• 식물분류학회지
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• 제52권3호
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• pp.127-143
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• 2022

This study was conducted to clarify the phylogenetic position and relationships of Korean Poaceae taxa. A total of 438 taxa including 155 accessions of Korean Poaceae (representing 92% and 72% of Korean Poaceous genera and species, respectively) were employed for phylogeny reconstruction. Sequence data of eight chloroplast DNA markers were used for molecular phylogenetic analyses. The resulted phylogeny was mostly concordant with previous phylogenetic hypotheses, especially in terms of subfamilial and tribal relationships. Several taxa-specific indels were detected in the molecular phylogeny, including a 45 bp deletion in rps3 (PACMAD [Panicoideae, Arundinoideae, Chloridoideae, Micrairoideae, Aristidoideae, Danthonioideae] clade), a 15 bp deletion in ndhF (Oryzeae + Phyllorachideae), a 6 bp deletion in trnLF (Poeae s.l.), and two (17 bp and 378 bp) deletions in atpF-H (Pooideae). The Korean Poaceae members were classified into 23 tribes, representing eight subfamilies. The subfamilial and tribal classifications of the Korean taxa were generally congruent with a recently published system, whereas some subtribes and genera were found to be non-monophyletic. The taxa included in the PACMAD clade (especially Andropogoneae) showed very weak and uncertain phylogenetic relationships, presumably to be due to evolutionary radiation and polyploidization. The reconstructed phylogeny can be utilized to update the taxonomic positions of the newly examined grass accessions.

• 생명과학회지
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• 제13권6호
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• pp.856-864
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• 2003

원핵생물 (Procaryote)의 분류에 16S rRNA유전자가 많이 이용되어 있으나 제한된 해상력과 유전자의 수에 차이가 있는 등의 문제가 있어 이를 보완할 수 있는 새로운 생체분자를 찾고 그 분류 결과를 16S rRNA의 결과와 비교하였다. COG (Clusters of Orthologous of protein) 방법을 이용하여 43종의 미생물중에서 진핵생물을 제외한 42종의 원핵생물 (procaryote)에서만 발견되는 3종류의 COG인 Transcription elongation factor인 COG0195과 bacterial DNA primase인 COG0358 그리고 uridylate kinase인 COG0528를 구하였다. 이중 유사도와 유전자 수를 바탕으로 새로운 분류의 키로 uridylate kinase를 설정하여 분석한 결과, 같은 속 (genus)에 속하는 세균들은 아주 높은bootstrap value를 갖고 분류도에서 같은 위치에 분포하고 고세균 (Archaebacteria) 내부의 응집성이 높은 등의 유사성을 보였다. 한편 alpha와 epsilon 그룹의 Proteobacteria가 분류도에서 다르게 위치하고 진정세균 (Eubacteria)의 Spi-rochaetales에 속하는 Treponema pallidum (Tpa)와 Borrelia burgdorferi (Bbu)가 고세균과 유연관계가 높게 나타나는 등 차이점도 보였다. Uridylate kinase를 이용한 분류는, 아주 높은 보존성에 의해서 생기는 16S rRNA 유전자를 이용한 문제점을 보완하여 원핵생물의 정확한 분류에 기여할 수 있을 것으로 사료되었다.

• Molecules and Cells
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• 제27권1호
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• pp.55-66
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• 2009

The phylogeny of the family Tephritidae (Diptera: Tephritidae) was reconstructed from mitochondrial 12S, 16S, and COII gene fragments using 87 species, including 79 tephritid and 8 outgroup species. Minimum evolution and Bayesian trees suggested the following phylogenetic relationships: (1) A sister group relationship between Ortalotrypeta and Tachinisca, and their basal phylogenetic position within Tephritidae; (2) a sister group relationship between the tribe Acanthonevrini and Phytalmiini; (3) monophyly of Plioreocepta, Taomyia and an undescribed new genus, and their sister group relationship with the subfamily Tephritinae; (4) a possible sister group relationship of Cephalophysa and Adramini; and (5) reconfirmation of monophyly for Trypetini, Carpomyini, Tephritinae, and Dacinae. The combination of 12S, 16S, and COII data enabled resolution of phylogenetic relationships among the higher taxa of Tephritidae.

• 한국균학회지
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• 제49권2호
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• pp.175-181
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• 2021

A fungal strain was isolated from a soil sample collected in Korea and designated as YW23-8. Based on a sequence analysis of the internal transcribed spacer (ITS) regions, the isolate was assigned to the genus Sarcopodium. Moreover, a phylogenetic analysis based on the concatenated nucleotide sequences of the ITS regions and the large subunit of the nuclear ribosomal RNA (LSU) gene showed that the strain YW23-8 occupies a distinct phylogenetic position within Sarcopodium. The isolate had significant differences from its closest neighbors, S. circinosetiferum, S. circinatum, S. macalpinei, and S. vanillae. Morphological features such as different conidial structures, the absence of septation in conidia, and the presence of milky white watery droplets along with the results of the phylogenetic analysis clearly distinguish YW23-8 from the closest Sarcopodium species. We therefore conclude that strain YW23-8 represents a novel species of the genus Sarcopodium for which we propose the name Sarcopodium terrigenum.

