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Heat Shock Protein Augmentation of Angelica gigas Nakai Root Hot Water Extract on Adipogenic Differentiation in Murine 3T3-L1 Preadipocytes

  • Lumbera, Wenchie Marie L.;Cruz, Joseph dela;Yang, Seung-Hak;Hwang, Seong Gu
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.3
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    • pp.419-427
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    • 2016
  • There is a high association of heat shock on the alteration of energy and lipid metabolism. The alterations associated with thermal stress are composed of gene expression changes and adaptation through biochemical responses. Previous study showed that Angelica gigas Nakai (AGN) root extract promoted adipogenic differentiation in murine 3T3-L1 preadipocytes under the normal temperature condition. However, its effect in heat shocked 3T3-L1 cells has not been established. In this study, we investigated the effect of AGN root hot water extract in the adipogenic differentiation of murine 3T3-L1 preadipocytes following heat shock and its possible mechanism of action. Thermal stress procedure was executed within the same stage of preadipocyte confluence (G0) through incubation at $42^{\circ}C$ for one hour and then allowed to recover at normal incubation temperature of $37^{\circ}C$ for another hour before AGN treatment for both cell viability assay and Oil Red O. Cell viability assay showed that AGN was able to dose dependently (0 to $400{\mu}g/mL$) increase cell proliferation under normal incubation temperature and also was able to prevent cytotoxicity due to heat shock accompanied by cell proliferation. Confluent preadipocytes were subjected into heat shock procedure, recovery and then AGN treatment prior to stimulation with the differentiation solution. Heat shocked preadipocytes exhibited reduced differentiation as supported by decreased amount of lipid accumulation in Oil Red O staining and triglyceride measurement. However, those heat shocked preadipocytes that then were given AGN extract showed a dose dependent increase in lipid accumulation as shown by both evaluation procedures. In line with these results, real-time polymerase chain reaction (RT-PCR) and Western blot analysis showed that AGN increased adipogenic differentiation by upregulating heat shock protection related genes and proteins together with the adipogenic markers. These findings imply the potential of AGN in heat shock amelioration among 3T3-L1 preadipocytes through heat shock factor and proteins augmentation and enhanced adipogenic marker expression.

Some Aspects of High Lysine Maize Breeding using Opaque-2 Gene (Opaque-2 인자를 이용한 고라이신 옥수수의 육종)

  • Bong-Ho Chae
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.5 no.1
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    • pp.57-64
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    • 1969
  • Several field and sweet corn varietiea from several sources were crossed with a variety carrying the opaque-2 gene to determine the phenotypic interactions in the breeding of high lysine maize. Although opaque-2 lines showed lower protein content than the corresponding normal varieties, there was no correlation between the protein levels of the two types. opaque-2 maize contained more lysine, but no relationship was found between the protein content and the lysine content of either normal or opaque-2 types, suggesting that high lysine corn using the opaque-2 gene may be developed independently from the protein content. The F2 segregation ratios for normal and opaque-2, 100-kernel weights, percentage seed set, opaque-2 phenotype, disease susceptibility, and the relationship between protein and lysine content of normal and opaque-2 were investigated. The determinations and observations were made on the F2, F3, and BC1 Lysine content was determined by the ion exchangeresin combined with paper chromatography method. Most crosses segregated in a 1-opaque-2 : 3-normal ratio as expected. Opaque-2 segregates were lighter than the normal type and smaller in size. A mottled phenotype of opaque-2 maize observed in the Philippines yellow endersperm. In some varieties opaque-2 maize was very susceptible to the ear and kernel rot disease. No. 5(female) and opaque-2(male). Selectlon of a double mutant of waxy and opaque-2 by using the iodine technique and electric lamp was discussed. opaque-2 and floruy-2 were not allels. Different percentage of seed set were observed in the segregation of aewx crossed with opaque-2. An unusual gametophytie relationship was involved in a cross between Glutinous.

