• 한국연초학회지
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• 제23권2호
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• pp.133-140
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• 2001

PAP-I cDNA was synthesized from total RNA of Phytolacca americana leaves by RT-PCR, and then subcloned to recombinant vector pBluescript II SK-. Using PCR with primers designed in our laboratory, we could get the 9 deletion mutant PAP-I cDNA fragments. The first of the fragments was deleted by 66bp from immature N-terminal and then the rest were deleted by 90bp sequentially. Sequentially deletion mutant PAP-I cDNAs were inserted to pAc55M, on down-stream of gall promoter. Recombinant pAc55M was transformed to yeast cells, psy1 and the cells were spreaded on SC_urn-/glucose plate media. Colonies on SC_ura-/glucose plate were streaked on the same position of SC_ura-/glucose and SC_ura-/galactose plate, and we selected colonies growing on both plates, which carry non-cytotoxic deleted mutant PAP-I cDNA. We selected 4 deletion mutant PAP-I cDNAs which have not cytotoxicity.

• The Korean Journal of Ecology
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• 제21권1호
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• pp.1-6
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• 1998

Effects of air pollutants on the chemical properties of soil in Yoch'on inductrial complex were investigated. Soil pH showed severe acidity with average value of 4.3$\pm$0.13. Soil pH decreaed along the increase in altitute of Mt. Jeseok. Soil organic matter and total N was 19.2$\pm$3.6% and 4.7$\pm$0.25mg/g, respectively, which were higher than those of unpolluted area, Kongju. Available P was 41.2$\pm$3.7 $\mu$g/g, which was 4 times greater than that of unpolluted area, Kongju. Average content of total-S was 283.5$\pm$14.1 $\mu$g/g, which was 10 times greater than that of unpolluted area. Content of available Al was o.43$\pm$0.03 mg/g, which was 2 times greater than that of unpolluted area. Available Al increased as the soil pH decrease. Contents of exchangeable K, Ca and Mg were significantly lower than those in unpolluted area, because of severe leaching in acid soil.

• The Korean Journal of Ecology
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• 제15권3호
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• pp.247-255
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• 1992

In order to estimate effects of air pollution for herbs in the plnus thunbergii forests arrounding onsan industrial complex, this study was surveyed. number of species of herb also had a difference over 10 species by distance at the source of air pollution, and species diversity and evennes were also increasing at remote sites in industrial complex as woody plants. At the vicinity of industrial complex, $SDR_4$ of calamagrostis arundinatios arundinacea., miscanthus sinensis and cymbopogon tortilis var. goeringii were very high, and those of lianas as cocculus trilobus, lonicera japonica and paederia scandens, and phytolacca americana were low, but because of being higher than other sites, it was able to inferred that lianas had a tolerance to air pollution. The composition of biological spectra of herbs showed to type of $H-D_1-R_{2-3}-1$ at near site in the source of air pollution and $H-D_1-R_{1-3}-t$ at the other sites, and the propotion of lians forms were relatively high at the vicinity of industril complex.

• 환경영향평가
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• 제24권5호
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• pp.499-515
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• 2015

본 연구는 백로류 집단번식지를 구성하는 식물사회의 특성과 변화를 이해하고 기초 생태정보를 제공하고자 수행되었다. 현장조사는 100개 이상의 둥지가 있는 전국 25개 지역을 대상으로 하였다. 백로류 집단번식지의 식물군락은 8개 유형으로 구분되었으며, 17종의 귀화식물을 포함하여 총 60과 129속 167종이 확인되었다. 진단종으로서 호광성이면서 부영양화 또는 산성화된 입지에 내성을 가지는 미국자리공, 쇠무릎, 닭의장풀 등의 기여도가 높았으며, 일년생식물의 구성비율이 높게 나타났다. 입지적으로는 서식지의 0.5km 이내에 선호하는 취식공간 (논, 하천 등의 습지)이 분포하고, 주로 동향인 산지사면에 위치하였다. 집단서식지의 식물사회는 유형별 입지 환경에 대한 식물사회학적 특성은 나타나지 않았으며, 전반적으로 토양 오염 지역, 수관이 개방된 지역 또는 교란이 빈번한 지역 등과 유사한 식물상을 가진다.

