• Journal of Korean Society of Forest Science
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• v.87 no.4
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• pp.543-548
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• 1998

Portions of chloroplast genes(psbD and rbcL) were amplified from Pinus thunbergii(Japanese black pine : black pine) and Pinus densiflora(Japanese red pine : red pine) by PCR and digested by a restriction enzyme, HaeIII, respectively. Two species specific cpDNA markers were identified. With the observed cpDNA markers, paternal inheritance of cpDNA in pine hybrids was verified in an artificial hybrid family between black pine(Chollanam 37) and red pine(Chungchongbuk 3). On the basis of paternal inheritance of chloroplast genome in a hybrid, 2 portions of cpDNA amplified from 115 individuals of Pinus densiflora for. erecta were screened to detect any traces of black pine specific cpDNA markers in P. densiflora for. erecta which has been postulated as an introgressive hybrid between red pine and black pine(Hyun el al., 1967). All the analyzed individuals of Pinus densiflora for. erects revealed the identical profiles of HaeIII digested psbD and rbcL genes to red pine. This result suggests that there is no introduced chloroplast genome of black pine in Pinus densiflora for. erecta and that there is no concrete evidence of treating P. densiflora for, erecta as an introgressive hybrid between red pine(♀) and black pine(♂).

• The Korean Journal of Ecology
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• v.25 no.2
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• pp.127-134
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• 2002

This study was carried out to analyze Pinus densiflora forest in islets of southwestern coast of the Korean peninsula by the phytosociological methods. As a result of the investigation of 26 quadrats, the pine forest was classified two communities and six subcommunities : Ardisia japonica - Pinus densiflora community and lsodon japonicus - Pinus densiflora community, and Farfugium japonicum subcommunity, Quercus serrata subcommunity, Typical subcommunity of Ardisia japonica - P. densiflora community, Juniperus rigida subcommunity, Pourthiaea villosa subcommunity and Typical subcommunity of lsodon japonicus - P. densiflora community. The subsidiary knowledges from this study provide practical information to clarify the syntaxonomy of natural pine forest of the Korean peninsula by the phytosociological methods.

• Korean Journal of Plant Resources
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• v.32 no.4
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• pp.275-281
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• 2019

The pathological condition of excessive melanogenesis causing freckles, melasma, senile lentigo, pigmented acne scars, and cancer has a critical impact on the wellness of individuals. The mechanism of melanogenesis is related to the expression of melanogenic enzymes. Here, we evaluated the inhibitory effect of pine cone (Pinus densiflora) extracts on melanogenesis. P. densiflora, the Korean Red Pine, is the predominant tree species in the cool, temperate forests of northeast Asia, occurring in pure stands across Korea, Japan, and parts of northern China and Russia. P. densiflora leaves, pollen, and bark have been widely used for traditional medicine, or edible purposes. However, pine cones are rarely used as natural raw materials, although they contain many bioactive phytochemicals. The pine cone ethyl acetate fraction (PEF) showed no toxicity to B16F10 cells at a concentration of less than $100{\mu}g/mL$. PEF inhibited the expression of microphthalmiaassociated transcription factor (MITF), tyrosinase and tyrosinase-related factors in B16F10 cells treated with 3-Isobutyl1-methylxanthine (IBMX). These results suggest that pine cones can be used as an effective natural melanogenesis inhibitory agent.

• The Korean Journal of Ecology
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• v.25 no.3 s.107
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• pp.197-204
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• 2002

This study was carried out to analyze Pinus densiflora forest in islets of southwestern coast of the Korean peninsula by the phytosociological methods. As a result of the investigation of 26 quadrats, the pine forest was classified two communities and six subcommunities : Ardisia japonica-Pinus densiflora community and Isodon japonicus-Pinus densiflora community, and Farfugium japonicum subcommunity, Quercus serrata subcommunity, Typical subcommunity of Ardisia japonica-P. densiflora community, Juniperus rigida subcommunity, Pourthiaea villosa subcommunity and Typical subcommunity of Isodon japonicus-P. densiflora community. The subsidiary knowledges from this study provide practical information to clarify the syntaxonomy of natural pine forest of the Korean peninsula by the phytosociological methods.

• Journal of the Korean Wood Science and Technology
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• v.39 no.3
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• pp.244-251
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• 2011

The combustion characteristics were evaluated for Japanese Red Pine (Pinus Densiflora) and Korean Pine (Pinus Koraensis). These two species are widely used as building member of Korea-style house and volume density of Japanese Red Pine is relatively higher than that of Korean Pine. The combustion characteristics are closely connected with volume density. The differences of two species in both total heat release (THR) and average heat release rate (HRR) seemed to be resulted from the volume density. Toxicity of smoke from the specimens was increased because of fire-retardant treatment.

