• Proceedings of the Korean Society of Crop Science Conference
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• 2007.11a
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• pp.253.1-253.1
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• 2007

• Korean Journal of Pharmacognosy
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• v.45 no.3
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• pp.214-219
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• 2014

Artemisia apiacea is a traditional herbal medicine using treatment of eczema and jaundice in Eastern Asia including China, Korea, and Japan. In this study, the three phytosterol constituents were isolated and identified from the hexane fraction of 80% aqueous methanol extract of A. apiacea. Compounds were isolated using open column chromatography (silica gel). Their chemical structures were also established using $^1H$-NMR and $^{13}C$-NMR. Moreover, neuroprotective activity of each compound against glutamate-induced neurotoxicity in hippocampal HT-22 cell line was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Furthermore, Inhibition of reactive oxygen species (ROS) and calcium ion ($Ca^{2+}$) accumulation were measured for elucidation of neuroprotective mechanism of isolated compounds. They showed that stigmasterol had neuroprotective activity against the glutamate-induced toxicity by inhibition of ROS and $Ca^{2+}$ production. In conclusion, isolated compound of A. apiacea might be useful for therapeutic agent against neurodegenerative diseases.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.spc
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• pp.65-71
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• 2008

The effect of rice bran (RB) applied alone or in combination with chemical fertilizer at different application rate on the phytochemical and antioxidant properties of rice was investigated. The treatments were 3 levels of RB namely: 200% RB (500 kg $10a^{-1}$), 100% RB (250 kg $10a^{-1}$), 50% RB (125 kg $10a^{-1}$), Recommended fertilizer dose (RF: $N-{P_2}{O_5}{K_2}O$, 11-5.5-4.8 kg $10a^{-1}$) combined with each RB, Half-recommended fertilizer dose (HRF: $N-{P_2}{O_5}{K_2}O$, 5.5-2.75-2.4 kg $10a^{-1}$) combined with each RB, RF and HRF applied at 1, 5, 10 days before rice transplanting (DBT). The parameters investigated were antioxidant, phytosterol and fatty acid contents. Results showed that the antioxidant property and phytosterol contents were high at 10 DBT HRF plus RB, 5 DBT RF plus RB, and 1 DBT 100 and 200% RB. However, total polyphenols increased from 10 to 1 DBT. In the case of fatty acids, no general trend was observed between treatments at different application times. Linoleic acid was high at 10 DBT HRF plus RB while linolenic acid was not affected at different application times. Palmitoeic and oleic acids were not also affected at 5 and 10 DBT. Saturated fatty acids were not also affected by any treatment at different application times except for palmitic acid.Most parameters obtained higher values at 100 and 200% RB treatments in 1 DBT.

• Food Science and Preservation
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• v.17 no.3
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• pp.376-383
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• 2010

Including crude fat content, triacylglycerol species, tocopherols and phytosterols were analyzed in 8 kinds of nuts (sunflower seed, cashew nut, walnut, pistachio, pumpkin seed, ginkgo, hazel nut and pecan). The extracted crude fats showed 0.63~39.60 wt%, among which hazel nut showed the highest amount of fat content. Oleic acid (C18:1) was major fatty acids at sn-2 position in cashew nut, pistachio, hazel nut, and pecan while sunflower seed, walnut, and pumpkin seed showed linoleic acid (C18:2) as a major fatty acids at sn-2 position. Especially, ginkgo contained 10.72 wt% of vaccenic acid (C18:1-n7) at sn-2 position. The TAG species of 8 kinds of nuts were analyzed by reverse-phase HPLC, from which PN value ranged 40~52. Among the analyzed nuts, higher content of tocopherols were observed in ginkgo (48.57 mg/100 g), sunflower seed (38.35 mg/100 g), and pumpkin seed(31.43 mg/100 g). Total phytosterols were observed with the range of 88.60~947.20 mg/100 g.

