• Journal of the Korean Society of Clothing and Textiles
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• v.30 no.11 s.158
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• pp.1626-1635
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• 2006

The purpose of this study was to search for comfortable protective clothing for waste incinerator workers. The experimental protective clothing came in two types: one whose outer side made use of polypropylene film, and the inner side, a non-woven rayon fabric processed with charcoal with a 10% density (CF): and one whose outer side made use of polyolefine and polyamide films, and the inner side, a non-woven polypropylene fabric (NNCF). Experiments were conducted on five healthy adult women whose average age was 21. These experiments were conducted at a climate chamber, in which the temperature and relative humidity were set below $28{\pm}1^{\circ}C\;and\;50{\pm}10%$, respectively. Measured were the rectal temperature, the skin temperature, the sweating rate, the weight loss, the heart rate, the blood pressure, the temperature, and the relative humidity of a microclimate and subjective sensation. These were measured within a period of 60 min, consisting of a 20-min stable period, a 20-min exercise period (walking exercise for 2 miles/hr on a treadmill), and a 20-min recovery period. Through this experiment, the differences between the human body's physiological reactions to CF and NNCF clothing, and the human body's comfort levels when wearing these, were determined.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.6
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• pp.2569-2574
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• 2015

Necroptosis, also known as "programmed necrosis", has emerged as a critical factor in a variety of pathological and physiological processes and is considered a cell type-specific tightly regulated process with mechanisms that may vary rather greatly due to the change of cell line. Here we used HT-29, a human colon cancer cell line, to establish a necroptosis model and elucidate associated mechanisms. We discovered that cobalt chloride, a reagent that could induce hypoxia-inducible $factor-1{\alpha}(HIF1{\alpha})$ expression and therefore mimic the hypoxic microenvironment of tumor tissue in some aspects induces necroptosis in HT-29 cells when caspase activity is compromised. On the other hand, apoptosis appears to be the predominant death form when caspases are functioning normally. HT-29 cells demonstrated significantly increased RIPK1, RIPK3 and MLKL expression in response to cobalt chloride plus z-VAD treatment, which was accompanied by drastically increased $IL1{\alpha}$ and IL6 expression, substantiating the notion that necrosis can induce profound immune reactions. The RIPK1 kinase inhibitor necrostatin-1 and the ROS scavenger NAC each could prevent necrosis in HT-29 cells and the efficiency was enhanced by combined treatment. Thus by building up a necroptosis model in human colon cancer cells, we uncovered that mechanically RIP kinases collaborate with ROS during necrosis promoted by cobalt chloride plus z-VAD, which leads to inflammation. Necroptosis may present a new target for therapeutic intervention in cancer cells that are resistant to apoptotic cell death.

• Microbiology and Biotechnology Letters
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• v.10 no.3
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• pp.211-215
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• 1982

Streptomyces griseorubiginosus var. soyoensis previously identified, produced two kinds of antifungal antibiotics, trans-cinnamamide and another new substance. The latter was identified to be a new substance of tetraene family by establishment of UV, IR, NMR, mass spectra and chemical reactions and rotatively named as Tetraene KM-A. Through an antimicrobial activity test using serial agar dilution method, Tetraene KM-A showed strong growth inhibitory activity against fungi and yeasts, but not against procaryotes tested. The inhibitory action of Tetraene KM-A on fungi was remarkably ineffective when some of sterols were added to the cultural media. $LD_{50}$ of the Tetrene KM-A to mice and rats by intravenous injection were 84.3 and 90.4 mg/kg respectively. $LD_{50}$ to mice by oral feeding was 1503mg/kg.

• Korean Chemical Engineering Research
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• v.58 no.4
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• pp.514-523
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• 2020

Plants are exposed to various types of stresses in their surroundings, and stress-resistant and regulatory proteins are produced as defense mechanisms. Abscisic acid is well known for its important role in stress signals as a phytohormone and is also involved in the physiological reactions of plants such as leaf senescence and seed dormancy. In particular, it has been found to perform a variety of functions in other biological systems, such as animals and microalgae, not plants. In this review, the biosynthesis and signaling process of abscisic acid and its function were investigated and the future prospects for the industrial application of abscisic acid in various biotechnologies, including agriculture, biomedical and industrial biotechnology, have been proposed based on study of emerging applications such as increased crop yields, disease treatment development and bioenergy production.

