• 한국산학기술학회논문지
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• 제5권5호
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• pp.484-489
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• 2004

S. platensis로부터 phycobiliprotein을 $30-60{\%}$ 황산암모늄 분별침전법과 Sephadex G-100 gel filtration 및 DEAE-Sephacel anion exchange chromatography의 순서로 4.23의 정제도로 분리하였다. 분리된 phycobiliprotein은 최대흡수 파장이 620 nm인 c-phycocyanin이었다. 분리된 phycobiliprotein은 SDS-PAGE상에서 $({\alpha}$와 $({\beta}$의 두개의 소단위체로 구성되어 있었으며. $({\alpha}$와 $({\beta}$ 소단위체의 분자량은 각각 14.5 kDa과 16 kDa 부근이었다. 또한 gel filtration을 사용한 변성 전 분자량은 약 100 kDa이었다. 이러한 결과는 본 연구에서 분리한 phycobiliprotein이 $({\alpha}{\beta})_{3}-trimer$로 이루어졌음을 보여준다.

• Fisheries and Aquatic Sciences
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• 제25권5호
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• pp.276-286
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• 2022

This study is focused on the effect of phycobiliprotein extraction of Gracilaria on the quality of common carp burgers, and the application of nanoliposomes containing pigment in the improvement of its antimicrobial and antioxidant activity of burgers during refrigerated storage in 18 days. Burgers were incorporated with phycobiliprotein and liposomal phycobiliprotein (2.5% and 5% w/w), and their chemical and microbial changes in terms of pH, peroxide value (PV), thiobarbituric acid (TBA), total volatile basic nitrogen (TVB-N), total viable counts (TVC), psychrotrophic bacterial counts (PTC), and sensory characteristics were evaluated. Results presented a nanoliposome size of about 515.5 nm with capable encapsulation efficiency (83.98%). Our results showed non-encapsulated phycobiliprotein could delay the deterioration of common carp burgers, as a reduction in PV, TBA, and TVB-N, TVC, and PTC values in burgers treated with free and nano encapsulated phycobiliprotein. Moreover, the potential of phycobiliprotein was improved when it was encapsulated into chitosan coated liposomes. Burgers treated with 5% nanoliposomes displayed the lowest amount of lipid oxidation and microbial deterioration in comparison to others during storage. According to chemical, microbial and sensory evaluation, the shelf life of common carp burgers was increased in samples treated with encapsulated phycobiliprotein at 2.5% and 5%, as compared to the control (p ≤ 0.05).

• Journal of Microbiology and Biotechnology
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• 제32권10호
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• pp.1325-1334
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• 2022

Global warming has accelerated in recent decades due to the continuous consumption of petroleum-based fuels. Cyanobacteria-derived biofuels are a promising carbon-neutral alternative to fossil fuels that may help achieve a cleaner environment. Here, we propose an effective strategy based on the large-scale cultivation of a newly isolated cyanobacterial strain to produce phycobiliprotein and biodiesel, thus demonstrating the potential commercial applicability of the isolated microalgal strain. A native cyanobacterium was isolated from Goryeong, Korea, and identified as Pseudanabaena mucicola GO0704 through 16s RNA analysis. The potential exploitation of P. mucicola GO0704 was explored by analyzing several parameters for mixotrophic culture, and optimal growth was achieved through the addition of sodium acetate (1 g/l) to the BG-11 medium. Next, the cultures were scaled up to a stirred-tank bioreactor in mixotrophic conditions to maximize the productivity of biomass and metabolites. The biomass, phycobiliprotein, and fatty acids concentrations in sodium acetate-treated cells were enhanced, and the highest biodiesel productivity (8.1 mg/l/d) was achieved at 96 h. Finally, the properties of the fuel derived from P. mucicola GO0704 were estimated with converted biodiesels according to the composition of fatty acids. Most of the characteristics of the final product, except for the cloud point, were compliant with international biodiesel standards [ASTM 6761 (US) and EN 14214 (Europe)].

• Bulletin of the Korean Chemical Society
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• 제28권10호
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• pp.1639-1640
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• 2007

• 한국미생물·생명공학회지
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• 제52권2호
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• pp.114-121
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• 2024

The present communication deals with the standardization of suitable medium formulation along with anaerobically digested cow's urine (ADCU) for growth of Spirulina subsalsa. Growth was evaluated on the basis of photosynthetic and non-photosynthetic pigment. The results obtained from the study indicated that, SSM-1 and SSM-2 media are suitable for maximum synthesis of chlorophyll-α and carotenoids. The obtained results also indicated that SSM-5 medium is suitable for maximum synthesis of accessory light harvesting pigments phycobiliprotein, total carbohydrate, total protein and total lipid in S. subsalsa. From the study it could be concluded that all the five media combinations (viz. SSM-1, SSM-2, SSM-3, SSM-4 and SSM-5) would be suitable for mass cultivation of S. subsalsa. But among them, SSM-5 medium combination could be the most suitable medium.

