• 생명과학회지
• /
• 제11권4호
• /
• pp.362-370
• /
• 2001

Regulation of cell proliferation is a complex process involving the regulated expression and /or modification of discrete gene products. which control transition between different stages of the cycle. The purpose of this short review is to provide an overview of somatic cell cycle events and their controls. Cycline have appeared as major positive regulators in this network, because their association to the cyclin-dependent kinases(Cdks) allows the subsequent activation on the Cdk/cyclin complexes and their catalatic activity. In mammalian cells, early to mid G1 progression and late G1 progression leading to S phase entry are directed by D-type cyclins-Cdk4, 6 and cyclin E-Cdk 2 both of which can phosphorylate the retinoblastoma protein (pRB). pRB is a transcriptional repressor which, in its unphosphorylated state, binds to members of the E2F transcription factor family and blocks E2F-dependent transcription of genes controlling the G1 to S phase transition an subsequent DNA synthesis. Cyclin A is produced in late G1 and expressed during S and G2 phae, and expression of B-type cyclins is typically maximal during the G2 to M phase transition and it controls the passage through M phase. They primarily associate with the activate Cdk2, and Cdc2, respectively. On the other hand, the Cdk inhibitors negatively control the activity of C아/cyclin complex by coordinating internal and/or external signals and impending proliferation at several key checkpoints. These current and further findings will provide novel approaches to understanding and treating major diseases.

• 한국환경교육학회지:환경교육
• /
• 제14권1호
• /
• pp.32-42
• /
• 2001

This study examined the effects of prompts, environmental alteration, and feedback on reduction of food wastes. The study was conducted at a student restaurant at a university. Subjects in this study were college students, faculty members and administrative staffs at the university. An A-BC-BCD-BC within subject design was employed. After baseline phase(A), prompts and environmental alteration were manipulated in the second phase(BC). In the next phase(BCD), feedback was added in addition to the prompts and environmental alteration. In the last phase(B), all the treatments except environmental alteration were withdrawn. The dependent variables n cluded (1) total weight of food waste per day, (2), the weight of food waster per person, (3) the ratio of food waste to total food served per day, (4) total amount of monetary value for food waste to total food served per day. The results indicated that prompts and environmental alteration were affective in reducing food wastes. Thus, measures of all the dependent variables decreased when environmental alteration and prompts were manupulated. However, when feedbacks were added to prompts and environmental alteration, the additional effect of feedback was not observed. The implications and limitations of these findings were discussed and the directions of future studies were also proposed.

• Current Optics and Photonics
• /
• 제1권6호
• /
• pp.579-586
• /
• 2017

In this paper, we theoretically demonstrate that semiconductor optical amplifiers (SOAs) with strong wavelength dependence of gain and phase are capable of all-optical inverted and non-inverted wavelength conversion (WC) over a wide range, with the assistance of an optical filter. First, the gain dynamics and phase dynamics in a common quantum well (QW) SOA with the $In_{0.53}Ga_{0.47}As/In_{0.7322}Ga_{0.2678}As_{0.5810}P_{0.4190}$ material system are found to be strongly dependent on wavelength, which is mainly related to the wavelength dependence of the differential gain and the differential refractive-index change. Second, the wavelength dependence in an all-optical wavelength converter based on the QW SOA cascaded with a detuning band pass filter is studied. Simulations show that the quality of the converted signal has little dependence on the operation wavelength. Both inverted and non-inverted WC can be achieved, over a large wavelength range. Therefore, although the gain and phase change are strongly wavelength-dependent, the effects of this dependence can be erased by appropriate optical filtering.

• 대한기계학회논문집
• /
• 제19권9호
• /
• pp.2181-2193
• /
• 1995

The effective elasticity and conduction of composite materials containing arbitrarily oriented multiple phases has been analyzed using the concept of orientation-dependent average fields and concentration factors. The analysis provided closed form expressions for the effective stiffnesses and conductivities. Under the prescribed boundary conditions, the concentration factors were evaluated by the equivalent inclusion principle, through which the interaction between various phases is approximated by the Mori-Tanaka mean-field approximation. SiC particulate(SiC$_{p}$) reinforce aluminum(Al) matrix composites were fabricated and their elastic constants and electrical conductivities were measured together with a careful study of their microstructure. The measured properties showed a systematic anisotropy and this behavior could be attributed to the preferred orientation of SiC$_{p}$. The theoretical model developed was applied to the computation of the anisotropic properties of these composites. Both two-phase and three-phase composites were considered based on the microstructural information. The SiC$_{p}$ was modeled as an ellipsoid with planar random orientation distribution in the extruded Al/SiC$_{p}$ composites. The effect of extraneous phase such as intermetallic compounds was also investigated.tigated.

