• Title/Summary/Keyword: Phenylhydrazine

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Synthesis of 6-Alkyl-3-Chromonealdehyde(2,2-dialkyl)hydrazone Derivatives for Green Light Emitting Materials (녹색발광 6-알킬-3-크로몬알데히드(2,2-디알킬)하이드라존 유도체의 합성)

  • Chung, Pyung-Jin;Chang, Hong-Joon
    • Applied Chemistry for Engineering
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    • v.21 no.4
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    • pp.424-429
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    • 2010
  • 6-Alkyl-3-chromonealdehyde (2,2-dialkyl)hydrazone derivatives were synthesized by dehydration condensation. They are green-emitting materials for organic light emitting device (OLED) composed of electron acceptor of 6-alkyl-3-chromonealdehydes and electron donor of 2,2-dialkylhydrazones in a conjugated structure. The structural properties of reaction products were analyzed by FT-IR and $^1H$-NMR spectroscopy. The thermal stabilities and reactivities were measured by melting points and yields. The UV-visibles and PL properties were also determined by excitation spectra and emission spectra, respectively.

Antioxidant activity of Cinchona officinalis stem bark extracts

  • MN, Ravishankara;Padh, Harish;M., Rajani
    • Advances in Traditional Medicine
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    • v.3 no.4
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    • pp.205-211
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    • 2003
  • Stem bark of Cinchona sp. (Rubiaceae) is one of the well known drugs for its therapeutic values in traditional as well as modern medicine. Even though a lot of work has been carried out on quinoline alkaloids of Cinchona, its phenolic constituents received very little attention. In the present study, we evaluated antioxidant properties of C. officinalis stem bark methanolic extract and water extract containing phenolic compounds (total phenolics 21.37, 5.18% w/w respectively in the two extracts) in different in vitro and ex vivo models viz., antiradical activity by DPPH reduction, superoxide radical scavenging activity in riboflavin/light/NBT system, nitric oxide radical scavenging activity in sodium nitroprusside/Greiss reagent system and inhibition of lipid peroxidation induced by iron-ADP-ascorbate in liver homogenate and haemolysis of erythrocytes induced by phenylhydrazine in erythrocyte membrane stabilization study. Both the extracts exhibited very good antioxidant activity in all the models tested. The phenolic compounds including tannins present in the stem bark seem to offer protection from the oxidative damage.

Synthesis of 3-Chromonealdehyde(2,2-disubstituted)hydrazone Derivatives for Green Light Emitting Materials (녹색발광 3-크로몬알데히드(2,2-이치환)하이드라존 유도체의 합성)

  • Chung, Pyung Jin;Chang, Hong Joon
    • Applied Chemistry for Engineering
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    • v.20 no.6
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    • pp.670-674
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    • 2009
  • 3-Chromonealdehyde(2,2-disubstituted)hydrazone derivatives were synthesized by dehydration condensation. They are green-emitting materials for organic light emitting device (OLED) composed of electron acceptor of 3-chromonealdehydes and electron donor of 2,2-disubstituted hydrazones by a conjugated structure. The structural properties of reaction products were analyzed FT-IR and $^1H-NMR$ spectroscopy. The thermal stabilities and reactivities were measured by melting points and yields. The UV-visibles and PL properties can be determined by excitation spectra and emission spectra, respectively.

Bezoin in Heterocyle Synthesis: Synthesis and Reactions of 2, 3-Diphenyl-4-cyanopyrrole-5-thione

  • Khalifa, Fathy A.;Zohdi, Hussein F.;Ibrahim, M.K.A.;Ismail, N.A.
    • Archives of Pharmacal Research
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    • v.13 no.4
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    • pp.351-354
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    • 1990
  • 2, 3-diphenyl-4-cyano-pyrrole-5-thione (4) was either by the reaction of benzoin (1) and cyanothioacetamide (3) followed by cyclization using AcOH/sodium acetate or by refluxing a mixture of benzoin (1) and cyanothioacethamide in pyridine to afford directly 4. Several new pyrrole and pyrazole derivatives were synthesised using 4 as synthon. The structure of the newly synthesised derivatives were based on celemental and spectral data studies. Methylation of the SH group in 4 afforded 5. Reaction of 4 with ethyl bromo acetate afforded (6). Treatment of (5) and (6) with hydrazine hydrate afforded the same pyrazole derivative (10) through the intermediate (9). Treatment of 6 with aniline and phenylhydrazine afforded the pyrrole derivatives 8a, b respectively. Treatment of 6 while dill HCI gave 2, 3-diphenyl-4-cyano-pyrrole-5-one (7). Treatment of 6 with $NH_3$/EtOH afforded the amidic derivatives (11) with treatment of 6 $NH_3$/ heat then acidification it gave the carboxylic derivatives (12).

