• Journal of Species Research
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• v.12 no.3
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• pp.229-239
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• 2023

We isolated and identified 25 unrecorded bacterial species belonging to the phylum Actinomycetota found in the Republic of Korea. Sequence comparison of 16S rRNA was performed using the NCBI BLAST and EzBioCloud database to identify 25 species, which had a 16S rRNA gene sequence similarity of >98.8% and were allocated as unrecorded species in the Republic of Korea. Among the 25 unrecorded bacterial strains, Streptomyces was the most common with nine species, followed by Leifsonia with two species. Isoptericola, Nocardioides, Dermacoccus, Sinomonas, Patulibacter, Marmoricola, Allobranchiibius, Aldersonia, Actinokineospora, Agromyces, Aeromicrobium, Cellulomonas, and Gordonia with one species each were also found. Twenty-five unrecorded species were excavated in various environments, such as tidal flats, ferns, soil, pine cones, moss, mud, wetlands, and plants. These isolates were characterized on the basis of their phylogenetic, biochemical properties, and morphological data, and species descriptions were provided.

• Journal of Korean Society of Forest Science
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• v.77 no.1
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• pp.92-99
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• 1988

The dark respiration, photosynthesis($CO_2$ refixation), $CO_2$ balance and chlorophyll content of 1st-year conelets and 2nd-year cones of Korean pine(Pinus koraiensis S.et.Z.) were investigated after pollination up to the end of maturation. The results obtained are as follows : 1. The growth of 1st-year conelet was 3.6cm in length. 2.4cm in diameter and 3.058 in dry weighs during the first year. The growth of 2nd-year mature cone was 13.5cm in length, 9.3cm in diameter and 141.0g in dry weight in the late of 2nd-year. 2. The refixation of carbon dioxide released from a cone by the dark respiration was less than 50 percent at light saturation through the growing period. The refixation of carbon dioxide released by dark respiration for one year was 7.3 percent in 1st-year conelets and 8.7 percent in 2nd-year cones. 3. The dark respiration rate of cones by increasing temperature was rapidly increased with increasing temperature up to $25^{\circ}C$. The dark respiration rate of cones was much higher in non-growing season than that in growing season at the same temperature. 4. The rates of dark respiration and $CO_2$ refixation, based on the dry weight, were much higher in 1st-year conelet than that in 2nd-year cone. 5. The $CO_2$ balance for a cone was negative from pollination to the end of maturation. The net dark respiration loss for a cone was 7.23g $CO_2$/year in 1st-year conelet and 164.8g $CO_2$/year in 2nd-year cone. 6. The respiratory loss efficiency for a cone(=$CH_2O$ weight calculated by net dark respiration/dry weight of cone) for one year was 1.61 in 1st-year conelet and 0.81 in 2nd-year cone for one year. 7. The total chlorophyll content of surface scale of the cone was lower than 2mg/g dw through the growing period, and chl. a/b ratio was 2 to 3.

• Journal of Korean Society of Forest Science
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• v.83 no.4
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• pp.498-504
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• 1994

The damage by the black-tipped sawfly, Acantholyda posticalis posticalis Matsumura, has been incheasing at several locations in central part of the Korean peninsula. This study was undertaken at selected plots in locality of Kapyung-gun. Kyunggi Province from 1984 through 1987. The purpose of this study was to provide basic information for integrated control of this pest and to determine the economic threshold by investigating the pest-host interactions with special reference to damage analysis. The results obtained were as follows ; 1. One year old cone formation began to be reduced when 30-40% of the needles were destroyed. With 70% of defoliation, few cones were produced. 2. Number of two year old cone formation and amount of cones began to be reduced when 61-70% of the needles were destroyed ; economic threshold of the defoliation rate retarding the seed production was 51%. 3. Growth of the trees was significantly reduced when more than 50% of the needles were destroyed, and cone production began to be retarded at 30-40% of defoliation. Therefore, it was determined that the economic thresholds requiring the control practices to keep normal tree growth and cone production from being reduced were 50% and 30%, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.12
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• pp.1734-1741
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• 2016

This study was conducted to examine the effects of adding phytoncide oil extracted from Korean pine nut cone by-product to the diet of dairy cows on milk yield and compositions, fatty acid characteristics, complete blood count and stress response. A total of 74 Holstein cows were used for 30 days and divided into two groups. Each group was given a basal diet (C) or an experimental diet containing phytoncide additives at 0.016% (T) in feed. The results showed that phytoncide feeding had no effect on milk yield. In addition, there were no observed effects on milk composition, but the ratio of fatty acid in milk was significantly affected by the phytoncide diet, and it showed a positive effect. Not only were the major functional fatty acids, conjugated linoleic acid and eicosapentaenoic acid increased, but also ${\omega}6:{\omega}3$ fatty acid ratio was reduced in milk of T group (p<0.05). In blood analysis, the complete blood count showed no significant difference between C and T group on all parameters. However, the cortisol concentration was significantly decreased in T group compared to control (p<0.05). Taken together, we suggest that phytoncide oil does not have a great influence on the physiological changes, but can be a potential feed additive that improves the milk fatty acid and stress resilience in dairy cows. In addition, it will contribute to the development of feed resource, a reduction in feed cost and a lessening of environmental pollution.

