• Title/Summary/Keyword: Pinus roots

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Growth-Inhibiting Effects of Various Traditional Drinks of Plant Origin on Human Intestinal Bacteria

  • Jeon, Ho-Joung;Roh, Jung-Yeon;Kim, Do-Hyoung;Lee, Wan-Kyu;Ahn, Young-Joon
    • Applied Biological Chemistry
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    • v.41 no.8
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    • pp.605-607
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    • 1998
  • A total of 28 traditional drinks derived from 23 plant species in 19 families were tested for their in vitro growth-inhibiting effects against Bifidobacterium adolescents, Bifidobacterium bifidum, Lactobacillus acidophilus, Lactobacillus casei, Clostridium perfringens, and Escherichia coil using a paper disc agar diffusion method under anaerobic condition. The responses varied with bacterial strain, plant species and tissue sampled. In a test with C. perfringens at 5 and 10 mg/disc, potent growth inhibition was produced from the extracts of Eucommia ulmoides stems, Pinus densiflora leaves and shoots, Thea sinensis leaves (green and oolong teas) and Zingiber officinale roots. All materials tested did not adversely affect the growth of bifidobacteria, lactobacilli, and E. coli. These results may be an indication of at least one of the pharmacological activities of these plant-derived drinks.

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ITS Primers with Enhanced Specificity to Detect the Ectomycorrhizal Fungi in the Roots of Wood Plants

  • Kim, Dong-Hun;Chung, Hung-Chae;Ohga, Shoji;Lee, Sang-Sun
    • Mycobiology
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    • v.31 no.1
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    • pp.23-31
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    • 2003
  • With universal primer ITS1-F, the specific DHJ2 primer was developed to detect the Ectomycorrhizal(ECM) root tips in soil and to identify the species of ECM fungi, as based on DNA sequences of rDNA stored in GeneBank of NCBI. This primer was designed with the common sites of rDNA of Amanita and Boletus, and was also designed with several DNA programs provided by NCBI. The DNA fragments synthesized by PCR were calculated to be 1,000 to 1,200 bps of DNA located to 18s to 28s rDNA to contain two variable sites of ITS, indicating much diversities for specific species or ecotypes of ECM fungi. The primer DHJ2 reacted with the genomic DNA's extracted from the tissues of basidiocarp at the rate of 73 of 80 fungi collected produced single bands with a 1,100 bps length. The DNA fragment synthesized with the genomic DNA that extracted from eight ECM tips of Pinus densiflora was confirmed and analysized to the rDNAs of ECM in full sequences, and informed to be a ECM fungal species in the forest.

Accumulation of Heavy Metals(Cd, Cu, Zn, and Pb) in Five Tree Species in Relation to Contamination of Soil near Two Closed Zinc-Mining Sites (아연폐광산(亞鉛廢鑛山) 주변(周邊) 토양(土壤)의 중금속(重金屬) (Cd, Cu, Zn, Pb) 오염(汚染)에 따른 5개(個) 수종(樹種)의 부위별(部位別) 중금속(重金屬) 축적(蓄積))

  • Han, Sim Hee;Hyun, Jung Oh;Lee, Kyung Joon;Cho, Duck Hyun
    • Journal of Korean Society of Forest Science
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    • v.87 no.3
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    • pp.466-474
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    • 1998
  • This study was conducted to evaluate heavy metal concentrations(Cd, Cu, Z, and Pb) in the soil of two zinc mines and to correlate heavy metal contents between the soils and trees growing near the mines. Soils and leaves, stems, and roots of five tree species(Corylus heterophylla, Pinus rigida. Populus alba${\times}$glandulosa, Rhododendron mucronulatum, and Robinia pseudoacacia) were collected from Sambo Zinc Mine located in Hwasung and Gahak Zinc Mine in Kwangmyung city in Kyonggido. Soils near two zinc mines were not seriously contaminated by heavy metals, but Zn and Pb concentrations were at toxic level. The heavy metal concentration in soils decreased in the order of Zn, Pb, Cu, and Cd, and decreased with increasing distance from zinc mining sites. Among the five tree species, Populus alba ${\times}$ glandulosa showed the highest heavy metal concentrations in the tissue except for Pb. Particulars, leaves of the species contained the high concentrations of heavy metals to reach the maximum of 91ppm Zn. The roots of Corylus heterophylla contained high concentrations of Cu and Pb. The order of heavy metal concentrations in the tree species was Zn, Cu, Pb and Cd. The concentration of heavy metals in the tree tissues showed a positive correlation with that in soil in which trees are growing. The ratio of heavy metal concentration of trees to that of soils(concentration factor : CF) was highest in Zn and lowest in Pb. Populus alba${\times}$glandulosa had the highest CF value among the five tree species. It was concluded that Populus alba${\times}$glandulosa, based on the high metal uptake ability, could be used for decontaminating of heavy metals from contaminated soils, and Pinus rigida could be used to reflect the level of contamination in soils.