• 사물인터넷융복합논문지
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• 제7권4호
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• pp.35-41
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• 2021

천연염색은 전통적으로 세계 여러 나라에서 사용되어 왔으며, 천연염색이 다양해짐에 따라 염색 패턴의 다양성도 확대되고 있다. 본 연구는 4가지 천연염료에 대한 염료와 매염제의 색상 변화를 보다 정확하게 분석하여 사물인터넷에 정량화된 정보를 제공할 수 있는 수치를 제공함으로써 표준화를 마련하고자 했다. Juglans regia Linn에서 추출한 염료에 구리 아세테이트, 철 II 황산염 및 중크롬산 칼륨을 첨가하면 갈색의 원래 색상이 각각 보라색, 카키색 및 암갈색의 다른 색상으로 변경되었다. Sophora japonica L. 또는 Phellodendron amurense Ruprecht에 첨가된 중크롬산 칼륨을 제외하고는 다른 매염제의 농도는 감소되었지만 염색된 실크의 색상 차이는 매우 컸다. 그러나 정도의 차이는 있지만 초산구리와 황산철은 각각 35%와 15%의 색변화를 유도하였다. 종합해볼때, J. regia Linn, S. japonica L.과 P. amurense Ruprecht에는 copper acetate 15 gram을 Phytolacca americana에는 150 gram의 iron이 정확히 첨가된 것이 가장 높은 색상변화를 유도할 수 있다고 확인되었다. 본 연구의 결과는 다양한 매염제에 의한 정확한 색상 변화가 염료 및 매염제에 의한 보다 정확한 색상 유도를 가능하게 하는 중요한 정보로 활용될 수 있음을 시사하였다.

• 한국환경복원기술학회지
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• 제15권2호
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• pp.85-102
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• 2012

The purpose of this study is to offer the raw data for conserving the biodiversity of urban ecosystem by objective surveying and analysing the vascular plants distributed in major sites of Yangsan-si, Gyeongsangnam-do. The results are as follows. The numbers of vascular plants were summarized as 522 taxa including 110 families, 314 genera, 459 species, 4 subspecies, 46 varieties and 13 forms. The rare plants were 5 taxa including Aristolochia contorta, Drosera rotundifolia, Potentilla discolor, Viola albida and Hydrocharis dubia. The endemic plants were 13 taxa including Salix koriyanagi, Carpinus laxiflora, Stewartia pseudocamellia, Weigela subsessilis and so forth. The specific plants by floristic region were 30 taxa including Caltha palustris, Caryopteris incana, Ilex macropoda, Monochoria korsakowii and so forth. The naturalized plants were 44 taxa including Phytolacca americana, Lepidium virginicum, Solanum americanum, Aster subulatus var. sandwicensis, Panicum dichotomiflorum and so forth. The invasive alien plants were 5 taxa including Sicyos angulatus, Ambrosia artemisiifolia, Ambrosia trifida, Aster pilosus and Paspalum distichum.

• Journal of Applied Biological Chemistry
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• 제43권3호
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• pp.125-130
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• 2000

An antiviral protein (CAP30) with ribosome-inactivating activity was purified from the leaves of Chenopodium album L. through ammonium sulfate precipitation and column chromatography using S-Sepharose, Blue-Sepharose, FPLC Suprose12 HR, and FPLC Mono-S. The molecular wight of CAP30 was estimated to be 30kD. CAP30 was thermostable, maintaing its activity even after incubation at $70^{\circ}C$ for 30 min, and was stable in the pH range of 6 to 9. In a cell-free in vitro translation system using rabbit reticulocyte lysate, protein synthesis was inhibited by the addition of CAP30 with an $IC_{50}$ of 2.26pM. The comparison of N-terminal amino acid sequences of this protein with known ribosome-inactivating proteins (RIPs) revealed that it had some sequence homology with PAP-S and PAP-R from pokeweed (Phytolacca americana)and dodecandrin from P. dodecandra, but had no sequence homology with RIPs from other plants belonging to different orders. The mosaic symptoms on tobacco leaves caused by cucumber mosaic virus infection was completely inhibited by 100 ng/ml of the pure CAP30 protein.

• 한국자원식물학회지
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• 제33권4호
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• pp.311-336
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• 2020

