• Korean Journal of Food Science and Technology
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• v.21 no.1
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• pp.58-62
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• 1989

Free amino acids in water-extracts and total amino acids in hydrolysates of three cultivated mushrooms, Agaricus bisporus, Pleurotus ostreatus and Lentinus edodes were analyzed by amino acid analyzer to know the compositional differences depending on species and portions (pileus and stipe). Eighteen amino acids were identified and quantified. The total nitrogen and protein nitrogen contents were in the range of 1.67-6.24% and 0.88-2.42% (dry basis), respectively. The considerable differences were often found among species and portions of mushrooms. All of them were found to be higher in the pileus part. The free amino acids conents in water-extracts were in the range of 10.04-37.85mg/g(dry weight) and the total amino acids contents in hydrolysates were in the range of 53.37-120.15mg/g(dry weight). Glutamic acid, serine, histidine, and alanine were dominent in the free amino acids pool and glutamic, aspartic acid, histidine, and alanine were in the total amino acids pool.

• Journal of Mushroom
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• v.18 no.2
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• pp.174-177
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• 2020

This study sought to determine the optimal mixing ratio of Korean cultivated Artemisia annua for production of functional oyster mushrooms. After 23 days of cultivation, mycelial growth was 12.7 cm in medium supplemented with 5% A. annua and 12.5 cm in control. Mycelial growth progressively slowed with the addition of A. annua, with barely any growth (2.1 cm) in the presence of 70% A. annua. Mycelial density was high density without significant difference between treatments. The pileus diameter was greater in the presence of A. annua than in the control, but the pileus thickness was only slightly higher compared to the control. The stipes thickness was greatest for 15% A. annua, and the length of stipes was longest at 10% A. annua, but was lower than the value of the control. The L value of the fruiting zone was highest 10% firewood, and the L value of freshwater was highest at 5% A. annua. The fruiting body yield was highest to (122 g/850 ml) in medium supplemented with 5% A. annua but markedly decreased at higher levels of A. annua.

• Journal of Practical Agriculture & Fisheries Research
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• v.22 no.1
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• pp.99-112
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• 2020

A edible mushroom, Hypsizygus marmoreus is commercially cultivated. However, the researches of cultivation and physiological characteristics were not conducted in Korea. In this study, we conducted on artificial cultivation of H. marmoreus and elucidated the effect of light on the mycelial growth and fruit body development using LED light sources with different wavelength; blue (peak wave length 460nm), green(peak wave length 530nm), yellow(peak wave length 590nm), red(peak wave length 630nm), and white as positive control. Mycelial growth of H. marmoreus strains were inhibited about 30~40% in inhibition ratio under the illumination with blue, green, yellow LED light. However, red LED light was not inhibited. Elongation of stipe was effective under the long wave length such as yellow and red light. Abnormal fruit body was produced under the long wavelength and dark. However, development of pileus was effective under the short wavelength such as green and blue light. Also, as a result of cultivation with mixed light for high quality and harvest, many effective numbers and yields of fruiting bodies were obtained in the mixed treatment of blue and white light, and pileus developed well.

• Journal of Mushroom
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• v.21 no.3
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• pp.167-172
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• 2023

A new brown variety of Flammulina velutipes, referred to as 'Asakgold,' was bred by crossing two monokaryons isolated from the "Geumhyang2ho" and "Garlmoe" varieties. The pileus color of the new variety is light brown and its shape is hemispherical. During bottle cultivation, the period necessary for mycelial growth was 40 days, 9 days for primordia formation and 15 days for fruiting body growth. The total cultivation period was 64 days, which was similar to that of the control variety "Yeoreumhyang1ho." The pileus of the new variety was similar and the stipe was thinner and longer than that of the control. The number of valid stipes per bottle was 495, which was 37 % higher than that of the control (362), and the yield was 214 g, 16 % higher than that of the control (185 g). The β-glucan content was 28.69 %, which was observed to be 1.6 times more than that of the control.

• The Korean Journal of Mycology
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• v.51 no.4
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• pp.389-396
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• 2023

In this study, dikaryotic strains of Lentinula edodes were generated by mono-mono hybridization using mating type analysis and their fruiting body characteristics were investigated. Approximately 100 monokaryotic strains were isolated from the basidiospores of Sanbaekhyhang, and homokaryotic strains were isolated from Chungheung 1ho using protoplast isolation and regeneration. Their mating types were evaluated and a total of 60 dikaryotic strains were hybridized. Using these strains, fruiting bodies were produced and their characteristics were examined after cultivation on sawdust media. The results indicated that the rate of hybridization was 100% and that 55 of 60 strains formed fruiting bodies. These showed normal pileus and gill structures; however,10 strains also produced fruiting bodies with abnormal pileus and gill structures. The weight and size of the fruiting bodies differed depending on the strains. Overall, further studies are needed for predicting the characteristics of hybridized strains based on their parental strains.