• Journal of the Korean Geographical Society
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• v.42 no.5
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• pp.761-768
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• 2007

Humans have been searching for more comfortable places for centuries. The comfort concept which is related with vitality in Pungsu is closest to science. The comfort concept was introduced as being based upon measurable human physiological and psychological reactions. Two classification nomograms, the comfort index and the wind effect index were identified. The Pungsu concept can be applied in America, and one of them is North Cemetery located in the town of Hampton in northeastern Connecticut not far from Storrs campus, the University of Connecticut. The human reaction to comfort not only physiologically but also individually is various in various climate region. It is important to search for more comfortable places or to make up for less comfortable places with Pungsu as the adaptive strategy for the comfort.

• Environmental Mutagens and Carcinogens
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• v.16 no.1
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• pp.13-18
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• 1996

One mechanism of free-radical production by ethanol is suggested to be through the intracellular conversion of XDH to XO by increased ratio of NADH to NAD. The major mechanism for physiological compensation of cytosolic NADH/NAD balance is the malate/aspartate shutfie. Therefore, it is important to develop the method to improve the efficiency of malate/aspartate shuttle in ethanol metabolism. In the present study, various amino acids and organic acid involved in the shuttle were tested for their functional efficiency in modulating shuttle in the ethanol-perfused rat liver. The rate of ethanol oxidation in the liver perfused with aspartate alone or aspartate in combination with pyruvate, respectively, was increased by about 10% compared to control liver, but not in the tissues perfused with glummate, cysteine or pyruvate alone. Though glummate, cysteine and pyravate did not affect the ethanol oxidation significanfiy, they showed some suppresive effect on the ethanol-induced radical generation monitored by protein carbonylation analysis. Among the tested components, aspartate is confirmed to be the most efficient as a metabolic regulator for both ethanol oxidation and ethanol-induced oxidative stress in our perfusion system. These effects of aspartate would result from NAD recycling by its supplementation through the coupled aspartate aminotransferase/malate dehydrogenase reactions and the malate-aspartate shuttle.

• Molecular & Cellular Toxicology
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• v.5 no.4
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• pp.354-363
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• 2009

Cytotoxic Nitric oxide (NO) overproduced by inducible NO Synthase (iNOS or NOS2), which was induced in inflammatory reactions and immune responses directly or indirectly affects the functions as host defense and can cause normal tissue damage. Microarray analysis was performed to identify gene profiles of both NO-dependent and -independent transcripts in RAW 264.7 macrophages that use selective NOS2 inhibitors aminoguanidine ($100\;{\mu}M$) and L-canavanine (1 mM). A total of 3,297 genes were identified that were up- or down-regulated significantly over 2-fold in lipopolysaccharide (LPS)-treated macrophages. NO-dependency was determined in the expressed total gene profiles and also within inflammatory conditions-related functional categories. Out of all the gene profiles, 1711 genes affected NO-dependently and -independently in 567 genes. In the categories of inflammatory conditions, transcripts of 16 genes (Pomp, C8a, Ifih1, Irak1, Txnrd1, Ptafr, Scube1, Cd8a, Gpx4, Ltb, Fasl, Igk-V21-9, Vac14, Mbl1, C1r and Tlr6) and 29 geneas (IL-1beta, Mpa2l, IFN activated genes and Chemokine ligands) affected NO-dependently and -independently, respectively. This NO dependency can be applied to inflammatory reaction-related functional classifications, such as cell migration, chemotaxis, cytokine, Jak/STAT signaling pathway, and MAPK signaling pathway. Our results suggest that LPS-induced gene transcripts in inflammation or infection can be classified into physiological and toxic effects by their dependency on the NOS2-mediated NO release.

