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http://dx.doi.org/10.4062/biomolther.2007.15.4.224

Depurination of dA and dG Induced by 2-bromopropane at the Physiological Condition  

Thapa, Pritam (College of Pharmacy, Yeungnam University)
Sherchan, Jyoti (College of Pharmacy, Yeungnam University)
Karki, Radha (College of Pharmacy, Yeungnam University)
Jeong, Tae-Cheon (College of Pharmacy, Yeungnam University)
Lee, Eung-Seok (College of Pharmacy, Yeungnam University)
Publication Information
Biomolecules & Therapeutics / v.15, no.4, 2007 , pp. 224-229 More about this Journal
Abstract
Depurination, the release of purine bases from nucleosides by hydrolysis of the N-glycosidic bond, gives rise to alterations of the cell genome. Although, cells have evolved mechanisms to repair these lesions, unrepaired apurinic sites have been shown to have two biological consequences: lethality and base substitution errors. 2-Bromopropane (2-BP) is used as an intermediate in the synthesis of pharmaceuticals, dyes, and other organics. In addition, 2-BP has been used as a cleaning solvent in electronics industry. But, 2-BP was found to cause reproductive and hematopoietic disorders in local workers exposed to it. We observed massive depurination after incubation of 2'-deoxyadenosine (dA) and 2'-deoxyguanosine (dG) with the excess amount 2-BP at the physiological condition (pH 7.4, $37^{\circ}C$), which were analyzed by HPLC and LC-MS/MS. In addition, time and dose response relationship of depurination in dA and dG induced by 2-BP at the physiological condition were investigated.
Keywords
depurination; 1-bromopropane; 2-bromopropane; time and dose response relationship;
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