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Inhibition of Adipocyte Differentiation and Adipogenesis by Supercritical Fluid Extracts and Marc from Cinnamomum verum (초임계 추출 계피오일의 3T3-L1 지방전구세포의 분화 전사인자 억제에 의한 지방대사 조절)

  • Park, Sung-Jin;Lee, In-Seon;Lee, Sam-Pin;Yu, Mi-Hee
    • Journal of Life Science
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    • v.23 no.4
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    • pp.510-517
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    • 2013
  • This study was performed to evaluate the antiobesity effect of supercritical fluid extracts (SFC) and marc methanol extracts (SFM) from Cinnamomum verum in 3T3-L1 preadipocytes. In inducing the differentiation of 3T3-L1 preadipocytes in the presence of an adipogenic cocktail, iso-butylmethylanthine (IBMX), dexamathasone, and insulin, treatment with fraction residue SFC and SFM. SFC significantly reduced the mRNA expression of the transcription factor peroxisome proliferator-activate-dreceptor-${\gamma}$ ($PPAR{\gamma}$), the sterol regulatory-element-binding protein-1c (SREBP1c), and the CCAAT enhancer-binding-protein ${\alpha}$ ($C/EBP{\alpha}$) in a concentration-dependent manner. Moreover, SFC markedly down-regulated acyl-CoA synthetase-1 (ASC1), fatty acid synthesis (FAS), fatty acid transport-1 (FATP1), fatty acid binding protein 4 (FABP4), and perilipin. These findings suggest that SFC may be a potential therapeutic adjunct for obesity by targeting the differentiation of preadipocytes, as well as their functions.

Development of Jelly Fortified with Lactic Acid Fermented Prickly Pear Extract (백년초 젖산 발효액을 이용한 젤리의 제조)

  • Son, Min-Jung;Whang, Ki;Lee, Sam-Pin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.3
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    • pp.408-413
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    • 2005
  • Rheological properties and sensory characteristics of prickly pear jelly made from κ-carrageenan and glucomannan were examined. Prickly pear extract (PPE) fermented by Lactobacillus casei LS (isolated from soy milk curd residue) exhibited pH 3.12, 1.15% acidity (lactic acid) and 2.7 × 10/sup 9/ CFU/mL lactic acid bacteria. In the effect of concentration of fermented PPE, the jelly containing 4% fermented PPE had higher springiness and slightly lower hardness than that without fermented PPE, and was superior to color and taste. The hardness and brittleness of jellies were enhanced by the addition of 0.25% citric acid. The addition of 0.25% ascorbic acid resulted in the production of soft jelly like pudding. In the effect of concentration of κ-carrageenan, the hardness and springiness of jellies were increased by adding 0.75% κ-carrageenan, but was decreased by adding at the 0.9% level. In the overall preference of jellies including sweet and sour taste, higher score was obtained by the fortification of 0.6% and 0.75% κ-carrageenan, 0.25% citric acid and 30% fructose syrup.

Physicochemical and Rheological Evaluation of Rice-Whole Soybean Curds Prepared by Microbial Transglutaminase (미생물 Transglutaminase를 이용하여 제조된 쌀 혼합 전두부의 이화학적 및 물성 평가)

  • Jin, Ik-Hun;Lee, Sam-Pin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.5
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    • pp.738-746
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    • 2011
  • We manufactured rice-whole soybean curd by a microbial transglutaminase (MTGase) with a mixture of hydrolyzed rice and micronized whole soybean powder (MWSP) and analyzed its rheological properties, including texture, viscoelasticity, protein cross-linking, and surface structure. A 40% rice suspension digested with a Termamyl enzyme at $85^{\circ}C$ for 20 min showed a 9.0% reducing sugar and a consistency of $1.27\;Pa{\cdot}s^n$, resulting in a great reduction in consistency. A MWSP suspension with 22% solid content was transformed into a typical tofu texture. MWSP curd fortified with 7.5% rice showed enhanced texture properties, with a hardness of 639.6 dyne/$cm^2$, and a springiness of 0.96. In a MWSP suspension (18~22% w/v) treated with 5% MTGase, viscoelasticity increased dependently with MWSP concentration, and a 22% MWSP indicated a G' value of 5.1 Pa and a G'' value of 9.0 Pa. Furthermore, soybean proteins present in the 22% MWSP curd largely disappeared or formed polymers with a high molecular weight by MTGase reaction within 30 min. MWSP (22%) fortified with 7.5% rice showed similar polymerization patterns on SDS-PAGE. The surface structure of the rice-MWSP curds was more dense and homogeneous network due to the addition of hydrolyzed rice. However, the surface structure of all rice-MWSP curds became rough and showed a non-homogeneous network after cold storage.

