• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.6
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• pp.652-665
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• 1997

Allelopathic effects on some crops(rice, barley, wheat, Chinese cabbage, leaf mustard, onion, welsh onion, tobacco, red pepper) for extracts of Phytolacca americana and Armoracia rusticana were investigated in MS solid medium. Germination percentage, radicle length, hypocotyl length, plant height, fresh weight, dry weight, and seedling vigor were rapidly inhibited by methanol extract from Phytolacca americana according to increase of the concentration in test plants. Autotoxicity of Phytolacca americana began to appear at concentrations greater than 50$\mu$l methanol extracts per 20ml medium. The fresh weight and dry weight of Phytolacca americana were also decreased by its own methanol extract according to increase of the concentration. Initial amounts of growth of all crops were also inhibited by methanol extracts from Armoracia rusticana, but the inhibitory effects were lowered than those of methanol extracts of Phytolacca americana. Of four fractions, ethyl acetate, petroleum ether, $1^{st}H_2O, 2^{nd}H_2O$, reextracted from methanol extracts of Phytolacca americana, the ethyl acetate fraction showed the highest allelopathic effects on germination percentage and initial amounts of growth. Chlorophyll contents of rice, barley and Chinese cabbage were more inhibited in the ethyl acetate fraction than in the other fractions. Free proline content of Chinese cabbage was increased 31.2 times in the 100$\mu l$ ethyl acetate fraction, and the contents of rice and barley were also increased according to concentration levels in the ethyl acetate fraction.

• Applied Biological Chemistry
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• v.46 no.4
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• pp.385-390
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• 2003

An antimicrobial peptide was purified from the roots of Phytolacca americana L. and was designated as PAMP-r. Purification was carried out by DEAE-cellulose anion exchange, sephadex G-75 gel filtration, Mono S cation exchange, and Resource RPC reverse phage chromatography. The molecular weight of PAMP-r was estimated to be about 4,900 Da by 15% SDS-PAGE under reducing condition. PAMP-r exhibited a broad spectrum of antimicrobial activity. PAMP-r was stable against heat and pH treatment; its activity was not diminished by the heat treatment up to $80^{\circ}C$ for 30 min, and it showed a pH stability in the range between pH 3.0 to pH 8.0.

• Journal of Forest and Environmental Science
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• v.34 no.4
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• pp.321-337
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• 2018

The characteristics of 124 Phytolacca americana present areas and 88 surrounding absent areas over the country were investigated and compared from July 2016 to Sept 2017. The Phytolacca americana was confirmed as having been nationally distributed and is usually found in roadsides at an altitude of 380 m (26%), forest edges (24%), forests (19%), sunny spots (60%), dry soils (53%), sandy loams (31%) and sand+gravel soils (22%). 321 taxons consisting of 79 families, 209 genera, 289 species, 2 subspecies, 26 varieties and 4 cultivars and 286 taxons consisting of 76 families, 195 genera, 256 species, 2 subspecies, 24 varieties and 4 cultivars were found in the Phytolacca americana present areas and the absent areas, respectively. The relative net contribution degree was found highest in the Phytolacca americana (100) followed by Robinia pseudoacacia (29.4), Humulus japonicus (8.8) and Artemisia princeps (6.0) in the present areas, while it was found highest in Robinia pseudoacacia (100) followed by Humulus japonicus (44.4), Artemisia princeps (35.9), Pueraria lobata (18.6) and Setaria viridis (12.3) in the absent areas. The Raunkier's life form in the present/absent areas was found highest in the therophytes (34%/34%), followed by hemicryptophytes (17%/18%), megaphanerophytes and mesophanerophytes (18%/17%), nanophanerophytes (11%/11%) and geophytes (9%/11%). There was no significant difference in the species diversity between the present (2.141) and the absent (2.124) areas. However, the allelopathic agent and light blocked-out by the Phytolacca americana could possibly inhibit germination and the development of other species; especially, where a difference in species diversity was found high in forests, compared to other habitats (p=0.0605). Accordingly, it is suggested to review possible disturbances of native species habitats in shady spots including forest gaps, although the effect of Phytolacca americana on the ecological system in the suburban open and disturbed areas is not large.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.3
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• pp.366-373
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• 2015