• ALGAE
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• 제28권1호
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• pp.121-129
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• 2013

Intact phycobilisomes from a wild-type red Chondrus crispus and its vegetatively derived green mutant were isolated by centrifugation through a discontinuous sucrose density gradient. Pigment composition was subsequently characterized by spectrophotometry. Vegetative thalli of the two strains grown together for six months in the laboratory resulted in different pigment profiles. Two pigmented phycobilisome bands appeared in the sucrose gradient of the wild-type alga, a purple coloured one, and a pink one, whereas only a single blue band appeared in the gradient of the green mutant. Spectrophotometric and fluorescence analyses identified the phycobiliprotein composition of the purple band as the typical phycoerythrin-phycocyanin-allophycocyanin complement in the wild-type, but there was no detectable phycoerythrin present in the blue band of the green mutant. Sodium dodecyl sulphate, preparative polyacrylamide gel electrophoresis analysis confirmed the presence of allophycocyanin subunits in all extracts, but firm evidence of an R-phycoerythrin linker polypeptide in the blue band was missing. These results highlight the ability of C. crispus to adapt to a phycoerythrin deficiency by adjusting light harvesting pigment ratios.

• The Korean Journal of Ecology
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• 제14권1호
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• pp.87-99
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• 1991

Changes of phycobiliproteins(PBP) quantity and ferredoxin-NADP reductase(FNR) activity were investigated in various light illuminated cyanobacteria, Anabaena variabilis. PBP components were increased under blue light illumination, whereas decreased under red light illumination. PBP contents were twofolds in blue light than in red light. In view of the PBP composition, allophycocyanin(APC) in red light was higher 5.5% and phycoerythrocyanin(PEC) in blue light was higher 2.2% than in white light-illuminated PBP. It was suggested that PBP changes in bule light be the results of regulation of photosysthetic efficiency and protection of photosystem, whereas PBP changes in red light be effected by adaptation of adequate harvesting of light energy in photosystem. Changes of FNR activity were highest in red light, and sequenced lower to blue light and green light. It means that light-dependent production rate of NADP is the highest in red light. The difference of values was larger than that of values in comparison of red and blue light. It was suggested that increasing of FNR activity be due not to the function of isozyme, but to the synthesis of enzymes. Because of NAD/NADP regulation-effect to metabolism, it was considered that FNR activity might influence the metabolism indirectly and explain the probability of regulation in pathways of key enzyme activation. FNR activity was directly proportional to intensity of light. Optimum temperature and pH were about 25℃ and 7.5, respectively.

• 한국수산과학회지
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• 제24권6호
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• pp.397-404
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• 1991

남조류와 crytomonads를 포함한 식물플랑크톤의 일차적 형광특성을 in vivo 상태에서 조사하였다. 형광과 exciatation 스펙트럼을 측정한 견과, 약 580-640 nm 영역에 걸친 형광 스펙트럼이, 조사된 모든 식물플랑크톤으로부터 얻어졌다. 관찰된 형광 스펙트럼의 $\lambda_{max}$ 값은, Synechofoccus sp.(SYN)을 제외하고 모두 유사한 것으로 나타났다 $(\pm3nm)$. 관찰된 형광 스펙트럼의 형광소(fluorophore)를 밝히기 위하여, 식물플랑크톤의 형광 스펙트럼의 세기와 모양, $\lambda_{max}$를 식물플랑크톤에 존재하는 유기화합물의 표준용액의 것과 비교하였다. 식물플랑크톤의 fluorescence $lifetime(\lambda)$과 fluorescence decay curve를 식물플랑크톤에 존재하는 유기화합물의 표준용액의 $\lambda$값과 비교하였다. 580 nm의 형광 파장을 사용하여 얻은 식물플랑크톤의 fluorescence decay는 menoex-ponential과 biexponential decay를 보였으며, 식물플랑크톤의 $\lambda(1.39-1.95ns)$ b-phycoerythrin의 표준용액의 $\lambda(3.23ns)$값보다 현저히 작게 나타났다. 이는 이미 알려진 광합성 능력을 가진 intact cell 내부에서 엽록소 a와 biliprotein간의 효율적인 에너지 전달 체계에 의한 $\lambda$값의 단축으로 여겨지며, 580nm의 형광특성이 biliprotein에 의한 것임을 보여준다.