• Bulletin of the Korean Chemical Society
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• 제30권1호
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• pp.197-200
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• 2009

A simple dye having phenol and indole moieties was synthesized and its chromogenic signaling behaviors for the determination of water content in organic solvents were investigated. The compound revealed a pronounced chromogenic behavior in response to the variation of water content in water miscible aprotic organic solvents of acetone, acetonitrile, THF, and dioxane. Significant red shifts and changes in absorption spectra allowed a ratiometric analysis of the signaling behavior. The chemosensing behaviors were particularly pronounced in water content in less than 10% that is suitable for the application of the compound as a probe for the determination of water content in binary aqueous organic solutions having lower water content.

• Mycobiology
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• 제39권2호
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• pp.129-132
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• 2011

To determine the optimal media conditions for the detection of the extracellular cellulase activity in Ganoderma neo-japonicum, we varied three media conditions: dye reagent, pH, and temperature. We evaluated the use of four dyes, Congo red, phenol red, remazol brilliant blue, and trypan blue. To observe the effect of pH on the chromogenic reaction, we tested media ranging from 4.5 to 8.0. To research the effect of temperature on the clear zone and the fungus growing zone, we tested temperatures ranging from 15 to $35^{\circ}C$. On the whole, the best protocol called for Ganoderma neo-japonicum transfer onto media containing Congo red with a pH of 7.0, followed by incubation at $25^{\circ}C$ for 5 days. Our results will be useful to researchers who study extracellular enzyme activity in Ganoderma neo-japonicum.

• Mycobiology
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• 제37권4호
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• pp.313-316
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• 2009

To determine the optimal medium conditions for the detection of the cellulolytic activity in Ganoderma lucidum, we varied three media conditions: dye reagent, pH, and temperature. First, we evaluated the use of four dyes, Congo Red, Phenol Red, Remazol Brilliant Blue, and Trypan Blue. To observe the effect of pH on the chromogenic reaction, we also made and tested various media spanning acidic and alkaline pHs, ranging from 4.5 to 8.0. Furthermore, in order to research the effect of temperature on the clear zone and the fungus growing zone, we tested temperatures ranging from 15 to $35{^{\circ}C}$. On the whole, the best protocol called for Ganoderma lucidum transfer onto media containing Congo red with pH adjusted to 7.0, followed by incubation at $25{^{\circ}C}$ for 5 days. Our results will be useful to researchers who aim to study extracellular enzyme activity in Ganoderma lucidum.

• 한국환경과학회지
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• 제6권1호
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• pp.69-73
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• 1997

Endotoxin from the cell wall of marine V. vujnificus was .extracted using the hot phenol-water method, injected endotoxin into rat, and tested the toxic effect of endotoxin on the blood component In rat blood. The results showed that blood glucose, blood urea nitrogen, white blood cell, and reticulocyte were Increased and red blood cell was the same as the number of control group(normal blood), but platelet was decreased. Above results suggested that endotoxin induced a malfunction of liver and that the Increase of white blood cell was for the removal of foreign toxic substance.

• 보존과학회지
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• 제37권5호
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• pp.606-616
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• 2021

본 연구에서는 한국 고대 붉은 간토기 중 국립김해박물관 소장품인 적색마연완 붉은 간토기(신석기시대), 단도마연 붉은 간토기(청동기시대), 적색마연가지문 붉은 간토기(청동기시대)에 사용된 적색 안료와 교착제를 확인하여 고대 붉은 간토기에 사용된 적색 안료와 교착제의 종류에 대한 자료를 구축하고자 하였다. 과학적 분석 결과, 붉은 간토기 3점 모두 소지층과 적색의 표면층으로 구분되었으며, 적색의 표면층에서는 적색 안료 입자와 광물의 결정이 보였다. SEM-EDS, Raman 분석을 통해 적색 안료는 산화철(Fe₂O₃)을 주 발색원으로 하는 토양성 안료 중 석간주로 추정하였으며, FTIR-ATR을 통해 Urushiol polymer의 특징에 의한 성분 피크가 검출되었다. GC/MS 분석 결과, 적색층에서 옻칠의 방향구조에 기인하는 성분인 Benzenemethanol-2-propenyl, 4-heptyl phenol, 1-Tetradecanol, Heptafluorobutyroxytridecane 성분이 검출되었다. 따라서 국립김해박물관 소장품인 붉은 간토기 3점의 적색 안료는 석간주(Fe₂O₃)를, 교착제로는 옻칠을 사용하여 제작된 토기인 것으로 판단되었다.