• Journal of the Korean Society of Clothing and Textiles
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• v.23 no.1
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• pp.30-41
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• 1999

This study was conducted to verify the advantages of Gal-Ot. Gal-Ot is defined the clothings dyed with persimmon juice. Firstly the physical properties of fabrics were examined. Secondly the wear tests were conducted. The wear tests were performed in climatic chamber controlled 3$0^{\circ}C$ air temperature 50$\pm$5% R.H and 0.2m/s air movement. Four women subjects participated in this experiment and 4 times experiments were performed per one subject. The expeimental schedule was planned following 4 steps that is sedentary posture during 30 minutes-walking the 5。slope treadmill by 70m/min during 20 minutes-sedentary posture during 20 minutes-standing posture on toward the blowing wind during 10 minutes. The results obtained is as folows : The insulation of fabric was increased with dyeing with persimmon juice. The air permeability of fabric was remarkably increased with dyeing with persimmon juice. The mean skin temperatures of subjects were apt to be higher in wearing Gal-Ot than undyed clothing. The clothing micro temperature of subjects were slightly inclined to become lower in wearing Gal-Ot than undyed clothing, The clothing micro humidity was decreasee when: the subjects were gal-Ot than wore undyed clothing, And then it brought about more comfortable on wear sensation. The differences of physiological reactions were not consistent between clothing types except for sweat rate on the back. This result may be attributed the to complex experimental schdules consisted of four steps and to a little difference between open type and close type. However I consider that the clothing type of Gal-Ot is suitable for open type because Gal-Ot is summer wear for blocking the sultry heat.

• Research in Plant Disease
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• v.15 no.1
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• pp.36-40
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• 2009

This research was accomplished to screen the cultivar which is suitable in organic cultivation of the broccoli having good horticultural characteristics and the resistance to black rot. Thirteen cultivars of the broccoli were planted at Gangwon alpine areas of Pyeongchang county and Gangneung city and investigated the horticultural characteristics in 2006. Everybro, Tamsuerun, Nockjae, and Pilgrim had good horticultural characteristics in all two experimental fields. The resistance and susceptible reactions regarding the black rot of broccoli cultivars were different from according to two fields. This result means that physiological race of black rot pathogen distribute in Gangwon alpine areas. Everybro and Nockguck were resistance to black rot in all two experimental fields. Also these two cultivars had good horticultural characteristics. We recommend that Everybro and Nockguck will be suitable in organic cultivation.

• Toxicological Research
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• v.24 no.4
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• pp.281-287
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• 2008

Impairment of hepatic metabolism of sulfur-containing amino acids has been known to be linked with induction of liver injury. We determined the early changes in the transsulfuration reactions in liver of rats challenged with a toxic dose of $CCl_4$ (2 mmol/kg, ip). Both hepatic methionine concentration and methionine adenosyltransferase activity were increased, but S-adenosylmethionine level did not change. Hepatic cysteine was increased significantly from 4 h after $CCl_4$ treatment. Glutathione (GSH) concentration in liver was elevated in $4{\sim}8$ h and then returned to normal in accordance with the changes in glutamate cysteine ligase activity. Cysteine dioxygenase activity and hypotaurine concentration were also elevated from 4 h after the treatment. However, plasma GSH concentration was increased progressively, reaching a level at least several fold greater than normal in 24 h. ${\gamma}$-Glutamyltransferase activity in kidney or liver was not altered by $CCl_4$, suggesting that the increase in plasma GSH could not be attributed to a failure of GSH cycling. The results indicate that acute liver injury induced by $CCl_4$ is accompanied with extensive alterations in the metabolomics of sulfurcontaining amino acids and related substances. The major metabolites and products of the transsulfuration pathway, including methionine, cysteine, hypotaurine, and GSH, are all increased in liver and plasma. The physiological significance of the change in the metabolomics of sulfur-containing substances and its role in the induction of liver injury need to be explored in future studies.