• Bulletin of the Korean Chemical Society
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• v.19 no.2
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• pp.152-154
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• 1998

• Journal of Photoscience
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• v.10 no.3
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• pp.237-240
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• 2003

The tetradecameric peptide (K47-K60) near the NH$_2$-terminal region of epithelial-cell adhesion molecule (Ep-CAM) was chosen as antigenic site and a polyclonal antibody was generated, which could recognize Ep-CAM from the mouse colon tissue or the colon cancer cell, CT-26, in Western blot analysis. Then, the fluorescein isothiocyanate (FITC), a fluorescence dye, was conjugated with the affinity purified Ep-CAM antibody using thiocyanate and the amino groups of FITC and antibody, respectively. The molar ratio of FITC to antibody was estimated approximately 1.86 to 1.00 by measuring the optical densities at 492 nm and 280 nm. Ep-CAM antibody-FITC conjugate was then used for immunohistochemistry of the CT-26 cells. Judging from the shapes formed by fluorescence, the Ep-CAM antibody could delivered FITC to the surface of cells in which Ep-CAM was expressed. This result implies that Ep-CAM antibody could be also used for the tissue-specific delivery of the photosensitizer to the target protein via antigen-antibody interaction.

• Journal of Biomedical Engineering Research
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• v.30 no.2
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• pp.113-121
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• 2009

This study aims at designing and evaluating light source devices that can stably generate light with various wavelengths in order to make possible PDD using a photosensitizer and diagnosis using auto-fluorescence. The light source was a Xenon lamp and filter wheel, composed of an optical output control through Iris and filters with several wavelength bands. It also makes the inducement of auto-fluorescence possible because it is designed to generate a wavelength band of 380-420nm, 430-480nm, and 480-560nm. The transmission part of the light source was developed to enhance the efficiency of light transmission. To evaluate this light source, the characteristics of light output and wavelength band were verified. To validate the capability of this device as PDD, the detection of auto-fluorescence using mouse models was performed.

• Proceedings of the KIEE Conference
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• 2005.05a
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• pp.36-38
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• 2005

Photodynamic diagnosis is a modern method for the fluorescence imaging of cancer. 5-ALA induced protoporphyrin IX fluorescence benefits the tumour selective accumulation of protoporphyrin ; therefore, tumours can be differentiated from healthy tissue. This paper develops Photodynamic diagnosis (PDD) system about ALA that apply tissue absorption coefficient. About other photosensitizer, application capacitate. In this paper, we will expect effective result by working PDD with PDT (photodynamic therapy) system that is a therapy device of cancer.

• Journal of Technologic Dentistry
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• v.23 no.2
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• pp.49-59
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• 2002

The photopolymerization efficiency and surface hardness of composite resin containing 1,2-phenylpropanedione (PD) and diacetyl (DA) as photoinitiators were studied by IR and Vickers hardness and the results were compared with that of camphorquinone (CQ). Relative photopolymerization efficiency of the photoinitiators increased in the order of DA < CQ < PD. Vickers hardness of composite resin containing the photoinitiators increased in the order of CQ < PD < DA. Thus, PD is a new visible light photoinitiator for dental composite resin with higher photopolymerization efficiency and surface hardness than that of CQ. Mechanical properties such as Vickers hardness, diametral tensile strength, and flexural strength of the experimental resin composite prepared by addition of the photosensitizer into a resin of bis-GMA improved with increasing the photosensitizer content and irradiation time. The resin composite of bis-GMA containing DA or PD shows better mechanical properties than that of CQ.

• Rapid Communication in Photoscience
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• v.4 no.2
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• pp.41-44
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• 2015

Biomolecular photo-damaging activity of a water-soluble cationic porphyrin was examined using human serum albumin (HSA), a water-soluble protein as a target biomolecule model by a fluorometry. Dichlorophosphorus(V) tetraphenylporphyrin ($Cl_2P(V)TPP$), was synthesized and used as a photosensitizer. This porphyrin could bind to HSA and cause the photosensitized oxidation of HSA through the singlet oxygen generation and the oxidative photo-induced electron transfer (ET). Near infrared emission spectroscopy demonstrated the photosensitized singlet oxygen generation by this porphyrin. Decrement of the fluorescence lifetime of $Cl_2P(V)TPP$ by HSA supported the ET mechanism. Furthermore, the estimated Gibb's energy indicated that the ET mechanism is possible in the terms of energy. Because oxygen concentration in cancer cell is relatively low, ET mechanism is considered to be advantageous for photosensitizer of photodynamic therapy.

• Archives of Pharmacal Research
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• v.29 no.3
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• pp.188-190
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• 2006

In this study, a newly-synthesized metalloporphyrin, Gd-chlorin (PB Chlorin), was investigated by using a simple tissue phantom to test its efficacy as an MRI contrast agent. This study demonstrated the potential activity of Gd-chlorin as not only a MRI contrast agent, but also as a PDT photosensitizer by using a simple tissue phantom and conducting a very brief MRI experiment.

• Journal of Ginseng Research
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• v.14 no.1
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• pp.30-35
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• 1990

The photooxidation of human erythrocyte ghosts has been studied and the effects of ginseng water extract on it have been investigated. In the presence of photosensitizer, rose bengal, human erythrocyte ghosts have caused photooxidation by lO2 and produced lip)id hydroperoxides. Ginseng water extract and d-${\alpha}$-tocofherol have inhibited photooxidation, ,whereas ascorbic acid has developed in low concentrations but inhibited in high concentrations. On the other hand, amounts of lipid hydroperoxide produced by photooxidation were decreased by addition of catalase after irradiation and according to sequential addition of ginseng water extract into it, the formation of lipid hydrolleroxide was decreased simultaneously.

• Proceedings of the Materials Research Society of Korea Conference
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• 2003.03a
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• pp.229-229
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• 2003

• 한국신재생에너지학회:학술대회논문집
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• 2007.06a
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• pp.238-238
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• 2007