• Journal of Species Research
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• 제11권1호
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• pp.22-28
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• 2022

Two crinoid specimens of genus Tropiometra were collected from Busan and Tongyeong by SCUBA diving on August 2010 and October 2011, respectively. The specimens were identified as Tropiometra macrodiscus (Hara, 1895), which belongs to the family Tropiometridae of superfamily Tropiometroidea. The genus Tropiometra AH Clark, 1907 comprises four species worldwide at present, and it has not been reported in Korea. Tropiometra macrodiscus was first described by Hara (1895) in Japan. It is difficult to distinguish T. macrodiscus from T. afra (Hartlaub, 1890), there has been confusion with examination of their phylogenetic positions in crinoid morphological classification. Despite this, T. macrodiscus can be distinguished from T. afra based on longer arms, stouter whole-body parts, magnificently larger numbers of cirrus, and numerous segments. The morphological characteristics of T. macrodiscus collected in Korea have been described, and DNA barcode region representing cytochrome c oxidase subunit I was obtained for its molecular phylogenetic analysis.

• Mycobiology
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• 제51권4호
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• pp.210-215
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• 2023

The aim of this study was to characterize a new fungal species, Acremonium conglutinatum, isolated from air samples collected in Wando, South Korea. Phylogenetic analysis based on the internal transcribed spacer and large subunit regions revealed its unique position within the genus Acremonium. The isolated strain displayed distinct morphological characteristics, including ellipsoid or bent-ellipsoid conidia formed in clusters on the phialides. These features differentiate the new species from closely related species within the genus. This study describes the morphological and molecular characteristics of A. conglutinatum and emphasizes its phylogenetic relationships with other Acremonium spp. The identification of this novel species contributes to our understanding of the diversity and ecological role of Acremonium.

• 환경생물
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• 제23권4호
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• pp.335-342
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• 2005

We cloned seven genes encoding chitin synthases (CHSs) by PCR amplification from genomic DNAs of four strains of the genus Sporobolomyces and of Bensingtonia subrosea using degenerated primers based on conserved regions of the CHS genes. Though amino acid sequences of these genes were shown similar as 176 to 189 amino acids except SgCHS2, DNA sequences were different in size, which was due to various introns present in seven fragments. Alignment and phylogenetic analysis of their deduced amino acid sequences together with the reported CHS genes of basidiomycetes separated the sequences into classes I, II and III. This analysis also permitted the classification of isolated CHSs; SgCHS1 belongs to class I, BsCHS1, SaCHS1, SgCHS2, SpgCHS1, and SsCHS1 belong to class II, and BsCHS2 belongs to class III. The deduced amino acid sequences involving in class II that were discovered from five strains were also compared with those of other basidiomycetes by CLUSTAL X program. The bootstrap analysis and phylogenetic tree by neighbor-joining method revealed the taxonomic and evolutionary position for four strains of the genus Sporobolomyces and for Bensingtonia subrosea which agreed with the previous classification. The results clearly showed that CHS fragments could be used as a valuable key for the molecular taxonomic and phylogenetic studies of basidiomycetes.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
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• pp.137.2-137
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• 1998

No Abstract, See Full Text

• 한국동물학회:학술대회논문집
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• 한국동물학회 2003년도 한국생물과학협회 학술발표대회
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• pp.129.3-129
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• 2003

No Abstract, See Full Text

• 식물분류학회지
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• 제50권3호
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• pp.253-261
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• 2020

Carex splendentissima U. Kang & J. M. Chung, endemic to the Korean peninsula, is characterized by staminate terminal spikes and glabrous elliptic perigynia. Based on its broad leaves, androgynous spikes, and tri-stigmatic features, the species has been placed in Carex sect. Siderostictae Franch. ex Ohwi, an East Asian section and a basal group in the genus. To clarify the monophyly and phylogenetic position of the species, a molecular study using the internal transcribed spacer region of nuclear ribosomal DNA and chloroplast DNA (trnL-F) data was conducted. The DNA sequence data of ten taxa in sect. Siderostictae and closely related taxa (two taxa in sect. Surculosae) with outgroups were analyzed based on maximum parsimony and maximum likelihood (ML) criteria. In the analyses, C. splendentissima was monophyletic and placed within the Siderostictae clade (sect. Siderostictae + two species of sect. Surculosae), forming a clade with C. ciliatomarginata and C. pachygyna (endemic to Japan). The clade (C. splendentissima + C. ciliatomarginata + C. pachygyna) shows evidence of diploidy. Furthermore, C. splendentissima is a sister to C. ciliatomarginata in the ML tree, and the two taxa have staminate terminal spikes. This study also updates the distribution of C. splendentissima and provides keys to the four Korean taxa in sect. Siderostictae. To conserve the endemic species C. splendentissima, further research on its genetic and ecological features should be conducted at the population level.