• ALGAE
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• v.27 no.3
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• pp.175-187
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• 2012

Hainan is the second largest and southernmost island of China, and it harbors many species of the family Gracilariaceae. In this study, we conducted molecular and morphological analyses of gracilarian specimens collected from Hainan Island, to clarify their taxonomic identity and phylogenetic relationships. Five species of Gracilariaceae in Hainan were determined through maximum likelihood and Bayesian analysis of rbcL sequence data. Based on the phylogenetic trees and morphology, Hainan specimens were identified as three species of Gracilaria, one species of Gracilariopsis, and one species of Hydropuntia, namely, G. salicornia, G. tenuistipitata, Gracilaria sp., Gp. bailiniae and H. edulis. This is the first report of the molecular phylogeny of Gracilariaceae on Hainan Island, and it helps to clarify the taxonomy and distribution of gracilarian species in the Asia-Pacific region.

• ALGAE
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• v.31 no.4
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• pp.341-349
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• 2016

The red algal genus Amalthea was first described from New Zealand with one species, A. freemaniae. We discovered a new species of Amalthea from Korea, Amalthea rubida sp. nov., and described it based on morphology and rbcL sequences. A. rubida is characterized by a foliose, membranous and soft thallus having loosely arranged anticlinal medullary filaments, much like A. freemaniae, except for a difference of the cortex thickness in the basal part of vegetative structure. In the rbcL phylogenetic analyses, A. rubida formed a clade with A. freemaniae from New Zealand and Halymenia abyssicola from Mexico. A. rubida showed 5.7% interspecific divergence to A. freemaniae and 4.7% to H. abyssicola. Our discovery of A. rubida expands the distribution of Amalthea from the oceanic southwestern Pacific into the continental northwestern. A targeted global study is needed to fully reveal the species diversity and phylogenetic relationships of the genus Amalthea and members of the order Halymeniales.

• Journal of Photoscience
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• v.8 no.2
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• pp.55-60
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• 2001

Ginseng (Panax genus) is one of the most medicinally important genera and consists of highly regarded medicines. Among the species of Panax, the ginseng species is widely known to have most medicinal quality. P. ginseng has 3 varieties, Jakyung, Chunggyung and Hwangsook, discovered in nature with different colors of stem and fruit, Jakyung has two cultivars, Yunpoong and Chunpoong. Rigorous phylogenetic analysis of these varieties and cultivars has been conducted with sequencing of rDNA region. The sequences of ITS1, ITS2 of every varieties and cultivars within P. ginseng were identical. The sequence of 5.8S rDNAs of Hwangsook variety were different from the sequences of 5.8S rDNAs of others by only one base pair at nucleotide position 14. In phylogenetic analysis and predicted RNA secondary structure study, it is assumed that evolution has proceeded from Hwangsook to other varieties. recently.

• Journal of Microbiology and Biotechnology
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• v.18 no.1
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• pp.23-27
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• 2008

Self-splicing group I introns in tRNA anticodon loops have been found in diverse groups of bacteria. In this work, we identified $tRNA^{fMet}$ group I introns in six strains of marine Synechococcus elongatus. Introns with sizes around 280 bp were consistently obtained in all the strains tested. In a phylogenetic analysis using the nucleotide sequence determined in this study with other cyanobacterial $tRNA^{fMet}$ and $tRNA^{Leu}$ intron sequences, the Synechococcus sequence was grouped together with the sequences from other unicellular cyanobacterial strains. Interestingly, the phylogenetic tree inferred from the intronic sequences clearly separates the different tRNA introns, suggesting that each family has its own evolutionary history.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2000.10a
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• pp.118-124
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• 2000

Zn-resistant Methylosobacterium strains were isolated from several non-polluted soil samples collected in all over Japan. Zn-resistant Methylosobacterium strains were predominantly detected in all soil samples and they were also characterized as a UV-tolerant bacteria. The MIC test revealed that the isolates have high zinc resistance in comparison with that of reference Methylosobacterium strains obtained from culture collections. The 16S rDNA-based phylogenetic analysis showed that all strains were divided into two clusters designated as cluster A and cluster B in the present study. All isolates were distributed only in the cluster A. The phylogenetic clustering also well coincided with the differences in the pattern of carbon source utilization.