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Analytical method for analyzing formaldehyde using 2, 4-DNPH and gas chromatography/FID, NPD (2,4-DNPH와 가스크로마토그래프를 이용한 포름알데히드 분석방법)

  • Jeong, Jee Yeon;Park, Seung Hyun;Yi, Gwang Yong;Oh, Se Min
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.10 no.1
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    • pp.126-146
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    • 2000
  • To develop and evaluate formaldehyde measurement method using 2,4-dinitro-phenylhydrazine (2,4-DNPH) coated sampler and gas chromatography, laboratory test and field test were conducted. Results of this study are as follows. Limit of detection(LOD) of measurement methods, HPLC-UVD, GC-NPD and GC-FID, is $0.008{\mu}g/m{\ell}$ $0.060{\mu}g/m{\ell}$, $0.472{\mu}g/m{\ell}$ respectively. Coefficiency of measurement methods, HPLC-UVD, GC-NPD and GC-FID, is 0.008, 0.009, 0.020 respectively. Desorption efficiency of sep-pak xposure aldehyde sampler and sorbent sample tube is 1.05(range : 0.99 - 1.12), 1.02(range : 0.99 - 1.06) respectively. Samples of sorbent sample tube and sep-pak xposure aldehyde sampler turned out to be stored at refrigerator, according to storage test results. Measurement methods of HPLC-UVD, GC-NPD, GC-FID, according to results of precision for the combined sampling and analytical procedure, became acceptable to OSHA evaluation standard. Field test using exposure chamber met the NIOSH overall uncertainty recommendation(less than 25%). Overall uncertainty of Sepak-HPLC(UVD), Tube-GC(NPD), Tube-GC(FID) is 11.0% - 17.0%. Consequently gas chromatography(GC-NPD, GC-FID) and high performance liquid chromatography(EPA TO-11) using 2,4-DNPH coated sampler for formaldehyde measurement turned out to be suitable to measure personal formaldehyde exposure at workplaces.

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Studies on the Utilization of Phenolic Substance by Yeast (효모에 의한 phenol 성 물질의 자화에 관한 연구)

  • 김상달;서정훈
    • Microbiology and Biotechnology Letters
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    • v.6 no.4
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    • pp.155-159
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    • 1978
  • Phenol utilizing yeast No. 558 isolated from soil sewage sediment was able to use substantial amount of phenol as the sole carbon source, and the biomass productivity by this organism was very excellent. This organism could grow well in 1000 ppm of phenol concentration, the maxim-um specific growth rate obtainable at pH 5.0, 3$0^{\circ}C$ was 0.27/hr., and the biomass yield coefficient Y vs. consumed phenol was 3.2. Maximum production rate of biomass was observed at 35$^{\circ}C$, pH 3.5 to pH 4.5, and the addition of the 0.005~0. 01% yeast extract was the most effective. Addition of HgCl$_2$ and phenyl hydrazine, inhibitors of oxide-reductase, in the phenol containing cultural liquid caused this organism no-growth at the concentration of 10$^{-5}$ M, 10$^{-3}$ M respectively. This organism could utilize not only phenol but catechol, resorcinol and benzidine.

