• Journal of Embryo Transfer
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• v.25 no.4
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• pp.259-262
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• 2010

Optimization of the preimplantation mammalian embryo culture condition was widely focused on refining medium composition under the name of chemically defined media. However, recent research revealed that the alteration of physical environment can be a crucial factor to a successful embryo development. In this study, under the same embryo density, a novel culture device named oil-free micro tube culture (MTC) system was evaluated using porcine parthenogenetic embryos. The activated oocytes were placed into the 0.2 ml thin-wall flat cap PCR tube and cultured to the blastocyst stage. As a preliminary step, embryo density and culture medium volume were optimized under a standard drop culture system. The optimal embryo density range for in vitro culture was 0.5 embryos per ${\mu}l$ in $20\;{\mu}l$ drop (20.5%) and 1.0 embryos per ${\mu}l$ in $10\;{\mu}l$ drop (20.6%). Based on these results, we compared drop culture system and 'MTC' system in terms of the developmental rate to the blastocyst stage. In $20\;{\mu}l$ medium volume, the 'MTC' system showed similar blastocyst formation rate when compared with drop culture system (20.2% versus 20.5%, respectively) while the 'MTC' system showed lower blastocyst formation rate than drop culture system in $10\;{\mu}l$ one (12.7% versus 20.0%, respectively). Therefore the $20\;{\mu}l$ MTC system may be an alternative incubation system for short-distance embryo transport without carrying the $CO_2$ incubator and this provides novel embryo culture device to clinical veterinary embryologists.

• International Journal of Internet, Broadcasting and Communication
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• v.12 no.4
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• pp.156-165
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• 2020

We study empirically analyzed the final 216 copies of the responses from SME organization members in Seoul and Gyeonggi regions using statistical programs of SPSS24.0 and AMOS 24.0. In other words, the effect of job satisfaction on the organizational culture and job environment characteristics perceived by members of the organization, and the relationship between job satisfaction and job performance is shown as follows. First, the organizational culture of consensus, development, hierarchy, and rationality was statistically significant in job satisfaction. Therefore, the hypothetical one-man organizational culture was adopted by showing a positive (+) effect on job satisfaction. Second, job environment characteristics such as autonomy in job performance, compensation system, physical environment, and human environment had a positive effect on job satisfaction. That is, the job environment characteristics of hypothesis 2 were adopted as a positive (+) influence relationship on organizational satisfaction. Third, job satisfaction was expressed as a positive (+) influence relationship with job performance, so hypothesis 3 was adopted. As a result of this study, the higher the organizational member's perception of organizational culture and job environment characteristics is, the higher the job satisfaction is, and the improvement in job satisfaction is meaningful in that it provides theoretical and practical implications that indicate job performance.

• Journal of the Korean housing association
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• v.25 no.5
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• pp.33-41
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• 2014

Today, modern society pursues the paradigm of creative city and regeneration. Cultural regeneration of revitalizing locality by utilizing culture catches attention, is a major approach. Public arts is considered as a catalyst of cultural regeneration, and it inspires vitality to declined districts. Gamcheon Culture Village, Busan has accelerated propagation of revitalization only in recent couple of years, and it has been developing with residents' participation continuously. The purpose of this research is to analyse the revitalizing effects of public arts introduction to Gamcheon Culture Village regeneration. In specific, social, cultural, economical, physical and environmental revitalization effects of the village were defined. The research method is questionnaire survey and 320 reponses from residents and visitors were collected. Data were statistically analyzed with SPSS18.0. The effects of public arts introduction to Gamcheon Culture Village regeneration are positive overall. However, according to the districts of the village, having diversity of art projects in both quantity and quality, the perceptions were different. Regeneration with utilizing public arts for revitalizing declined district, the Gamcheon case shed light on the future. The research indicated the value of public arts and some strategies of allocating art projects.

• Journal of Environmental Science International
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• v.14 no.12
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• pp.1163-1170
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• 2005

The microbial adsorption characteristics of two different media for biological treatment were studied using attached diverse microbes onto activated carbon and ceramic. The results in the experiments of the characteristics of physical adhesion on two different media with addition of high and low concentrated substrate in the culture were observed that the efficient of adhesion onto F-400 activated carbon was higher over that of ceramic due to the surface area of media. The irradiation treatment by ultrasonication with 400 W power and 3 min retention time on the media without addition substrate conditions and subsequent mixing throughly the culture showed the highest efficiency of cell detachment on the media. Three different microbes, P. ovalis, A calcoaceticus, and B. subtillis were used for the study of the characteristics of microbial adhesion on the media. p ovalis showed the highest adhesion capability while B. subtillis showed the lowest capability adhesion onto media either addition of substrate in the culture. The mixed bacterial culture showed $10\%$ lower removal efficiency of DOC in the low concentrated substrate culture compared to the single pure culture. Whileas, it did not show significant difference between two cultures at high concentrated substrate. It was also observed same population density of microorganism by counting of microbes adhered to microbial media with an ultrasound treatment.