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Plant Species Utilization and Care Patterns Using Potted Plants in the Traditional Gardening (전통조경에서 분(盆)을 이용한 식물의 활용과 애호 행태)

  • Kim, Myung-Hee
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.31 no.3
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    • pp.61-74
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    • 2013
  • This study examined and analyzed ancient writing and poetry regarding cases of appreciating plants by using pots in a garden of a palace or private houses by ancestors, and examined shape and planting method of plant species and potted plants, arrangement and preference of potted plants. As for the method of the study, description research method which examines and interprets poem and painting based on potted plants. The results of this study is summarized like the following. First, the plants which were favorably used for potted plants include 19 kinds such as Prunus mume, Pinus densiflora, Pinus pumila, Phyllostachys spp., Camellia japonica, Punica granatum, and Gardenia jasminoidesa, and as for herbs, 12 kinds such as Chrysanthemum monifolium and Nelumbo nusifera . Second, the species which were specially arranged into artificial shapes include Prunus mume and Pinus densiflora. The two plants made the shape of severe curves of stems such as Wangpi. Gyuban, and Bangan, and there are Pinus densiflora dwarfed potted plant whose roots are stretched on Prunus mume grafted into a strange stump and an oddly shaped stone. For the beauty of the dwarfed tree shape, pine cones are added to an old Pinus densiflora or Parthenocissus tricuspidata is planted to stems, and additional method of making moss on the soil, which is called 'Jongbunchuigyeong'. As for planting method, water culture, planting on a stone, planting on a charcoal, and assembled planting are expressed in poetry. Third, as for external space for potted plants, a place where a king stays, a bed room for a king, surrounding areas and gardens of private houses, and step stones were used as a space which adds artistic effects. Potted plants are placed on a table in a library, on a desk, on a drawer, and near a pillow as a small items in a room, and scholars enjoyed original characteristics and symbolism of the potted plants. Fourth, at the time of flowering of Prunus mume, poetry event was held to enjoy the tree and writing poetry begun. And at the time of flowering of Chrysanthemum monifolium, the flowers were floated in a liquor glass or shadow play was enjoyed. Fifth, potted plants played the role of garden ornaments in elegant events of a palace, the gentry, wedding ceremony, and sacrificial rites. Sixth, potted plants were used as tributes between countries, donation to a king, or a gift of a king. In addition, there were many cases where scholars exchanged potted plants and there is the first record of giving a potted plant in 'Mokeunsigo' by Mokeun Isaek, scholar in the late era of Goryeo. Seventh, at the time of flowering Prunus mume, Chrysanthemum monifolium, Gardenia jasminoides, Nelumbo nusifera, and Narcissustazetta var. chinensis, they enjoyed the particular fragrance and express it into poetry. Eighth, plant species from southern parts such as Camellia japonica, Daphne odora, Gardenia jasminoides, Citrus unshiu, Phyllostachys spp., Punica granatum, Rosa rugosa, and Musa basjoo, or foreign plant species, and species weak against the cold were utilized as pot plants for enjoying green trees indoors in northern central province in harshly cold winter.