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A Study on the Effects of Psychological Capital on Pro-social Organizational Behavior in Local Subsidiary Abroad : Focusing on the Type of Leadership as a Moderator. (해외법인 현지국 직원의 긍정심리자본과 친사회적 조직 행동 간의 관계 - 리더십 유형의 조절효과를 중심으로 -)

  • Yoo, SungJoon;Kim, Hag-min
    • International Commerce and Information Review
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    • v.19 no.1
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    • pp.279-304
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    • 2017
  • The objective of this study has two filds. The first is to examine the effect of native employee's psychological capital on their pro-social organizational behavior in the context of a local subsidiary in abroad. The second is to investigate the moderation effect of non-native managers' leadership type in the relationships between psychological capital and pro-social organizational behavior. The researcher provides the theoretical research model based on the pro-social capital theory and then empirically tests the model by using a valid 334-case collected from three overseas-affiliated-companies in Philippines. The results show that native employees' psychological capital positively and significantly influences to pro-social organizational behavior. Moreover, non-native managers' type of leaderships has the significant moderation effects in relationships between native workers' psychological capital and pro-social organizational behavior. Specifically, stronger relationships are identified in all of relationships when the managers show transactional, transformational and authentic leaderships. Theoretical contributions and practical recommendations are also discussed based on the results of the study.

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Studies on Zinc deficiency in paddy soil -I. Zinc deficiency of the rice plant in Korea and Philippine soils (답토양(畓土壤)에서의 아연결핍(亞鉛缺乏)에 관(關)한 연구(硏究) -I. 수도(水稻)에 있어서 아연결핍(亞鉛缺乏)에 관(關)한 한국(韓國)과 비율빈 답토양(畓土壤)에 대(對)한 비교연구)

  • Ahn, Jong Sung
    • Korean Journal of Soil Science and Fertilizer
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    • v.5 no.1
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    • pp.41-47
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    • 1972
  • An experiment was conducted to study the zinc deficiency of rice plant grown on Boeun soil of Korea and on Batangas soil of the philippines. The results obtained are summarized as follows: 1. Batangas soil was deficient in zinc because of the high pH, high organic matter and high calcium. Zinc content of the rice plant grown on Batangas soil was l6ppm. On the other hand, Boeun soil had low organic matter. Zinc content of the rice plant grown on Boeun soil was 33ppm. 2. The top leaf had higher zinc content than any other leaves. Zinc deficiency apparently occured when the zinc content of the rice plant was below 18ppm. 3. The physiologically acid fertilizer such as ammonium sulfate was more advantageous in improving zinc up take than the urea. 4. The zine deficiency was promoted when the Mn/Zn ratio is high, and zinc and manganess interact antagonistically in the growth of rice plant. 5. The zinc deficiency could be overcome by applying zinc chloride and fritted Trace elements to the Batangas soil, and the yield was significantly increased than conrtol.

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Study on Utilization and Prospect of Lignocellulosic Bioethanol in ASEAN Countries (주요 ASEAN 국가의 목질계 바이오에탄올의 활용 및 전망에 관한 연구)

  • Heo, Su Jung;Choi, Joon Weon
    • Journal of the Korean Wood Science and Technology
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    • v.45 no.5
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    • pp.588-598
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    • 2017
  • Currently, bioethanol, a fuel additive for transportation, is produced mainly by using biomass (first generation) such as corn and sugar canes. First generation biomass can cause various problems in terms of increase in agricultural prices and ethical reasons. To address these problems, a nonedible lignocellulosic biomass can be utilized. Agricultural byproducts such as straw, bagasse, and forest byproducts from the wood processing industry. Therefore, production of wood based bioethanol can be an effective utilization route of second generation biomass, and its raw materials are more abundant than first generation resources. Furthermore, it is possible to secure cheap raw materials. One of the biggest advantages of using biofuels is that it contributes to the reduction of greenhouse gases by minimizing the environmental impact, unlike fossil fuels. In this study, we investigated the greenhouse gas reduction effects that can be achieved through the use of Lignocellulosic bioethanol and government policies on renewable energy currently being implemented in ASEAN countries (Indonesia, Malaysia, Thailand and the Philippines). In these four countries, policies and incentives related to biofuels have been developed. It is expected that the reduction ratio of carbon dioxide emission and the mixed biofuel will be gradually increased in the future.