Physicochemical Properties of Carrot Juice Fermented by Leuconostoc mesenteroides SM (김치젖산균에 의한 당근 발효음료의 물리화학적 성질)

  • Jo, Sun-Ja;Oh, Soo-Myung;Jang, Eun-Kyung;Hwang, Ki;Lee, Sam-Pin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.2
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    • pp.210-216
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    • 2008
  • Leuconostoc mesenteroides SM isolated from a raw carrot was used for the fermentation of carrot juice (CJ). Lactic acid fermentation of CJ was performed at $25^{\circ}C$ for 24 hr with $0{\sim}20%$ sucrose concentration, resulting in the production of mucilage. The fermented CJ showed the complete conversion of sugar into dextran after fermentation for 24 hr in the presence of sucrose below 15% concentration. With 15% sucrose, the fermented CJ had pH 3.8, 0.9% acidity, consistency index of $3.5\;Pa{\cdot}s^n$. After cold storage for 3 weeks, fermented CJ showed slight increase in acidity and relatively constant value in pH; however, the consistency index, red color value and viable cell counts were slightly decreased for 3 weeks. The ${\beta}-carotene$ content in CJ was drastically decreased by lactic acid fermentation for 24 hr, indicating $72\;{\mu}g/mL$ as compared to $142\;{\mu}g/mL$. The fermented CJ had improved body texture, red color and stability without sedimentation.

A new natural habitat of Abeliophyllum distichum Nakai (미선나무(Abeliophyllum distichum Nakai)의 새로운 자생지 보고)

  • Kim, Dong-Kap;Kim, Joo-Hwan
    • Korean Journal of Plant Taxonomy
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    • v.38 no.4
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    • pp.573-582
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    • 2008
  • We report a newly found natural habitat of Abeliophyllum distichum in mountainous slope range of Yeongdong-gun, Chungbuk Province. Abeliophyllum distichum Nakai is one of the Korean monotypic endemic species. Natural growth habitats of this species have been recorded from seven sites up to now, and all of the natural habitats are located in middle (Chungbuk Prov.) and middle west (Jeonbuk Prov.) parts of South Korea. Among the previously recorded seven natural habitats, six sites have been designated as Korean national monuments and protected with in situ conservation. New natural habitat of A. distichum is located on northwest slope of stiff hillock area beside the small stream, Seolgye-ri, Yeongdong-eup, Yeongdong-gun, Chungbuk Province. Total growing area is nearly $3,000 m^2$. It is 10-25 cm in soil depth and pH 5.0-6.5 in soil acidity in that area. And many of A. distichum are clustered with 2-5 individuals extended by stoloniferous asexual reproduction. And the total numbers of A. distichum are about 700 individuals with only typical white flowers, and the ratio between pin type and thrum type is 37% and 63%, respectively. The huge population of A. distichum is growing with Quercus mongolica-Fraxinus rhynchophylla association in a mixed forest, and it shows high affinity with Stephanandra incisa, Ligustrum obtusifolium, Euonymus alatus for. ciliatodentatus, and Smilax sieboldi.

Antimicrobial Effect of the Submerged Culture of Sparassis crispa in Soybean Curd Whey (순물을 이용한 꽃송이 버섯 균사체 배양액의 항균활성 평가)

  • Lee, Eun Ji;Kim, Ji-Eun;Park, Min-Ju;Park, Dong-Cheol;Lee, Sam-Pin
    • Food Science and Preservation
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    • v.20 no.1
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    • pp.111-120
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    • 2013
  • Sprassis crispa was cultivated using soybean curd whey, and its antimicrobial activities were examined against those of eight microorganisms that were foodborne pathogens or food-poisoning bacteria. The culture broth of soybean curd whey was superior in mycelium content (17.76 g/L) to that of the defined culture broth, and the ${\beta}$-glucan content was about 10.64 percent (w/w). The antimicrobial activities of the culture broth were confirmed against those of B. cereus, St. aureus, L. monocytogenes and S. typhimurium using the paper disk method. The antimicrobial activity was also maintained after the heat treatment and alcalase treatment. The filtrate with less than 3 kDa M.W. also showed the antimicrobial activity against four strains: B. cereus, St. aureus, L. monocytogenes and S. typhimurium. The minimum inhibitory concentration (MIC) was about 1.26 mg/mL in the B. cereus and 12.6 mg/mL in the St. aureus and L. monocytogenes. The S. typhimurium showed a MIC of 62.8 mg/mL. Thus, the culture of Sparassis crispa using soybean curd whey provides a thermally stable antimicrobial agent that can be used as a natural preservative in the biofood industry.

