• Journal of Korean Medical classics
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• v.27 no.1
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• pp.15-28
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• 2014

Objective : Jeol-sik, (festive seasons every 15 days based on sun cycle), refers to both Korean Traditional Festival food(jeol-sik), intertwined between months, and in-season meals(si-jeol-sik) in which the ingredients used are produced in each and one of the four season. I found that ingredients in Si-jeol-sik of Autumn are helpful for strengthening one's life force. Method : The study found that si-jeol sik for fall season has strong characteristic of casting out demons. When making songpyeon(half-moon-shaped rice cake) for Chuseok (Korean Thanksgiviing Day), they are steamed with pine needs spreaded underneath because pine needles are believed to have power in casting away evil spirits. For harvest ceremony, which were frequently held in October, people consumed steamed rice cakes garnished with red beans, which were considered to negate bad fortunes. Result : To prevent respiratory diseases caught esaily in fall due to wide daily temperature difference and dry weather, white good ingredients such as radish, pear, and taro are used. Conclusion : Main fall holidays are Chil-soek, Baek-Jung, Chu-seok. The best feature of Si-jeol-sik for fall season is that they cast out demons. Also, fall si-jeol-sik consist of many white ingredients for protecting lungs.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.4
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• pp.691-695
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• 2002

This study was performed to examine the cytotoxic effects of the distilled pine-needle extracts against several cancer cell lines. First, cell lines including mice leukemic cancer cell line (L1210), sarcoma 180 and human monocyte-like cancer cells (U937) were tested using XTT methods in uitro. Pine-needle extracts were prepared by pressing the pine needles and distilling it at below 98$^{\circ}C$ and then added to the growth medium in a final dilution of 10, 20, and 40 times. Growth of three kinds of cancer cells was significantly inhibited by more than 50% with the addition of the extracts. Fifty six to seventy six % of inhibition was shown with the 40 times dilution of the extracts. Greater inhibition was achieved with the 20 times dilution (81~90%) and the 10 times dilution (77~89%) of the extracts. Next, other human cancer cell lines including 3 kinds of breast cancer cell lines (T47D, MDA-MB-231 and MW7A) and one hepatoma cell line (SNU-354) were tested with the 20 times dilution of the extract. T47D and MDA-MB-231 cell lines showed lower inhibition (12%) with the addition of the extract. However, MH7A and SNU-354 cell lines showed 64% and 72% inhibition with the extract, respectively. These results suggest that the distilled pine-needle extracts have strong cytotoxic effect on certain cancer cell lines and the intensity of the effect may vary depending on the process of the pine needle.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.38 no.1
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• pp.45-52
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• 2020

This study analyzed the components contained in the pine needles of first and second-year-olds to analyze the factors that the in vivo content of inorganic elements affects the sex determination of pine trees. In response, the plan for pine tree plant and maintenance was intended to be presented in consideration of the reproductive environment and physiological characteristics. The results are as follows. First, last year, when there were many encyclopedias, the analyzed N(%) content was found to be high. The nitrogen content of the previous year's soil was found to affect the production of the spheres the following year. This is believed to be possible to reduce the rate of Pine pollen produced in the new plant in the following year through a dispute over quality consumption in the spring of the previous year. Second, the weapons elements involved in the Pine cones and the generation of the Pine pollen in the new plant appeared to be P(%), K(%), Ca(%), and Fe(%). However, the nutrients from the previous year's leaves of Ca(%) and Fe(%) were found to have a low influence on the sex determination of first-year pine trees. Because Ca(%) and Fe(%) are not able to move nutrients accumulated in aging organs due to the nature of the components, feeding nutrients in the fall when the growth of the previous year's branches is reduced is expected to affect oral generation. Third, pine trees are extremely positive and Pine pollen is related to the time of the northeast wind. Therefore, it is believed that it would be good to be located in the northern direction, where the sunlight is good, in an outdoor space. In addition, it is important to use vaginal consumer products in spring and carry out a quarrel involving Mg and Fe during fall to reduce the effect of the Pine pollen, which is an outdoor plant. This is an important part of maintaining and managing pine trees in outdoor spaces as well as the sex determination of pine trees. This study suggested that plant planning, which derives the correlation between pine inorganic element content on sexual determination and takes into account the physiological characteristics of pine trees, is an important issue in the creation of outdoor space. Follow-up research on other factors affecting pine tree sex determination is expected.