Biomass Expansion Factors, Allometric Equations and Stand Biomass of Pinus thunbergii in Southern Korea (전남 여수지역 곰솔의 현존량 확장계수, 상대생장식 및 임분 현존량)

  • Park, In-Hyeop;Kim, So-Dam
    • Korean Journal of Environment and Ecology
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    • v.32 no.5
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    • pp.507-512
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    • 2018
  • Three natural Pinus thunbergii stands in southern Korea were studied to investigate stem density, biomass expansion factors, allometric equations and stand biomass. Stand ages of stand 1, 2 and 3 were 15, 29 and 45 years old, respectively. Three $10m{\times}10m$ plots were set up, five sample trees were cut and roots of three sample trees were excavated for dimension analysis in each stand. Stem density of stand 1, 2 and 3 were $0.450g/cm^3$, $0.440/cm^3$ and $0.457g/cm^3$, respectively, and there was no significant difference among the three stands. Biomass expansion factors of above-ground and total tree decreased with increasing stand age. Above-ground biomass expansion factor of stand 1 was significantly higher than those of stand 2 and 3, and total tree biomass expansion factor of stand 1 was significantly higher than that of stand 3. Allometric equations were developed for the 15 sample trees of the three stands based on D or $D^2H$. Above-ground biomass of stand 1, 2 and 3 were 50.72t/ha, 89.92t/ha, 194.07t/ha, respectively, and total tree biomass of stand 1, 2 and 3 were 61.62t/ha, 113.12t/ha, 248.36t/ha, respetively.

Studies on Charateristics of Pinus densiflora Forest in Kangwon Province (II). Constructive and Maintenance Respiration as Related to Growth of Saplings (강원도(江原道) 소나무림(林)의 특성(特性)에 관한 종합적(綜合的) 연구(硏究)(II) 유령목(幼齡木)의 생장(生長)에 따른 구성호흡(構成呼吸)과 유지호흡(維持呼吸))

  • Han, Sang Sup;Chang, Chun Geun;Kim, Sun Hee
    • Journal of Korean Society of Forest Science
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    • v.83 no.2
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    • pp.221-228
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    • 1994
  • Respitation and growth rates of leaves, branches, stems and roots of 2 to 11-yr-old red pine trees(Pinus densiflora) were examined and applied to Thornley's growth equation, $$R=(\frac{1-Yg}{Yg})\frac{dW}{dt}+mW$$. The conversion efficiency of substrates(Yg), maintenance respiration coefficients(m), relative growth rates(${\mu}$) were estimated. The efficiency of conversion of substrates (Yg) was 0.3637g/g dw/yr and the maintenance respiration coefficient(m0 was 0.094g/g dw/yr. The relative growth rate(${\mu}$) was remarkably reduced with age from 0.90(2-year-old) to 0.33:11 year-old). The Ratio of gross respiration(R) per gross photosynthesis(Pg), R/Pg showed the range of 0.6~0.7 and annually 64% of Pg was spent for constructive respiration. The 3.4% of dry weight of whole tree was spent for maintenance respiration.

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Production and Transplanting of Ectomycorrhizal Pine Seedlings Using the Old Fairy Ring of Tricholoma matsutake (기존 송이 균환(菌環)을 이용한 송이균 감염 소나무의 생산 및 이식)

  • Ka, Kang-Hyeon;Hur, Tae-Chul;Park, Hyun;Kim, Hee-Su;Bak, Won-Chull;Yoon, Kap-Hee
    • Journal of Korean Society of Forest Science
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    • v.95 no.6
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    • pp.636-642
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    • 2006
  • To make a new fairy ring of Tricholoma matsutake in situ, the way of production and transplanting of ectomycorrhizal seedlings of T. matsutake using Pinus densiflora was investigated after transplanting from 2000 to 2005 as well as the method to improve their survival rate for the fungus. For the production of ectomycorrhizal pine seedlings, the seedlings planted at the edge of fairy ring of T. matsutake in November showed 97% of the survival rate, while those planted in April showed 80% of the rate. For the complete infection of the T. matsutake, it required more than two years after planting. The infection rate of mycelia for the ectomycorrhizal seedlings was 17.6% when the natural seedlings were used, whereas it was relatively low when the seedlings prepared from the nursery were used. The survival of T. matsutake mycelium reached up to 22% by the transplanting in April, while the mycelium transplanted in October and November showed less than 5% of the survival. The survival of T. matsutake on the transplanted seedlings was the highest in the seedlings having 50% of infection rate before transplanting. Excavation of the ectomycorrhizal seedling to examine the vitality of ectomycorrhizal roots of T. matsutake resulted in the perishing of them. Therefore, the method using a 'rice bag triers' to check living mycelium of T. matsutake without digging of transplanted seedlings was introduced in this study. In addition, it is recommended that the examination has to be conducted at least two years after transplanting.