• 한국식품영양학회지
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• 제25권4호
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• pp.827-832
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• 2012

본 연구에서는 고온고압 처리를 통한 홍삼 추출물의 항산화 활성과 페놀산 성분의 변화를 측정하였다. 고온고압 처리 홍삼의 총 페놀 함량은 14.76 mg/g으로 기존의 수삼 3.59 mg/g과 홍삼 3.93 mg/g에 비해 높은 함량을 나타냈다. 항산화 활성은 농도 $1{\sim}100mg/m{\ell}$ 농도에서 고온고압 처리 홍삼 4.8~78.4%, 수삼 1.0~47.4%, 홍삼 1.8~56.5%의 DPPH 라디칼 소거 활성을 보였으며, ABTS 라디칼 소거 활성은 고온고압 처리 홍삼 8.9~99.8%, 수삼 3.4~96%, 홍삼 1.2~96.5%를 보였다. 신규 홍삼에서는 maltol, p-hydroxybenzoic acid, caffeic acid, vanillic acid, syringic acid, cinnamic acid, p-coumaric acid, ferulic acid 등의 페놀산 성분이 크게 증가한 것을 확인하였으며, 페놀산의 DPPH 라디칼 소거 활성을 측정한 결과, $10mg/m{\ell}$의 농도에서 고온고압 처리 홍삼 81.5%로 수삼 16.6%와 홍삼 32.9% 보다 높은 항산화 활성을 나타내었다.

• 한국식품조리과학회지
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• 제28권5호
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• pp.623-628
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• 2012

본 연구에서는 가정용 전자레인지를 이용하여 간편하고 신속하게 홍삼을 제조하고 제조된 홍삼의 이화학적 특성을 조사하고자 하였다. 홍삼 제조 방법은 가정용 전자레인지의 '해동기능' 13분(A), 가정용 전자레인지의 '조리기능' 6분(B), 가정용 전자레인지의 '해동기능' 44분(C)로 하였다. 전자레인지로 제조된 홍삼의 외관, 분말의 색, 사포닌 조성, 홍삼 제조 시 용출된 사포닌의 양 등을 기존의 일반 홍삼과 비교하였다. 가정용 전자레인지의 '조리기능' 6분(B)과 '해동기능' 44분(C)으로 만든 홍삼은 일반 홍삼과 유사한 색을 가졌다. 전자레인지에 의한 홍삼 제조 시에는 기존 일반 홍삼 제조에 비해 사포닌 손실이 거의 없었다. 전자레인지 홍삼의 총페놀 함량은 일반 홍삼과 유사하였으며, 전자레인지 홍삼의 진세노사이드 함량은 일반 홍삼보다 높았다. 전자레인지 홍삼(A, B)의 진세노사이드 $Rg_1$, Re, Rf, $Rg_2+Rh_1$, $Rb_1$, Rc, $Rb_2$, $Rb_3$, Rd, $Rg_3$ 함량은 일반 홍삼보다 높았으며, 해동 기능 44분의 홍삼(C)은 진세노사이드 $Rg_1$, Re, $Rg_2+Rh_1$, Rc, $Rb_2$, $Rb_3$, Rd, $Rg_3$의 값이 일반 홍삼보다 높았다. 본 연구에서는 가정용 전자레인지를 이용하여 신속하고 간편하게 고기능성의 홍삼을 만드는 방법을 살펴보았고 이로써 인삼 소비 증진 등이 기대된다.

