• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.5
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• pp.275-280
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• 2002

In order to control glucose concentration during fed-batch culture for antibiotic production, we applied so called “software sensor” which estimates unmeasured variable of interest from measured process variables using software. All data for analysis were collected from industrial scale cultures in a pharmaceutical company. First, we constructed an estimation model for glucose feed rate to keep glucose concentration at target value. In actual fed-batch culture, glucose concentration was kept at relatively high and measured once a day, and the glucose feed rate until the next measurement time was determined by an expert worker based on the actual consumption rate. Fuzzy neural network (FNN) was applied to construct the estimation model. From the simulation results using this model, the average error for glucose concentration was 0.88 g/L. The FNN model was also applied for a special culture to keep glucose concentration at low level. Selecting the optimal input variables, it was possible to simulate the culture with a low glucose concentration from the data sets of relatively high glucose concentration. Next, a simulation model to estimate time course of glucose concentration during one day was constructed using the on-line measurable process variables, since glucose concentration was only measured off-line once a day. Here, the recursive fuzzy neural network (RFNN) was applied for the simulation model. As the result of the simulation, average error of RFNN model was 0.91 g/L and this model was found to be useful to supervise the fed-batch culture.

• Journal of Physiology & Pathology in Korean Medicine
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• v.25 no.6
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• pp.996-999
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• 2011

This experiment was performed to investigate whether the combined-preparation of crude drugs (The $Well^{TM}$ Preparation, TW), has hypnotic effects and/or enhances pentobarbital-induced sleep behaviors. TW was mixed with water extracts of Ginseng Radix red, Germinated brown rice, cultured mountain ginseng, and 50% ethanol extracts of Longanae Arillus, Nelumbinis Folium and Chrysanthemi Flos. TW (100 mg/kg, p.o.) reduced sleep onset and prolonged sleep time induced by pentobarbital similar to muscimol (0.2 ${\mu}M$), a $GABA_A$ receptor agonist. Also, TW (2 ${\mu}g$/ml) and pentobarbital (2.5 ${\mu}M$) did not affect the chloride influx in primary cultured cerebellar granule cells, respectively, but the combined-treatment of TW (2 ${\mu}g$/ml) and pentobarbital (2.5 ${\mu}M$) increased the chloride influx onto the cells. In conclusion, TW augments pentobarbital-induced sleep behaviors; these effects may result from chloride channel activation.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.31 no.2
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• pp.24-39
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• 2018

Objectives : The purpose of this survey is to examine Korean medical doctor's prescription status of Jaungo. Methods : The questionnaire was distributed via e-mail to Korean medical doctors who were registered in the association of Korean medicine from December 26th 2017 to February 5th 2018. We screened out respondents who didn't meet screening criteria. Results : Total 325 respondents completed answers, and the computerized data were analyzed. 74.7% of respondents were provided Jaungo from outside (extramural herbal dispensaries, pharmaceutical company). Almost all of the respondents (98.2%) were prescribing Jaungo in the form of ointment, and the cream container was the most commonly used container. In chief Indications of Jaungo were skin dryness (83.1%), pruritus (67.7%), burns (64.0%), and atopic dermatitis (60.9%). More than 60% of the respondents were prescribing Jaungo without additional herbs or additives. Most of patients were educated to apply Jaungo thinly to the affected area once or twice a day or frequently. Reason for dissatisfaction about Jaungo of patient were due to staining clothes (59.1%), offensive smell (44.0%), and poor texture (26.5%). Conclusions : Jaungo is a topical herbal agent frequently used in the Korean medicine. This is the first survey figure out current status of topical herbal agent in Korean medical doctors. The result of this study would contribute to progressing treatment method using topical herbal agent.

