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Production of Carrot Pomace Fortified with Mucilage, Fibrinolytic Enzyme and Probiotics by Solid-state Fermentation Using the Mixed Culture of Bacillus subtilis and Leuconostoc mesenteroides

  • Jung, Hye-Won;Lee, Sam-Pin
    • Preventive Nutrition and Food Science
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    • v.14 no.4
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    • pp.335-342
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    • 2009
  • Bioactive compounds were produced from carrot pomace by solid-state fermentation using Bacillus subtilis HA and Leuconostoc mesenteroides. The carrot pomace (CP) fermented by B. subtilis HA with 3% monosodium glutamate (MSG) showed higher production of various bioactive compounds, with 1.64 Pa·sn of consistency, 2.31% of mucilage content, 16.95 unit/g of fibrinolytic enzyme activity, 35.3 unit/g of proteolytic activity and 37.5 mg% of tyrosine content. The mucilage production was greatly dependent upon the concentration of MSG added. Most MSG added in CP was converted into mucilage (2.3%) including 0.83% poly-$gamma$-glutamic acid (PGA) with 1,505 kDa of molecular weight. The CP fermented secondly by Leuc. mesenteroides showed acidic pH and lower consistency. However, the fibrinolytic and proteolytic activities were increased. The secondly fermented CP showed the viable cell counts with $2.5{\time}108$ CFU/g of B. subtilis HA and $3.7{\time}109$ CFU/g of Leuc. mesenteroides, respectively. The freeze-dried fermented CP showed 2.88 Pa·sn of consistency, 24% of mucilage content and 104.9 unit/g of fibrinolytic enzyme activity, respectively. Also, the powder of fermented CP indicated viable cell counts of $8.0{\time}107$ CFU/g of B. subtilis and $4.0{\time}108$ CFU/g of Leuc. mesenteroides. Therefore, the fermented CP that was fortified with dietary fibers, fibrinolytic enzyme and probiotics could be utilized as valuable ingredients of functional foods in food or cosmetic industries.

A Parametric Study of Random Amplified Polymorphic DNA (RAPD) Analysis: A Lactobacillus Model (유산균 Lactobacillus 종간의 분류를 위한 RAPD 분석법의 매개변수에 관한 연구)

  • Kwon, Oh-Sik;Yoo, Min;Lee, Sam-Pin
    • Korean Journal of Microbiology
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    • v.34 no.1_2
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    • pp.51-57
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    • 1998
  • A study was carried out to understand some parameters affecting on RAPD analysis with Lactobacillus species. From the results, we found that appearance of specific DNA bands were very influenced by the concentration of $MgCl_2$ but it was overcome by applying enough amount of Taq DNA polymerase. Other parameters such as concentrations of template DNA, random primers and Taq DNA polymerase have enhanced the production of specific DNA bands by increasing their concentration applied. However, we noticed that G/C contents of random primers did not show any correlations with number of specific RAPD bands generated but the RAPD results were heavily influenced by the characteristics of the random primers, that is, the sequences of the oli.

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A Study on a Morphological Identification of Pinelliae Tuber, Typhonii Flagelliformis Rhizoma and Arisaematis Rhizoma (반하(半夏), 수반하(水半夏) 및 천남성(天南星)의 형태(形態) 감별에 관한 연구)