• Journal of Nutrition and Health
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• v.53 no.2
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• pp.99-110
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• 2020

Purpose: The rice germ fraction is a better source of protein, lipid, and fiber than the rice endosperm. Furthermore, the rice germ is rich in bioactive phytochemicals such as γ-aminobutyric acid, tocopherols, tocotrienols, phytic acid, and so on. In this study, the phytosterol content and antioxidant activity of Keunnunjami germ (KG) or normal rice germ supplement were investigated in healthy adult rats. Methods: In vitro, quantitative assessment of phytosterols, including β-sitosterol, campesterol, cycloartenol, and stigmasterol, was performed. Comparative antioxidant activities of 2 rice germs were measured based on DPPH radical scavenging activity, reducing power, and ABTS radical scavenging capacity. In vivo, male Spraque-Dawley rats (30-weeks-old) were randomly assigned a diet of normal control (NC, AIN-93M diet), AIN-93M diet supplemented with normal rice germ 3% (NG3), or AIN-93M diet supplemented with KG 3% (KG3) and fed for 8 weeks. Results: KG contained significantly higher campesterol and stigmasterol contents and antioxidant activity than normal rice germ. The KG3 group exhibited significantly lower body weight gain as well as inguinal and total white adipose tissue weights. There were no significant differences in plasma glucose, insulin, C-peptide, or homeostasis model assessment of insulin resistance level among the 3 groups. The plasma tumor necrosis factor-α concentration was significantly lower while leptin, advanced oxidation protein products, and interleukin-6 showed downward trends in the KG3 group. In addition, the superoxide dismutase level of the KG3 group was significantly higher compared to the NC and NG3 groups. Conclusion: This study indicates that KG can be considered as a valuable source of phytosterol components. Lastly, KG has strong antioxidant properties and may have potential to ameliorate elevation of proinflammatory cytokine production with age.

• The Korean Journal of Food And Nutrition
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• v.26 no.3
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• pp.591-600
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• 2013

This study investigated the changes in the nutraceutical lipid components of brown rice oil after germination. Four different high-yielding cultivars (Dasan1, Segyejinmi, Hanareum1 and Hanareum2) of Korean brown rice were selected and brown rice oil was extracted from each cultivar before and after germination. Free fatty acid, squalene, policosanols, and isomers of phytosterol were analyzed using GC, and isomers of tocols (tocopherol and tocotrienol) and ${\gamma}$-oryzanol were quantified using HPLC from both brown rice oil (BRO) and germinated brown rice oil (GBRO). The contents of phytosterol isomers, campesterol, stigmasterol, and ${\beta}$-sitosterol were increased by 8.3%, 31.6%, 3.3% in GBRO, respectively. Furthermore, the squalene content showed the highest increase of up to 2.4 fold in GBRO compared to BRO. In addition, linoleic and linolenic acid composition increased whereas oleic and palmitic acid decreased in the GRBO. However, the contents of tocols (tocopherol and tocotrienol) in GBRO were lower than those in BRO, and there was no significant difference in policosanol and ${\gamma}$-oryzanol between GBRO and BRO. These results suggest that GBRO has the potential as a healthy and functional source due to its lipid profile on improved lipid metabolism.

• 한국약용작물학회:학술대회논문집
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• 2008.11a
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• pp.241-242
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• 2008

• Proceedings of the Korean Society of Crop Science Conference
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• 2018.04a
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• pp.185-185
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• 2018

• Proceedings of the Korean Society of Crop Science Conference
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• 2018.10a
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• pp.245-245
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• 2018

• Korean Journal of Medicinal Crop Science
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• v.16 no.5
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• pp.337-340
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• 2008

This study was conducted to compare the contents of fatty acids and phytosterols in Glehnia littoralis according to habitat areas. In leaves of G. littoralis collected from Yeong-deok, fatty acids contents was higher than that of the other aeras, while there was no difference in contents of roots and seeds according to habitat areas. Average phytosterols contents of 10 habitat areas were $3.8{\mu}g\;g^{-1}$ (campestreol) $16.8{\mu}g\;g^{-1}$ (stigmasterol) and $3.7{\mu}g\;g^{-1}$ ($\beta$-sitosterol), respectively. G. littoralis collected from Uljin showed the highest phytosterols contents than that of the other aeras.