• 한국균학회지
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• 제45권3호
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• pp.246-250
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• 2017

2015년 9월 경상남도농업기술원 시험연구포장에 재배중인 녹두에서 흰비단병 증상이 발생하였다. 병징은 녹두의 줄기 지제 부위가 수침상으로 물러지고 부패되어 서서히 시들면서 포기 전체가 말라 죽었다. 줄기의 병반부와 토양 표면에 흰색의 곰팡이가 발생하며 갈색의 작은 둥근 균핵을 많이 형성하였다. 감자한천배지에서 균총은 흰색이고 잘 자라며 배양기간이 경과됨에 따라 갈색의 작은 둥근 균핵을 많이 형성하였다. 균핵의 크기는 1~3 mm이며 균사의 폭은 $4{\sim}9{\mu}m$였다. 균사 생육과 균핵 형성 적온은 $30^{\circ}C$이었다. 균사 특유의 clamp connection이 관찰되었다. 녹두에서 발생한 병징과 병원균의 균학적 특징, internal transcribed spacer rDNA 염기서열 비교분석 결과를 토대로 이 병을 Sclerotium rolfsii Saccardo에 의한 녹두 흰비단병으로 명명하고자 제안한다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.361-362
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• 2003

본 실험은 생물비료인 인산가용화균 Penicillium sp. PS-113의 어독성과 주요작물에 대한 유해성을 조사하였다. 어독성 실험의 모든 시험구에서 무해한 것으로 판정되었고, 작물생육효과에서도 매우 뛰어난 효과가 있음을 확인할 수 있었다.

• 약학회지
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• 제27권3호
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• pp.221-227
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• 1983

New lectins, lymphoagglutinating lectins from mung beans (MBLA) have been isolated and purified. Mung beans crude extracts were made with 0.15M NaCl and these were purified through anionic exchange chromatography. Four fractions were obtained from DEAE Sephadex A-50 by salt gradients elution. Lectin activity, enzyme activity, protein assay, identification of purity by polyacrylamide gel electrphoresis and immunochemical studies were carried out with these four fractions. Through these results, it can be suggested that 0.2M fraction is newly found potent MBLA. There were some relationships with MBLA and L-PHA but no similarities were observed between MBLA and E-PHA.

• 생약학회지
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• 제30권4호
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• pp.355-362
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• 1999

New lectins have been isolated and purified from mung bean (Phaseolus radiatus) through physiological saline extraction, ammonium sulfate salt fractionation and column chromatographies. Ion exchanger were eluted by linear salt gradient and then further purified through gel filtration. Thus obtained lectin named as MBL. The gene expressions of 5 cytokines (IL-1, IL-2, IL-6, $TNF-{\aphpa}$ and $IFN-{\gamma}$) from human peripheral blood mononuclear cells (PBMC) stimulated with MBL were investigated by using reverse transcription polymerase chain reaction (RT-PCR). PBMC ($1{\times}106$ cells/ml) isolated from healthy volunteers were stimulated with lectins (4 mg/ml) for various time intervals (1 to 96 hrs). After each of the various stimulated times, total RNA was isolated and assessed for different cytokines mRNA by RT-PCR. The mRNA encoding IL-1, IL-2 were detected continuously from 1 to 20 hrs, and IL-6 was detected up to 24 hrs. But the mRNA encoding $IFN-{\gamma}$ and $TNF-{\alpha}$ were detected to 8 hours only and showed short time response compared with other cytokines. The significant expression of all cytokines mRNA were observed at 4 hrs. These results suggested that MBL, as inducer of cytokines could elicit detectable cytokine mRNA from PBMC.

• The Korean Journal of Ecology
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• 제13권4호
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• pp.251-266
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• 1990

Extrafloral nectaries have been shown in many studies to promote mutualistic interactions between plants and insects(usually ants) that visit the glands. The insects gain sugars, water and amino acids secreted by the extrafloral nectaries and benefit the plants by reducing the damage caused by plant's inseet herbivores. Little is known about the occurrence of extrafloral nectaries in plants growing in Asia. To learn about the occurrence of extrafloral nectary bearing plants in Korea, living plants and herbarium material were examined for the glands. In addition, the cover of plants with extrafloral nectaries and the proportion of woody plants with extrafloral nectaries were measured in three forest communities on Kangwha Island. 131 species of plants belonging to 53 genera and 30 families were found to have extrafloral nectaries. These 131 species comprise about 4.0% of Korea's flora, a highet percentage of extrafloral nectary bearing plants than occurs in the studied areas of North America. Extrafloral nectary bearing plants occupied 7, 23 and 55% of the covers and comprised 15, 21 and 15% of the woody plants in the three different forests, a significant level of occurrence. Many important Korean crop plants were found to have extrafloral nectaries including : sesame (Sesamum indicum L.), squash (Cucurbita moschata Duchesne), sweet potato (Ipomoea batatas Lam), persimmon (Diospyros kaki Thunb.) cotton (Gossypium indicum Lam.), mung bean (Phaseolus radiatus L.), red bean (Phaseolus angularis W.F.), peach (Prunus persica (L) Batsch.), plum (Prunus salicina Lindl.). Many of these cultivated and wild plants may receive protection by ants and other beneficial insects that visit their extrafloral nectaries.