• Korean Journal of Clinical Pharmacy
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• v.23 no.4
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• pp.300-306
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• 2013

Background: 6-year College of Pharmacy curriculum had started in Korea, and the students in college of pharmacy are supposed to have student practice in the hospital pharmacy, community pharmacy, pharmaceutical company and administration to experience the role of pharmacists in advance. However, despite Korean Association of Pharmacy Education provided its own teaching plan, most Pharmacy Schools and the hospital pharmacy have difficulty in performing the desirable student practice program because they seldom experienced it. So, we reported the student practice program in the hospital pharmacy conducted by Yonsei University College of Pharmacy prior to the other numerous universities and the evaluation of survey on the student pharmacy practice program. Method: Severance Hospital, Gangnam Severance Hospital and National Health Insurance Service Ilsan Hospital took part in the student pharmacy practice program of Yonsei University. Students took 8-week pharmacy practice in Severance Hospital or Gangnam Severance Hospital plus 4-week pharmacy practice in National Health Insurance Service Ilsan Hospital. Also, students had once-a-week presentation class at school. A survey was conducted to evaluate the student practice program. Results: The presentation class was considerably helpful to share their own experiences at different practice sites in different hospitals, but there were some disadvantages that students could not experience the specific pharmacy practice site on the day of once-a-week presentation at school and so on. The results of the survey on the student practice program reported that students were satisfied with the overall practice program and presentation class at school. Also, they answered that the student practice program in the hospital pharmacy was significantly helpful for planning of the future career. Conclusion: Through the performance of the student practice program in Yonsei University, the adjustment of the student practice program in the hospital is planned to provide better experience of practice to the students and the results will be helpful for the student practice in the hospital of the other colleges of pharmacy.

• Korea Journal of Hospital Management
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• v.14 no.4
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• pp.124-148
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• 2009

Objectives : The purpose of this study was to investigate influencing factors on physician's intention to use cyber salesperson(cSP) to promote ethical drugs of pharmaceutical company. We considered influencing factors based on technology acceptance model(TAM) such as usefulness, easy of use, and attitude about cSP, and included trust and joyfulness of cSP. Methods : Measure items were developed from the previous studies. Data were collected from 1,012 physicians by using web-based self-administered structured questionnaire. Results : The major findings were as follows; First, attitude and intention to use cSP were not significantly varied by socio-demographic variables, such as sex, age, location, and Internet surfing time per day. Also, physician's characteristics such as training and professional level and type of employment were not significant to the variation of attitude and intention to use cSP. Second, structural equation modeling(SEM) analysis shows that usefulness and joyfulness were influencing factors of intention to use. Attitude was revealed as significant mediating factor to intention to use. Usefulness has indirect effect on intention to use via attitude. However, trust has not significant effect on intention to use, due to suppression effect. Conclusions : This results implies that cSP will be a useful agent to promote the ethical drugs to physician. Usefulness and joyfulness should be considered as important factors in designing and operating cSP in the Internet.

• BMB Reports
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• v.29 no.3
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• pp.230-235
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• 1996

The interaction of ${\alpha}-ketoglutarate$ dehydrogenase complex (${\alpha}-KGDC$) with a hydrophobic fluorescent probe [1,1'-bi(4-aniline)naphthalene-5,5'-disulfonic acid] (bis-ANS) was studied. The punfied ${\alpha}-KGDC$ was potently inhibited by bis-ANS with an apparent half maximal inhibitory concentration ($IC_{50}$) of 9.8 ${\mu}m$ at pH 8.0. The catalytic activities of both the E1o and E2o subunits were predominantly inhibited while that of the E3 component was hardly affected. The binding of bis-ANS to the enzyme caused a marked enhancement and blue shift from 523 nm to 482 nm in the fluorescence emission spectrum. The dissociation constant ($K_d$) and the number of binding sites (n) were calculated to be 0.87 mM and 158, respectively. Allosteric regulators such as purine nucleotides and divalent cations further increased the fluorescence intensity of the $bis-ANS-{\alpha}-KGDC$ binary complex. These data suggest that the binding of these allosteric regulators to ${\alpha}-KGDC$ may cause the conformational changes in the enzyme and that bis-ANS could be used as a valuable probe to study the interaction of the multi-enzyme complex and its allosteric regulators.