This study was conducted to clarify the antimicrobial effect, antioxidant and tyrosinase inhibitory activities of the biological composition having the Phytolacca americana, and to enhance the natural materials utilization of foods and cosmetics. The antimicrobial activities of the different parts of P. americana were evaluated using the agar diffusion test. The antimicrobial activity of P. americana was relatively high in Malassezia furfur known as a skin fungi and Vibrio parahaemolyticus compared to Escherichia coli and Staphy-lococcus epidermidis. However, the antimicrobial activity in Vibrio parahaemolyticus did not show at all parts of P. americana. Both the DPPH radical scavenging activity and ABTS radical scavenging activity have been increased with the higher concentration of methanol extract. In particular, leaf extract of P. americana exhibited the highest activity both ABTS radical scavenging activity and DPPH radical scavenging activity. The nitrite scavenging activity was decreased when the pH was changed from pH 1.2 to pH 6.0. The highest nitrite scavenging activity was exhibited from the methanol extract of fruit, followed by root, stem, and leaf at pH 1.2. However, the nitrite scavenging activity at pH of 6.0 was not almost detected. All plant parts of P. americana showed tyrosinase inhibitory activity. The highest activity was found in the stem, and followed by root, leaf, and fruit in order. These tyrosinase inhibitory activity was progressively increased in a concentration-dependent manner. In this experiment on the methanol extracts of different organ from P. americana, we confirmed that the extract of P. americana showed potent tyrosinase inhibitory activity. Taken together, we conjectured that the P. americana had the potent biological activities, therefore this plant having various functional components could be a good material for development into source of natural food additives and cosmetics.

• YAKHAK HOEJI
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• v.19 no.1
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• pp.9-15
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• 1975

The constitution of genin A-a new triterpenoid isolated from the roots of Phytolacca americana L. has been shown to be 3-oxo-30-carbomethoxy-24-norolean-12-en-28-oic acid. This compound is not a genuine spogenin but an artifact produced during hydrolysis of the Phyolacca saponins.

• The Korean Journal of Ecology
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• v.21 no.1
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• pp.35-45
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• 1998

This study was carried out for the identification and bioassay of bioactive compounds which isolated from Phyutolacca americana L. obtained results are summarized as follows: 1. Two biological active compounds were found from crude extracts of P. americana roots, using the systematic solvent fractionations, such as ethyl acetate fraction and butanol fraction. One biological compound was isolated from the ethyl acetate fraction with silicagel column chromatography, which was identified as a ${\alpha}-spinasterol$ by spectral analysis of IR, H-NMR, C-NMR and MS. The other one is isolated from butanol fraction which was identified as phytolaccoside E by spectral analysis of IR, H-NMR, C-NMR and MS. 2. The ${\alpha}-spinasterol$ and phytolaccoside E induced necrosis of primary root and resulted in death of the tested plant. These two compounds strongly inhibited to the growth of Mucor racemous and Phytophthora infestants but did not inhibited the growth of Colletotricum lagenarium and Fusarium oxisporum. 3. Cytotoxicity of the two biological active compound was exammined to the two different animal cancer cell lines (L1210, K562). The phytolaccoside E has some cytotoxical activity to the growth of cancer cell lines (L1210, K562) but $\alpha$-spinasterol has not cytotoxical effect.

• Proceedings of the Zoological Society Korea Conference
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• 1994.10a
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• pp.196.1-196
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• 1994

• Korean Journal of Agricultural Science
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• v.23 no.2
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• pp.177-181
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• 1996

Biological activities of MeoH extract of Phytolaccn americana L. was investigated. The extract was reextracted with ethyl acetate and fractionated by silica gel colum chromatography. The active compound was analysed by IR. $^1H$-NMR, $^{13}C$-NMR and MS and identified as $\alpha$-spinasterol. $\alpha$-spinasterol induced necrosis of primary root and resulted the death of the examed plant. The compound also inhibited growth of Mucor racemous but it showed weak cytotoxicity to 2 animal cancer cell lines (L1210, K562).

• The Korean Journal of Ecology
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• v.20 no.3
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• pp.181-189
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• 1997

Phenolic compounds from 7 species of naturalized invader species and Korean wild plants were analyzed by high performance liquid chromatography. Eleven phenolic compounds including benzoic acid were identified. The extract of naturalized plants was significantly more inhibitory to seed germination and seedling growth of the both naturalized and Korean wild plants. The content of total phenolic compounds in each extract were 43.5 mg/l in Ailanthus altissima as the maximum amount and 25.5 mg/l in Phytolacca americana as the minimum. Phytotoxic substances of ethanol extracts was investigated for antifungal activity against 23 selected fungus species. The antifungal activity of Phytolacca americana showed the greatest clear zone of 23 mm in Aspergillus awamori and its activity had an effect against 6 fungus species. Ailanthus altissima formed the greatest clear zone of 26 mm in Erwinia carotovora sub. sp. carotovora and had an effect agsinst 2 fungus species.

• Proceedings of the Korean Society of Crop Science Conference
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• 1996.10a
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• pp.88-89
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• 1996