• Journal of Crop Science and Biotechnology
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• 제10권1호
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• pp.27-32
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• 2007

RAPD, Inter-SSR, and AFLP markers were used to assess the genetic diversity of lettuce cultivars and the phylogenetic relationships in Lactuca spp. A total of 216 polymorphic bands from seven RAPD primers, four Inter-SSR primers, and five AFLP primer combinations were used to elucidate the genetic similarity among lettuce cultivars. Forty-four lettuce accessions were subdivided into discrete branches according to plant type: crisphead, butterhead, and stem type, with some exceptions. The leafy- and cos-type accessions were intermingled in other groups with no discrete branch indicating that these are more diverse than others. Three accessions, including the Korean cultivar 'Cheongchima', the Korean local landrace 'Jinjam', and the German cultivar 'Lolla Rossa' were classified as the most diverse accessions. Twenty bands were unique in specific cultivars. Among these, three were specific in a plant type; one in Korean leafy type, one in crisphead type, and one in cos type lettuce. In the phylogenetic analysis among Lactuca species, L. saligna, L. serriola, and L. georgica clustered in a sister branch of the L. sativa complex. Two L. virosa accessions show the highest intra-specific relationships. L. perennis outlied from all the other Lactuca species at a genetic similarity of 0.53 and clustered with two Cichorium species, C. intybus and C. endivia, with genetic similarity of 0.67. The phylogenetic tree was supported by data from polymorphism of chloroplast genome which was revealed by PCR-RFLP.

• Journal of Plant Biotechnology
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• 제38권3호
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• pp.234-240
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• 2011

The genus Paeonia belongs to the family Paeoniaceae having significant medicinal and ornamental importance. The present investigation was undertaken with an aim to understand phylogenetic relationships of three Paeonia species (P. lactiflora, P. obovata, and P. suffruticosa) that are widely distributed in China, Korea, and Japan, using nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) sequence and to compare the phylogeny results with investigations reported earlier using existed sequences of the same species. The size variation obtained among sequenced nrDNA ITS region was narrow and ranged from 722 to 726 bp. The highest interspecific genetic distance (GD) was found between P. lactiflora and P. suffruticosa or P. obovata. The phylogram obtained using our nrDNA ITS sequences showed non-congruence with previous hypothesis of the phylogeny between section Paeonia and section Moutan of genus Paeonia. This result was supported by the phylogenetic relations showed in the phylogram constructed with existed sequences in NCBI. The present study suggested that P. obovata belonging to section Paeonia was phylogenetically closer to P. suffruticosa representing section Moutan of genus Paeonia than P. lactiflora belonging to section Paeonia. The main reason of the paraphyly of section Paeonia is thought to be nucleotide additivity directly caused by origin hybridization. This study provides more sequence sources of genus Paeonia, and will help for further studies in intraspecies population, and their phylogentic analysis and molecular evolution.

• 한국균학회지
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• 제47권2호
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• pp.95-104
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• 2019

The genus Ochrolechia is a widespread, lichen genus in Korea. Despite being common, little is known about the species diversity and geographical distribution of Ochrolechia. In this study, we detailed the identification procedure of the genus Ochrolechia in a Korean collection and provided the description of each species. Using 104 specimens collected from 2003 to 2017, we identified four species of the genus Ochrolechia via morphological and/or molecular phylogenetic analysis: O. parellula, O. trochophora, O. yasudae and O. akagiensis. Among them, O. akagiensis had not been previously reported in Korea. Moreover, the species identified as O. frigida and O. tartarea in past studies were corrected as O. yasudae and O. parellula, respectively, based on morphological and/or molecular evidence. Phylogenetic analysis using the internal transcribed spacer regions including 5.8S rRNA gene showed that the four species separated clearly, indicating that the morphological identification corresponds to the phylogenetic identification. We provide a taxonomic key for the four species of the genus Ochrolechia.

• 식물분류학회지
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• 제51권4호
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• pp.345-352
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• 2021

Diarthron linifolium Turcz. is an annual herb usually found in sandy soil or limestone areas. Plants in the genus Diarthron are known to have toxic chemicals that may, however, be potentially useful as an anticancer treatment. Diarthron linifolium is a unique species among the species of the genus distributed in Korea. Here, we determine the genetic variation of D. linifolium collected in Korea with a full chloroplast genome and investigate its evolutionary status by means of a phylogenetic analysis. The chloroplast genome of Korean D. linifolium has a total length of 172,644 bp with four subregions; 86,158 bp of large single copy and 2,858 bp of small single copy (SSC) regions are separated by 41,814 bp of inverted repeat (IR) regions. We found that the SSC region of D. linifolium is considerably short but that IRs are relatively long in comparison with other chloroplast genomes. Various simple sequence repeats were identified, and our nucleotide diversity analysis suggested potential marker regions near ndhF. The phylogenetic analysis indicated that D. linifolium from Korea is a sister to the group of Daphne species.