Microbial bioconversion of natural Philippine nut oils into a value-added hydroxy fatty acid, 7,10-dihydroxy-8(E)-octadecenoic acid (미생물 생변환을 통한 필리핀 너트유로부터 기능성 지방산 7,10-dihydroxy-8(E)-octadecenoic acid 생산)

  • Dasangrandhi, Chakradhar;Ellamar, Joel B.;Kim, Young Soon;Kim, In Hwan;Kim, Hak-Ryul
    • Korean Journal of Food Science and Technology
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    • v.49 no.1
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    • pp.28-34
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    • 2017
  • Biocatalytic modification of natural resources can be used to generate novel compounds with specific properties, such as higher viscosity and reactivity. The production of hydroxy fatty acids (HFAs), originally found in low quantities in plants, is a good example of the biocatalytic modification of natural vegetable oils. HFAs show high potential for application in a wide range of industrial products, including resins, waxes, nylons, plastics, lubricants, cosmetics, and additives in coatings and paintings. In a recent study, Pseudomonas aeruginosa strain PR3 was used to produce 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) from oleic acid. This present study focused primarily on the utilization of three natural nut oils obtained from the Philippines -pili nut oil (PNO), palm oil (PO), and virgin coconut oil (VCO)- to produce DOD by P. aeruginosa strain PR3. Strain PR3 produced DOD from PNO and PO only, with PNO being the more efficient substrate. An optimization study to achieve the maximum DOD yield from PNO revealed the optimal incubation time and medium pH to be 48 h and 8.0, respectively. Among the carbon sources tested, fructose was the most efficiently used, with a maximum DOD production of 130 mg/50 mL culture. Urea was the optimal nitrogen source, with a maximum product yield of 165 mg/50 mL culture. The results from this study demonstrated that PNO could be used as an efficient substrate for DOD production by microbial bioconversion.

Statistical Analysis of Maritime Traffic Volume at Manila Bay, Philippines (필리핀 마닐라만의 해양 교통량 통계분석)

  • Dimailig, Orlando S.;Jeong, Jae-Yong
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.18 no.4
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    • pp.323-330
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    • 2012
  • Manila Bay is home to the Port of Manila with three harbors: North Harbor, South Harbor and MICT(Manila International container Terminal). There is an adjacent fishing port to the north and another port across the Bay, the Limao Port. This study focuses on the volume of traffic movement in the Bay area taken from Manila VTMS raw data of the arrival and departure movements only. It is a two-year period of study of 2010 and 2011 traffic volume. It divides the data according to their numbers; to their sizes measured in gross tons; to the time of vessels' movements, whether daytime or night-time; and to each voyage trade: domestic or foreign. Quantitative values are calculated from the raw data based on the whole population of the two-year period. The results are illustrated by tables and graphs. Statistical measures are applied to determine the spread and frequencies of the data and test any significance from the hypotheses. These are shown in the tabulated form and interpreted to give a better picture of the frequency and volume of traffic. In the end, a summary is offered where it is hoped that this paper will propel further studies of improving the safety behavior in the premier port of the country.

Application of Dietary Reference Intakes for Codex Nutrient Reference Values (코덱스 영양소 기준치 설정시 영양섭취기준의 적용 방안)

