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http://dx.doi.org/10.5857/RCP.2015.4.2.41

Photosensitized oxidative damage of human serum albumin by water-soluble dichlorophosphorus(V) tetraphenylporphyrin  

Ouyang, Dongyan (Department of Optoelectronics and Nanostructure Science, Graduate School of Science and Technology, Shizuoka University)
Hirakawa, Kazutaka (Department of Optoelectronics and Nanostructure Science, Graduate School of Science and Technology, Shizuoka University)
Publication Information
Rapid Communication in Photoscience / v.4, no.2, 2015 , pp. 41-44 More about this Journal
Abstract
Biomolecular photo-damaging activity of a water-soluble cationic porphyrin was examined using human serum albumin (HSA), a water-soluble protein as a target biomolecule model by a fluorometry. Dichlorophosphorus(V) tetraphenylporphyrin ($Cl_2P(V)TPP$), was synthesized and used as a photosensitizer. This porphyrin could bind to HSA and cause the photosensitized oxidation of HSA through the singlet oxygen generation and the oxidative photo-induced electron transfer (ET). Near infrared emission spectroscopy demonstrated the photosensitized singlet oxygen generation by this porphyrin. Decrement of the fluorescence lifetime of $Cl_2P(V)TPP$ by HSA supported the ET mechanism. Furthermore, the estimated Gibb's energy indicated that the ET mechanism is possible in the terms of energy. Because oxygen concentration in cancer cell is relatively low, ET mechanism is considered to be advantageous for photosensitizer of photodynamic therapy.
Keywords
Porphyrin; Photosensitized protein damage; Singlet oxygen; Electron transfer; Redox potential; Binding ability;
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1 Dolmans, D. E. J. G. J.; Fukumura, D.; Jain, R. K. Nat. Rev. Cancer 2003, 3, 380-387.   DOI   ScienceOn
2 Castano, A. P.; Mroz, P.; Hamblin, M. R. Nat. Rev. Cancer 2006, 6, 535-545.   DOI   ScienceOn
3 Hockel, M.; Vaupel, P. J. Natl. Cancer Inst. 2001, 93, 66-76.   DOI   ScienceOn
4 Zhang, S.; Hosaka, M.; Yoshihara, T.; Negishi, K.; Iida, Y.; Tobita, S.; Takeuchi, T. Cancer Res 2010, 70, 4490-4498.   DOI
5 Hirakawa, K. DNA damage through photo-induced electron transfer and photosensitized generation of reactive oxygen species. In: New Research on DNA Damage; Nova Science Publishers Inc.: New York, 2008, pp. 197-219.
6 Hirakawa, K.; Segawa, H. J. Photochem. Photobiol. A: Chem. 1999, 123, 67-76.   DOI
7 Hirakawa, K.; Segawa, H. Photochem. Photobiol. Sci. 2010, 9, 704-709.   DOI   ScienceOn
8 Hirakawa, K.;Fukunaga, N.; Nishimura, Y.; Arai T.; Okazaki, S. Bioorg. Med. Chem. Lett. 2013, 23, 2704-2707.   DOI   ScienceOn
9 Hirakawa, K.; Umemoto, H.; Kikuchi, R.; Yamaguchi, H.; Nishimura, Y.; Arai, T.; Okazaki, S.; Segawa, H. Chem. Res. Toxicol. 2015, 28, 262-267.   DOI
10 Hirakawa, K.; Hirano, T. Nishimura, Y.; Arai, T.; Nosaka, Y. J. Phys. Chem. B 2012, 116, 3037-3044.   DOI   ScienceOn
11 Usui, Y.; Kamogawa, K. Photochem. Photobiol. 1974, 19, 245-247.   DOI
12 Ogilby, P. R.; Foote, C. S. J. Am. Chem. Soc. 1983, 105, 3423-3430.   DOI
13 Sima, J. Coord. Chem. Rev. 2006, 250, 2325-2334.   DOI   ScienceOn
14 Sulkowska. A.; Rownicka. J.; Bojko. B.; Sulkowski, W. J. Mol. Struct. 2003, 651-653, 133-140.   DOI   ScienceOn
15 Hirakawa, K.; Azumi, K.; Nishimura, Y.; Arai, T.; Nosaka, Y.; Okazaki, S. J. Porphyrins Phthalocyanines 2013, 17, 56-62.   DOI