• 한국식품과학회지
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• 제53권6호
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• pp.815-818
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• 2021

The objective of this study was to develop a differential medium with improved selectivity for the isolation of Bacillus cereus. Mannitol egg yolk polymyxin medium supplemented with D-galactose allowed the differentiation of diarrheal- and emetic-type B. cereus through pH monitoring. The pH of the medium decreased significantly when incubating the emetic-type B. cereus, whereas the pH change was not significant when incubating the diarrheal-type. The addition of pH indicators, such as methyl red and phenol red, to the medium allowed visual differentiation between diarrheal- and emetic-type B. cereus. A solid agar medium was also developed by optimizing the concentrations of medium components such as monosaccharides, agar, egg yolk enrichment, pH indicators, and antibiotics. This study indicates the possibility of applying selective media for the differentiation of diarrheal- and emetic-type B. cereus.

• Bulletin of the Korean Chemical Society
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• 제27권9호
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• pp.1439-1444
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• 2006

A $Cu^{2+}$ ion-selective membrane microelectrode has been fabricated from poly vinyl chloride (PVC) matrix membrane containing a new symmetrical hexadentate Schiff,s base 2-{1-(E)-2-((Z)-2-{(E)-2-[(Z)-1-(2-hydroxyphenyl)ethylidene]hydrazono}-1-methylpropylidene)hydrazono]ethyl}phenol (HDNOS) as a neutral carrier, Potassium tetrakis(4-chlorophenyl) borate (KTpClPB) as an anionic excluder and o-nitrophenyloctyl ether (NPOE) as a plasticizing solvent mediator. The microelectrode displays linear potential response in the concentration range of $1.0\;{\times}\;10^{-5}-1.0\;{\times}\;10^{-11}$ M of $Cu^{2+}$. The microelectrode exhibits a nice Nernstian slope of 25.9 ${\pm}$ 0.3 mV $decade^{-1}$ in the pH range of 3.1-8.1. The sensor has a relatively short response time in whole concentration ranges ($\sim$5 s). The detection limit of proposed sensor is $5.0\;{\times}\;10^{-12}$ M (320 pg/L), and it can be used over a period of eight weeks. The practical utility of the sensor has been demonstrated by using it as an indicator electrode in the potentiometric titration of $Cu^{2+}$ with EDTA. The proposed membrane electrode was used for the direct determining of $Cu^{2+}$ content in black and red pepper, and in waste water samples.

• 대한한의학회지
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• 제20권2호
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• pp.165-176
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• 1999

The in vivo effects of Fructus Benincasae (FB-Baekdongkwa(B) and FB- on the expectoration (decrease in sputum viscoelasticity) by their sorts and using rats (Sp. D. male, $150{\sim}160g$). FB was divided by seed, flesh, and bark, extracted by 95% ethanol for 3 hr. The extracts were given to rats administration and the following results were obtained: 1. When FB Recens-C extract was administered at the concentration of 300 mg/kg b.w., mucus secretion effect in the trachea was desirably stimulated. 2. The secretion of phenol red was increased in the FB-treated tracheas in the order of Semen Benincasae(SB)-B $(153{\pm}8\;%)$, FB-B $(149{\pm}10\;%)$, and FBR-C $(117{\pm}26\;%)$. In general, the effect of FB-B extract on phenol red secretion was stronger than that of FB-C. 3. When tracheobronchial lavage fluid was analyzed, the mucus secretion was relatively high $(111{\pm}14\;%)$ in FB-B compared with other extracts. 4. Microscopic analysis after direct treatment of the FB extracts to the rat tracheal tissue showed that all the FB extracts possessed no effects for the activity of the ciliary movement. 5. Glycoprotein content secreted by the seed extract of FB-B was increased compared with the control group, which represents the highest secretion effect of mucus. From the above results. we could conclude that the seed of SB-B possesses better activity for mucus secretion from trachea than the extracts of any other parts. Therefore, it is expected that the seed of SB-B may be available for the purpose of expectorant activity in the prescription of traditional medicine.