  • Joung, Hyo-jee;Yoon, Jin-Sook;Choi, Seul-Ki;Shin, Sang-Ah;Choi, Young-Sun;Kwon, O-Ran;Chang, Nam-Soo
    • Journal of Nutrition and Health
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    • v.42 no.4
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    • pp.366-373
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    • 2009
  • Codex Nutrient Reference Values (NRVs) were based on the 1988 Helsinki report and a single set of NRVs was established for the general population over 3 years for the purpose of food labeling in 1993. Dietary Reference Intakes (DRIs) with new concepts were developed in many countries recently. Hence there has been broad discussion regarding establishment of new NRVs using DRIs, which include estimated average requirement (EAR), recommended intake (RI or RDA), adequate intake (AI), and tolerable upper intake level (UL) in the world. This study was carried out to review various possible values for Codex NRVs such as population-weighted EAR, population-weighted RDA, and populationbased RDA. The values were simulated using DRIs and population distribution of Korea, USA, Japan, Philippines, Germany and England, and compared to the current Codex values and the highest RDA and the lowest UL among populations with different life-stage. Since population weighted/based values are necessary to be updated according to the population changes and are different across countries, inconsistency can be a serious barrier in international transactions. For some of nutrients such as vitamin A and zinc, values based on population-based RDA or the highest RDA were higher than the lowest UL. Therefore, careful considerations should be given before establishing Codex NRVs.

An Study on World Geography Lessons Using Local Multi-cultural Activities and the CCAP (Cross-Cultural Awareness programme) (지역 다문화 활동과 CCAP를 활용한 세계지리 수업에 관한 연구)

  • Kim, Si-Gu;Cho, Chul-Ki;Jo, Hyun-Mi
    • Journal of the Korean association of regional geographers
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    • v.17 no.2
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    • pp.231-244
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    • 2011
  • This study is to examine how activities using local multi -cultural resources and world geography lessons using the CCAP(Cross-Cultural Awareness Programme) affect students' multi-cultural understanding. Activities using local multi-cultural resources were carried by visiting in an alien worker community, volunteering in multicultural center, attending on UNESCO ASP(Associated School Program), and making radio broadcasting program associated with 'SCN FM' which is a local broadcasting station on the weekend. And world geography lessons using the CCAP were conducted with 5 classes including orientation by inviting foreign instructors from that place after reconstructing the Southeast and South Asia unit of the countries such as Pakistan, the Philippines, Myanmar and Cambodia. According to twice questionnaire survey conducted before and after activities using local multi-cultural resources and world geography lessons using the CCAP, it appeared that students had familiarity more than before through direct meeting with foreigners inside and outside classroom, and understood and respected other cultures by acquiring contextual regional knowledge. In the end, multi-cultural activities and world geography lessons using the CCAP contributed to students' global citizenship, which overcame prejudice toward third world cultures. Like that, if world geography classes provide continually students with opportunities to experience directly diverse cultures inside and outside classroom, world geography overcome negative image that it transmit decontextual regional knowledge and is regarded as the subject that is very fit for nurturing global citizenship based on contextual knowledge, consideration and tolerance needed in global and multi-cultural society.

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Environmental Control Accomodative to Ecosystem on the Prevention of Nuclear Polyhedrosis Virus Disease in the Silkworm. Bombyx mori (생태친화적(生態親和的) 사육환경(飼育環境) 제어방식(制御方式) 확립(確立)을 위한 누에 핵다각체병(核多角體病) 발생요인(發生要因) 분석(分析))

  • Han, Myung-Sae;Lim, Jong-Sung
    • Current Research on Agriculture and Life Sciences
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    • v.15
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    • pp.69-81
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    • 1997
  • For the prevention of worldwide prevalent disease of nuclear polyhedrosis virus (NPV), environmental conditions and their incidence of grasserie was investigated through 57 cases of silkworm rearing from the year of 1979 to 1993 in the countries of Korea, Japan, and Philippines. Relationship between the occurrence of NPV and environmental factors were also analysed from the aspect of causal pathogenesis. Unfavorable foactors related to the prevalence of NPV disease was reconfirmed by the assay of experimental rearing. Silkworms reared on mulberry leaves or artificial diet appeared similar result on the occurrence of grasserie. Disinfection by formalin and simple sweeping or washing was not significantly different on the occurrence of NPV disease. Following insufficient ventilation on the younger larvae. from the 1st to 3rd instar, the disease by NPV at the later stage was remarkably emphasized those insidence. An experimental rearing from 1993 to 1996 demonstrated the prevention of NPV disease by simple cleaning of sweeping under the condition of air forced ventilation, the customal practice of disinfection with formalin or any other chemical agents could be omissible.

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