  • Choi, Young-Seong;Kil, Ki-Jung;Lee, Young-Jong
    • The Korea Journal of Herbology
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    • v.22 no.2
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    • pp.103-108
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    • 2007
  • Objectives : A morphological classification among Pinelliae Tuber, Typhonii Flagelliformis Rhizoma and Arisaematis Rhizoma was made through microscopic observation. Method : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Result : 1. Pinelliae Tuber is like a ball with a $1{\sim}1.5cm$ diameter, with a concave apex and pockmarked root trace around. 2. Typhonii Flagelliformis Rhizoma is somewhat oval or like a cone, with a $4{\sim}8mm$ diameter, a $7{\sim}15mm$ height, a projected round apex, and a pin-pointed back. 3. Arisaematis Rhizoma is somewhat flat round with a $2{\sim}2.5cm$ diameter, a little than Pinelliae Tuber, and its apex has incomplete lobopodium and root trace. 4. Fundamental tissues of Pinelliae Tuber, Typhonii Flagelliformis Rhizoma, and Arisaematis Rhizoma have starch grains and mucilage cells, and their mucilage cells include the raphides of calcium oxalate. Conclusion : Internal forms of Pinelliae Tuber, Typhonii Flagelliformis Rhizoma, and Arisaematis Rhizoma are difficult to distinguish from one another, but Pinelliae Tuber and Arisaematis Rhizoma can be distinguished by their external forms and sizes, and Pinelliae Tuber and Typhonii Flagelliformis Rhizoma were distinguished by the forms of apex.

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Model Analysis of Plate using by Digital Test System (디지털 실험장치를 이용한 판의 모우드 해석)

  • Hong, Bong-Ki;Bae, Dong-Myung;Bae, Seong-Yoeng
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.29 no.1
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    • pp.39-55
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    • 1993
  • Modal Analysis is the process of characterizing the dynamic properties of an elastic structure by identifying its modes of vibration. A mode of vibration is a global property of an elastic structure. That is, a mode has a specific natural frequency and damping factor which can be identified from response data at practically any point on a structure, and it has a characteristic mode shape which identifies the mode spatially over the entire structure. Modal testing is able to be performed on structural and mechanical structure in an effort to learn more about their elastic behavior. Once the dynamic properties of a structure are known its behavior can be predicted and therefore controlled or corrected. Resonant frequencies, damping factors and mode shape data can be used directly by a mechanical designer to pin point weak spots in a structure design, or this data can also be used to confirm or synthesize equations of motion for the elastic structure. These differential equations can be used to simulate structural response to know input forces and to examine the effects of pertubations in the distributed mass, stiffness and damping properties of the structure in more detail. In this paper the measurement of transfer functions in digital form, and the application of digital parameter identification techniques to identify modal parameters from the measured transfer function data are discussed. It is first shown that the transfer matrix, which is a complete dynamic model of an elastic plate structure can be written in terms of the structural modes of vibration. This special mathematical form allows one to identify the complete dynamics of the structure from a much reduced set of test data, and is the essence of the modal approach to identifying the dynamics of a structure. Finally, the application of transfer function models and identification techniques for obtaining modal parameters from the transfer function data are discussed. Characteristics on vibration response of elastic plate structure obtained from the dynamic analysis by Finite Element Method are compared with results of modal analysis.

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Characteristic of Vibration in Windturbine System (풍력발전시스템의 진동특성)

  • Kim, Jung-Su;Lee, Hyoung-Woo;Park, No-Gill;Kim, Young-Duk;Kim, Soo-Yum;Lee, Dong-Hwan
    • Journal of Advanced Marine Engineering and Technology
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    • v.35 no.6
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    • pp.786-795
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    • 2011
  • This paper described the characteristic of vibration in wind turbine system including gearbox housing, gear drive, blade, generator. Especially, in planetary gear set, planet gears are supported by flexible pin. So, in planet gear, to consider not only torsional movement but also lateral movement. And include housing movement, because wind turbine system is supported by mount. To find out the characteristic of vibration, take the excitation source and study campbell diagram in operating range. Results of campbell diagram, resonances are occurred at 81.2HZ, 104.7Hz by 2nd tooth passing frequency. And resonance are also occurred at 264.5HZ, 377Hz, 424.6Hz by 3th tooth passing frequency. From the result, take vibration reduced measures.

