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http://dx.doi.org/10.5635/ASED.2014.30.2.087

First Record of Orobdella tsushimensis (Hirudinida: Arhynchobdellida: Gastrostomobdellidae) from the Korean Peninsula and Molecular Phylogenetic Relationships of the Specimens  

Nakano, Takafumi (Department of Zoology, Graduate School of Science, Kyoto University)
Seo, Hong-Yul (National Institute of Biological Resources)
Abstract
Specimens of the genus Orobdella Oka, 1895 from Korea, including various locations in the Korean Peninsula, were identified as Orobdella tsushimensis Nakano, 2011. Phylogenetic analyses using mitochondrial cytochrome oxidase subunit 1 (COI), ND1, $tRNA^{Cys}$, $tRNA^{Met}$, 12S rRNA, $tRNA^{val}$, and 16S rRNA markers show that the newly collected specimens form a monophyletic group with the known O. tsushimensis specimens. The genetic distance of COI of these specimens was in the range 0.4-6.6%. These results confirm that the newly collected specimens belong to O. tsushimensis. This is the first record of the genus Orobdella from the Korean Peninsula.
Keywords
Hirudinida; Gastrostomobdellidae; Orobdella tsushimensis; molecular phylogeny; first record; Korean Peninsula;
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