• Journal of the Korean Society of Environmental Restoration Technology
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• v.9 no.2
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• pp.72-80
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• 2006

This study was conducted to evaluate the influence of dried swine excrement on the germination of Pinus thunbergii seeds, the growth response of seedlings of Pinus thunbergii and the uptaken of nitrogen and phosphorous by seedlings of Pinus thunbergii. The germination rate of seeds of Pinus thunbergii tends to decrease according to the increasing of application amount of dried swine excrement and the application amounts of dried swine excrement which is more than 3%(w/w %) makes the rate of germination to much more decreased. Contents of nitrogen and phosphorus are much higher in a way that the dried swine excrement was treated in nursery soil. 179 days after seeds of Pinus thunbergii were sowed, nitrogen contents in soil was decreased more than 70% and phosphorus was decreased 20 to 45%. Growth response of Pinus thunbergii was much higher in treatment of dried swine excrement than in control. But growth response was not affected by increasing of application amounts of dried swine excrement. Growth response of Pinus thunbergii was the highest in 2%(w/w %) application but its growth response was decreased in treatment more than 3% (w/w %) of dried swine excrement.

• Research in Plant Disease
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• v.17 no.2
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• pp.177-183
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• 2011

Four-year-old seedlings of Pinus thunbergii, Pinus densiflora and Pinus rigida were inoculated with Fusarium circinatum isolate (FT-7), the pitch canker fungus, from P. thunbergii, to evaluate the effects of the pathogen on disease development and gas exchange rate. Needle dehydration was evident on 2 of 10 seedlings of P. thunbergii and P. rigida at 18 and 21 days after inoculation, respectively, while no symptoms were observed in P. densiflora seedlings throughout the experiment. Gas exchange stopped completely in 4 of 5 measured seedlings of P. thunbergii and 2 of 5 measured seedlings of P. rigida at 25 days after inoculation, and in the remaining 3 seedlings of P. rigida at 39 days after inoculation. Disease development in P. thunbergii seedlings was faster than that in P. rigida seedlings. By the time, the experiment was ended at 78 days after inoculation, 9 of 10 seedlings of P. rigida and 8 of 10 seedlings of P. thunbergii seedlings treated with FT-7 was almost dead, but all seedlings of P. densiflora were still healthy. We suggest that P. densiflora is resistant to F. circinatum in the current study, and gas exchange rate of the species after inoculation does not differ significantly compared to that of untreated control.

• Journal of Korean Society of Forest Science
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• v.98 no.5
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• pp.558-562
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• 2009

The growth, carbon and nitrogen status of container grown black pine (Pinus thunbergii) seedlings were examined at various levels of foliar fertilization (control, 0.1%, 0.2%, 0.3%). Root collar diameter, height and dry weight of black pine seedlings increased significantly with increasing levels of foliar fertilization (P<0.05). Carbon concentration in needle of black pine seedlings was significantly higher in the foliar fertilization than in the control treatments (P<0.05), while other seedling components such as stem and roots were not significantly different (P>0.05) between the foliar fertilization and the control treatments. Nitrogen concentration and content were significantly greater in the foliar fertilization than in the control treatments (P<0.05). Shoot/root ratio of black pine seedlings (needle+stem dry weight/root dry weight) was greater in the foliar fertilization (2.40-2.89) than in the control treatments (1.87). However, nitrogen use efficiency was significantly lower (P<0.05) in the foliar fertilization (28-46) than in the control (111) treatments. The results indicate that morphological characteristics and nutritional status on container grown black pine seedlings were enhanced by various levels of foliar fertilization.