• Journal of Nutrition and Health
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• v.35 no.3
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• pp.291-295
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• 2002

This study was designed to investigate the effects of a butanol (BuOH) fraction from an extract of pine (Pinus densiflora Sieb et Zucc.) needles, on oxygen radicals and their scavenger enzymes in the liver membranes of rats. Twenty-eight male Sprague-Dawley (SD) rats were divided into four groups over a 45 days study period: the control group on a basic diet, and three experimental groups on three different dietary levels of the butanol fraction, specifically 25 mg (BuOH-25), 50 mg (BuOH-50), and 100 mg (BuOH-100) butanol fraction/kg body weight/day, thereby 0.025%, 0.05%, 0.1% of butanol extract of pine needles was added to basil diet respectively. At the end of the experimental period, body weights and food intakes were not different among the four groups. The results showed that cholesterol accumulation in the mitochondria and microsomes of liver cells was significantly inhibited in the BuOH-50 and BuOH-100 groups: by 11.6% and 20.1% in the mitochondria of the BuOH-50 and BuOH-100 groups, respectively; and by 10.5%, and 13.5% in the microsomes of the BuOH-50 and BuOH-100 groups, respectively, compared with the control group. The levels of hydroxyl radicals (.OH) were significantly) lower in the liver mitochondria of the BuOH-50 and BuOH-100 groups (by 13.3% and 18.5%, respectively), while OH radicals were significantly lower in the microsomes or all three experimental groups (by 15.7% in the BuOH-25 group, 20.0% in the BuOH-50 group, and 20.6% in the BuOH-100group), compared with the control group. Superoxide radical (O$_2$) formation was also significantly inhibited in the liver cytosol of both BuOH-50 and BuOH-100 groups; the levels of these radicals were 8.0% lower for the BuOH-50 group and 11.1% lower for the BuOH-100 group, compared to the control group. Copper/Zinc - superoxide dismutase (Cu/Zn-SOD) activities were significantly increased (by 10.3% and 15.9%, respectively) in the liver cytosols of the BuOH-50 and BuOH-100 groups, but Mn-SOD activities were almost identical in the three RuOH groups, compared with the control group. Glutathione peroxidase (GPx) activities were significantly increased in the three experimental groups (by 9.0% in the BuOH-25 group, 19.4% in the BuOH-50 group, and by 25.6% in the BuOH-100 group), compared with the control group. These results suggest that the butanol extract of pine needles may play an effective role in attenuating oxygen radicals and activating scavenger enzymes; consequently, aging may be very effectively modulated and/or inhibited.

• Journal of Nutrition and Health
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• v.35 no.3
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• pp.296-302
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• 2002

