• Title/Summary/Keyword: Physiological Response

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Drought and salinity stress response in wheat: physiological and TaNAC gene expression analysis in contrasting Egyptian wheat genotypes

  • El-Moneim, D. Abd;Alqahtani, Mesfer M.;Abdein, Mohamed A.;Germoush, Mousa O.
    • Journal of Plant Biotechnology
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    • v.47 no.1
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    • pp.1-14
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    • 2020
  • Drought and salinity are significant stressors for crop plants, including wheat. The relationship between physiological mechanisms and gene expression is important for stress tolerance. NAC transcription factors (TFs) play vital roles in abiotic stress. In this study, we assessed the expression of four TaNAC genes with some physiological traits of nine Egyptian wheat genotypes under different concentrations of PEG and NaCl. All the physiological traits that we assessed declined under both stress conditions in all genotypes. In addition, all the genes that we measured were induced under both stress conditions in young leaves. Shandaweel 1, Bani Seuf 7, Sakha 95, and Misr 2 genotypes showed higher gene expression and were linked with a better genotypic performance in physiological traits under both stress conditions. In addition, we found an association between the expression of NAC genes and physiological traits. Overall, NAC genes may act as beneficial markers for selecting for genotypic tolerance to these stress conditions in wheat.

The Changes of Psychological and Physiological Emotional Responses According to Change of the Index of Predicted Mean Vote (PMV) due to Air Conditioning Types (공조방식에 의한 예상 온열감 반응(PMV) 변화에 따른 심리/생리적 감성반응의 변화)

  • Kim, Bo-Seong;Min, Yoon-Ki;Min, Byung-Chan;Kim, Jin-Ho
    • Science of Emotion and Sensibility
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    • v.14 no.4
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    • pp.645-652
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    • 2011
  • This study examined changes of both psychological and physiological emotional responses according to change of the PMV (predicted mean vote) in the heating and the cooling air conditions. For this purpose, the changes of PMV were induced by the heating and cooling operations of the HVAC (heating, ventilation, and air conditioning) systems. In addition, positive/negative and arousal/relaxation were measured as the participant's psychological emotional responses, and HR (heart rate) was measured as the participant's physiological emotional responses. As a result, in same range of the PMV, both psychological and physiological emotional-responses were changed by air conditioning. It is suggested that occupant's emotional responses would depend on the operational conditions of heating and cooling in indoor thermal environments, and both psychological and physiological emotional response should be considered when occupants try to match the indoor thermal environments to their thermal expectations.

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Study on CsRCI2D and CsRCI2H for improvement of abiotic stress tolerance in Camelina sativa L.

  • Lim, Hyun-Gyu;Kim, Hyun-Sung;Kim, Jung-Eun;Ahn, Sung-Ju
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.196-196
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    • 2017
  • Oilseed crop Camelina (Camelina sativa L.) is a suitable for biodiesel production that has high adaptability under low-nutrient condition like marginal land and requires low-input cost for cultivation. Enhanced abiotic stress tolerance of Camelina is very important for oil production under the wide range of different climate. CsRCI2s (Rare Cold Inducible 2) are related proteins in various abiotic stresses that predicted to localized at plasma membrane (PM) and endoplasmic reticulum (ER). These proteins are consist of eight-family that can be divided into tail (CsRCI2D/E/F/G) and no-tail (CsRCI2A/B/E/H) type of C-terminal. However, it is still less understood the function of C-terminal tail. In this study, CsRCI2D/H genes were cloned through gateway cloning system that used pCB302-3 as destination vector. And we used agrobacterium-mediated transformation system for generation of overexpression (OX) transformants. Overexpression of target gene was confirmed using RT-PCR and segregation ratio on selection media. We analyzed physiological response in media and soil under abiotic stresses using CsRCI2D and CsRCI2H overexpression plant. To compare abiotic stresses tolerance, wild type and CsRCI2D/H OX line seeds were sown on agar plate treated with various NaCl and mannitol concentration for 7 days. In the test of growth rate under abiotic stress on media, CsRCI2H OX line showed similar to NaCl and mannitol stress. In the other hand, CsRCI2D OX line showed to be improved stress tolerance that especially increased in 200mM NaCl but was similar on mannitol media. In greenhouse, WT and CsRCI2D/H OX lines for physiological analysis and productivity under abiotic stresses were treated 100, 150, 200mM NaCl. Then it was measured various parameters such as leaf width and length, plant height, total seed weight, flower number, seed number. CsRCI2H OX line in greenhouse did not show any changes in physiological parameters but CsRCI2D OX line was improved both physiological response and productivity under NaCl stress. Among physiological parameters of CsRCI2D OX line under NaCl stress, leaf length and width were observed shorter than WT but it were slightly longer than WT in 200mM NaCl stress. Furthermore, total seed weight of CsRCI2D OX line under stress displayed to decrease than WT in normal condition, but it was gradually raised with increasing NaCl stress then more than WT relatively. These results suggested CsRCI2D might be contribute to improve abiotic stress tolerance. However, function of CsRCI2H is need to more detail study. In conclusion, overexpression of CsRCI2s family can generate various environmental stress tolerance plant and may improve crop productivity for bio-energy production.