Modified Design of Anterolateral Thigh Free Flap for Hypopharyngeal Reconstruction (하인두 재건을 위한 전외측 대퇴부 유리 피판의 변형된 도안)

  • Kim, Sung-Chan;Kim, Eun-Key
    • Archives of Reconstructive Microsurgery
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    • v.21 no.1
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    • pp.14-20
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    • 2012
  • Purpose: Defect after ablation of hypopharyngeal cancer often requires reconstruction by free tissue transfer. Since neo-hypopharynx is totally buried, various methods have been suggested for monitoring. We propose a modified design of anterolateral thigh (ALT) free flap for reconstruction of pharyngolaryngectomy defect, which has an exteriorized part for clinical monitoring and allows for primary closure. Materials and Methods: Three consecutive patients with hypopharyngeal cancer were reconstructed with ALT flap with modified design: 1) distal part of flap was elongated into fusiform shape and used as exteriorized monitoring segment with a deepithelized bridge and 2) proximal part was designed as curve so the maximum width of the flap was reduced to less than 10 cm. Results: Patient 1, 2 had uneventful postoperative course with healthy skin color and fresh pin prick bleeding. In patient 3, defect after cancer ablation was shorter than usual and deepithelized bridge was longer. When the general hemodynamic status of the patient was aggravated in postoperative course, the color of monitoring skin was changed. Viability of the whole flap was confirmed by endoscopy. However, leakage developed after 3 weeks and repair was necessary. In all patients the donor sites were closed primarily. Conclusion: By the modified design of ALT flap, clinical monitoring can be possible by examining exteriorized monitoring flap and also donor site can be closed primarily. However possibility of false positive exists and technical caution and patient selection is needed because of danger of leakage.

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Transcriptional Changes of Plant Defense-Related Genes in Response to Clavibacter Infection in Pepper and Tomato

  • Hwang, In Sun;Oh, Eom-Ji;Oh, Chang-Sik
    • The Plant Pathology Journal
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    • v.36 no.5
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    • pp.450-458
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    • 2020
  • Pepper and tomato plants infected with two Clavibacter species, C. capsici and C. michiganensis have shown different patterns of disease development depending on their virulence. Here, we investigated how pepper and tomato plants respond to infection by the high-virulent or low-virulent Clavibacter strains. For this, we chose two strains of each Clavibacter species to show different virulence level in the host plants. Although low-virulent strains showed less disease symptoms, they grew almost the same level as the high-virulent strains in both plants. To further examine the response of host plants to Clavibacter infection, we analyzed the expression patterns of plant defense-related genes in the leaves inoculated with different strains of C. capsici and C. michiganensis. Pepper plants infected with high-virulent C. capsici strain highly induced the expression of CaPR1, CaDEF, CaPR4b, CaPR10, and CaLOX1 at 5 days after inoculation (dai), but their expression was much less in low-virulent Clavibacter infection. Expression of CaSAR8.2 was induced at 2 dai, regardless of virulence level. Expression of GluA, Pin2, and PR2 in tomato plants infected with high-virulent C. michiganensis were much higher at 5 dai, compared with mock or low-virulent strain. Expression of PR1a, Osmotin-like, Chitinase, and Chitinase class 2 was increased, regardless of virulence level. Expression of LoxA gene was not affected by Clavibacter inoculation. These results suggested that Clavibacter infection promotes induction of certain defense-related genes in host plants and that differential expression of those genes by low-virulent Clavibacter infection might be affected by their endophytic lifestyle in plants.

Structural performance by strengthening types of wood frames using H shaped steel joints (H형강 접합부를 갖는 목조 골조의 보강형식에 따른 구조성능)