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Characterization of Thermostable Tyrosine Phenol-Lyase from an Obligatory Symbiotic Thermophile, Symbiobacterium sp. SC-1

  • Lee, Seung-Goo;Hong, Seung-Pyo;Kwak, Mi-Sun;Esaki, Nobuyoshi;Sung, Moon-Hee
    • BMB Reports
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    • v.32 no.5
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    • pp.480-485
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    • 1999
  • Tyrosine phenol-lyase of thermophilic Symbiobacterium sp. SC-1, which is obligately and symbiotically dependent on thermophilic Bacillus sp. SK-1, was purified and characterized. The enzyme is composed of four identical subunits and contains approximately 1 mol of pyridoxal 5'-phosphate (PLP) per mol subunit as a cofactor. The enzyme showed absorption maxima at 330 and 420 nm, and lost this absorption profile by treatment with phenylhydrazine. The apparent dissociation constsnt, $K'_D$, for PLP was determined with the apoenzyme to be about $1.2\;{\mu}M$. The isoelectric point was 4.9. The optimal temperature and pH for the $\alpha,\beta$-elimination of L-tyrosine were found to be $80^{\circ}C$ and pH 8.0, respectively. The substrate specificity of the enzyme was very broad: L-amino acids including L-tyrosine, 3,4-dihydroxyphenyl-L-alanine (L-DOPA), L-cysteine, L-serine, S-methyl-L-cysteine, $\beta$-chloro-L-alanine, and S-(o-nitrophenyl)-L-cysteine all served as substrates. D-Tyrosine and D-serine were also decomposed into pyruvic acid and ammonia at rates of 7% and 31% relative to their corresponding L-enantiomers, respectively. D-Alanine, which was inert as a substrate in a, $\beta$-elimination, was the only D-amino acid racemized by the enzyme. The $K_m$ values for L-tyrosine, L-DOPA, S-(o-nitrophenyl)-L-cysteine, $\beta$-chloro-L-alanine, and S-methyl-L-cysteine were 0.19, 9.9, 0.36, 12, and 5.5 mM, respectively.

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Effect of Zona Hardening on In Vitro Fertilization in Mouse Oocytes II. Analysis of Materials Causing Zona Hardening (생쥐난자에 있어서 투명대 경화현상이 체외수정에 미치는 영향 II. 투명대 경화 현상을 유도하는 원인물질의 구명)

  • 이상진;정길생
    • Korean Journal of Animal Reproduction
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    • v.17 no.3
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    • pp.173-181
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    • 1993
  • In order to demonstrate whether ovoperoxidase hardens the zona of oocytes activated by incubating in M-S buffer supplemented with 20$\mu$M of Ca-ionophore A 23187, the effect of peroxidase inhibitors(250mM pheylhydrazine, 28mM sodilum sulfite, 350mM glycine ethyl ester and 50mM sodium azide), tyrosine analogue(12.5mM tyramine) and exogeneous peroxidase(50$\mu\textrm{g}$/ml horseradishperoxidase ; HRP) on zona hardening in ionorphore-treated oocytes were investigated. The results obtained from thses experiments were summarized as follows : 1. The zona solubility (t50) of ionophore-activated and DMSO-treated oocytes at 1, 2 and 3 hr of culture were 25.0, 31.6 and 40.6min., and 9.7, 10.8 and 15.5 min., respectively. The longest time required for zona lysis of ionophore activated oocytes at 1 hr after onset of ionophore treatment. The diferences int50 for zona was significantly greater as compared to DMSO-treated controls(P<0.01). 2. The inhibition rates of hardening in the oocytes treated with the phenylhydrazine, sodium sulfite, glycine ethyl ester and sodium azide, were 23.8, 61.9, 95.2 and 23.8%l, respectively, and the tyramine, was 14.3%. Several known peroxidase inhibitors and tyrosine analogue were blocked zona hardening in ionophore activated oocytes. 3. The treatment of exogeneous peroxidase promoted the zona hardening of activated oocytes but not unactivated oocytes. These resuls indicate that the ovoperoxidase apparently catalyzes the hardening of the zona following ionophore activation of mouse oocytes.

