• Title/Summary/Keyword: PhoA

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A Study on the Divinity Construction of Temples in Sapa Region, Vietnam : Case of Den Hang Pho (베트남 사파(Sapa)지역 신전(神殿)의 신격(神格) 구성 고찰 - 덴항포(Den Hang Pho, SAPA)를 소재삼아 -)

  • Lee, Yoon Sun;LE, THI NGOC CAM
    • Journal of Korean Historical Folklife
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    • no.34
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    • pp.253-281
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    • 2010
  • This study has its purpose on figuring it out to the religious notion in Vietnam with the material of the divinity construction of Den Hang Pho in Sapa where is the northern region of Vietnam. To make this, the study divides the type of Vietnamese temples into four parts; Chua, Den, Dinh, and Mieu. Among them, the study pays attention to 'Den' because Vietnamese historical heroes are seated as divinity. This reason makes a reasonable case to this study to figure out a typically and generally religious faith. First of all, the study analyzes immaculate divinity, which generally consists of three and four layered system. The study confirms that the immaculate divinity started from the goddess and then extended to the concept to dominate the sky, the ground, the sea(river), and the mountain(forest). General Tran Hung Dao is the best historical hero in Vietnam and has been placed in temples called Den. The study exams the context that the divinity extended to the religious beliefs, for examples, the belief related in a childbirth by lots of narrations and ceremonies and the belief to treat a sickness, and also exams the context that the divinity placed in a divinity to make a symmetrical relation with immaculate faith. The study exams the divinity construction of Den Hang Pho as its authentic case. In this case, the study could verify a case emphasized by mountain Holy Mother among immaculate faiths. Especially, the study can confirm that General Tran Hung Dao was apotheosized as a concept to be symmetrized with immaculate divinity, and futhermore, a couple of snakes was emphasized by their positioning to every room. Tri-system lays stress on the aboriginality(locality) centered on minority races in the northern Vietnam, the national identity of Vietnam, and the ecological condition of rivers flowing the valley of high and steep peak. The confirmed facts could be said to a construction what the religious notion of tri-system makes. The study makes a conclusion that this kind of conversion-oriented religious notion naturally corresponded with region, nation, and ecologically environmental condition, and extended to the Vietnamese faith with polytheistic divinity.

Air Ventilation Evaluation at Nighttime for the Construction of Wind Corridor in Urban Area (도시지역의 바람길 조성을 위한 야간시간대의 공기순환성 평가)

  • Song, Bong-Geun;Park, Kyung-Hun
    • Journal of the Korean Association of Geographic Information Studies
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    • v.16 no.2
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    • pp.16-29
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    • 2013
  • The purpose of this study is to evaluate air ventilation using wind patterns of MetPhoMod program at nighttime focused on Changwon-si, Gyeongsangnam-do. Evaluation indices of air ventilation are wind resistant and retention used by results of each wind speed and diversity. The results are as follows. Vulnerable areas of air ventilation are Bonglim-dong, Bansong-dong, Yongji-dong and so on. In high-rise apartment, commercial area and single residential area of Yongji-dong, Sangnam-dong and Sapa-dong, wind is stagnated by high buildings. Therefore, these areas should construct urban spaces to circulate the wind. And to inflow persistingly the fresh wind generated in a rural area, we think that the construction of wind corridor is suggested by development plan and policy wind corridor.

Methods for rapid identification of a functional single-chain variable fragment using alkaline phosphatase fusion

  • Lee, Kyung-Woo;Hur, Byung-Ung;Song, Suk-Yoon;Choi, Hyo-Jung;Shin, Sang-Hoon;Cha, Sang-Hoon
    • BMB Reports
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    • v.42 no.11
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    • pp.731-736
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    • 2009
  • The generation of functional recombinant antibodies from hybridomas is necessary for antibody engineering. However, this is not easily accomplished due to high levels of aberrant heavy and light chain mRNAs, which require a highly selective technology that has proven complicated and difficult to operate. Herein, we attempt to use an alkaline phosphate (AP)-fused form of single-chain variable fragment (scFv) for the simple identification of a hybridoma-derived, functional recombinant antibody. As a representative example, we cloned the scFv gene from a hybridoma-producing mouse IgG against branched-chain keto acid dehydrogenase complex-E2 (BCKD-E2) into an expression vector containing an in-frame phoA gene. Functional recombinant antibodies were easily identified by conventional enzyme-linked immunosorbent assay (ELISA) by employing scFv-AP fusion protein, which also readily serves as a valuable immuno-detective reagent.