This study was designed to investigate the effects of a butanol extract of pine needles (Pinus densiflora Sieb et Zucc) on lipid metabolism and oxidative stress in rats. Twenty-eight male Sprague-Dawley (SD) rats were divided into four groups over a 45 days study period: the control group on a basic diet, and three experimental groups on three different dietary levels of the butanol fraction, specifically 25 mg (BuOH-25), 50 mg (BuOH-50), and 100 mg (BuOH-100) butanol fraction/kg body weight/day, thereby 0.025%, 0.05%, 0.1% of butanol extract of pine needles was added to basic diet respectively. At the end of the experimental period, body weights and food intakes were food intakes were not different among the four groups. Total and LDL-cholesterol levels were markedly decreased in the BuOH-25, BuOH-50, and BuOH-100 groups, respectively, as follows: 12.8%, 19.1% and 21.6% reductions in total cholesterol; and 10.2%, 15.6% and 23.7% reductions in LDL-cholesterol. However, HDL-cholesterol levels were significantly increased (by approximately 20%) in the serum of the BuOH-100 group only, compared with the control and other experimental groups. Atherogenic indices were also markedly decreased in the three experimental groups, by 24.8%, 30.4% and 36.2%, for each of the BuOH-25, BuOH-50, and BuOH-100 groups, respectively, compared with the control group. The levels of the hydroxyl radical (·OH) and of lipid peroxide (LPO) in the serum of the three experimental groups were significantly reduced, by 9.8%, 19.7% and 21.2%; and by 13.3%, 13.3% and 16.7%, for the BuOH-25, BuOH-50, and BuOH-100 groups, respectively Significant increases in serum superoxide dismutase (SOD) were observed in the BuOH-50 and BuOH-100 groups; specifically, 12.1% in the BuOH-50 group and 23.3% in the BuOH-100 group, compared with the control group. Significant increases in catalase (CAT) avtivities, of 24.7% in the BuOH-50 group and 29.2% in the BuOH-100 group, were also observed, compared to the control group. These results suggest that a butanol extract of pine needles could inhibit chronic degenerative disease through improving lipid metabolism, and could also effectively modulate the aging process attenuating oxidative stress.

• Journal of Korean Society of Forest Science
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• v.88 no.1
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• pp.31-37
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• 1999

The frequency of gall formation by pine needle gall midges ranged from 35% to 40% in both P. densiflora and P. thunbergii species. However, there was no indication of gall development from larva on both P. virgiana and P. rigida species suggesting that some specific compounds might play role in preventing gall formation of larva. The susceptible species to pine needle gall midges including P. densiflora and P. thunbergii contained a little salicylic acid under the free phenolic compound conditions while the resistant species against such insects including P. virginiana and P. rigida species contained about 37ppm to 50ppm of salicylic acid. Thus, this compound might have important roles in insect resistance. The contents of internal salicylic acid in the needles of susceptible pines increased from 9.5ppm to 20.6ppm after direct external irrigation of salicylic acid solution and flour treatment on roots. As a result, the frequency of gall formation decreased dramatically 17~19 times lower when compared with that of control. According to our results, the application of salicylic acid for effective prevention against insect damages should be performed before hatching eggs. The frequency of gall formation in the hybrid pines of P. thunbergii(susceptible) and P. virginiana(resistant) showed approximately 24% as average value of those in both species. In the case of specific individuals, it ranged from 2.8% to 11.5% in hybrids of both species. Therefore, effective production of insect resistant pines can be obtained through hybrids by crossing between susceptible and resistant species including P. thunbergii and P. virginiana by increasing internal salicylic acid contents of the needles.

• Journal of Korean Society of Forest Science
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• v.99 no.1
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• pp.52-56
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• 2010

Crown fuel characteristics such as crown bulk density, crown base height, and fuel moisture content of Japanese red pine were analyzed. Ten trees in Mt. Palgong at Daegu, were destructively sampled and their crown fuels were weighed separately for each fuel category. Fuel content of live and dead crown component were 53%, and 15.3%, respectively. Foliar moisture content was 56%. Needles and twigs with diameter less than 1cm diameter accounted for 16.2%, 55% of total and crown fuel load. Average crown bulk density of Japanese red pine was 0.24 kg/$m^3$, effective crown fuel bulk density was 0.1325 kg/$m^3$.

• Journal of Korean Society of Forest Science
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• v.73 no.1
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• pp.43-54
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• 1986