• Journal of Plant Biology
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• 제38권4호
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• pp.349-357
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• 1995

The alkaline invertase ($\beta$-D-fructofuranoside fructohydrolase, EC 3.2.1.26) was isolated and characterized from the hypocotyls of mung bean (Phaseolus radiatus L.). The enzyme was purified by consecutive step using diethylaminoethyl (DEAE)-cellulose anion exchange, 1st Sephadex G-200, DEAE-Sephadex A50 and 2nd Sephadex G-200 chromatography. The overall purification was about 77-fold with a yield of about 6%. The finally purified enzyme exhibited a specific activity of about 48 $\mu$mol of glucose produced mg-1 protein min-1 at pH 7.0 and appeared to be a single protein by nondenaturing polyacrylamide gel electrophoresis (PAGE). The enzyme had the native molecular weight of 450 kD and subunits molecular weight of 63 kD and 38 kD as estimated by Sephadex G-200 chromatography and SDS-PAGE, respectively, suggesting that the enzyme is a heteromultimeric protein composed of two types of subunits. On the other hand, the enzyme appeared to be not a glycoprotein according to the results of Con A chromatography and glycoprotein staining. The enzyme had a Km for sucrose of 19.7 mM at pH 7.0 and maximum activity around pH 7.5. The enzyme was most active with sucrose as substrate, compared to raffinose, cellobiose, maltose and lactose. These results indicate the alkaline invertase is a $\beta$-fructofuranosidase.

• 환경생물
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• 제22권2호
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• pp.336-340
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• 2004

In order to search for anti -melanin formation agents from Korean medicinal herbs, we selected 21 Korean medicinal herbs, based on a review of Korean traditional medicine books and the recommendations of Korean traditional medical doctors. We tested for inhibition effect of melanin pigmentation of Clone M-3 mouse melanocyte cell lines when we treated the extracts of 21 medicinal herbs in the mouse melanocyte cell lines, respectively. Among 21 medicinal herb extracts, 5 extracts showed a inhibition effect of melanin formation. The sample Phaseolus radiatus L, Cordyceps militaris, Pinellia ternata, Phellinus linteus and Citrus junos Tanaka showed a significantly little formation of melanin pigments compared with control groups. Especially extract of Citrus junos Tanaka was more potent than the others. These results suggest that extract of Korean Citrus junos may represents an excellent candidate for inhibition of melanin pigmentation at in vitro level.

• 한국식물병리학회지
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• 제11권1호
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• pp.30-38
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• 1995

Anthracnose fungi associated with soybean (Glycine max), small red bean (Vigna angularis), and green bean (Phaseolus radiatus var. aurea) were compared with respect to cultural characteristics, morphological aspects, and pathogenicity. From the legumes, four different species of Colletotrichum were identified based on their morphological characters : C. truncatum, C. destructivum, C. trifolii and C. gloeosporioides. Colonies of Colletotrichum spp.on culture media varied in colour and growth habits depending upon the media and isolates. In general, formation of conidia, setae, and perithecia on water agar leaf medium was better than that on potato dextrose agar medium under near ultraviolet light. In cross inoculation tests, isolates of C. truncatum, c. destructivum, C. trifolii and C. gloeosporioides could infect soybean, small red bean, and green bean at different growth stages. There were some differences in pathogenicity or susceptibility depending on crops tested. C. truncatum was the most virulent and major pathogenic species among the four species.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2005년도 총회 및 춘계학술발표회
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• pp.175-177
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• 2005

Effect of arsenite, arsenate and dimethylarsinic acid (DMA) on the growth of seedling plants were investigated in order to compare the toxicity of arsenic species in soil environments. Test plants were mung bean (Phaseolus radiatus), wheat (Triticum aestivum), barely (Hordeum vulgare), cucumber (Cucumis sativus L.). Seedling growth in As-contaminated soil were significantly reduced in all test species. Arsenite was more toxic than arsenate and DMA. Among the test plants, mung bean was most sensitive to arsenic, followed by cucumber, wheat, and barely.

• 한방안이비인후피부과학회지
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• 제31권4호
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• pp.136-136
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• 2018