• Journal of Korean Society of Forest Science
• /
• v.99 no.3
• /
• pp.359-367
• /
• 2010

The objective of this study was to find optimal planting season of the effect of vegetation competition around planting tree. This study evaluates the possibility of extending the planting period of containerized seedling of Pinus densiflora (1-0 year). We planted seedling at three different seasons (spring, summer, fall) at 18 plots in 3 locations. Planting sites were composed of vigorous vegetation (Site A) and less vigorous vegetation (Site B). For over 3 years, the study investigated survival rate, growth of root collar diameter and height, and biomass of containerized seedling of P. densiflora. In all sites, containerized seedling of P. densiflora showed high survival rate in summer planting. Height and root collar diameter of containerized seedling of P. densiflora were the highest in summer planting, but in the initial period after planting growth was not good. After time passed, growth rate was increased. Site B showed better growth than Site A. Biomass was the highest in summer planting and also the highest in Site B. These results suggest that planting period can be extended by using containerized seedling and vegetation control in the initial is very important for survival and growth of containerized seedling.

• Journal of Korean Society of Forest Science
• /
• v.108 no.2
• /
• pp.269-275
• /
• 2019

The aim of our study was to compare the wound responses and fine root development after root cutting and wound dressing among different tree species. The roots (2~4 cm in diameter) of mature Pinus densiflora, Prunus serrulata var. spontanea, and Zelkova serrata grown in Sangju, South Korea, were cut every 6 months from May 2014 to November 2016. The cut roots were subjected to pastes of vaseline and organic fertilizer. Six months after cutting, fine root development in the cut roots was examined. There was a significant increase in the fine root development in P. serrulata and Z. serrata; however, the development was rarely observed in P. densiflora. Zelkova serrata showed the highest fine root development among the three species in both the control and organic paste treatment groups. No significant differences in fine root development were observed in P. serrulata among the treatments. These results indicate that different tree species exhibit different responses to wound dressing after root cutting, which suggests that specific treatments must be applied to specific tree species.

• Journal of Korean Society of Forest Science
• /
• v.105 no.3
• /
• pp.275-283
• /
• 2016

The objective of this study was to examine carbon dynamics with biomass, soil $CO_2$ efflux, litter and root decomposition after tree density control in young Pinus densiflora stands. The stands were established with 50% thinning, clear-cut, and control stands with three pseudo-replicated plots and a bare soil plot in 8-year-old Pinus densiflora nursery field. Monthly measurements were conducted from March 2012 to February 2014 and aboveground biomass and coarse-roots were estimated by derived allometric equations. Average diameter growth at root collar in control and thinned was 0.89 cm and 1.48 cm per year, respectively, and the diameter growth of control stand was significantly higher than that of thinned stands (p<0.05). Total biomass was estimated to 5.17, $4.85kg\;C\;m^{-2}$ per year in control and thinned, respectively. Annual soil $CO_2$ efflux in control, thinned, clear cut, and bare soil was 3.71, 3.90, 4.17, $4.56kg\;CO_2\;m^{-2}\;yr^{-1}$, respectively and removing trees significantly increased soil $CO_2$ efflux (p<0.05). Net Ecosystem Production (NEP) was 1.57, 1.36, -0.67, $-1.25kg\;C\;m^{-2}\;yr^{-1}$ in control, thinned, clear cut and bare soil in the young Pinus densiflora stands. NEP was significantly decreased by removing trees. Thinning increased diameter at root collar and carbon of individual tree and recovered 86% of carbon removed by thinning after one-year. In addition, soil $CO_2$ efflux increased and NEP increased by thinning. Results of this study, tree density control such as thinning increased the carbon storage and growth of the young Pinus densiflora stands.

• Korean Journal of Environment and Ecology
• /
• v.27 no.5
• /
• pp.571-578
• /
• 2013