• The Plant Pathology Journal
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• v.29 no.1
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• pp.116-122
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• 2013

To evaluate the mechanisms of pathogenicity and nonpathogenicity of Bursaphelenchus xylophilus and B. mucronatus isolated in South Korea, we used 4-year-old P. thunbergii seedlings and 20-cm long one-year-old stem cuttings of 5-year-old Pinus thunbergii, and studied distributions and multiplications of pine wood nematodes after inoculation. The distributions of B. xylophilus in the 20-cm pine stem cuttings were not significantly different from that of B. mucronatus. Conversely, the proliferation rate of B. xylophilus on mycelial mats of Botrytis cinerea was significantly different from that of B. mucronatus. The study using 4-year-old P. thunbergii seedlings also showed that B. mucronatus can migrate to distal portions of the pine seedlings the same as B. xylophilus, but the populations of B. xylophilus remaining in the pine seedlings were relatively larger than those of B. mucronatus. Therefore, we concluded that the pathogenicity of B. xylophilus could be strongly influenced by its ability to multiply.

• Journal of Forest and Environmental Science
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• v.34 no.2
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• pp.196-198
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• 2018

This study aimed to evaluate the effectiveness of jellyfish chips in promoting the survival and growth of Pinus thunbergii seedlings in a coastal area based on a five-year field investigation from 2012 to 2016. Seedling survival rate was significantly higher in the treatment (96%) than in the control (75%) group in 2012. Furthermore, the height of five-year-old seedlings after plantation in 2012 was significantly greater in the treatment (3.41 m) than in the control (2.32 m) group (t=10.151; p-value<0.01). These results revealed that jellyfish chips can improve soil condition by enhancing moisture retention during the early growth stages and supplying nutrients to the seedlings over time. Our findings indicated that jellyfish chips could be used effectively as an organic fertilizer for growing coastal disaster prevention forests.

• The Plant Pathology Journal
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• v.26 no.4
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• pp.299-305
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• 2010

A half-sib family of two 4-year-old seedlings of Pinus $\times$ rigitaeda was inoculated with each of 20 Pinus thunbergii isolates of Fusarium circinatum (syn. Fusarium subglutinans f. sp. pini) from two pitch canker damaged sites in Jeju Island, South Korea. Initial symptoms of needle damages were visible on most of the seedlings at 18 days after inoculation. The 20 tested isolates were not significantly different in virulence, based on lesion lengths at the site of inoculation (P = 0.217). The most virulent isolate FT-7 showed the longest lesion length. Some seedlings began to die 46 days after inoculation. All seedlings were dead by 68 days after inoculation except two seedlings inoculated with each of isolates FS-2 and FS-13, respectively. Using the FT-7, 38-year-old 11 P. $\times$ rigitaeda trees, which were survived from a seed orchard severely damaged by pitch canker, were inoculated on branches in the seed orchard in Jeju Island to assess differences in susceptibility to pitch canker. The 11 trees differed significantly (P < 0.001) in susceptibility to F. circinatum based on average lesion lengths measured 56 days after inoculation. It is possible that induced resistance contributed to their capacity to limit lesion development. The susceptibility of natural selection P. $\times$ rigitaeda trees are more likely affected by interaction with F. circinatum rather than environmental conditions.

• Journal of Korean Society of Forest Science
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• v.69 no.1
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• pp.51-55
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• 1985

Pinus thunbergii ${\times}$ P. massoniana $F_1$ hybrids were made by pollinating 15 years old Japanese black pine (P. thunbergii) with Masson pine (P. massoniana) pollen, air-shipped from Taiwan. The crossability between two species were observed. Seedling height, morphological characteristics, and the composition of phenolic compounds of these hybrids and the open-pollinated progenies of P. thunbergii were investigated. The results of the observation were as follows; 1) Number of the hybrid seeds per cone was 2.3, while open-pollinated Japanese black pine produced 26 seeds per cone. 2) All hybrid seedlings outgrew P. thunbergii, averaging 151% of mean height of Japanese black pine seedlings. The growth of hybrids was much variable, ranging from 120% to 208% of mean height of the open-pollinated progenies of P. thunbergii. 3) With regard to needle characteristics, the hybrid needles were longer and softer than those of P. thunbergii. The color of hybrid needles showed yellowish green. The positions of resin ducts in P. thunbergii observed medial, while those of hybrids showed medial and external. 4) No large difference in phenolic compound composition between hybrid and P. thunbergii was found, but phenolic compound of unknown 4 and 6 were observed only in hybrid. Further intensive work is recommended in this area.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.420-428
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• 2009