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The Effect of Recorded Mother's Voice on the Body Weight and Physiological Reactions of the Premature Infants (신생아 중환자실 미숙아에게 엄마목소리 제공이 미숙아의 체중과 생리적 반응에 미치는 효과)

  • Kang, In-Soon;Kim, Young-Hee
    • Journal of Korean Biological Nursing Science
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    • v.13 no.2
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    • pp.94-101
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    • 2011
  • Purpose: The purpose of this study is to examine effects of Recorded Mother's Voice on weight and physiological reactions to the premature infants. Methods: This study was conducted on the nonequivalent control group pre-posttest quasi-experimental design and the subject group was the premature infants who were admitted in Neonatal Intensive Care Unit in Pusan National University Hospital. Total 50 infants, divided into two groups 25 experimental and control were participated in the study. The data were analyzed using frequency, %, chi-square test, t-test between the two groups. Results: The results of this study were as followingsfollowings; In the physical response(heart rate, respiration rate, pulse oximetry saturation), there were significant statistical difference between two groups. Conclusion: In short, it turned out that the intervention of the recorded mother's voice had some significance as nursing intervention with positive impacts. Such as improving infant's growth and stablizing their physical response. By providing the effectiveness of such intervention, the study will further provide the evidence-based information in developing the practice of pediatric nursing.

Depurination of dA and dG Induced by 2-bromopropane at the Physiological Condition

  • Thapa, Pritam;Sherchan, Jyoti;Karki, Radha;Jeong, Tae-Cheon;Lee, Eung-Seok
    • Biomolecules & Therapeutics
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    • v.15 no.4
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    • pp.224-229
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    • 2007
  • Depurination, the release of purine bases from nucleosides by hydrolysis of the N-glycosidic bond, gives rise to alterations of the cell genome. Although, cells have evolved mechanisms to repair these lesions, unrepaired apurinic sites have been shown to have two biological consequences: lethality and base substitution errors. 2-Bromopropane (2-BP) is used as an intermediate in the synthesis of pharmaceuticals, dyes, and other organics. In addition, 2-BP has been used as a cleaning solvent in electronics industry. But, 2-BP was found to cause reproductive and hematopoietic disorders in local workers exposed to it. We observed massive depurination after incubation of 2'-deoxyadenosine (dA) and 2'-deoxyguanosine (dG) with the excess amount 2-BP at the physiological condition (pH 7.4, $37^{\circ}C$), which were analyzed by HPLC and LC-MS/MS. In addition, time and dose response relationship of depurination in dA and dG induced by 2-BP at the physiological condition were investigated.

The Effects of Sodium Chloride on the Physiological Characteristics of Listeria monocytogenes

  • Choi, Kyoung-Hee;Yoon, Yohan
    • Food Science of Animal Resources
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    • v.33 no.3
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    • pp.395-402
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    • 2013
  • Sodium chloride is used to improve various properties of processed meat products, e.g., taste, preservation, water binding capacity, texture, meat batter viscosity, safety, and flavor; however, many studies have shown that sodium chloride increases the resistance of many foodborne pathogens to heat and acid. Listeria monocytogenes has been isolated from various readyto- eat (RTE) meat and dairy products formulated with sodium chloride; therefore, the objective of this paper was to review the effects of sodium chloride on the physiological characteristics of L. monocytogenes. The exposure of L. monocytogenes to sodium chloride may increase biofilm formation on foods or food contact surfaces, virulence gene transcription, invasion of Caco-2 cells, and bacteriocin production, depending on L. monocytogenes strain and serotype as well as sodium chloride concentration. When L. monocytogenes cells were exposed to sodium chloride, their resistance to UV-C irradiation and freezing temperatures increased, but sodium chloride had no effect on their resistance to gamma irradiation. The morphological properties of L. monocytogenes, especially cell elongation and filament formation, also change in response to sodium chloride. These findings indicate that sodium chloride affects various physiological responses of L. monocytogenes and thus, the effect of sodium chloride on L. monocytogenes in RTE meat and dairy products needs to be considered with respect to food safety. Moreover, further studies of microbial risk assessment should be conducted to suggest an appropriate sodium chloride concentration in animal origin foods.

