• 제목/요약/키워드: Pinus sp.

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곰솔(Pinus thunbergii)의 침엽에서 분리한 내생균의 분자적 동정 (Molecular Identification of Endophytic Fungi Isolated from Needle Leaves of Pinus thunbergii)

  • 김창균;어주경;엄안흠
    • 한국균학회지
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    • 제40권4호
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    • pp.183-186
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    • 2012
  • 곰솔(Pinus thunbergii Parl.)의 잎에 서식하는 내생균의 다양성을 확인하기 위하여 충남 보령의 해안가에 서식하는 곰솔에서 잎을 채취하여 총 19개의 균주를 분리하였다. 분리된 내생균을 ITS 지역과 LSU 지역의 염기서열을 분석하여 동정한 결과 Anthostomella sepelibilis, Beltrania sp., Cladosporium sp., Coniochaeta velutina, Entonaema pallida, Lophodermium sp., Nigrospora sphaerica 등 총 7개 분류군의 내생균을 확보할 수 있었다. 이들 중 Beltrania sp.에 속하는 종이 가장 높은 빈도로 분리되었으며 내생균의 서식 환경이 곰솔의 잎에 서식하는 내생균의 분포에 영향을 미칠 수 있음을 확인하였다.

한국의 잣나무에서 분리한 내생균의 다양성 (Diversity of Fungal Endophytes from Pinus koraiensis Leaves in Korea)

  • 서상태;김경희;김명주;홍진성;박종한;신상철
    • 한국균학회지
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    • 제37권1호
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    • pp.108-110
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    • 2009
  • 영월과 진천의 2지역에서 잣나무로부터 내생균의 다양성을 조사하였다. 총 113개의 내생균을 분리하여 rDNA ITS 부위의 염기서열 분석으로 균을 동정하였다. 염기서열 분석결과 Pestalotiopsis sp.가 2지역에서 가장 높은 빈도로 분리되었다. 영월에서는 Pestalotiopsis sp. 68.5%, Lambertella sp. 12.4%로 분리되었으며, 진천에서는 Pestalotiopsis sp. 41.7%, Hypoxylon sp. 20.8%, 그리고 Phomopsis sp.가 12.4% 분리되었다. Hypoxylon sp.와 Phomopsis sp.는 영월에서 분리되지 않았다.

Molecular Identification of Endophytic Fungi Isolated from Needle Leaves of Conifers in Bohyeon Mountain, Korea

  • Yoo, Jae-Joon;Eom, Ahn-Heum
    • Mycobiology
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    • 제40권4호
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    • pp.231-235
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    • 2012
  • Fungal endophytes are microfungi that live in plants without causing apparent symptoms of infection. This study was conducted to identify endophytic fungi isolated from leaves of coniferous trees in Bohyeon Mountain of Korea. We collected leaves of two species of coniferous trees, Pinus densiflora and Pinus koraiensis, from 11 sites in the study area. A total 58 isolates were obtained and identified using molecular and morphological characteristics. Four species of endophytic fungi were isolated from P. densiflora: Lophodermium conigenum, Leotiomycetes sp., Septoria pini-thunbergii, and Polyporales sp., while two fungal species were isolated from P. koraiensis: Eurotiomycetes sp. and Rhytismataceae sp. The most frequently isolated species were L. conigenum and S. pini-thunbergii.

강원 중부지역 산림내의 딱정벌레과(科) 분포에 관한 연구 (Distribution of Carabid Beetles(Coleoptera: Carabidae) in different forests of Central Kangwon-do)