• Korean Journal of Pharmacognosy
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• v.35 no.4 s.139
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• pp.288-292
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• 2004

To evaluate the quality of the crude drugs using three kinds of Cinnamomum Cortex (CC), Vietnamese CC (VCC, the stem bark of Cinnamomum obtusifolium), periderm-peeled Chinese CC (PPCC, periderm-peeled stem bark of C. cassia), Chinese CC (CCC, stem bark of C. cassia) and a Cinnamomi Ramulus (CR, the twig of C. cassia), the four essential oils were prepared by steam distillation method. Cinnamaldehdye (CAN) and an unknown substance tentatively named hydroxy-cinnamaldehdye(HCNA) were detected in the four essential oils by gas chromatography-mass spectrometry, the contents of which are significantly different one another. Vietnamese CC had the highest content of HCNA whereas CR had the highest CAN content and the lowest HCNA. Vietnamese CC exhibited the greatest cytotoxic activity against the cancer cell lines, A549, HepG-2, HL-60, P-388, U-937, and KB and CR the lowest cytotoxicity. Contents of CAN and HCNA in CCC and PPCC are positioned between VCC and CR. These results suggest that measurement of HCNA and cytotoxicity may determine the quality of CC and CR.

• Korean Journal of Pharmacognosy
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• v.37 no.2 s.145
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• pp.110-115
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• 2006

Melittia inouei (Yuri Nabang) larvae are used as a crude drug in East Asia for treating stomach cancer and inflammation, and currently reared as a pharmaceutical insect in Jejudo, Korea. This study evaluated the immuno-modulating activity of these extracts, by determining the level of, cytokine production from mouse splenocytes stimulated with the extracts. The Melittia inouei larvae extracts did not induce the splenocyte proliferation. On the other hand, they stimulated the splenocytes to produce cytokines such as $TNF-{\alpha}$, whereas they did not stimulate IL10, IL12 or $IFN-{\gamma}$. The aqueous portion of its plant (Tri-chosanthis kirilowii) extract (sap) was found to be a potent inducer of NO production from the CPAE cells. However, it showed weak inhibitory effects on vascular endothelial growth factor (VEGF) production from splenocytes. These data suggests that a Melittia inouei larvae extract immune modulatory activity in cytokine prodcutions such as $TNF-{\alpha}$ and VEGF which might be related its anticancer effect.

• The Journal of Internal Korean Medicine
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• v.38 no.6
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• pp.980-999
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• 2017

Objectives: This study aimed to evaluate the efficacy of Daegunjoong-tang for improvement of gastrointestinal motility after surgery due to gastrointestinal cancer by analyzing the existing clinical research. Methods: Clinical studies about Daegunjoong-tang for improvement of gastrointestinal motility after surgery due to gastrointestinal cancer were identified in a literature search using the search term "Daikenchuto AND cancer AND ileus." The studies were analyzed in terms of design, inclusion and exclusion of participants, intervention, control, outcomes, and results. Results: Nine articles were identified in the literature search. Four trials included colon cancer participants with colectomy, and three studies included gastric cancer with total gastrectomy. The intervention in each case was Daegunjoong-tang, and most interventions were made by a pharmaceutical company with a Good Manufacturing Practice facility. The most frequently used control was a placebo. The methods were diverse, including measuring gastrointestinal function, motility, quality of life, symptom scores with a numeric rating scale, and blood tests. Safety was investigated by recording adverse events. Conclusions: Some issues were discovered by reviewing the existing clinical research about Daegunjoong-tang for improvement of gastrointestinal motility after surgery for gastrointestinal cancer. These results will be utilized as evidence for using Daegunjoong-tang in clinical practice and designing a clinical trial for Korean patients.

• Applied Biological Chemistry
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• v.38 no.4
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• pp.293-296
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• 1995

Fig leaves were extracted with methanol and then fractionated with ethyl acetate and various buffers to get active fractions and determined the antimicrobial activities. The acidic and phenolic fractions fractionated from the methanol extract of fig leaves showed the strong antimicrobial activities, but the basic and neutral fractions did not show any activities. The degree of antimicrobial activities of phenolic fraction against tested bacteria was higher than those of acidic fraction, but these against yeasts and mold were almost equivalent to those of acidic fraction. Especially, phenolic fraction was mostly affected on Staphylococcus aureus and Pseudomonas aeruginosa. Four antimicrobial substances purified from the phenolic fraction which showed the strongest antimicrobial activities among the fractions from fig leaves, were identified as psoralen($C_{11}H_{6}O_{3}$, MW. 186), bergapten($C_{12}H_{8}O_{4}$, MW. 216), ${\beta}$-sitosterol($C_{29}H_{50}O$, MW. 414) and umbelliferone ($C_{9}H_{6}O_{3}$, MW. 162).