This study generated regression models through a direct harvesting method to estimate carbon storage and uptake by Pinus densiflora and Pinus koraiensis, the major evergreen tree species in urban landscape, and established essential information to quantify carbon reduction by urban trees. Open-grown landscape tree individuals for each species were sampled reflecting various diameter sizes at a given interval. The study measured biomass for each part including the roots of sample trees to compute the total carbon storage per tree. Annual carbon uptake per tree was quantified by analyzing radial growth rates of stem samples at breast height. The study then derived a regression model easily applicable in estimating carbon storage and uptake per tree for the two species by using diameter at breast height (DBH) as an independent variable. All the regression models showed high fitness with $r^2$ values of higher than 0.98. While carbon storage and uptake by young trees tended to be greater for P. densiflora than for P. koraiensis in the same diameter sizes, those by mature trees with DBH sizes of larger than 20 cm showed results to the contrary due to a difference in growth rates. A tree of P. densiflora and P. koraiensis with DBH of 25 cm stored 115.6 kg and 130.0 kg of carbon, respectively, and annually sequestered 9.4 kg and 14.6 kg. The study has broken new grounds to overcome limitations of the past studies which quantified carbon reduction of the study species by substituting, due to a difficulty in direct cutting and root digging of landscape trees, coefficients from forest trees such as biomass expansion factors, ratios of below ground/above ground biomass, and diameter growth rates.

• Journal of Environmental Science International
• /
• v.28 no.8
• /
• pp.655-667
• /
• 2019

This research aims to identify the possibility of developing A horizon resources that can be used for construction and civil engineering work. As such, the utility of A horizon resources was examined by establishing planting ground through a mixture of soil layers and by analyzing the growth and development of Pinus densiflora. The physicochemical and physical properties of the soil were as follows: the A horizon was sandy clay loam, B horizon was sandy loam, and the mixture of two layers appeared as sandy loam, which was identical to the B horizon. The experimental groups did not show any significant difference in their physical properties of porosity and degree of water-stable aggregates. With regards to chemical properties, the A horizon as well as the mixture of A and B horizon showed acidity while the B horizon showed alkalinity. The figures of organic matter, total nitrogen, available phosphate, and replaceable potassium were greater as the A horizon content increased, whereas the figures of replaceable calcium, replaceable magnesium, and conductivity increased as the A horizon content decreased. As a result of the growth and development of Pinus densiflora in each planting ground, the final survival rates were all above 100%. However, the tree height and the rate of growth for the diameter of root were higher in the order of A horizon > A horizon + B horizon > B horizon,indicating that the increased A horizon content is related to the growth and development of Pinus densiflora. The treatment of soil with improvement agents, used to recover the functions of in-situ soil showing poor growth and development, did not have a clear impact on the soil texture and porosity. However, the degree of water-stable aggregates increased significantly when using O horizon as the soil improvement agent among the types of in-situ soil. In contrast, all items related to the chemical properties showed significant differences following the treatment by soil improvement agents. The survival rate according to the treatment of soil improvement agents for the growth and development of Pinus densiflora was higher in the order of organic horizon = no treatment > compound fertilizer > organic fertilizer + compound fertilizer > organic fertilizer; this result was statistically significant with a marginal significance value of the log-rank test(p < 0.05).

• Korean Journal of Environment and Ecology
• /
• v.25 no.1
• /
• pp.31-36
• /
• 2011

To provide the basic information about slope stability analysis, tensile force and strength of tree roots like Pinus koraiensis, Larix leptolepis, Pinus densiflora, Quercus mongolica, and Alnus japonica were measured and analyzed. As a result, tensile force increases in forms of involution of root diameter. The mean tensile strength of roots like P. koraiensis, L. leptolepis, P. densiflora, A. japonica and Q. mongolica were calculated as $165.38kgf/cm^2$, $172.78kgf/cm^2$, $176.25kgf/cm^2$, $214.29kgf/cm^2$ and $224.19kgf/cm^2$ respectively. It was shown that tensile strength decreasing tendency as root diameter increases. Also, recalculated soil shear strength by tensile strength of the roots like P. koraiensis, L. leptolepis, P. densiflora, A. japonica and Q. mongolica were $0.099kgf/cm^2$, $0.104kgf/cm^2$, $0.106kgf/cm^2$, $0.129kgf/cm^2$ and $0.135kgf/cm^2$ respectively.

• Journal of Korean Society of Forest Science
• /
• v.79 no.3
• /
• pp.322-329
• /
• 1990

A group of Pinus densiflora trees attacked by Rhizina root rot were observed at Kangnung. Diseased roots are characterized by rot patches, radial rot traces or formation of adhesive soil masses. The damage has proceeded about 6m per annum, and the pathogen in the infected soil was detected by trap logs. Ascospores of Rhizina undulata was germinated by heat shock at $37^{\circ}C$ for 24 hours or at $40^{\circ}C$ for 17 hours. The mycerial growth was optimum on PDA medium at $25-30^{\circ}C$and pH 5.6-6.3. Coniferous trees were more susceptible than non-coniferous trees in inoculation test in vitro.