  • 장석준;김종국
    • Journal of Forest and Environmental Science
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    • 제16권1호
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    • pp.42-49
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    • 2000
  • 임상이 서로 다른 임분에서 딱정벌레군집 구조를 해석하였다. 딱정벌레과의 전체 종수 및 개체수는 소나무림에서 10속 23종 1,1627개체, 잣나무림에서 11속 23종 418개체, 일본잎갈나무림에서 11속 25종 782개체, 참나무림에서 12속 25종 1,383개체 이었다. 소나무림에서 우점종들은 Synuchus cycloderus(19.8%), Synuchus nitidus(16.6%), Pterostichus pertirax(16.2%), 잣나무림 Pterostichus sp. 1(27.9%), Synuchus nitidus(24.4%), Synuchus cycloderus(10.2%), 일본잎갈나무림 Synuchus sp.1(40.0%), Synuchus nitidus(9.3%), Pterostichus sp.1(7.4%), 참나무림 Pterostichus sp.2(17.4%), Synuchus cycloderus(14.4%), Pterostichus pertirax(14.1%) 이었다. Shannon지수 (H') 및 균등도지수(J')는 소나무림 2.11, 0.64 잣나무림 2.25, 0.72, 일본잎갈나무림 2.23, 0.69, 참나무림 2.32, 0.72로 조사되었으며, 유사도지수는 잣나무와 참나무림에서 0.66으로 낮았으며, 소나무림과 일본잎갈나무림에서 0.83으로 가장 높았다.

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보은 지역의 소나무(Pinus densiflora)에서 분리한 내생균의 동정과 다양성 (Molecular Identification and Diveristy of Endophytic Fungi Isolated from Pinus densiflora in Boeun, Korea)

  • 길이종;어주경;엄안흠
    • 한국균학회지
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    • 제37권2호
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    • pp.130-133
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    • 2009
  • The endophytic fungi were isolated from red pine trees (Pinus densiflora) that were distributed in three sites of Chungbuk, Korea. Twenty fungal isolates were isolated from 16 trees and divided into 8 groups by morphological characters. The fungal isolates were identified using the sequences of ITS region of rDNA; Lophodermium complex, Sydowia polyspora, Hymenula sp., Sistotrema brinkmannii, Septoria pini-thunbergii, Earliella sp. Lophodermium spp. were the most frequently found fungal species the across sites and firstly detected from Pinus species in Korea by molecular work.

개량(改良)펄프화법(化法)으로 제조(製造)된 폐액(廢液)중의 리그닌의 특성(特性) (Lignin Characterization of Waste Liquor by Modified Pulping Process)

  • 황병호;조헌정;이종윤
    • Journal of the Korean Wood Science and Technology
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    • 제23권1호
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    • pp.13-20
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    • 1995
  • This study was carried out to investigate the characterization of lignins from waste liquors in SP, KP, ASAM, and AS from Pinus densiflora, Quercus mongolica, and Betula ermanii. Spectroscopic study was applied to examine the lignins separated from different pulping process. Lignin contents in waste liqours increased in order of AS, ASAM, KP, and SP. UV spectra of three types of lignin except AS lignin showed similar pattern. IR spectra of AS lignin showed strong C=O absorptions in the range from 1730 to 1750$cm^{-1}$, where as those of KP, SP, and ASAM showed weak stretch in this region. NMR spectra of AS lignin showed strong characteristic chemical shifts of acetoxyl groups of acetylated aliphatic and aromatic hydroxyl groups at 2.0~2.5 ppm. Molecular weight of ASAM lignin from Pinus densiflora determined and found number average molecular weight 1,199, weight average molecular weight 5,458. z average molecular weight 17,242, and viscosity average molecular weight 5,457. It is considered from the results based on spectroscopic study of lignin that waste liquors (in SP, KP, ASAM and AS) from Pinus densiflora, Quercus mongolica, and Betula ermanii can be used for lignin utilization.

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대구 동천동 청동기시대 취락유적 출토 목질류 수종 분석 연구 (Study of the Species of Trees Used for Wooden Artifacts Excavated from a Bronze-Age Settlement Site in Dongcheon-dong, Daegu)

  • 이효선
    • 박물관보존과학
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    • 제20권
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    • pp.49-60
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    • 2018
  • 대구 칠곡지구 일대 유적은 청동기시대 및 삼국시대 대규모 취락지가 확인된 고고학적 조사로 그 의의가 크다. 특히 대구 동천동 취락유적에서는 청동기시대 수혈주거지, 고상건물지, 석관묘, 우물, 집수기, 집석, 구상유구, 경작지, 환호, 하도 등의 유구가 확인되어 청동기시대 당시 취락의 전모를 알 수 있는 많은 자료가 조사되었다. 본 조사는 대구 동천동 취락유적 내 청동기시대 하도 유구 출토 목기 8점에 대한 수종분석 결과이다. 조사 결과, 침엽수재 수종인 소나무류(Pinus spp.)가 2점, 전나무속(Abies spp.) 수종이 2점, 활엽수재 수종인 상수리나무류(Quercus sp.)가 4점 식별되었다. 용도미상으로 분류된 3점의 목기는 소나무류와 전나무속 수종이였으며, 건축부재 5점은 소나무류가 1점, 상수리나무류가 4점이었다.