• Journal of Photoscience
• /
• 제9권2호
• /
• pp.246-248
• /
• 2002

Light is a major environmental signal for entrainment of the circadian clock, but little is known about the phototransduction pathway triggered by light-activation of photoreceptive molecule(s) responsible for the phase shift of the clock in vertebrates. The chicken pineal gland and retina contain the autonomous circadian oscillators together with the photic entrainment pathway, and hence they provide useful experimental model for the clock system. We previously demonstrated the expression and light-dependent activation of rod-type transducin $\alpha$-subunit (Gtl$\alpha$) in the chicken pineal gland. It is unlikely, however, that the pineal Gt$_1$$\alpha$ plays a major role in the photic entrainment, because the light-induced phase shift is unaffected by bloking the signaling function of Gt$_1$$\alpha$. Here, we show the expression of G 11 $\alpha$, an $\alpha$-subunit of another heterotrimeric G-protein, in the chicken pineal gland and retina by cDNA cloning, Northern blot and Western blot analyses. GIl$\alpha$-immunoreactivity was colocalized with pinopsin in the chicken pineal cells and it was found predominantly at the outer segments of photoreceptor cells in the retinal sections, suggesting functional coupling of G11 $\alpha$ with opsins in the both the tissues. By coimmunoprecipitation experiments using the retina, we showed the light- and GTP-dependent interaction between rhodopsin and G11 $\alpha$. Upon ectopic expression of a Gq/ 11-coupled receptor in cultured pineal cells, pharmacological (non-photic) activation of endogenous G11 induced phase-dependent phase shifts of the melatonin rhythm in a manner very similar to the effect of light. These results suggested opsin-G11 pathway contributing to the photic entrainment of the circadian clock.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 1992년도 하계학술대회 논문집 A
• /
• pp.279-281
• /
• 1992

In this paper, an LQG/LTR procedure for stable nonminimum phase systems is suggested using predictor scheme. In the method, the performance of the target feedback loop can be easily adjusted and the recovery error is less dependent on the location of NMP zeros than previous methods. The gain and phase margin and the robust ness for modeluncertainty of the suggested control system are obtained.

• 전자공학회논문지A
• /
• 제29A권2호
• /
• pp.41-46
• /
• 1992

Mutually pumped phase conjugator(MPPC) is experimentally investigated and analyzed in terms of fanning gratings of a photorefractive BaTiO$_3$ crystal. As considering the intensity-dependent absorption and coupling cofficients in photorefractive two wave mixing, the dependence of phase conjugate reflectivites on the incident angle, beam ratio, and total intensity in MPPC is analyzed.

• 한국환경과학회:학술대회논문집
• /
• 한국환경과학회 2003년도 International Symposium on Clean Environment
• /
• pp.135-138
• /
• 2003

Based on solid phase extraction, gas chromatography and mass spectrometry procedure for determining phenol and its derivatives in natural water was presented. In solid phase extraction, three types of techniques using solid phase adsorption material were treated with acid and salt, and converted second portion of acetyl derivatives. Under the these condition, extraction efficiency and detection ability dependent on extraction methods were discussed. Obtained results using optimized solid phase extraction techniques showed more convenience, simplifier and lower cost than the conventional analytical methods with holding wide dynamic range and lower detection limits.

• 한국정보디스플레이학회:학술대회논문집
• /
• 한국정보디스플레이학회 2009년도 9th International Meeting on Information Display
• /
• pp.942-944
• /
• 2009

Blue phase shows several different reflection colors from the randomly oriented domains and crystal direction. Also there are variations in the size of domains. The domain size is dependent on the temperature gradient. With smaller cooling rate of temperature, the domain size was increased compared with rapid cooling. With injection of light of specific wavelength, we find that the diffraction patterns were occurred around the light spot in the cell of blue phase. It was supposed to be from the matching of the phase retardation and domain size. However, actually the diffraction pattern is reflecting the lattice structure in double twist of the blue phase. The lattice constant from the radius of diffraction patterns shows very similar one from the reflection spectrum, which indicates the internal lattice constant in double twist of the blues phase.

• 생명과학회지
• /
• 제27권3호
• /
• pp.310-317
• /
• 2017

본 연구진은 HaCaT-ARE-luciferase 세포를 이용하여 400 여개의 약용식물 에탄올 추출물 중 NRF2/ARE 유도효과가 있는 신규 추출물을 검색하였고 이를 통하여 종대황(Rheum undulatum)과 선복화(Inula japonica)의 주정 추출물이 HaCaT-ARE-luciferase 세포에서 ARE 활성을 강하게 유도하는 것을 관찰하였다. 종대황과 선복화 에탄올 추출물은 HaCaT 세포에서 생존(viability)을 증가시켰고 NRF2/ARE에 의존적인 phase II cytoprotective 효소인 heme oxygenase-1 (HO-1)와 NADPH:quinone oxidoreductase-1 (NQO1)의 전사 및 단백질 발현을 강하게 유도하였다. 또한 종대황과 선복화 추출물은 HaCaT 세포에서 TPA로 유도한 세포 내 활성 산소 및 이를 통하여 생성되는 스트레스 마커인 8-hydroxydeoxyguanosine (8-OH-dG)과 4-hydroxynonenal (4HNE)의 발생을 강하게 억제하였다. 본 연구는 종대황과 선복화의 에탄올 추출물이 인간 피부 세포주인 HaCaT 세포에서 NRF2/ARE에 의존적인 유전자 발현의 유도를 통하여 강력한 항산화 효과를 발휘한다는 것을 증명한다.