• Journal of Korean Society of Forest Science
• /
• v.89 no.3
• /
• pp.389-396
• /
• 2000

To determine whether the Tricholoma matsutake (pine mushroom, Songyi) is symbiotic or parasitic to Pinus densiflora, structural change in their natural ectomycorrhizas were examined. The mycorrhizal samples were collected at three progressional points in the natural hypogeous colony(shiro) : colony front edge, near the fruiting point and 20cm back. The fine roots in the colonies were typical ectomycorrhizas with fungal mantle and Hartig net. However, the T. matsutake mycorrhizas had unique characteristics compared to other types of ectomycorrhizas. That is, spatially the fungal mantle and Hartig net of the T. matsutake mycorrhizas continued to develop along the growing tip, while temporally those structures declined to shrink changing to black brown in the older part of the roots behind the actively growing tip portion. However, there was no mark that the fungal hyphae penetrated into either the cortical cells, endodermal cell layers or stele. The apical tips of the blackened roots remained alive to form new mycorrhizas with other fungi later. Therefore, we conclude that the mycorrhiza of T. matsutake+P. densiflora is rather a dynamic symbiosis that changes its position spatiotemporally as the root grows than either a simple parasitism or symbiosis.

• Journal of Applied Biological Chemistry
• /
• v.61 no.3
• /
• pp.275-281
• /
• 2018

Pinus densiflora root (PDR) is used as a medicinal plant. In this study, we investigated whether the PDR extract has anti-inflammatory activities. Cell viability assays showed that the extract was not toxic toward RAW 264.7 cells at concentrations up to $10{\mu}g/mL$. At $10{\mu}g/mL$, the extract decreased nitric oxide (NO) content to 40% of the control level. The protein expression of inducible nitric oxide synthase (iNOS), which generates NO, decreased with increasing concentrations of the extract. Prostaglandin $E_2$ ($PGE_2$) levels were significantly inhibited by over 50% in the presence of $10{\mu}g/mL$ of the extract. The protein expression of cyclooxygenase-2 (COX-2), which generates $PGE_2$, decreased with increasing concentrations of the extract. Proinflammatory cytokines, such as tumor necrosis factor-alpha ($TNF-{\alpha}$), interleukin-6 (IL-6), and $IL-1{\beta}$, were detected in RAW 264.7 cells after lipopolysaccharide (LPS) treatment. The extract did not affect the levels of $TNF-{\alpha}$ and IL-6, but it significantly inhibited the level of $IL-1{\beta}$. It also completely inhibited the transcription of nuclear factor-kappaB ($NF-{\kappa}B$). These results indicate that the PDR extract reduces inflammatory response-related proteins, such as NO, $PGE_2$, iNOS, and COX-2, in LPS-induced RAW 264.7 cells via the regulation of $NF-{\kappa}B$. Consequently, we have provided a mechanism to explain the anti-inflammatory effect of the PDR extract; that is, it exerts such an effect by regulating $NF-{\kappa}B$. The PDR extract can therefore be considered as an effective anti-inflammatory agent.

• The Korean Journal of Mycology
• /
• v.44 no.3
• /
• pp.155-165
• /
• 2016

Domestic and international production of Tricholoma matsutake has decreased owing to matsutake forests being left alone, host plant disease, forest fires, climate change, and so on. In order to identify strains that are suitable for the production of T. matsutake-inoculated seedlings, Pinus densiflora seedlings were inoculated with T. matsutake after in vitro rooting and mycorrhization was examined in the roots of T. matsutake-inoculated seedlings after 6 months. The mycorrhization rate was greater than 80% for 5 strains (NIFoS 421, 434, 1681, 1984, and 2001) out of 19 total strains. Seven strains (NIFoS 434, 441, 561, 562, 1016, 1807, and 1812) showed shoot/root ratios of less than 3.0 and had a seedling shoot biomass of 2.0 to 4.8 times higher than that of the root. Eight strains (NIFoS 441, 561, 562, 1016, 1807, 1812, 1984, and 2001) stimulated increases in shoot volume and three stains (NIFoS 441, 562, and 1812) promoted the growth of root biomass by mycorrhizal formation. In conclusion, 4 strains (NIFoS 434, 561, 1984, and 2001) out of 19 total strains tested showed higher mycorrhization rates and seedling growth than those of the other strains. We expect that the use of these four strains may contribute to T. matsutake-inoculated seedling production.