• The Korean Journal of Mycology
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• v.44 no.4
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• pp.263-270
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• 2016

Freshwater is a diverse and complex environment for fungi and provides several types of habitat including plant litter, sediment, and carcasses. We collected plant litter from the main stream and branch streams of Nakdong River, Sohancheon in Samcheok, and Geumoreum in Jeju. From several samples of plant litter, we isolated 8 fungal strains belonging to 4 Montagnulaceae species unrecorded in Korea: Paraconiothyrium archidendri, Paraphaeosphaeria sporulosa, Paraphaeosphaeria michotii, and Paraphaeosphaeria viridescens. These fungi were identified by phylogenetic analysis using the internal transcribed spacer (ITS) region and examination of morphological characteristics. Montagnulaceae is known as one of the families in Pleosporales and includes coniothyrium-like fungi. In this study, we described phylogenetic analysis and mycological characteristics of these species, and this is the first report of these taxa in Korea.

• Mycobiology
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• v.49 no.4
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• pp.363-375
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• 2021

Penicillium species are known to be ubiquitous environmental saprophytes. In the survey of diversity of genus Penicillium, seven new records of Penicillium species belonging to section Lanata-Divaricata were isolated from freshwater and soil samples collected from different locations in Korea. Based on morphological characteristics and multilocus phylogenetic analysis of the rDNA internal transcribed spacer region (ITS), β-tubulin (BenA), and calmodulin (CaM) genes, the isolated strains were identified as P. annulatum, P. camponotum, P. echinulonalgiovense, P. globosum, P. limosum, P. onobense, and P. yunnanense, respectively. This study presents detailed phylogenetic analyses and morphological descriptions of these species that contribute to section Lanata-Divaricata in Korea.

• The Korean Journal of Mycology
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• v.49 no.2
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• pp.139-153
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• 2021

The Inocybaceae is a family of ectomycorrhizal fungi belonging to the Agaricales order. Most species in this family produce secondary metabolites, such as muscarine, psilocybin, and other compounds. In the present study, we conducted taxonomic studies on the Inocybaceae based on morphological and molecular phylogenetic analyses for the first time in Korea. As a result, seven species of Inocybaceae were newly recorded: Inocybe caroticolor, I. fuscidula, I. grammatoides, I. latibulosa, I. stellata, Inosperma shawarense, and Mallocybe malenconii. Among them, the genera Inosperma and Mallocybe were described for the first time in Korea. Relevant morphological characteristics, phylogenetic trees, and photographs are provided.

• Mycobiology
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• v.47 no.2
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• pp.173-179
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• 2019

Cladophialophora is characterized by simple hyphomycetes with brown hyphae that give rise to branched chains of pale brown conidia and shows affinities with the Herpotrichiellaceae. A fungal strain belonging to the genus Cladophialophora was isolated from soil in Daegu, Korea. This strain produces numerous greenish to dark black lanose aerial mycelia with hair like structures. It is morphological similar to C. chaetospira, C. inabaensis, and C. multiseptata; however, the conidiophores and conidia sizes of the newly isolated strain (KNU16-032) are clearly different from them. The novelty of the strain was also confirmed based on phylogenetic analysis using the data sets of the internal transcribed spacer region of and the partial sequence of 28S ribosomal DNA region along with the cultural characteristics. Because morphological observations and phylogenetic analysis indicated that the strain is distinct from previously known Cladophialophora species, we propose this species as a new species Cladophialophora lanosa sp. nov., and provide the detailed descriptions in this study.

• Research in Plant Disease
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• v.27 no.3
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• pp.107-114
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• 2021

During a disease survey on weeds and minor cultivated crops in Korea, a brown leaf spot disease was observed on Sonchus asper. Leaf lesions were round or irregular in shape, and grayish brown to brown with a purple margin. In severe infection, lesions enlarged and coalesced, resulting in blighting of the leaves. The isolates from these leaf lesions were identified as Alternaira sonchi based on morphological characteristics and phylogenetic analyses of Internal transcribed spacer region, Alternaria allergen a1, glyceraldehyde 3-phosphate dehydrogenase, RNA polymerase II, and translation elongation factor genes. This study provides a comprehensive description of the morphological characteristics and phylogenetical traits of A. sonchi causing brown leaf spot on S. asper in Korea.