• Korean Journal of Plant Resources
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• v.28 no.2
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• pp.253-258
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• 2015

In South Korea, Pinus pumila (Pall.) Regel (dwarf stone pine) has been designated as a critically endangered species by the Korea Forest Service. We have difficulties in obtaining the seeds of P. pumila because P. pumila grows only in the Daecheongbong area (1550–1700 m above sea level) of Mt. Seorak and almost all of its cones are damaged by birds and rodents. For establishing an ex situ conservation stand of P. pumila, this study was conducted to figure out the effects of wet cold (cold stratification, prechilling) and GA₃ treatment on the germination of P. pumila seeds. After cold stratification (1, 2, 3, 4, 5 months), prechilling (1, 2, 3, 4, 5 months) and GA₃ treatment (0, 100, 500, 1,000, 2,000, 3,000 ㎎/L), seeds were placed on petri-dishes at 25℃ under light condition. The percentage of germination, mean germination time and the germination rate were investigated. The results showed that both of the cold stratification and prechilling were effective in improving germination performances. However, there were no significant differences in performances between the two cold treatments. Within each treatment, the germination performances improved with the period of treatment. However, after three months of treatment, the performances showed no significant improvement. The gibberellin treatment was also effective in improving seed germination of P. pumila. The percentage of germination reached 79.0% in the seeds treated with 100 ㎎/L of GA₃. However, the germination performances decreased at high concentration of GA₃ treatments (over 2000 ㎎/L). In conclusion, cold stratification (over 3 months) or 100 ㎎/L of GA₃ treatment was considered to be the appropriate method for seedling production of P. pumila.

• Korean Journal of Plant Taxonomy
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• v.48 no.1
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• pp.43-47
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• 2018

Pimpinella saxifraga L. (Scarlet pimpernel or Burnet saxifrage), a newly introduced species, was found from Baekyakyi oreum (a parasitic cone or cinder cone) in Seogwipo, Jejudo Island. This species is native to Europe and western Asia and is reported as a widely invasive species around the world. Pimpinella saxifraga is distinguished from other related Korean taxa by leaves that are pinnately compound with 3-7 pairs of segments, which vary from simple, ovate, to 2-pinnatisect, with linear lobes. The vernacular name of "Baek-yak-yi-cham-na-mul" was assigned after the name of the cinder cone where it was initially discovered. We provide descriptions of the morphological characters, photographs and a key to allied taxa in Korea. The geographical origin and introduction routes are unknown, but it is highly likely that it was introduced as fodder given its high nutritious value for sheep and cattle or as a medicinal herb. This species invades commonly grasslands and woodlands and prefers dry, well-drained, and calcareous soils. The distribution of this species was analyzed by searching extensively in surrounding Baekyakyi oreum and adjacent cinder cones, but no other populations were observed. It is protected commonly at the edges or inside of the patchy pine forest in the cinder cone from grazing by cattle; given the worldwide invasive nature of this species, special monitoring and management plans should be developed.

• Korean Journal of Agricultural and Forest Meteorology
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• v.12 no.3
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• pp.207-216
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• 2010

Individual differences of ozone ($O_3$) resistance for seed production, seed germination and seedling development were examined in this study. Five in each healthy and damaged trees of Pinus thunbergii growing in air polluted area for 12 years were chosen based on visible foliar injury and growth. The cones of P. thunbergii, which were collected from healthy and damaged trees, were analyzed for physical characteristics and seeds from the cones were used to test germination percentage under $O_3$ treatment. The germinated seeds were continuously exposed to $O_3$ treatment and the lipid peroxidation and activities of antioxidative enzymes were determined for both seeds and seedlings. The $O_3$ treatment for seed germination and seedling development were conducted at three conditions: control, 150 ppb and 300 ppb of $O_3$. The non-treated seeds from the damaged trees showed 21.6% lower germination than those from the healthy ones. On the $O_3$ treatment of 300 ppb, seed germination decreased approximately 10% for the healthy trees and 19% for the damaged trees compared to that on the control. The seeds from the healthy trees showed significantly higher activities of superoxide dismutase (SOD), glutathione reductase (GR), and catalase (CAT) than those from the damaged trees. The activities of GR, ascorbate peroxidase (APX), and CAT decreased along with the increasing $O_3$ concentration in two tree grades. Malondialdehyde (MDA) content of seeds was not influenced by $O_3$ treatment for two tree grades. In seedling development, there were no significant differences for length and biomass of needle and root of two tree grades at both the control and 150 ppb of $O_3$. At 300 ppb of $O_3$ treatment, however, the length and biomass of needle and stem decreased for two tree grades but no significant differences was detected in root. The seedlings from the damaged trees were more sensitive to the $O_3$ treatment, showing higher activities of SOD, APX, and CAT and content of MDA compared to those from the healthy tree seedlings. Our results indicate that seed germination and seedling development are vulnerable to increasing $O_3$ concentrations and that attention must be paid to the individual selection of tree species for reforestation.