• Korean Journal of Environment and Ecology
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• v.21 no.3
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• pp.257-277
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• 2007

We carried out a phytosociological study on pine forests in Korea with the method of Zurich-Montpellier School. We collected the data of 252 $relev{\acute{e}}s$ from 45 sites in the pine forests throughout the Korean Peninsula and its attached islands. The vegetation of the pine forests was classified into one association, three communities and seven subcommunities as follows: A: Quercus mongolica-Pinus densiflora community, A-1: Typical subcommunity, A-2: Vaccinium korean urn subcommunity, A-3 : Rhododendron micranthum subcommunity, B: Quercus serrata-Pinus densiflora community, B-1: Typical subcommunity, B-2: Juniperus rigida subcommunity, B-3: Styrax japonica subcommunity, B-4: Eurya japonica subcommunity, C: Saso-Pinetum densiflorae Yim et al. 1990, and D: Castanopsis cuspidata var. sieboldii-Pinus densiflora community. The former three communities were integrated into the Lindero-Quercion mongolicae Kim 1990 em. 1992. The Castanopsis cuspidata var. sieboldii-Pinus densiflora community remained to be studied in future to determine the association. The communities of Quercus mongolica-Pinus densiflora community was distributed throughout the montane zone in central-northern part of the Korean Peninsula. Quercus serrata-Pinus densiflora community occupied widely in the sub-montane and hilly areas in central and Southern Korean Peninsula. The association of Saso-Pinetum densiflorae was found in Cheju Island. Castanopsis cuspidata var. sieboldii-Pinus densiflora community were distributed in the warm-temperate zone including islands off the south-west coast of the Peninsula.

• Asian Journal of Turfgrass Science
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• v.12 no.4
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• pp.203-210
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• 1998

Environmental concerns arising from synthetic herbicides in plant management systems have led to an interest in plant-derived compounds as natural herbicides. Inhibitory effects of compounds extracted with 50% methanol from corn (Zea mays L.) and pine (Pinus densiflora L.) were evaluated on large crabgrass (Digitaria sanguinalis (L.) Scop.), annual bluegrass Poa annua L.), radish (Raphanus sativus L.), and perennial ryegrass (Lolium perenne L.) The aqueous extracts inhibited seed germination and had postemergence activity on the four species. The stability of biological activity of corn grain, stover, and root extracts was not affected by heating to $135^{\circ}C$ or freezing/thawing treatments when applied at levels above 0.25kg m(sup)-2 based on dry weights of powders before extraction. Heating reduced the activity of pine litter and bark extracts at all levels except the highest application level but had little effect on pine needle extracts.

• Korean Journal of Pharmacognosy
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• v.47 no.3
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• pp.246-250
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• 2016

Pinus densiflora contained diverse phenol compounds like flavonoid, phenylpropanoid and tannin. PineXol$^{(R)}$ is nutraceutical preparation which was treated from bark of Pinus densiflora. Validation and contents determination of taxifolin, (+)-catechin and procyanidion B1 for the preparation of Pinus densiflora (PineXol$^{(R)}$) were confirmed using High-Performance Liquid Chromatography (HPLC). As a result, content of taxifolin, (+)-catechin and procyanidin B1 were, respectively 4.90%, 2.35% and 8.19%. These analysis method and results could be used as important basic data for the preparation of Pinus densiflora.

• Journal of the Korean Wood Science and Technology
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• v.23 no.1
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• pp.49-53
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• 1995

The terpenoids are one of the secondary metabolities in Pinus species and functioned the protection them from insects or/and fungus etc. Since Pinus koraiensis has not any damage by Pine needle gall midge but Pinus densiflora has great injury by them in South Korea. It may great helf to find these reasons to analize their monoterpenenoids and to compare the seasonal variation of their amounts. Terpenoids were analized by GC, GC/MS. Some biogenetic analysis of terpenoids also was elucidated. The seasonal variation of terpenoid was distinguished at March and August and/or September. Most of major component of monoterpenes were increased or decreased in these periods in Pinus koraiensis. Especially 3-carene was showed significant variation between the period of physiological activity and the period of domain. The effect of infection by Pine needle gall midge was significant in Pinus densiflora for amount of monoterpene during the period of infection. The components of p-menthane series between Pinus densiflora and Pinus koraiensis was recognized a significant difference during the experiment. Biogenetic cooccurrance of pmenthane series also was recognized between 3-carene and terpinolene in Pinus koraiensis.

• Current Research on Agriculture and Life Sciences
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• v.19
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• pp.31-37
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• 2001

Uyeki(1928) classified Pinus densiflora into six ecotypes(Northeastem type, Middle-southern flat type, middle-southern upland type, Wibong type, Ankang type, and Geumgang type) based on the pine tree type. The bark color of Geumgang type was ash-brown color on the lower parts of stem and yellowed color on the upper parts of stem. We investigated the physicochemical properties of soil forests to obtain basic data for preservation of exellent pine (Pinus densiflora for. erecta Uyeki). The results were as follows; The soil texture of the Pinus densiflora for. erecta Uyeki forests were showed nearly as sandy loam, that is, sand, silt and clay were consisted of 72%, 15% and 13%, respectively. Soil acidity(pH 4.6) was lower than Korea average forest soil acidity(pH 5.2). The average contents of available phosphate was 11.7ppm at Sokwang-ri, 26.8ppm at Mt. Eungbong, 24.2ppm at Mt. Kumma. It was the lowest at Uljin(4.6ppm). The contents of carbon was 6.2% at Mt. Chungok, 6.1% at Mt. Eungbong. This value was more than average of Korea forest soil.