PL-I의 mRNA 크기는 1 kb에서 1.3 kb로 PL-II의 mRNA는 0.6kb에서 1 kb로 변화하였다. OVX+E 군에서는 PL-I과 PL-II의 mRNA가 정상대조군과 같은 상태로 환원하였다. 정상대조군에 비하여 OVX와 OVX+E 군에서 PL-I과 PL-II의 mRNA 크기에는 영향을 미치지 못한 반면, mRNA 양은 난소제거시 감소하였다가, 에스트로겐을 투여하면, 부분적으로 회복되는 경향을 보였다. 이러한 본 실험의결과는 에스트로겐이 PL-I과 PL-II의 RNA splicing이나 polyadenylation 등을 포함한 유전자 발현에 영향을 미치며, Pit-1이 이 과정에 개입하는 것을 시사하는 것이다.

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Monitoring of Blood Cytokines by PIT-1 Genotypes in Day 150 Male Pigs

  • Yoon, J.;Rhee, M.S.;Seo, D.S.;Kim, B.C.;Ko, Y.
    • Asian-Australasian Journal of Animal Sciences
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    • 제14권12호
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    • pp.1659-1664
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    • 2001
  • Several studies have been done regarding carcass traits and growth in pigs. Recently, these have progressed to examine increases in economic traits, including meat quality and meat quantity, by using candidate genes. One of them is the pituitary-specific protein PIT-1, a member of the POU (Pit-Oct-Unc) family of transcription factors playing an important regulatory role in developmental processes. In addition, muscle development is known to be regulated in part by growth factors and cytokines locally produced. Therefore, studies were performed to analyze PIT-1 genotypes and serum cytokines (IGF-I, IGF-II, TGF-${\beta}1$, EGF, cortisol, DHEA-S, IL-2, and IL-6) in castrated male pigs for their possible involvement in the development of carcass traits. The genotypes of PIT-1 gene were analyzed by PCR-RFLP with MspI restriction enzyme. But, only CD and DD genotypes, not CC genotype, have been detected. Based on PIT-1 genotyping, a significant difference in EGF expression beween CD type (78.8 ng/ml) and DD type (46.0 ng/ml) was detected (p<0.05), whereas other cytokines did not show any statistical significance depending on PIT-1 genotypes. Collectively, these results suggest the possibility that EGF could affect the formation of carcass traits.

불특정 공식손상을 가진 316L 스테인리스강의 기계적 물성치 예측을 위한 다중선형회귀 적용 (Application of Multiple Linear Regression to Predict Mechanical Properties of 316L Stainless Steel with Unspecified Pit Corrosion)

  • 정광후;김성종
    • Corrosion Science and Technology
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    • 제22권1호
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    • pp.55-63
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    • 2023
  • The aim of this study was to propose a multiple linear regression (MLR) equation to predict ultimate tensile strength (UTS) of 316L stainless steel with unspecified pit corrosion. Tensile specimens with pit corrosion were prepared using a potentiostatic acceleration test method. Pit corrosion was characterized by measuring ten factors using a confocal laser microscope. Data were collected from 22 tensile tests. At 85% confidence level, total pit volume, maximum pit depth, mean ratio of surface area, and mean area were significant factors showing linear relationships with UTS. The MLR equation using these three significant factors at a 85% confidence level showed considerable prediction performance for UTS. Determination coefficient (R2) was 0.903 with training and test data sets. The yield strength ratio of 316L stainless steel was found to be around 0.85. All specimens with a pit corrosion presented a yield ratio of approximately 0.85 with R2 of 0.998. Therefore, pit corrosion did not affect the yield ratio.

전북지역 치과개원의의 열구전색제 사용실태에 관한 연구 (A STUDY OF ATTITUDES TOWARD AND USE OF PIT AND FISSURE SEALANTS BY DENTISTS IN CHON-BUK AREA)

  • 김재곤;백병주
    • 대한소아치과학회지
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    • 제21권2호
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    • pp.429-438
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    • 1994
  • Since the introduction of pit and fissure sealants in the early 1970's, many studies have been published amply documenting the efficacy of these materials as caries-preventive agents. However, the acceptance of the technique by dentist has been questionable. This survey was investigated the attitudes toward and use of pit and fissure sealants by dentist in Chonbuk area. The results were as follows: 1. Utilization of pit and fissure sealants is very high. Only 15% of dentists never use pit and fissure sealants, whereas 85% generally use pit and fissure sealants. 2. Most dentists reported that their knowledge of pit and fissure sealants was gained through dental schools education. 3. Concern about difficult to sell' to patient, poor retention of pit and fissure sealants and unsubstantiated by research were given as the main reasons why pit and fissure sealants was not used. 4. Patient demands and various educate program were given as important factors that would lead to increase use of pit and fissure sealants.