AUTONOMIC MECHANISMS OF AN ACUTE STRESS RESPONSE DURING WORD RECOGNITION TASK PERFORMANCE WITH INTENSE NOISE BACKGROUND (백색소음하의 단어재인검사 수행에 따른 자율신경계 스트레스 반응)

  • ;;;Estate Sokhadze
    • Proceedings of the Korean Society for Emotion and Sensibility Conference
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    • 1999.03a
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    • pp.127-132
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    • 1999
  • Cardiovascular, respiratory and electrodermal responses to acute stress episodes modeled by combined presentation of intense white noise and performance of word recognition task with noise background were studied in 15 college students. Experimental procedure consisted in sessions with white noise, word recognition task presentation with noise background and test with noise background. Recorded physiological variables were analyzed in terms of their sensitivity to detect activation of sympathetic and parasympathetic branches of autonomic nervous system and thus reflect autonomic arousal level during shout-term stress-inducing experimental manipulations. It was shown that performance of effortful mental task with noise background elicited significant physiological responses typical for active coping behavior, namely electrodermal arousal and increased cardiovascular activity. this response profile was more profound as compared to white noise only or attending task in noise background. However, all physiological responses were mostly phasic, without long-term tonic changes, since almost all variables recovered to their initial baseline levels, suggesting that dominant autonomic mechanisms in transient acute stress episodes were of parasympathetic nature (withdrawal in stress with subsequent activation in restoration period), while sympathetic contribution was not long-lasting. Nevertheless, increased number of stressors and their longer exposure may result in higher profile of tonic sympathetic arousal and reduced functional role of vagal mechanisms in autonomic balance regulation.

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A Study on the Effects of Heat Stress on Feedlot Environment and Productivity of Dairy Cattle (고온 환경이 젖소의 생산성 및 축사환경에 미치는 영향 연구)

  • Kim, Byul;Lim, Joung-Soo;Cho, Sung-Back;Hwang, Ok-Hwa;Yang, Seung-Hak
    • Journal of Animal Environmental Science
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    • v.20 no.2
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    • pp.63-68
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    • 2014
  • Environmental heat stress by global warming has a severe effect on the productivity of livestock and, in particular, on that of dairy cattle. Heat stress during high temperature environment directly and indirectly affects milk yield, milk quality and physiological response. This study was conducted to investigate the effects of heat stress on productivity and physiological responses of livestock. Temperature-humidity data logger were established inside the feedlot for measuring real time changes in the feedlot environment. Milk was collected every day for analysing the productivity of dairy cattle. Blood sample and respiration of dairy cattle were collected once in a week for investigating the physiological response factors. Blood component concentration associated with lipolysis metabolism and milk production showed change during tropical night period. Temperature humidity index (THI) of a specific location inside the feedlot showed continuously high levels.

Analysis of Psychological, Physiological(Heart Rate Variability) Response & Influence Consequent on Green Light Brightness (녹색광 밝기에 따른 심리·생리(심박변이) 반응 및 영향 분석)

  • Jung, Chan-Ung;Im, O-Youn;Lee, Jin-Sook
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.30 no.3
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    • pp.9-20
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    • 2016
  • This study compared the effect of green light consequent on maximum brightness 85 lx and intial brightness 15 lx, and its accompanying psychological, physiological response. 1) As a result of psychology(general stress evaluation, Symptom Checklist-90-Revision) evaluation, all stress evaluation items mostly showed a decrease, and they are comparatively positively evaluated in minimum brightness 15 lx comparing to maximum brightness 85 lx. 2) Physiological (Heart Rate Variability) evaluation result was included in stable range in all cardiovascular system variables after light stimulus in time of comparison with the standard range; in addition, the Heart Rate Variability evaluation result was found to be stable in all cardiovascular system variables except Low Frequency in minimum brightness 15 lx comparing to maximum brightness 85 lx. 3) Green light was found not to appear as a stimulating factor in the human body in time of space projection, being found to be effective in stability of mind and body, and reduction in stress. Thus, it was found that green light functioned as an emotional, relaxing tranquilizer by working on the sympathetic nervous system.

Physiological Roles of Bacillus subtilis thiol peroxidase gene in response to oxidative stress (산화적 스트레스에 대한 Bacillus subtilis의 thiol peroxidase 유전자의 생리적인 기능)

  • Kim, Ha-Kun;Kim, Sung-Jin
    • The Journal of Natural Sciences
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    • v.15 no.1
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    • pp.57-67
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    • 2005
  • In Order to investigate the physiological role of thiol peroxidase in Bacillus subtilis, a thiol peroxidase (btpx) knock-out mutant was generated by homologous recombination. The growth of btpx knock-out mutant in aerobic condition showed a similar pattern with that of wild type of Bacillus subtilis 168/ But btpx knock-out mutant showed a retarded growht in response to oxidative stress such as $H_2O_2$, cumene hydroperoxide (CHP) treatments.

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