On the rooting of the cuttings of two conifer species (송백류삽수(松柏類揷穗)의 발근(發根)에 관(關)한 연구(硏究))

  • Yim, Kyong Bin
    • Journal of Korean Society of Forest Science
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    • v.1 no.1
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    • pp.12-21
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    • 1962
  • Outdoor cuttings studies on two coniferous species, pitch pine and needle fir, have been made at Suwon, Korea extending from March to early May, 1956. The purpose of these studies was to find the effects of ages of parent trees, topophysis, dilute hormone solution treatments and the time at which the cuttings were collected on the rooting response of the cuttings. On September 2, rooting results were examined. Pitch Pine (Pinus rigida) The total of 1335 cuttings were collected from 3- and 10-year old trees. The cuttings treated were planted on March 13 and April 20. 1. In the cuttings collected from 3-year-old seedlings, the best result (34 percent rooted) was obtained by treating ends of cuttings with a mixture of 50 ppm IBA and 50 ppm NAA. 2. No rooting was observed in lammas shoots. 3. The cuttings from 3-year-old seedlings gave better rooting than those from 10-year-old trees. 4. The root origins were developed from the lower part of the cutting and the cut surface. Needle fir (Abies holophylla) One hundred twenty-five cuttings were collected from a 30-year-old tree. The cuttings treated with any one of four mixed hormone solutions were planted on March 23. 1. Fifteen cuttings out of 75 cuttings from the lower half of the crown were rooted. The cuttings obtained from the upper half of the crown did not root at all. 2. The cuttings treated by the hormone solution made up of 20 ppm IAA, 20 ppm IBA, 20 ppm NAA, 20 ppm thiamine and 5 percent sucrose gave the best rooting (22 percent). 3. All roots originated from callus tissues and irregularly arranged parenchymatous tissues near the cut base.

Morphological Characteristics Analysis of Root Plate in Wind-Uprooted Trees (풍도목 근분의 구조형태학적 특성 분석)

  • Kim, Dongyeob;Ahn, Byungkyu;Kim, Myeong Pil;Im, Sangjun
    • Journal of Korean Society of Forest Science
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    • v.103 no.2
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    • pp.248-257
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    • 2014
  • The objectives of this study were to identify the root plate dimension of wind-uprooted trees and to analyze the relationship among wind direction, aboveground and belowground properties of the trees. The root plates of 77 Japanese larches (Larix kaempferi) and 24 Korean pines (Pinus koraiensis), which were uprooted by a typhoon in 2012, in the Taehwa Experimental Forest of Seoul National University, Korea, were investigated. The results showed the root plate shape could be assumed to be an oval or a circle in above view, and half an ellipse in side view, respectively. Also, the number and surface area of individual roots in root plates were greater in uprooting direction than in non-uprooting direction. The results of correlation analyses between aboveground and belowground properties indicated DBH had more significant correlation with belowground properties than tree height. Finally, simple linear relationships were derived for significantly correlated tree aboveground and belowground properties.

The 18s rDNA Sequences of the Basidiocarps of Tricholoma matsutake in Korea (한국산 송이버섯에서의 18s ribosomal DNA 서열)

  • Lee, Sang-Sun;Hong, Sung-Woon
    • The Korean Journal of Mycology
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    • v.26 no.2 s.85
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    • pp.256-264
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    • 1998
  • The 18S rDNA sequences of Tricholoma matsutake (TM=T. caligatum var. nauseoum) collected in Korea were analyzed for the ectomycorrhizal fungi in the roots of Pinus densiflora. The 514 base pairs of rDNA region were synthesized by UF-5 and UR-6 primers, and double checked in the base pair. The sequence of four strains synthesized were all identical in this work, but different from those done by the previous workers. The basidiocarps collected in this work. were identified to T. matstake after searching the 18s rDNA by the BLAST in NCBI. Only several base pairs of 18S rDNA analyzed from other related basidiocarps were different from our analyses of 18S rDNA. The dendrogram were made based on the sequences of the 514 bp 18S rDNA by CLUSTAL-X alignment program. The groupings of the species at the level of genus in the dendrogram were well constructed.

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