• Journal of Mushroom
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• v.21 no.4
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• pp.261-265
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• 2023

We aimed to develop outstanding domestic varieties suitable for both columnar and cylindrical-shape substrates, aiming to replace foreign varieties. and bred a high-quality new strain named 'Charmjon', using genetic resources collected from Japan and China. The optimal cultivation temperature for Charmjon's mycelial growth was found to be 25℃, and its mycelial growth at 15℃ and 25℃ was superior to the control variety. In terms of mycelial growth characteristics based on the substrate, Charmjon exhibited excellent mycelial strength on PDA medium compared to the control variety. Through columnar and cylindrical-shape substrates cultivation, we assessed the quantity and morphological characteristics of the fruiting bodies. The results confirmed that Charmjon can be produced stably using both cultivation methods, and it showed higher yields and individual weights than the control variety. In addition, the color of the pileus was notably darker, and the shape of the pileus varied depending on the cultivation method. The test of genetic diversity revealed that Charmjon has distinct genetic characteristics compared to the control varieties.

• Mycobiology
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• v.29 no.2
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• pp.80-84
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• 2001

Nine species of genus Ganoderma different in cultural characteristics each other were separated by histo-anatomical differences. Differences among the species and geographic distribution of G. lucidum were also analyzed to provide the criteria for the classification of Korean cultivation or wild type strains. Korean cultivation and wild type strains were quiet different from other species as well as Taiwan and North American G. lucidum strains in histo-anatomical traits of Ganoderma. Pore color of Korean G. lucidum strains was less brown than those of Taiwan and North American strains. Shapes of pores were round or circular in Korean strains but ellipsoidal or angular in Taiwan or North American strains. Pore numbers of Korean strains were more than those($4{\sim}6/mm$) of Taiwan or North American strains. Hardness of the pileus of Korean strains was much more than that of Taiwan or North American strains. Such characteristics of Korean strains were different from those of other species of Ganoderma. Korean G. lucidum strains could be classified into the other group because they had many different traits in growth characteristics of fruiting bodies and histo-anatomical characteristics from those of Taiwan or North American G. lucidum and other species of G. lucidum complex.

• Journal of Mushroom
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• v.2 no.4
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• pp.222-224
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• 2004

Sodam oyster mushroom(Pleurotus ostreatus were bred in Mushroom Research Institute, Gyonggi Province A.R.E.S in 2002. Sodam oyster mushroom were bred and cultivated one after mating single spores collected from Aenutari 1-ho and Suhan 1-ho. The major characteristics of the mushroom are showing a lot of pinheadings, the gray-colored and shallow cornhead-shaped pileus. Around 20 days at $20^{\circ}C$ were required for incubation of Sodam and the yield was shown high by 148.2g/bottle. As the activity of hypha is so strong, the proper time of pinheading is demanded.

• Journal of Mushroom
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• v.10 no.3
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• pp.101-108
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• 2012

Pleurotus ostreatus 'Miso' is a mutant strain showing white color in pileus from the known parent strain 'Wonhyeong 1'. Shape and several other characters also vary with culture conditions. Mating experiments were performed to understand interstrain mating relationship using monokaryons of the parent and the mutant strains. All monokaryons were grown from single spores isolated from freshly collected fruit bodies. Pairings were performed in 90 mm petri dishes on PDA. They were allowed to grow at 25 until two fronts of the advancing mycelia met and developed a conspicuous contact zone. The contact zone and the outer edges of paired colonies on each plate were examined for clamp connections. The parent and the mutant resulted in tetrapolar incompatibility in intrastrain crosses. In interstrain crosses, each monokaryotic tester strain of the parent strain was out-crossed to monokaryotic tester strains of the mutant. As a result of these crosses it was found that both strains share the same A and B incompatibility factors yielding 25% compatibility.

• The Korean Journal of Mycology
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• v.41 no.1
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• pp.28-32
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• 2013

New Shiitake (Lentinula edodes) strain "Chunbaegko" was bred by Di-mon method. Bed-log cultivation of "Chunbaegko" was performed. Fruit-body production of "Chunbaegko" was most at spring and autumn. Optimal temperature of fruit-body formation was $14{\sim}22^{\circ}C$ Baegwhako, the best quality fruit-body, is produced during spring. The diameter of pileus is ca. 52 mm. The total amount of fruit-body production during 4 years(one generation) was 140 $kg/m^3$ log.