Evaluation of the Chemical Compositions of Solar Salts Produced in Korea (국내 시판 천일염의 성분 조사)

  • Seo, Ji-Hyun;Kim, Hyun-Jeong;Lee, Sam-Pin
    • Food Science and Preservation
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    • v.19 no.4
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    • pp.554-559
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    • 2012
  • To evaluate the effect of aging on solar salts produced in Korea, the chemical compositions of the solar salts, including their heavy metal contents, were analyzed after they were aged for one-year (OA) and for three years (TA). The average contents of the components were: sodium chloride; 81.4% (OA) and 90.9% (TA), chloride; 57.7% (OA) and 65.0% (TA), moisture content; 4.7% (TA) and 12.0% (OA), insoluble solids; 0.03% (OA) and 0.04% (TA), and $SO_4$ ions; and 0.57% (TA) and 0.85% (OA). No significant difference was found, however, in the heavy metal contents. Heavy metals such as As, Pb, and Hg were detected with values far below the maximum permissible limit, and Cd was not detected in the salts. The TA solar salts showed higher values of sodium chloride, chloride, and insoluble solids than the OA solar salts, but the TA solar salts had lower moisture and $SO_4$ values. These results clearly indicate that the levels of heavy metals in the OA and TA solar salts that are produced in Korea are safe.

Comparison of Nutritional Composition of Noodle Products in Korean Key Foods (국내 주요 식품(Key foods) 중 면류 제품의 영양성분 함량 비교)

  • Cha, Seung-Hyeon;Han, In-Beom;Park, Woo-Hyun;Park, Sang-Beom;Bak, Se-Lim;Kim, Byung Hee;Yoon, Sung-Won;Kim, In Hwan;Chun, Jiyeon;Shin, Jung-Ah;Kim, Younghwa;Shin, Eui-Cheol;Seo, Dongwon;Lee, Sam-pin;Sung, Jeehye;Kim, So-Jung;Lee, Jun-Soo;Jang, Keum-Il
    • The Korean Journal of Food And Nutrition
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    • v.34 no.5
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    • pp.449-457
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    • 2021
  • This study analyzed the nutritional composition (proximate composition, total dietary fiber, calories, minerals, fatty acids, and amino acids) of 10 noodle products (tteok ramyun, jjamppong ramyun, kimchi ramyun, instant udon, cup ramyun, jajangmyun, bibimmyun, cream spaghetti, ssalguksu, and milmyun), which account for 85% of the cumulative intake of one or more key nutrients, using data from the 7th Korea National Health and Nutrition Examination Survey. The moisture contents of bibimmyun, jajangmyun, and cream spaghetti were lower than those of the other noodle products, whereas the crude fat, crude protein, carbohydrate, and calorie contents were the highest. Cream spaghetti had the highest mineral, fatty acid, and amino acid contents, followed by bibimmyun and jajangmyun. Ssalguksu had the lowest contents of most nutrients. These data could be used to populate a food composition database, which can provide consumers with the nutritional information about frequently consumed noodle products.

Numerical simulation of three-dimensional flow and heat transfer characteristics of liquid lead-bismuth

  • He, Shaopeng;Wang, Mingjun;Zhang, Jing;Tian, Wenxi;Qiu, Suizheng;Su, G.H.
    • Nuclear Engineering and Technology
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    • v.53 no.6
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    • pp.1834-1845
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    • 2021
  • Liquid lead-bismuth cooled fast reactor is one of the most promising reactor types among the fourth-generation nuclear energy systems. The flow and heat transfer characteristics of lead-bismuth eutectic (LBE) are completely different from ordinary fluids due to its special thermal properties, causing that the traditional Reynolds analogy is no longer recommended and appropriate. More accurate turbulence flow and heat transfer model for the liquid metal lead-bismuth should be developed and applied in CFD simulation. In this paper, a specific CFD solver for simulating the flow and heat transfer of liquid lead-bismuth based on the k - 𝜀 - k𝜃 - 𝜀𝜃 model was developed based on the open source platform OpenFOAM. Then the advantage of proposed model was demonstrated and validated against a set of experimental data. Finally, the simulation of LBE turbulent flow and heat transfer in a 7-pin wire-wrapped rod bundle with the k - 𝜀 - k𝜃 - 𝜀𝜃 model was carried out. The influence of wire on the flow and heat transfer characteristics and the three-dimensional distribution of key thermal hydraulic parameters such as temperature, cross-flow velocity and Nusselt number were studied and presented. Compared with the traditional SED model with a constant Prt = 1.5 or 2.0, the k - 𝜀 - k𝜃 - 𝜀𝜃 model is more accurate on predicting the turbulence flow and heat transfer of liquid lead-bismuth. The average relative error of the k - 𝜀 - k𝜃 - 𝜀𝜃 model is reduced by 11.1% at most under the simulation conditions in this paper. This work is meaningful for the thermal hydraulic analysis and structure design of fuel assembly in the liquid lead-bismuth cooled fast reactor.