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복합조직강의 부식피트 성장특성에 미치는 식염수농도의 영향 (Influence of Salt Solution Concentration on Corrosion Pit Growth Characteristic of Dual Phase Steel)

  • 오세욱;강호민;김태만;도영민
    • 한국해양공학회지
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    • 제2권2호
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    • pp.78-86
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    • 1988
  • In order to investigate the corrosion pit occurrence and growth characteristic of M.E.F.(martensite encapsulated islands of ferrite) dual phase steel was made with a suitable heat treatment of raw material(SS41), a corrosion fatigue test was performed under rotary bending in the salt solution having a concentration from 0.01 wt percent to 3.5 wt percent. The fatigue strength of dual phase steel was remarkably decreased with an increase in concentration of salt solution; approximately from 63% to 80% in case of dual phase steel and from 40% to 71% in case of raw material. Corrosion pit occurred in the martensite phase and fatigue cracks from corrosion pits were selectively propagated in martensite phases. In the observation of corrosion pits at the origin of fatigue cracks, it had been found that corrosion pits were grown into hemispherical pits and a/c(the surface diameter, 2c and the depth, a of corrosion pit)was about 1.0-1.5regardless of the variation of salt solution concentration. The difference of corrosion pit depth growth rate was increased with an increase in concentration of salt solution according to an increase in stress level.

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콘크리트용 잔골재로서 산사.해사의 활용방안에 관한 연구 (A Study on the Use of Pit Sand and Sea Sand as the Fine Aggregate in Concrete)

  • 윤상대;신의근;배수호
    • 한국콘크리트학회:학술대회논문집
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    • 한국콘크리트학회 1993년도 봄 학술발표회 논문집
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    • pp.40-45
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    • 1993
  • Due to the recent shortage of river sand resulting from a rapid growth of concrete construction, sea sand and pit sand are increasingly used in stead. It is , however, well noted that non-washed sea sand used in reinforced concrete causes to corrode reinforcing steel and to incur cracks in concrete, and thus eventually result in damage to concrete. Moreover, many sources of pit sand in our country are randomly used without experimental research for its applicability to concrete construction. The purpose of this research to activate the usage of pit sand and sea sand for concrete construction to solve the recent shortage of river sand. Followings have been experimentally investigated : 1)Physical properties of pit sand and sea sand, 2)Compressive strength of mortar on the weight of pit sand passing through No.200 sieve, 3) Compressive strength of mortar on the chloride content of sea sand, 4) Compressive strengths of concrete using pit sand and sea sand, respectively, 5)Corrosion propagatio in reinforcing steel on the chloride concent, of sea sand, and 6)etc.

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국내에 서식하는 잉어과 어류의 Passive integrated transponder (PIT) tag 적용에 따른 생존율 평가 (Survival Rate of the Korean Cyprinidae Subject to Passive Integrated Transponder (PIT) Tagging)

  • 윤주덕;장민호
    • 생태와환경
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    • 제42권1호
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    • pp.134-138
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    • 2009
  • 국내에 서식하는 5종의 담수 잉어과 어류(참갈겨니, 피라미, 누치, 납자루, 끄리) 173개체를 대상으로 PIT 태그를 체내 삽입 후, 30일 간 매일 생존율을 파악하여 태그의 적용성과 활용 가능성을 타진하였다. 생존율 테스트 결과, 참갈겨니가 가장 높은 생존율을, 피라미가 가장 낮은 생존율을 보였으며, 전반적으로 개체의 전장이 길수록 생존 기간도 긴 것으로 나타났다. 초기 사망률은 외과적 수술과정에서 내부 장기의 손상 등과 관련이 있는 것으로 보였다. 본 실험결과 피라미를 제외한 국내에 서식하고 있는 소형 담수어류에도 PIT 태그 적용이 가능할 것으로 사료되며, 종에 따라 다소 차이는 있으나, PIT 태그의 야외현장 적용을 위해서는 전장 150mm의 개체를 사용하는 것이 적절할 것으로 판단된다.