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Searching of Possible Target Enzymes for Herbicide Development using Commercial Plant-Specific Inhibitors (식물 특정효소저해제의 생물활성 조사에 의한 신규제초제 작용점 탐색)

  • Hwan, In-Taek;Choi, Jung-Sup;Park, Sang-Hee;Lee, Kwan-Hwi;Lee, Byung-Hoi;Hong, Kyung-Sik;Cho, Kwang-Yun
    • The Korean Journal of Pesticide Science
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    • v.5 no.1
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    • pp.36-45
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    • 2001
  • This study was conducted to search new target enzymes of novel herbicide candidate. Total of 107 biochemical inhibitors reported to inhibit over than 100 different plant enzymes were purchased from commercial chemical companies. 15 inhibitors and 34 enzymes were selected by germination assay, seedling assay, wheat leaf disc assay, and whole plant assay. Among them, seven compounds of purine, phehyl-hydrazine, o-phenanthroline, oleylamine, dicyclohexylcarbodiimide, 7,8-benzoquinoline, and aminooxyacetic acid showed high herbicidal activity in the whole plant assay under greenhouse while 7,8-benzoquinone, 8-hydroxyquinoline, 2,2'-dipyridyl, and o-phenanthroline inhibited seed germination of barnyardgrass, rice, and tomato at concentrations of 1.25 to $5{\mu}M$. The compounds of 7,8-benzoquinoline, chlorpromazine, cyanuric fluoride, 4-methylpyrazole, oleylamine, tranylcypromine, and trifluoperazine inhibited the growth of cyanobacteria at 30 to $100{\mu}M$. The compounds of dicyclohexylcarbodiimide and chlorpromazine exhibited whitening effect on tile wheat leaf disc at $100{\mu}M$. These results suggest that the plant-specific enzyme inhibitors which have biological activities may supply the target enzyme for developing new herbicide candidate.

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Effects of Combination of Danggwibohyeol-tang Herbal-acupuncture on Anemic Rats (배합비율(配合比率)에 따른 당귀보혈탕(當歸補血湯) 약침(藥鍼)이 빈혈백서(貧血白鼠)에 미치는 영향(影響))

  • Jin Jae-Do;Kim Yun-Hong;Jung Tae-Young;Seo Jeong-Chul;Leem Seong-Cheol;Han Sang-Won
    • Korean Journal of Acupuncture
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    • v.20 no.2
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    • pp.77-92
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    • 2003
  • Objective; This study was investigated to test the anti-anemic effects of Danggwibohyeol-tang for herbal acupuncture on rat with anemia induced by phenylhydrazine according to composition ratio. Methods; Danggwibohyeol-tang herbal-acupuncture was administered into Kyoksu(膈兪穴, BL17) and RBC, Hemoglobin, Hematocrit, MCV, MCH, MCHC, RDW, platelet, MPV, PDW, indirect bilirubin and lactate dehydrogenase were tested. Results; The obtained results are summarized as follows: (1) In control group RBC was significantly reduced in comparison with normal group. And in Danggwibohyeol-tang group A, B, C there was significant inhibitory effect on reduction in comparison with control group. The effect of Danggwibohyeol-tang group C was similiar to that of Danggwibohyeol-tang group B and was higher than that of Danggwibohyeol-tang group A. (2) In control group hemoglobin was significantly reduced in comparison with normal group. And in Danggwibohyeol-tang group A, B, C there was significant inhibitory effect on reduction in comparison with control group but there was no significant difference between Danggwibohyeol-tang group A, B, C. (3) In control group hematocrit was significantly reduced in comparison with normal group. And in Danggwibohyeol-tang group A, B, C there was significant inhibitory effect on reduction in comparison with control group. The effect of Danggwibohyeol-tang group C was higher than that of Danggwibohyeol-tang group A or B. (4) In case of MCV, MCH, MCHC and RDW there was no significant anti-anemic effect. (5) Danggwibohyeol-tang group significantly inhibited the growth of platelet. In case of Danggwibohyeol-tang A and B group there was more inhibitory effect than Danggwibohyeol-tang C group but there was no significant difference between Danggwibohyeol-tang A and B group. (6) In case of MPV, PDW and indirect bilirubin there was no significant difference between Danggwibohyeol-tang group and control group. (7) Danggwibohyeol-tang group significantly inhibited the growth of lactate dehydrogenase. In case of Danggwibohyeol-tang C group there was more inhibitory effect than Danggwibohyeol-tang B group and Danggwibohyeol-tang B group showed more inhibitory effect than Danggwibohyeol-tang A group. Conclusion; From above results Danggwibohyeol-tang for herbal acupuncture showed significant anti-anemic effect in some parameters. Further study is needed for the effect of Danggwibohyeol-tang according to different composition ratio.

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