Investigation of the Distance from One Tooth Center to Adjacent Tooth Center and Dental Arch Size to Develope New Removable Die System for Fabrication of Dental Prosthetic Appliance (치과보철물 제조용 모형 조립체(가철식 치형 시스템) 개발을 위한 인접 치아 중심 간격 및 악궁 크기 조사)

  • Kim, Bu-Sob;Ma, Seung-Ho;Jeong, Kyung-Mok;Byun, Tae-Hee
    • Journal of Technologic Dentistry
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    • v.29 no.2
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    • pp.151-161
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    • 2007
  • To obtain the information of dental arch size and the distance from one tooth center to adjacent tooth center of occlusal surface of each tooth which is perforated by Pindex system on working cast for removable die system, 600 dental casts in Busan were examined. The distance of center of occlusal surface of each tooth and dental arch size were digitized. The results were as follows; 1. Mean values of the distance from center of maxillary central incisor to maxillary lateral incisor(tooth number 11$\sim$12, 21$\sim$22) is 5,7 mm, 12$\sim$13(22$\sim$23) is 5.9 mm, 13$\sim$14(23$\sim$24) is 6.9 mm, 14$\sim$15(24$\sim$25) is 7.1 mm, 15$\sim$16(25$\sim$26) is 8.4 mm, 16$\sim$17(26$\sim$27) is 10.2 mm, 11$\sim$21 is 7.30 mm. Mean values of the distance from center of mandibular central incisor to mandibular lateral incisor(tooth number 31$\sim$32, 41$\sim$42) is 4.5 mm, 32$\sim$33(42$\sim$43) is 4.8 mm, 33$\sim$34(43$\sim$44) is 6.3 mm, 34$\sim$35(44$\sim$45) is 7.2 mm, 35$\sim$36(45$\sim$46) is 9.2 mm, 36$\sim$37(46$\sim$47) is 10.7 mm, 31$\sim$41 is 4.7 mm. 2. Mean values of the distance from the center of maxillary right central incisor to the center of maxillary left central incisor(11$\sim$21) is 7.3 mm, 12$\sim$22 is 18.2 mm, 13$\sim$23 is 26.9 mm, 14$\sim$24 is 37.2 mm, 15$\sim$25 is 43.2 mm, 16$\sim$26 is 48.5 mm, 17$\sim$27 is 53.5 mm. Mean values of the distance from the center of mandibular right central incisor to the center of mandibular left central incisor(31$\sim$41) is 4.7 mm, 32$\sim$42 is 13.3 mm, 33$\sim$43 is 21.7 mm, 34$\sim$44 is 31.9 mm, 35$\sim$45 is 38.2 mm, 36$\sim$46 is 44.8 mm, 37$\sim$47 is 50.7 mm. 3. Mean values of the distance from the line of between 11$\sim$21 to 12$\sim$22 is 10.9 mm, 12$\sim$22 to 13$\sim$23 is 8.7 mm, 13$\sim$23 to 14$\sim$24 is 10.3 mm, 14$\sim$24 to 15$\sim$25 is 6.0 mm, 15$\sim$25 to 16$\sim$26 is 5.3 mm, 16$\sim$26 to 17$\sim$27 is 5.0 mm. 31$\sim$41 to 32$\sim$42 is 8.6 mm, 32$\sim$42 to 33$\sim$43 is 8.4 mm, 33$\sim$43 to 34$\sim$44 is 10.2 mm, 34$\sim$44 to 35$\sim$45 is 6.3 mm, 35$\sim$45 to 36$\sim$46 is 6.6 mm, 36$\sim$46 to 37$\sim$47 is 5.9 mm. 4. We checked the bottom side of cast to verify the position of dowel pin. There is no difference upper side and lower side.

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