• Journal of Korean Society of Forest Science
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• v.88 no.2
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• pp.195-204
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• 1999

To improve nut production and timber quality in a plantation, crown shape controls were suggested for Korean white pines (Pinus koraiensis) under age class III through the analyses of the crown shape controls for seed orchard trees in several species, of crown status of controlled and uncontrolled trees in a Korean white pine seed orchard, and of growth characteristics of plantation trees under age class III. For nut production from the small trees less than 8 m, modified leader type can be applied by cutting the central leader and all branches below 1 m and keeping 4~5 nodes with 3~4 whorled branches a node locating 1 m apart. Trees with a 4~8 m long trunk below the first living branch are shaped into trees for nut and timber production, which have a 4~8 m long knotless stem and a modified leader type crown with 4~5 nodes with 3~4 whorled branches a node at a distance of 1 m. Trees, showing the first living branch at or above 9 m from the surface, are pruned severely for timber production and result in a central leader type containing two fifths of the initial crown. It is advisable to remove the leader and the branches when collecting cones or after/during October to take advantage of easy labor and little resin while cutting. After stem cut, branches showing apical dominance are to be cut to control height when necessary. The removal of the uppermost node may be needed to control the crown shape several years after the initial stem-cut.

• Journal of Korean Society of Forest Science
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• v.62 no.1
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• pp.82-86
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• 1983

Cone and seed development after self-pollination in 14-year-old Pinus rigida Mill. was investigated in an $F_1$-hybrid seed orchard that consists of Pinus rigida Mill. and Pinus taeda L. in alternative rows. On an average, 83.8% of the self-pollinated receptive female strobili developed into cones. This value appears to be normal compared to cone development after open-pollination in other pine species such as in Pinus sylvestris L. However, there was a great variation in filled seed development after self-pollination among the investigated individual trees (Table 1-1). The 34.5%, a ratio of filled seeds after self-pollination was significantly lower than 91.7%, that by the open-pollination. Some of the investigated trees developed 70.0%-83.5% of filled seeds after self-pollination. Thus, those are not desirable as trees for seed parent in establishing $F_1$-hybrid seed orchards in future. A further study on the rate of filled seed development by various pollination systems consisting of self-pollination, self-and cross-pollination with intraspecific, mixed (tree's own and foreign pollens) pollens and cross-pollination with interspecific, mixed pollens of the two parental tree species is necessary to determine the quality of seed parent trees in establishing $F_1$-hybrid seed orchards.

• Korean Journal of Environment and Ecology
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• v.32 no.1
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• pp.23-29
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• 2018

The litter production, the nutrient concentration of each component of litterfall, and the amounts of nutrient into the forest floor via litterfall were assessed for 5 years from January 2009 through December 2013 in a Pinus densiflora forest in Mt. Worak National Park. The average amounts of leaf litter, branch and bark, reproductive organs(flowers and cones), and miscellaneous categories for 5 years were $1.940{\pm}0.21$, $0.505{\pm}0.15$, $0.259{\pm}0.09$, and $0.737{\pm}0.14t\;ha^{-1}yr^{-1}$, respectively. The average percentage of leaf litter, branch and bark, reproductive organs and miscellaneous categories for 5 years were 56.4, 14.7, 7.5, and 21.4%, respectively. The amounts of total litterfall in 2009, 2010, 2011, 2012, and 2013 were 2.810, 3.796, 3.268, 3.284, and $4.045ton\;ha^{-1}yr^{-1}$, respectively. The average amounts of litterfall for 5 years were $3.441{\pm}0.4ton\;ha^{-1}yr^{-1}$. The average amounts of N, P, K, Ca, and Mg returned to the forest floor via litterfall for 5 years in this Pinus densiflora forest were $22.73{\pm}4.92$, $1.05{\pm}0.42$, $4.26{\pm}1.69$, $8.48{\pm}4.62$, and $2.42{\pm}1.01kg\;ha^{-1}yr^{-1}$, respectively.