본 연구는 세종시 식물자원의 관리방안을 모색하고 식생관리와 희귀 및 멸종위기식물, 식물생태계 보존대책을 마련하기 위한 기초자료 제공에 있다. 연구결과는 다음과 같다. 세종시 일대에 분포하는 식물상은 104과 315속 462종 3아종 59변종 7품종의 총 531분류군으로 확인되었다. 희귀식물은 총 8과 8속 8종 8분류군으로 취약종(VU) 개박하 등 2분류군, 약관심종(LC) 쥐방울덩굴 등 4분류군, 자료부족종(DD) 큰개고사리 1분류군이 각각 출현하여 전체 식물종 중 1.5% 비율로 나타났다. 주목은 생태적으로 중요하지만 수형과 형태가 곧아 조경 식재용으로 개발하는 것이 필요할 것이다. 특산식물은 총 8과 9속 9종 9분류군으로 확인되었으며, 은사시나무, 오동나무는 산림경관용 군락으로 개발하는 것이 필요하다. 식물구계학적 특정식물은 V등급 자리공 등 2분류군, IV등급은 좀현호색 등 4분류군, III등급은 탱자나무 등 8분류군, II등급은 참개별꽃2분류군, I등급은 쇠고비 등 26분류군이 확인되었다. 귀화식물은 18과 38속 48종 1변종 49분류군으로 확인되었으며, 도시화지수(UI) 15.3%, 귀화율(NI) 8.7%로 분석되었다. 산림휴양과 치유에 필요한 유용식물은 식용이 199분류군으로 가장 높았으며, 목초용 193분류군, 약용 178분류군, 관상용 82분류군, 목재용 25분류군, 섬유용 18분류군, 염료용 9분류군 순으로 나타났다. 본 연구는 세종시 남부일대에 분포하는 식물상을 조사 및 분석하기 위해 수행하였다. 주로 조사된 자료는 생태적 가치에 집중된 경향이 있기 때문에 향후 이용객들이 식물에 대한 중요성을 인지할 수 있도록 보전용, 약용, 식용, 관상용 등 구분하여 보전에 대한 중요성 제고와 실생활과 밀접한 자원 발굴 등 자료 구축이 이루어진다면 세종시 남부일대의 식물상에 대한 자원적 및 생태적 가치가 높아질 것으로 판단된다.

• 한국환경복원기술학회지
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• 제13권4호
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• pp.18-29
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• 2010

The vascular plants in ecological forest site on the Gujaebong (Mt.), Hadong were listed 338 taxa; 85 families, 219 genera, 290 species, 1 subspecies, 40 varieties and 7 forms. Divided into 338 taxa; woody plants were 121 taxa (35.8%) and herbaceous plants were 217 taxa (64.2%). Based on the legal protection species, endangered wild plants II were recorded; Aconitum austro-koreense, Corylopsis gotoana var. coreana and rare plants (IUCN) by the Korea Forest Service and Korea National Arboretum 5 taxa were recorded; Botrychium virginianum, Aconitum austro-koreense, Coreanomecon hylomecoides, Corylopsis gotoana var. coreana, Viola albida. Based on the list of Korean endemic plants, 13 taxa were recorded; Cephalotaxus koreana, Thalictrum actaefolium, Vicia nipponica, Indigofera koreana, Stewartia koreana, Weigela subsessilis, etc. Based on the list of approved for delivering overseas of plants, 5 taxa were recorded; Coreanomecon hylomecoides, Vaccinium oldhami, Paulownia coreana, Asperula lasiantha, Carex okamotoi. Specific plant species by floral region were total 21 taxa (6.2% of all 338 taxa of vascular plants); Aconitum austro-koreense, Corylopsis gotoana var. coreana in class V, Cimicifuga heracleifolia, Coreanomecon hylomecoides in class IV, 4 taxa (Stewartia koreana, Phacellanthus tubiflorus, Lonicera subhispida, etc.) in class III, Potentilla dickinsii, Viola orientalis in class II, 11 taxa (Aconitum jaluense, Angelica anomala, Erythronium japonicum, etc.) in class I. The naturalized plants in the surveyed sites were 8 families, 15 genera, 15 taxa (Phytolacca americana, Crassocephalum crepidioides, Lolium perenne, etc.) and naturalization rate was 4.4% of all 338 taxa of vascular plants.

• 한국환경복원기술학회지
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• 제17권2호
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• pp.31-48
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• 2014

This study is carried out to offer the raw data for conservation and management of tidal flat ecosystem by surveying and analysing the flora distributed in Daesong tidal flat wetland, Ahnsan-si, Gyeonggi-do coast, Korea. The results of surveying the flora were recorded as 186 taxa including 45 families, 121 genera, 170 species, 14 varieties and 2 forms. The halophytes checked around this site were 20 taxa including Atriplex gmelinii, Salicornia europaea, Suaeda glauca, Suaeda japonica, Phragmites communis, Carex scabrifolia and so forth. Polygonum bellardii that species had ecological value was the specific plant by floristic region. The growth locations of halophytes were 11 taxa of upper, 4 taxa of high tide line and 5 taxa of lower. The naturalized plants were 42 taxa including Phytolacca americana, Chenopodium glaucum, Melilotus alba, Veronica persica, Bidens pilosa, Leptochloa fusca and so forth. Because Aster subulatusand Leptochloa fusca grew a upper tidal flat wetland, they had the characteristics of halophytes. The focuses on the management of Daesong wetland were halophytes and naturalized plants. Firstly, to maintain a halophytes communities, we will sow the halophytes seeds and plant the individuals. And In-Situ conservation was applied to Polygonum bellardii habitat. Secondly, to prevent the genesis of naturalized plant, we will don't disturb around the wetland environment. The invasive alien plant, Lactuca scariola, was removed by periodic monitoring and purification activity.