A study on the Women's Veil of Yi Dynasty (조선조여인의 쓰게에 관한 연구)

  • 정광희
    • Journal of the Korean Home Economics Association
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    • v.20 no.4
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    • pp.13-22
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    • 1982
  • While the women of the Korea Dynasty wore hat(笠子) and Mongsu(蒙首) on top of it when going out, the Yi Dynasty, due to more strict Contucian ethics and isolated way of life, had seen the development of the covering clothes, rather veil, for avoiding the opposite sex with diversity and both men and women began wearing PHO(coat), a Korean topcoat over the head and thurs called Changots, but originally PHO for men was named Changyi a men's outer coat. Neuwul(羅兀), like Mongsu(蒙首) of Korea Dynasty was a thin black hood worn on top of the Wowllip or a kind of skirt draped over the head and the latter was called Changots, very similar to Sgaechima, a kind of long hood formally worn by women. Meanwhile, local women wore a long veil with no arm sleeves dubbed Cheunyi(薦衣) But since western civilization and culture landed on Korea around the end of the 19th century, the women's social status has been raised to almost the same level with men and the way of life has changed towards openness, in which men and women have almost daily contact with one another, so these days we can hardly find such veil style covering clothes around us.

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Effects of Environmental Conditions on Expression of Bacillus subtilis $\alpha$-Amylase in Recombinant Escherichia coli

  • Shin, Pyong-K.;Nam, Seung-H.
    • Journal of Microbiology and Biotechnology
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    • v.2 no.3
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    • pp.166-173
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    • 1992
  • The expression of Bacillus subtilis $\alpha$-amylase from the phoA-amyE fusion gene in recombinant E. coli was investigated under various environmental conditions. The overexpression of cloned $\alpha$-amylase caused retardations in cell growth and synthesis of alkaline phosphatase (AP) from the chromosomal phoA gene. The change of culture temperature from $37^\circ{C}$ to $30^\circ{C}$ increased the specific activities of both $\alpha$-amylase and $\beta$-lactamase by six and two times, respectively, whereas the AP activity remained unchanged. The experiments with chlorampenicol (a translation inhibitor) suggested the enhancement of $\alpha$-amylase activity at $30^\circ{C}$, and this was partly due to the stability of $\alpha$-amylase itself. The further decrease of the temperature to $25^\circ{C}$ slowed down both the cell growth and cloned-gene expression rate. The $\alpha$-amylase activity showed a maximum at pH of 7.4 while alkaline phosphatase was most effectively produced at pH of 8.3.

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Case Study of Tinea Corporis Treatment Diagnosed by KOH Test (한의과에서 KOH 검사로 진단한 체부백선 치료 증례)

  • Lee, Ma-Eum;Jeong, Mi-Rae;Kwon, Kang;Kim, Min-Hee;Seo, Hyung-Sik
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.34 no.2
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    • pp.86-90
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    • 2021
  • Objectives : The purpose of this study is to report the treatment of the Tinea corporis treated by Korean medicine. Methods : The skin lesion with keratin were scraped and placed on a slide glass, and the KOH solution was dropped and observed under a microscope. After the hyphae was confirmed, a ChoPho-bang was prescribed and applied twice a day. Results : In the KOH test performed again 3 weeks after treatment, no hyphae was found and the skin lesion was completely disappeared. Conclusions : For Tinea Corporis, the KOH test is recommended to distinguish it from other skin diseases, and application of ChoPho-bang is effective for treatment.

DEVELOPMENT OF SYSTEM SOFTWARE FOR ASTRONOMICAL OBSERVATIONS BY CCD PHOTOMETRIC SYSTEM IN ASTRONOMICAL OBSERVATORY OF KYUNG HEE UNIVERSITY (CCD를 이용한 경희대학교 천문대 관측시스템 소프트웨어개발)

  • Jin, Ho;Kim, Gap-Seong
    • Publications of The Korean Astronomical Society
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    • v.9 no.1
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    • pp.101-110
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    • 1994
  • We have investigated intensively an optical telescope with 76cm diameter and CCD camera system in astronomical observatory of Kyung Hee university, in order to maximize instrumental functions of our observational equipments and to construct a more reliable photometric system. And computer softwares AUTO DOME, KH CCD and KH PHO for astronomical image observations and their automatic photometries with high accuracy have been made for observers w use our observational system conveniently and efficiently. Throughout careful examinations of these programs, it has been proved that the observing time by our program is shorter than that by manual operations, so that, fast and accurate observations can be executed with ease. For open cluster NGC 7063 observed with S/N value of 350 or more by KH PHO, we have found the magnitude measurements of 11 object stars would show 0.007 magnitude difference, comparing with magnitude data from IRAF/APPHOT. From automatic photometry of eclipsing binary, AB And observed by our software, total 220 data points with good quality have been acquired during 8 hours and so we could make a better light curve than that obtained from any observational results by domestic photoelectric photometry system.