• 한국미생물·생명공학회지
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• 제39권1호
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• pp.20-28
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• 2011

두 개의 cellulase 유전자 xynB1, bglA2과 groEL1 유전자 염기서열에 기초하여 국내에서 분리한 34균주의 Sorangium cellulosum 균주들을 계통 분석한 결과, 점액세균 중 가장 많은 생리활성물질이 발견된 종인 S. cellulosum 내에는 최소한 5그룹의 소그룹이 존재함을 보였다. 이 분석은 또한 S. cellulosum 균주들의 다양성을 보여주어 분석한 34균주 중 30균주가 서로 다른 균주인 것으로 나타났다.

• Animal Systematics, Evolution and Diversity
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• 제30권2호
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• pp.87-94
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• 2014

Specimens of the genus Orobdella Oka, 1895 from Korea, including various locations in the Korean Peninsula, were identified as Orobdella tsushimensis Nakano, 2011. Phylogenetic analyses using mitochondrial cytochrome oxidase subunit 1 (COI), ND1, $tRNA^{Cys}$, $tRNA^{Met}$, 12S rRNA, $tRNA^{val}$, and 16S rRNA markers show that the newly collected specimens form a monophyletic group with the known O. tsushimensis specimens. The genetic distance of COI of these specimens was in the range 0.4-6.6%. These results confirm that the newly collected specimens belong to O. tsushimensis. This is the first record of the genus Orobdella from the Korean Peninsula.

• International Journal of Industrial Entomology and Biomaterials
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• 제10권2호
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• pp.79-86
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• 2005

Molecular markers have increasingly been used in plant genetic analysis, due to their obvious advantages over conventional phenotypic markers, as they are highly polymorphic, more in number, stable across different developmental stages, neutral to selection and least influenced by environmental factors. Among the PCR based marker techniques, ISSR is one of the simplest and widely used techniques, which involves amplification of DNA segment present at an amplifiable distance in between two identical microsatellite repeat regions oriented in opposite direction. Though ISSR markers are dominant like RAPD, they are more stable and reproducible. Because of these properties ISSR markers have recently been found using extensively for finger printing, pohylogenetic analysis, population structure analysis, varietal/line identification, genetic mapping, marker-assisted selection, etc. In mulberry (Morus spp.), ISSR markers were used for analyzing phylogenetic relationship among cultivated varieties, between tropical and temperate mulberry, for solving the vexed problem of identifying taxonomic positions of genotypes, for identifying markers associated with leaf yield attributing characters. As ISSR markers are one of the cheapest and easiest marker systems with high efficiency in generating polymorphism among closely related varieties, they would play a major role in mulberry genome analysis in the future.

• Journal of Microbiology and Biotechnology
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• 제17권6호
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• pp.1041-1044
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• 2007

To assess the effects of a proton beam on oyster mushrooms (Pleurotus ostreatus), the genetic diversity and phylogenetic relationships among strains induced by a proton beam were investigated based on a clustering analysis. According to an AFLP DNA polymorphism analysis, the induced strains were divided into four groups that coincided with the dose. When applying proton-beam radiation, the dissimilarity among the induced strains increased when increasing the dose. When using more than 400 Gy, the genetic dissimilarity of the irradiated strains was 46-58%. Thus, evaluating the induced strains using the AFLP technique was effective in revealing the mutation effect of the proton beam.

• ALGAE
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• 제31권4호
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• pp.341-349
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• 2016

The red algal genus Amalthea was first described from New Zealand with one species, A. freemaniae. We discovered a new species of Amalthea from Korea, Amalthea rubida sp. nov., and described it based on morphology and rbcL sequences. A. rubida is characterized by a foliose, membranous and soft thallus having loosely arranged anticlinal medullary filaments, much like A. freemaniae, except for a difference of the cortex thickness in the basal part of vegetative structure. In the rbcL phylogenetic analyses, A. rubida formed a clade with A. freemaniae from New Zealand and Halymenia abyssicola from Mexico. A. rubida showed 5.7% interspecific divergence to A. freemaniae and 4.7% to H. abyssicola. Our discovery of A. rubida expands the distribution of Amalthea from the oceanic southwestern Pacific into the continental northwestern. A targeted global study is needed to fully reveal the species diversity and phylogenetic relationships of the genus Amalthea and members of the order Halymeniales.

• Animal Systematics, Evolution and Diversity
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• 제31권4호
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• pp.311-315
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• 2015

Euryalid specimens were collected from Gonghyeonjin and Daejin, Gangwon-do in the East Sea, Korea at a depth of 250-300 m by fishing nets on November 2013 and August 2014. They were identified as Gorgonocephalus arcticus Leach, 1819 belonging to family Gorgonocephalidae of order Euryalida, which was new to the Korean fauna. Nucleotide sequences of partial mitochondrial cytochrome c oxidase I (mt-COI) gene, which was 569 bp in length, were compared among four Gorgonocephalus species, and were subsequently employed to reconstruct phylogenetic trees using the MP, ML, and BI methods. As a result, no sequence difference was found between the G. arcticus mt-COI gene sequences from Korea and Canada, and the two made a strong monophyletic group. With the newly recorded G. arcticus in Korea, in total, four Gorgonocephalus species have been reported in Korea.