First Report of Pitch Canker Disease on Pinus rigida in Korea

  • Lee, Jong-Kyu;Lee, Sang-Hyun;Sung-II Yang;Lee, Yin-Won
    • The Plant Pathology Journal
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    • 제16권1호
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    • pp.52-54
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    • 2000
  • Pitch canker of Pinus rigida, caused by Fusarium subglutinans f. sp. pini, was first noticed in Inchon, Korea, and is now being spread into other plantations of pines. Typical symptoms re resin flows from canders on the shoots, branches, and trunks, and resin-soaking of the sap wood under the bark. Pathogenic fungi were isolated from the infected shoots, branches, and sees on Fusarium-selective medium. Mycelial growth and microscopic characteristics were examined. Pathogenicity test was carried out by inoculating four common species of pines (P. rigida, P. densiflora, P. thunbergii, and P. koraiensis) in Korea. P. rigida and P. thunbergii showed symptoms identical to those of naturally infected trees, while P. densiflora and P. koraiensis remained free symptomless.

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Analysis of Cone and Seed Characteristics from Different Mating Design Strategies of Pinus densiflora for. multicaulis

  • Lee, Kyungmi;Lee, Hyunseok;Kang, Jun-Won
    • Journal of Forest and Environmental Science
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    • 제37권4호
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    • pp.309-314
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    • 2021
  • This study aimed to enhance seed productivity and secure genetic resources for Pinus densiflora for. multicaulis. We analyzed the characteristics of cone and seed generated by control pollination between Pinus densiflora (PD) and Pinus densiflora for. multicaulis (PDM). The highest number of cone scales (63.0) was obtained from the self-pollinated (sp) PDM clone B (PDM-sp-B), whereas the lowest number of cone scales (44.7) was obtained from two combinations designated as PDM-A×PD-075 and PDM-A×PD-0111. Both female parents of the hybrids were PDM-A. The highest seed production capacity (80.8) was obtained from the open-pollinated (op) PDM clone B (PDM-op-B). The seed potentials of PDM-B×PD-0111, PDM-op-A, and PDM-sp-B were 67.4, 66.5, and 63.1, respectively. The highest number of fertile scales (41.5) was obtained from PDM-op-B, and the lowest number of fertile scales (28.8) was obtained from PDM-A×PD-075. The total number of aborted ovules and 1st aborted ovules was not statistically significant in the mating design. The cross combination of PDM-B×PD-0111 had the highest number (34.8) of filled seeds and the lowest number of 2nd aborted ovules (5.2) and empty seeds (9). PDM-op-B had the highest number of developed seeds (47.6), although the number of empty seeds was the highest (41.2). Therefore, we conclude that the mating design of PDM-B×PD-0111 is useful for future breeding programs to improve seed yield of PDM. Our results showed that there was a strong correlation between the following two parameter pairs: number of scales and number of fertile scales, and the number of fertility scales and seeds potential (r=0.89 and r=0.84, respectively; both p<0.01).

종로 청진 12~16지구 출토 나막신의 수종분석 (Species Identification for Excavated Wooden Shoes in the 12~16 Chungjin District of Jongno)

  • 오정애;박정민;김병로
    • 한국가구학회지
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    • 제26권2호
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    • pp.163-170
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    • 2015
  • This study was conducted to identify wood species for 51 excavated wooden shoes in the 12~16 Chungjin district of Jongno. Wood species of 31 Pinus sp., 12 Torreya nucifera S. et Z., 4 Ginkgo biloba L., 2 Salix sp., 1 Kalopanax pictus (Thunb.) Nakai. and 1 Morus sp. were identified. Wood used for wooden shoes was a species that can be mainly obtained easily around. Among them, Torreya nucifera S. et Z. grows in warm temperate zone of Korea, which shows that interregional cooperation has occurred.