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RNase P-dependent Cleavage of Polycistronic mRNAs within Their Downstream Coding Regions in Escherichia coli

  • Lee, Jung-Min;Kim, Yool;Hong, Soon-Kang;Lee, Young-Hoon
    • Bulletin of the Korean Chemical Society
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    • v.29 no.6
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    • pp.1137-1140
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    • 2008
  • M1 RNA, the catalytic subunit of Escherichia coli RNase P, is an essential ribozyme that processes the 5' leader sequence of tRNA precursors (ptRNAs). Using KS2003, an E. coli strain generating only low levels of M1 RNA, which showed growth defects, we examined whether M1 RNA is involved in polycistronic mRNA processing or degradation. Microarray analysis of total RNA from KS2003 revealed six polycistronic operon mRNAs (acpP-fabF, cysDNC, flgAMN, lepAB, phoPQ, and puuCBE) showing large differences in expression between the adjacent genes in the same mRNA transcript compared with the KS2001 wild type strain. Model substrates spanning an adjacent pair of genes for each polycistronic mRNA were tested for RNase P cleavage in vitro. Five model RNAs (cysNC, flgMN, lepAB, phoPQ, and puuBE) were cleaved by RNase P holoenzyme but not by M1 RNA alone. However, the cleavages occurred at non-ptRNA-like cleavage sites, with much less efficiency than the cleavage of ptRNA. Since cleavage products generated by RNase P from a polycistronic mRNA can have different in vivo stabilities, our results suggest that RNase P cleavage may lead to differential expression of each cistron.

The Emergence of Early Polities and Communities on the Trans-Peninsular Routes in the Thai-Malay Peninsula

  • Pipad KRAJAEJUN
    • Acta Via Serica
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    • v.9 no.1
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    • pp.33-66
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    • 2024
  • There is a lot of evidence of early port cities and small habitation sites along the Thai-Malay Peninsula's coastlines. These sites appeared on the trans-peninsular routes during the Maritime Silk Roads period, from 2,000 to 1,500 BP. The Thai-Malay Peninsula was mentioned in many documents as Suvarnabhumi (India), Aurea Chersonese (Greek), and Jin Lin (Chinese), meaning the Golden Land. Ptolemy's map displays that there were many port cities along this peninsula. It corresponds to the Milinda-panha, which depicts many port cities in Suvarnabhumi. Foreign documents primarily documented the presence of major port cities, while inland habitation sites received less recognition. This paper aims to reconstruct the trans-peninsular routes in order to understand their networks, connections, and roles. The second aim is to understand the hierarchy of each site. This paper will focus on two important settlements and high-value artifacts along three routes: the Kra Isthmus Route, the Pho Khao Thong and Tha Chana Route, and the Krabi to Surat Thani Route. I conducted an archaeological survey of these three routes and others during 2021-2023 under the Suvarnabhumi Studies Center, TASSHA Institute, and the Ministry of Higher Education, Science, Research, and Innovation.

Construction of a Temperature Controlled Expression Ve e tor in Saccharumy ces cerevisiae (Saccharomyces cerevisiae를 이용한 온도조절형 발현 Vector의 개발)

  • 최진옥;황용일
    • Microbiology and Biotechnology Letters
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    • v.21 no.3
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    • pp.214-220
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    • 1993
  • The mating type a of yeast, Saccharomyces cerevisiae mutant with hmla2-102 and sir3-8ts was changed to type alpha by changing the growth temperature from 25C to 35C. A temperature-sensitive expression vector system was constructed using mating factor alpha1 (Mfalpha1) gene encoding alpha factor which is expressed in the type alpha cells. Vectors with different copy numbers were constructed by joining the promoter and pre or prepro-secretion single sequence of Mfalpha1 to promoterless PHO5' gene as a reporter gene.

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