In this study, the variation of 20 Pinus thunbergii isolates of F. circinatum from 2 damaged sites in Jeju-Island were compared with a known Fusarium circinatum using molecular biological techniques. Two- and four-year-old seedlings of Pinus rigida${\times}$Pinus x rigitaeda and two-, three- and six-year-old seedlings of P. thunbergii were inoculated with one of the most virulent isolates, FT-7, to determine differences in susceptibility. In site 1 (FT), 13 isolates of F. circinatum were isolated from 14 individuals and in site 2 (FS), 7 isolates of F. circinatum were isolated from 9 individuals. No difference was found in the sequences of the internal transcribed spacer (ITS) region of the ribosomal RNA genes in the FS and FT isolates, and also even in the known isolate of F. circinatum, FE 1-1. However, the ITS sequences of the FS and FT isolates differed from those of a fungus, Botrytis cinerea. Two-year-old seedlings of P. rigida${\times}$P. x rigitaeda showed higher susceptibility (93.3% of mortality) than four-year-old ones. Three-year-old seedlings of P. thunbergii showed the highest susceptibility (66.7% of mortality) compared to those at other ages in the same species. We found a positive correlation between basal diameter and lesion length in the seedlings of P. rigida${\times}$P. x rigitaeda ($R^2=0.66$) and P. thunbergii (p < 0.0001), respectively. There were significant differences in susceptibility by the age of seedlings in each of P. rigida${\times}$P. x rigitaeda (p < 0.0001) and P. thunbergii (p < 0.0001) based on lesion length.

• Journal of Korean Society of Forest Science
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• v.99 no.2
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• pp.172-178
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• 2010

The status of ectomycorrhizal (ECM) fungal colonization in Pinus thunbergii seedlings was investigated 2 years after planting in an eastern coastal area of Korea. We established three $10{\times}10$ m plots at a P. thunbergii plantation in Gangneung and sampled lateral roots from 10 seedlings in each plot. ECMs were classified into morphological groups and the number of root tips of each morphotype was counted. In total, 8 ECM morphotypes were observed and fungal species that form each morphotype were identified by sequencing of the internal transcribed spacer (ITS) region of the nuclear rDNA. Suillus granulatus was the most abundant species (44.1-65.7% of relative abundance) in all plots, followed by Tomentella ellisii (14.0-37.8%) and unidentified fungus belonged to Atheliaceae (10.6-20.1%). These 3 fungal species accounted for almost all of the ECM abundance in each plot (94.9-99.8%). The remaining 5 fungal species were uncommon and rare. There was no clear difference in ECM fungal communities among plots. Community structure of ECM fungi in the young P. thunbergii plantation was simple and composed of fungal species that were also observed in mature coastal pine forests.

• Journal of Practical Agriculture & Fisheries Research
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• v.12 no.1
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• pp.3-10
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• 2010

In order to promote the growth of the trees, it is the most important to fertilize efficiently. The organic nutrient "Green-one" which list public notification number 08-organic-3-112" was registered in Rural Development Administration. The "Green-one" is the environment friendly organic nutrient with new concept which is used in planting and maintenance of the trees. The "Green-one" makes the tree grow actively. Also it has a function of the anti germ and sustainable fertilizer effect. In conclusion, "Green-one" promoted the growth of P. thunbergii excellently. Expecially the growth of the root increased. The concentration of "Green-one" dilution was more better 100 times than 300 times in trees grow. This research can apply with the test of fertilizer efficiency in landscape trees.