• Korean Journal of Plant Resources
• /
• v.17 no.2
• /
• pp.189-201
• /
• 2004

The present study has measured eight pollen morphological parameters of Diploxylon species in Korea by light microscopy (LM). The results are as follows; 1. Diploxylon species in Korea showed significant (P＜0.01) interspecific difference in their pollen morphological parameters. 2. The discriminant analysis based on the pollen morphological parameters demonstrated that the classification ratio of Diploxylon was 49.9％. The maximum was at Pinus banksiana (72.8％) and the minimum was at P. sylvestris (62.2％). 3. The relationship among the Diploxylon species based on their pollen morphological parameters showed that P. densiflora and P. sylvestris were had the closest relationship while P. rigida and banksiana had the least relationship.

• Journal of Plant Biology
• /
• v.29 no.3
• /
• pp.185-196
• /
• 1986

Anatomical characteristics of the secondary xylem in the roots and the stems of genus Pinus including nine species growing naturally in Korea have been studied to elucidate the structural differences between two organs. The results, comparing the root woods with the stem woods, are as follows; The transition from the early-wood to the late-wood occurs more gradually in the root woods. In the root woods, the bordered pits on the radial wall of tracheid are frequent in the two rows. The spiral thickening on the radial and tangential wall of the tracheid can be seen in the only stem woods of four species such as P. koraiensis, P. rigida, P. rigitaeda and P. banksiana. In the majority of the species studied, the length of the tracheid is longer in the root woods, but in some species such as P. strobus, P. densiflora, P. rigida and P. sylvestris, this length is almost sam ein both organs. The diameter of the tracheid in the root woods is wider. The wall thickness of the tracheid in the early wood is thicker in the root woods, but vice versa in the late woods. More rays per unit area can be seen in the root woods.

• Preventive Nutrition and Food Science
• /
• v.15 no.1
• /
• pp.36-43
• /
• 2010

The purpose of this study was to investigate the phenolic acid, proanthocyanidin (PAs), and flavonol glycoside contents, as well as the antioxidant activities of pine needle extracts from six species of young pine trees. The extracts were prepared from Section Pinus (Diploxylon): P. densiflora, P. sylvestris, P. pinaster and P. pinea, and Section Strobus (Haploxylon): P. koraiensis and P. strobus. Phenolics were extracted from pine needles with 80% acetone to obtain the soluble free fraction, and insoluble residues were digested with 4 M NaOH to obtain bound ethyl acetate and bound water fractions. Phenolics were analyzed by HPLC, and the hydrophilic antioxidant activity was measured using oxygen radical absorbance capacity (ORAC). Total phenolic and flavonoid contents of the soluble free fraction were higher than those of the bound ethyl acetate and bound water fractions. The main phenolics were monomers and polymers of PAs in the soluble free fraction, and phenolic acids and flavonol glycosides in bound ethyl acetate fraction. Flavonol glycosides found in different species of pine needles were qualitatively similar within fractions, but composition varied among Pinus sections. High levels of kaempferol arabinoside and an unknown compound were present in all Strobus species. The soluble free fraction had the highest antioxidant activity, followed by bound ethyl acetate and bound water fractions.

• Journal of the Korea Furniture Society
• /
• v.26 no.3
• /
• pp.222-228
• /
• 2015

To evaluate the natural decay durability of valuable domestic softwoods which are used for preservative treatment in our country, we carried out decay test by European standard method. Of all test wood species, Japanese larch (Larix leptolepis) showed slightly high natural decay durability compared to other 4 wood species, Japanese red pine (Pinus densiflora), pitch pine (Pinus rigida), Japanese cedar (Cryptomeria japonica), and scots pine (Pinus sylvestris). However, all of evaluated domestic softwood species in this study caused high weight losses over about 30% in heartwood by test fungus, Poria placenta. We can hardly expect a good natural decay durability from these softwood species. According to the classification of the natural durability of European standard (EN 350-1), they are classified into "Not durable" or "Slightly durable". Therefore, if using these softwoods as exterior materials, we must do preservative treatment to ensure durability.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.30 no.1
• /
• pp.75-86
• /
• 2002

This study was conducted to analyze the change of soil characteristics effect on the condition of urban forest in Sasang parti located near Sasang Industrial Complex. The results of this study are as follows; 1. Soil hardness is increasing from the area of forest, to the entrance, to facilities in that order. Soil acidity pH4.19∼4.23 in Sasang park indicated a high acidity condition. High levers of K, Na, Mg, Ca are shown in the areas composed of high soil hardness. 2. Pinus thunbergii in the overstory tree layer, Alnus japonica in the understory tree layer, and Rhus sylvestris in the shurb layer are shown respectively as dominant species based on the ground survey and the compution of important value. Pinus thunbergii is decreasing, while Alnus japonica and increasing. 3. Oplismenus undulatifolius which has a strong tolerance for air pollution, is shown as a dominant species of herbaceous plants in Sasang park. There are 10 species of Harbaceous in Sasang park compared to 20 species in Molundae park. This shows that deversity in herbaceous plants are imported by air pollution. 4. Species diversity indices of Sasang park is 0.8738∼0.9700 compared to 1.0817∼ 1.233 in Molundae park is due to the good condition of soil environment in addition to air pollution effects. 5. The vitality of Pinus thunbergii is 16.41∼20.42ER in Sasang park, and 12.42∼ 16.81ER, in Molundae park. This shows that tree vitality are impacted by soil characteristics. The regression analysis between tree vitality and soil environment shows the effects of is soil hardness, soil moisture, soil acidity, K, Na, Mg, Ca.