This study was carried out to investigate the influence of light intensity on the growth of Korean white pine (Pinus koraiensis) seedlings. The seedlings were grown under four different relative light intensities: 100%, 63%, 37%, and 19% of full sunlight by covering with saran screen on seed bed. The results obtained were summarized as follows; 1. The highest shoot elongation was found at 37% in relative light intensity (RLI) plot, and the best growth in root, seedling length and diameter increment appeared at 100% on RLI plot. Contribution rates of shading to growth in shoot elongation, root growth, seedling length, and diameter increment were 9.3%, 26.2%, 21.0%, and 48.7%, respectively. 2. The greatest number of one-year-old needle fascicle was found at 100% in RLI plot, whereas that of two-year-old needle fascicle appeared at 37% in RLI plot. The contribution rate of the light intensity to number of needle fascicle was 3.9% and factor of leaf age contributed to number of needle fascicle in 27.8%. The length of needles grown under different light intensities varied with needle ages. The longest length of new needles appeared at 37% in RLI plot, but old needles were not clear in influence of light intensity. The contribution rate to needle length by the light intensity was 2.5%. 3. The heaviest value of the fresh and dry weight of seedlings appeared at 100% of RLI plot then decreased with light intensity. The contribution rates to fresh and dry weight by the shading were 38% and 7.6% respectively. 4. The largest value of the dry weight of needles appeared at 100% of RLI plot and then decreased with light intensity. The contribution rates to dry weight of needles by the light intensity was 13.18%. 5. The values of T/R ratio increased with decreased light intensity and the contribution rate was 7.0%. 6. Positive correlation and linear regressions were recognized between dry weight of leaf and other factors (dry weight of shoot, root, seedling and diameter increment).

• Journal of agriculture & life science
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• v.45 no.2
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• pp.93-101
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• 2011

This study was conducted to investigate the effect of probiotic from pine needle microbes on growth performance, blood characteristics, carcass traits and economy of pigs. One hundred eighty pigs (L${\times}$Y${\times}$D, average body weight=75 kg) were grouped and housed with 15 animals per pen for 3 replications per each treatment. The ingredients and pine needle microbes mixed and fermented on anaerobic condition for 10 days to make the fermented diet. The basal diet was substituted with 0 (CON), 3 (T1), 5 (T2) and 10% (T3) of fermented diet, the pigs were fed for 42 days and slaughtered at the end of the trial to measure the carcass traits. Crude protein concentration of fermented diet on 10 day was higher (p<0.05) than that of on 0 day, while crude fiber concentration and pH were lower (p<0.05). The supplementation of fermented diet did not affect on growth performance and feed intake. Hemoglobin concentration of whole blood in T3 was higher (p<0.05) than that of CON, and hematocrit concentrations in CON and T1 were higher (p<0.05) than that of T2 and T3. The concentration of platelet was higher (p<0.05) in T1 than in T2 and T3. The CON had higher (p<0.05) total cholesterol, LDL-cholesterol and triglycerides concentrations than the treatments, whereas lower (p<0.05) HDL-cholesterol concentration. Carcass weight and dressing in T1 and T3 were higher (p<0.05) than those of CON and T2. The backfat thickness was higher (p<0.05) in CON and T2 than in T3. The carcass grade and economy improved by supplementation of fermented diet compare to CON. In conclusion, the supplementation of fermented diet using probiotics from pine needle microbes could improve health and meat quality and reduce the feed cost of pigs.

• Journal of Korean Society of Forest Science
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• v.99 no.3
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• pp.391-396
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• 2010

To analyze the characteristics of canopy fuel in Pinus densiflora stand, which is essential to assess the crown fire hazard, allometric equations for estimation of crown fuel biomass were developed by subjectively categorized crown fuel component and crown bulk density was analyzed by available fuel component categories. Ten trees were destructively sampled at Pinus densiflora stand in Mt. Palgong in Daegu and their crown fuels were weighed separately for each fuel category by size classes and by living and dead. Regression equations that estimate crown fuel load by diameter at breast height(D) or additional total height(H) were derived. The adjusted coefficient of determination values were the highest (${R^2}_{adj}$=0.835-0.996) and standard error of estimate were the lowest (SEE=0.074-0.638) in the allometric equation lnWt=${\alpha}+{\beta}lnD+{\gamma}lnH$ in average. However, in needles and small branches categories, the differences in ${R^2}_{adj}$ and SEE between equations were not significant. Crown bulk density (CBD), which was calculated by crown fuel load divided by crown volume, was 0.067 kg/$m^3$ in average when only needles were considered as available crown fuel and 0.097 kg/$m^3$ when needles and branches (0-0.5 cm diameter) were considered. The increments of CBD of needles and small branches were little even when diameter at breast height increased.