  • Kim, Soon-Chul;Moon, Youn-Joon;Yang, Il-Seung;Park, Geun-Hong
    • KIEAE Journal
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    • v.8 no.3
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    • pp.77-83
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    • 2008
  • The effective mixture of structural laminated timber and other materials is expected to extend the potentials of building structures because of the potentials to realize high performance in structural safety. The classical joint types using drift pin and bolts are occurred local failures due to the small bearing area. In result, new joints using H shaped steel were suggested in this research. The objective of this study is to evaluate elasto-plastic behaviors by strengthening types of wood frames with new joints connecting structural laminated timber with H shaped steel. A total of five specimens of about one-second scale were tested. Specimens had columns with 1,050 height and $84mm{\times}100mm$ section, and a beams with 1,950mm length and $130mm{\times}100mm$ section. Also, the specimens were stiffened by brace, hwang-toh brick, and autoclaved lightweight concrete. The results of the test showed that the specimen stiffened with autoclaved lightweight concrete was characterized by fairly good strength and stiffness than those of the other specimens. Initial stiffness of H-2.0D-NS specimen with 2 times inserting length of beam height showed 1.33 times than that of H-1.5D-NS specimen. However, the strength of H-2.0D-NS specimen has not improved too much than H-1.5D-NS specimen.

Stafne Bone Cavity of the Mandible

  • Lee, Jae Il;Kang, Seok Joo;Jeon, Seong Pin;Sun, Hook
    • Archives of Craniofacial Surgery
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    • v.17 no.3
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    • pp.162-164
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    • 2016
  • Stafne bone cavity is a rare mandibular defect that was first reported by Edward C. Stafne in 1942. It commonly presents with a well-demarcated, asymptomatic, unilateral radiolucency that indicates lingual invagination of the cortical bone. A 52-year-old female patient who with nasal bone fracture, visited the hospital. During facial bone computed tomography (CT) for facial area evaluation, a well-shaped cystic lesion was accidentally detected on the right side of the mandible. Compared to the left side, no swelling or deformity was observed in the right side of the oral lesion, and no signs of deformity caused by mucosal inflammation. 3D CT scans, and mandible series x-rays were performed, which showed a well-ossified radiolucent oval lesion. Axial CT image revealed a cortical defect containing soft tissue lesion, which has similar density as the submandibular gland on the lingual surface of the mandible. The fact that Stafne cavity is completely surrounded by the bone is the evidence to support the hypothesis that embryonic salivary gland is entrapped by the bone. In most cases, Stafne bone cavity does not require surgical treatment. We believe that the mechanical pressure from the salivary gland could have caused the defect.

A Study on the Toothbrush-Dentifrice Abrasion of Class V Restroations (치경부 5급 와동 수복의 잇솔질 마모에 관한 연구)

  • Hwang, Su-Jin;Yu, Mi-Kyung;Lee, Kwang-Won
    • Journal of Dental Rehabilitation and Applied Science
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    • v.21 no.1
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    • pp.69-81
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    • 2005
  • The objective of this study was to evaluate the toothbrush abrasion characteristics of class V restorations. Thirty extracted human premolars, which were collected from oral surgery clinics were used. We mounted five teeth in a metal ring mold of 50 mm in diameter and 15 mm in height using chemically cured acrylic resin. Class V cavities were prepared in lingual cervical root surfaces and restored using one of following restorative materials : Dentin Conditioner/Fuji II LC (Group FL), All Bond II/Z-250 (Group ZT), One-up Bond F/Palfigue Estelite (Group PE), F2000 Primer/Adhesive (Group FT), and Prime & Bond 2.1/Dyract AP (Group DR). They were stored under distilled water at $37^{\circ}C$ for seven days. The toothbrush abrasion test was conducted using a wear testing machine of pin-on disk type under a load of 1.5 N for 100,000 cycles. We have examined the bonded interfaces, the changes of surface roughness and color of abraded surfaces. From this experiment, the following results were obtained. 1. The change of surface roughness showed high degree: RMGIC>compomer>composite resin (p<0.05). 2. Because of the protrusion and missing of filler particles, SEM observation of abraded surfaces of RMGIC and compomers revealed the increase of surface roughness due to the selective removal of matrix resin. 3. The color change by toothbrush abrasion was affected in large part by the change of $L^*$ and $b^*$ of resin composites (p<0.05). 4. The color change by toothbrush abrasion was so small to detect by human eyes. 5. SEM observation of abraded surfaces revealed the interface bonding was the best in the FT group.