• Proceedings of the PSK Conference
• /
• 2003.10b
• /
• pp.194.2-194.2
• /
• 2003

The inhibitors of prostaglandin biosynthesis and nitric oxide production by corresponding inducible isozyme have been considered as potential anti-inflammatory and cancer chemopreventive agents. In our continuous search for cancer chemopreventive agents from natural products, we have evaluated the inhibitory potential of PGE$_2$ and NO production in lipopolysaccharide (LPS)-induced mouse macrophage RAW 264.7 cells. As a result, pinosylvin (3,5-dihydroxy-trans-stilbene), a stilbenoid, mainly found from the heartwood and leaves of the Pinus sylvestris, showed potential inhibitory activity of LPS-induced PGE$_2$ and NO production in a dose-dependent manner. (omitted)

• Journal of Korean Society of Forest Science
• /
• v.62 no.1
• /
• pp.82-86
• /
• 1983

Cone and seed development after self-pollination in 14-year-old Pinus rigida Mill. was investigated in an $F_1$-hybrid seed orchard that consists of Pinus rigida Mill. and Pinus taeda L. in alternative rows. On an average, 83.8% of the self-pollinated receptive female strobili developed into cones. This value appears to be normal compared to cone development after open-pollination in other pine species such as in Pinus sylvestris L. However, there was a great variation in filled seed development after self-pollination among the investigated individual trees (Table 1-1). The 34.5%, a ratio of filled seeds after self-pollination was significantly lower than 91.7%, that by the open-pollination. Some of the investigated trees developed 70.0%-83.5% of filled seeds after self-pollination. Thus, those are not desirable as trees for seed parent in establishing $F_1$-hybrid seed orchards in future. A further study on the rate of filled seed development by various pollination systems consisting of self-pollination, self-and cross-pollination with intraspecific, mixed (tree's own and foreign pollens) pollens and cross-pollination with interspecific, mixed pollens of the two parental tree species is necessary to determine the quality of seed parent trees in establishing $F_1$-hybrid seed orchards.

• Journal of Korean Society of Forest Science
• /
• v.104 no.4
• /
• pp.549-557
• /
• 2015

Novel cpSSR primers were developed based on the sequence information of the Pinus koraiensis chloroplast genome. A total of 30 cpSSR loci were detected in the chloroplast genome, and a total of 30 primer sets flanking those loci were designed. All primer sets were successfully amplified for chloroplast DNA in P. koraiensis. The cross-species transferability of the 30 primer sets was considerably high in P. pumila (100%) and P. paviflora (97%) belonging to the same Subgenus (Strobus) of P. koraiensis. Meanwhile, the transferability was relatively low (73%) in P. densiflora and P. sylvestris belonging to Subgenus Pinus. A total of 13 cpSSR loci out of the 30 loci were polymorphic in the Mt. Jumbong population of P. koraiensis. The mean of haploid diversity(H) was 0.512. The number of haplotypes(N) and the haplotype diversity($H_e$) were 25 and 0.992, respectively. Of the 25 haplotypes, 22 were unique in the analyzed population. The unique haplotypes differentiated 22 individuals (79%) from the total of 28 individuals. In conclusion, the novel cpSSR primers developed in this study would be applicable to other Pinus species, especially the subgenus Strobus, and provide a high level of polymorphism for the study of genetic variation of P. koraiensis.

• Journal of agriculture & life science
• /
• v.45 no.6
• /
• pp.213-226
• /
• 2011

This study was performed to investigate the effect of essential oils and ethanolic extracts of approximately 650 plant species on acetylcholine esterase (AChE) enzyme activity using Ellman's colorimetric method in 96-well microplates. The results showed that the ethanolic extracts from twig of Sophora subprostrata, twig of Phellodendron amurense, seed of Corylopsis coreana, and essential oil (EO) from Citrus paradisi, Cupressus sempervirens, Ocimum basilicum, Pinus sylvestris and Rosmarinus officinalis inhibited more than 80% of AChE activity. Among these, EO from Pinus sylvestris, C. sempervirens and C paradisi exhibited higher values of AChE inhibitory activity, which were 75, 84 and 99% at a concentration of 50 ug/ml, respectively. Finally, EO from C paradisi (grapefruit, GEO) showed the highest inhibitory activity towards AChE, which showed 91% of inhibition at a concentration of 20 ug/ml. We also examined the anti-dementia effects of GEO in mouse by passive avoidance test and Morris water maze test. The model mouse (male, ICR) of dementia (negative control) was induced by administration of scopolamine (1 mg/kg body weight). The latency time of sample group administrated with GEO (100 mg/kg, p.o.) increased significantly as compared with negative control on passive avoidance test. There were significant recovery from the scopolamine-induced deficits on learning and memory in water maze test through daily administrations with GEO (100 mg/kg, p.o.). From these results, we conclude that GEO treatment might enhance the cognitive function, suggesting that the EO of C. paradis may be a potential candidate for improvement of perceptive ability and dementia.

• The Korean Journal of Ecology
• /
• v.19 no.4
• /
• pp.285-294
• /
• 1996

Ring-width chronologies of Scots pine (Pinus sylvestris L.) and Norway spruce (Picea abies (L.) Karst.) from two experimental forest plots in Kaunas, Lithuania were developed to study tree growth-climate relationship in different geohydrological conditions using response function analysis. The tree ring-width chronologies of Scots pine ranged from 1883~1987 A.D. and 1864~1989 A.D., and Norway spruce 1838~1987 A.D. and 2870~1989 A.D., respectively. The response function analysis has vividly demonstrated that the growth of Scots pine is favoured by warm summer and Norway spruce by cool and moist summer. Spring temperature has shown direct relationship with tree growth of both the species. There also exists notable intraspecies analogies in growth responses except some minor differences.