• Journal of Korean Library and Information Science Society
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• v.21
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• pp.291-323
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• 1994

The Result of the study can be summarized as follows : (1) Korean culture and public library look extremely wasted equally. Judging from this fact, I believe that it is impossible for public library can contribute to development of Korean culture. (2) But, public library can contribute to development of culture in the cultural environment that mental desire is prior to physical wants. This cultural environment can be built up with reading of good books. Therefore, if class to reading education and diverse reading education programs can be activated in a large portion of the nationwide public libraries and the majority of a local resident are capable of improving their creativity power, thinking power and imaginative faculty, public library can contribute to produce such cultural circumstances. (3) Quality, culture, a human and mind receive mu6 respect in Korean Society as advanced civil, democratic and industrial society in the future. The Society will be influenced by reason and rational culture will be suitable to this society. Creativity power, thinking-power, imaginative faculty, intelligence and power of information management are demanded to the greatest degree for the development of idea and new technology in the society under the control of rational culture. So, lifelong education with library use is the most fruitful and inexpensive way for the improvement of these powers.

• The Korean Journal of Health Service Management
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• v.14 no.1
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• pp.177-194
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• 2020

Objectives: This study aims to identify the drinking environment and drinking culture in an area with high drinking rates in order to provide the basis for the development of a drinking intervention program. Methods: Forty-six local experts and residents participated in focus group interviews that mainly asked questions about the drinking environment in the community, the culture and behavior of drinking, and the community efforts to reduce the drinking rates. The interviews of four groups were transcribed and analyzed. Results: Drinking environments and cultures were categorized into the following five themes: high physical accessibility to drinking, type of housing and long duration of stay in the same region, drinking-friendly culture and daily life events, various reasons and patterns of drinking, and lack of health (education) programs. Conclusions: Community efforts are required to make the residents aware of how the local environment is related to the high drinking rates in their community. Further, the study underlines the need for the community to make efforts to create an environment where drinking rates are low, and foster a diverse leisure culture.

• Journal of the Korean Society of Clothing and Textiles
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• v.12 no.1 s.26
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• pp.13-26
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• 1988

As the culture develops, the body of children nowadays improves, but their physical fitness is weaker than that of children in the past. If physical fitness strengthens through clothing, this must be the easiest and the most efficient long-range training method. To find out the solutions on the above mentioned problems through clothing, this study was made for the analysis of the correlations of clothing weight and physical fitness. The children's endurance upon cold can be explained by the physical fitness, and has relation with the clothing insulation. Therefore, motor ability was measured as an index of physical fitness and clothing weight was measured as an index of insulation. This observation was made in Spring and Fall, 1985, in which 339 kindergartners and their mothers were the subject. The results of the study were as follows: Physical fitness of children decreased in proportion to the clothing weight, and especially this can be seen in boys rather than in girls, and in Fall rather than in Spring. In case of the children who spent more time outdoors rather than indoors, who played in larger space, and who didn't have meat frequently, it turned out that their clothing weight was surprisingly lessened and their physical fitness was highly food.

• Journal of Korea Game Society
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• v.11 no.2
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• pp.93-104
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• 2011

For development of physical interactive game recognized as next generation game, this study examines the present situation of physical interactive games and analyzes the problem that game contents lack development compared to the hardware. This paper firstly divides the problem of physical interactive game contents into three parts: demand of the contents and biased game genre, low level of game difficulty, and limited use and/or misuse of the motion controller. Then, the possible solutions for each part are suggested afterward. As the discussion on the development methods in physical interactive game will also develop and therefore, as the next generation game, physical interactive game will play a positive role in th growth of game industry as well as game culture.

• Physical Therapy Korea
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• v.9 no.3
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• pp.93-99
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• 2002

Traditionally, ultraviolet (UV) has been used for treating the pressure sore and skin wound. The effects of UVA and UVB radiation on disinfection have been reported. The purpose of this study was to examine the effectiveness of UVC radiation on disinfection of Escherichia coli, Staphylococcus aureus, Salmonella typhimurium in vitro. Three bacterium were radiated by UVC (250 nm, 20 seconds) and incubated at $37^{\circ}C$ for 24 hours at the agar culture medium. Kill rates of all three bacterium were 99.9%. UVC radiated on three kinds of bacterium for 30 or 60 seconds. Kill rates were 99.9% both 30 and 60 seconds. This data suggests that UV light at 250 nm could be a useful method to minimize infection and shorten healing time in pressure sore and skin wound condition.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2012.10a
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• pp.1000-1002
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• 2012

The material with superior biocompatibility and physical-chemical stability is required to fabricate high sensitive biosensors. Many kinds of biomaterials have been evaluated to apply for bioindustry. Recently, carbon based diamond and graphene thin films have been focal pointed as bio applications and their possibility is partially evaluated. Diamond thin film has many advantages for electrochemical and biological applications, such as wide potential window (3.0~3.5V), low background current and chemical-physical stability. And graphene film has many advantages as biomaterial, chemical-physical stability and conductivity. In this work, we have cultured human nerve cell (SH-SY5Y) on the nanocrystalline diamond, mirocrystalline diamond, graphene film and cell culture dish. We use MTT assay to evaluate the characteristics of cell culture on the substrates. As a result, nerve cell is well cultured on the carbon based diamond and graphene films as similar as cell culture dish. We expect that carbon materials have been applied for bioindustry such as biosensors.